BR112015023146A2 - levedura recombinante e método para produzir etanol com uso da mesma - Google Patents

levedura recombinante e método para produzir etanol com uso da mesma

Info

Publication number
BR112015023146A2
BR112015023146A2 BR112015023146A BR112015023146A BR112015023146A2 BR 112015023146 A2 BR112015023146 A2 BR 112015023146A2 BR 112015023146 A BR112015023146 A BR 112015023146A BR 112015023146 A BR112015023146 A BR 112015023146A BR 112015023146 A2 BR112015023146 A2 BR 112015023146A2
Authority
BR
Brazil
Prior art keywords
recombinant yeast
xylose
producing ethanol
yeast
isomerase gene
Prior art date
Application number
BR112015023146A
Other languages
English (en)
Other versions
BR112015023146B1 (pt
Inventor
Ishida Nobuhiro
Tada Nobuki
Nagura Risa
Katahira Satoshi
Onishi Toru
Original Assignee
Toyota Motor Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Toyota Motor Co Ltd filed Critical Toyota Motor Co Ltd
Publication of BR112015023146A2 publication Critical patent/BR112015023146A2/pt
Publication of BR112015023146B1 publication Critical patent/BR112015023146B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C12N9/92—Glucose isomerase (5.3.1.5; 5.3.1.9; 5.3.1.18)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01086—Ketol-acid reductoisomerase (1.1.1.86)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y503/00—Intramolecular oxidoreductases (5.3)
    • C12Y503/01—Intramolecular oxidoreductases (5.3) interconverting aldoses and ketoses (5.3.1)
    • C12Y503/01005—Xylose isomerase (5.3.1.5)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

resumo patente de invenção: "levedura recombinante e método para produzir etanol com uso da mesma". a invenção tem como objetivo aprimorar a capacidade de assimilação de xilose e capacidade de fermentação de etanol em uma levedura que assimila xilose na qual um gene xilose isomerase foi introduzido. a quantidade de nadh produzido pela levedura recombinante na qual o gene xilose isomerase foi introduzido como resultado da reação enzimática de ácido hidroxiacético reductoisomerase é diminuída. 1/1
BR112015023146-2A 2013-06-13 2014-06-09 Levedura recombinante e método para produzir etanol com uso da mesma BR112015023146B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2013124755A JP5845210B2 (ja) 2013-06-13 2013-06-13 組換え酵母、及びそれを用いたエタノールの製造方法
JP2013-124755 2013-06-13
PCT/JP2014/003069 WO2014199623A1 (en) 2013-06-13 2014-06-09 Recombinant yeast and method for producing ethanol using the same

Publications (2)

Publication Number Publication Date
BR112015023146A2 true BR112015023146A2 (pt) 2017-11-21
BR112015023146B1 BR112015023146B1 (pt) 2022-11-29

Family

ID=51059504

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015023146-2A BR112015023146B1 (pt) 2013-06-13 2014-06-09 Levedura recombinante e método para produzir etanol com uso da mesma

Country Status (7)

Country Link
US (1) US10017788B2 (pt)
JP (1) JP5845210B2 (pt)
CN (1) CN105008527B (pt)
BR (1) BR112015023146B1 (pt)
CA (1) CA2912037C (pt)
MY (1) MY169263A (pt)
WO (1) WO2014199623A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6879111B2 (ja) * 2017-08-02 2021-06-02 トヨタ自動車株式会社 組換え酵母及びこれを用いたエタノールの製造方法
CN111154743B (zh) * 2020-04-01 2021-01-05 黑龙江省农业科学院畜牧兽医分院 突变型纤维素酶及其在玉米青贮中的应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE60325457D1 (de) 2002-01-23 2009-02-05 Royal Nedalco B V Fermentation von pentosezuckern
SE0200857D0 (sv) 2002-03-19 2002-03-19 Forskarpatent I Syd Ab Metabolic engineering for improved xylose utilisation of saccharomyces cerevisiae
JP2009034036A (ja) 2007-08-01 2009-02-19 Toyota Motor Corp 形質転換用酵母、形質転換方法及び物質生産方法
EP2222841B1 (en) * 2007-12-20 2015-08-26 Butamax (TM) Advanced Biofuels LLC Ketol-acid reductoisomerase using nadh
WO2010051527A2 (en) 2008-10-31 2010-05-06 Gevo, Inc. Engineered microorganisms capable of producing target compounds under anaerobic conditions
JP5608999B2 (ja) 2009-04-08 2014-10-22 株式会社豊田中央研究所 キシロースを利用して有用物質を生産する方法
WO2010151525A1 (en) * 2009-06-22 2010-12-29 Gevo, Inc. Yeast organisms for the production of isobutanol
WO2011078262A1 (ja) * 2009-12-22 2011-06-30 株式会社豊田中央研究所 キシロースイソメラーゼ及びその利用
WO2013048599A2 (en) * 2011-06-16 2013-04-04 Gevo, Inc. Ketol-acid reductoisomerases with improved performance properties

Also Published As

Publication number Publication date
BR112015023146B1 (pt) 2022-11-29
CN105008527B (zh) 2018-06-01
US20160122784A1 (en) 2016-05-05
JP2015000007A (ja) 2015-01-05
CN105008527A (zh) 2015-10-28
MY169263A (en) 2019-03-20
JP5845210B2 (ja) 2016-01-20
US10017788B2 (en) 2018-07-10
CA2912037C (en) 2018-05-15
CA2912037A1 (en) 2014-12-18
WO2014199623A1 (en) 2014-12-18

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Legal Events

Date Code Title Description
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 09/06/2014, OBSERVADAS AS CONDICOES LEGAIS