BR112016004092A2 - glicerol e ácido acético convertendo células de levedura com conversão de ácido acético melhorada - Google Patents

glicerol e ácido acético convertendo células de levedura com conversão de ácido acético melhorada

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Publication number
BR112016004092A2
BR112016004092A2 BR112016004092A BR112016004092A BR112016004092A2 BR 112016004092 A2 BR112016004092 A2 BR 112016004092A2 BR 112016004092 A BR112016004092 A BR 112016004092A BR 112016004092 A BR112016004092 A BR 112016004092A BR 112016004092 A2 BR112016004092 A2 BR 112016004092A2
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Brazil
Prior art keywords
acetic acid
glycerol
yeast cells
nucleotide sequences
improved
Prior art date
Application number
BR112016004092A
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English (en)
Inventor
Klaassen Paul
Willem Antonius Hartman Wouter
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Dsm Ip Assets Bv
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Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of BR112016004092A2 publication Critical patent/BR112016004092A2/pt

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01006—Glycerol dehydrogenase (1.1.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/01—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with NAD+ or NADP+ as acceptor (1.2.1)
    • C12Y102/0101—Acetaldehyde dehydrogenase (acetylating) (1.2.1.10)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01028—Triokinase (2.7.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01029—Glycerone kinase (2.7.1.29), i.e. dihydroxyacetone kinase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y602/00—Ligases forming carbon-sulfur bonds (6.2)
    • C12Y602/01—Acid-Thiol Ligases (6.2.1)
    • C12Y602/01001—Acetate-CoA ligase (6.2.1.1)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

1/1 resumo “glicerol e ácido acético convertendo células de levedura com conversão de ácido acético melhorada” célula que é geneticamente modificada compreendendo: a) uma ou mais sequência de nucleotídeo codificando uma acetaldeído desidrogenase de acetilação dependente de nad+ (e.c. 1.2.1.10); b) uma ou mais sequência de nucleotídeo codificando uma acetil-coa sintetase (e.c. 6.2.1.1); c) uma ou mais sequência de nucleotideo codificando uma glicerol desidrogenase (e.c. 1.1.1.6); e d) uma ou mais sequência de nucleotideo codificando uma dihidroxiacetona cinase (e.c. 2.7.1.28 ou e.c. 2.7.1.29).
BR112016004092A 2013-08-29 2014-08-29 glicerol e ácido acético convertendo células de levedura com conversão de ácido acético melhorada BR112016004092A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13182222 2013-08-29
PCT/EP2014/068324 WO2015028582A2 (en) 2013-08-29 2014-08-29 Glycerol and acetic acid converting yeast cells with improved acetic acid conversion

Publications (1)

Publication Number Publication Date
BR112016004092A2 true BR112016004092A2 (pt) 2017-10-17

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BR112016004092A BR112016004092A2 (pt) 2013-08-29 2014-08-29 glicerol e ácido acético convertendo células de levedura com conversão de ácido acético melhorada

Country Status (10)

Country Link
US (2) US10450588B2 (pt)
EP (2) EP3467104A3 (pt)
KR (1) KR20160046329A (pt)
CN (1) CN105492599A (pt)
AR (1) AR097480A1 (pt)
BR (1) BR112016004092A2 (pt)
DK (1) DK3039130T3 (pt)
MY (1) MY173141A (pt)
PL (1) PL3039130T3 (pt)
WO (1) WO2015028582A2 (pt)

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MY186983A (en) 2015-05-22 2021-08-26 Dsm Ip Assets Bv Acetate consuming yeast cell
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JP7078900B2 (ja) 2018-10-05 2022-06-01 トヨタ自動車株式会社 形質転換酵母及びこれを用いたエタノールの製造方法
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WO2023285297A1 (en) 2021-07-12 2023-01-19 Dsm Ip Assets B.V. Recombinant yeast cell
CN118215740A (zh) 2021-11-04 2024-06-18 丹尼斯科美国公司 用于生产乙醇的方法和重组酵母细胞
CN118176296A (zh) 2021-11-04 2024-06-11 丹尼斯科美国公司 重组酵母细胞
EP4426823A1 (en) 2021-11-04 2024-09-11 Danisco Us Inc Variant polypeptide and recombinant yeast cell
EP4365192A1 (en) 2022-11-04 2024-05-08 DSM IP Assets B.V. Microbial production of growth factors

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Also Published As

Publication number Publication date
EP3467104A2 (en) 2019-04-10
US20160208291A1 (en) 2016-07-21
US11753659B2 (en) 2023-09-12
EP3039130A2 (en) 2016-07-06
EP3039130B1 (en) 2018-12-05
CN105492599A (zh) 2016-04-13
PL3039130T3 (pl) 2019-05-31
US10450588B2 (en) 2019-10-22
AR097480A1 (es) 2016-03-16
WO2015028582A3 (en) 2015-05-21
DK3039130T3 (en) 2019-04-01
EP3467104A3 (en) 2019-05-08
KR20160046329A (ko) 2016-04-28
MY173141A (en) 2019-12-31
US20190330664A1 (en) 2019-10-31
WO2015028582A2 (en) 2015-03-05

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B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
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B350 Update of information on the portal [chapter 15.35 patent gazette]