EA033293B1 - Рекомбинантный микроорганизм, экспрессирующий аналог авермектина, и его применение - Google Patents

Рекомбинантный микроорганизм, экспрессирующий аналог авермектина, и его применение

Info

Publication number
EA033293B1
EA033293B1 EA201691754A EA201691754A EA033293B1 EA 033293 B1 EA033293 B1 EA 033293B1 EA 201691754 A EA201691754 A EA 201691754A EA 201691754 A EA201691754 A EA 201691754A EA 033293 B1 EA033293 B1 EA 033293B1
Authority
EA
Eurasian Patent Office
Prior art keywords
avermectin
recombinant microorganism
analogues
analogue
microorganism expressing
Prior art date
Application number
EA201691754A
Other languages
English (en)
Other versions
EA201691754A1 (ru
Inventor
Цзюнь Хуан
Цзидун Ван
Аньлян Чэнь
Айвэнь Дэн
Цзятань Линь
Чжэнь Юй
Мэйхун Ли
На Ли
Хайбинь Ван
Линхуэй Чжэн
Хуа Бай
Original Assignee
Чжэцзян Хисун Фармасьютикал Ко., Лтд.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Чжэцзян Хисун Фармасьютикал Ко., Лтд. filed Critical Чжэцзян Хисун Фармасьютикал Ко., Лтд.
Publication of EA201691754A1 publication Critical patent/EA201691754A1/ru
Publication of EA033293B1 publication Critical patent/EA033293B1/ru

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
    • C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
    • C12P19/623—Avermectin; Milbemycin; Ivermectin; C-076
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Agronomy & Crop Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Pest Control & Pesticides (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Saccharide Compounds (AREA)

Abstract

Настоящее изобретение относится к рекомбинантному микроорганизму, экспрессирующему авермектин или его аналоги, и к способу его конструирования, а также относится к способу продуцирования авермектина или его аналогов с использованием рекомбинантного микроорганизма и к авермектину или его аналогам, полученным с использованием данного способа. Кроме того, настоящее изобретение относится к применению авермектина или его аналогов в качестве инсектицидов. Использование рекомбинантного микроорганизма по настоящему изобретению для продуцирования авермектина или его аналогов имеет множество преимуществ, например, включающих по меньшей мере одно из следующего: хорошую стабильность, высокий выход продукта, простой способ, безопасность для окружающей среды и значительную экономию производственных затрат.
EA201691754A 2014-03-10 2014-04-28 Рекомбинантный микроорганизм, экспрессирующий аналог авермектина, и его применение EA033293B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201410085431 2014-03-10
PCT/CN2014/076372 WO2015135242A1 (zh) 2014-03-10 2014-04-28 表达阿维菌素类似物的重组微生物及其用途

Publications (2)

Publication Number Publication Date
EA201691754A1 EA201691754A1 (ru) 2016-12-30
EA033293B1 true EA033293B1 (ru) 2019-09-30

Family

ID=54070849

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201691754A EA033293B1 (ru) 2014-03-10 2014-04-28 Рекомбинантный микроорганизм, экспрессирующий аналог авермектина, и его применение

Country Status (12)

Country Link
US (1) US10334850B2 (ru)
EP (1) EP3118305B1 (ru)
JP (1) JP6346673B2 (ru)
CN (2) CN106459885B (ru)
AU (1) AU2014386509B2 (ru)
CA (1) CA2940016A1 (ru)
CL (1) CL2016002223A1 (ru)
EA (1) EA033293B1 (ru)
IL (1) IL247596B (ru)
MX (1) MX2016011367A (ru)
UA (1) UA116835C2 (ru)
WO (1) WO2015135242A1 (ru)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017052232A1 (ko) * 2015-09-22 2017-03-30 주식회사 팜한농 밀베마이신을 생산하는 재조합 미생물 및 이를 이용한 밀베마이신 생산
EP3613842B1 (en) * 2017-03-13 2022-10-19 Zhejiang Hisun Pharmaceutical Co., Ltd. Ivermectin b1b producing strain and use thereof
CN108660101B (zh) * 2017-04-01 2021-07-09 浙江海正药业股份有限公司 表达天维菌素b的重组微生物、制备方法及其用途
CN107494559B (zh) * 2017-08-29 2020-09-29 浙江农林大学 防治柑桔潜叶蛾的杀虫组合物及其用途
CN107509735B (zh) * 2017-08-29 2020-09-11 浙江农林大学 增效杀螨组合物及其用途
CN110540556B (zh) * 2018-05-28 2023-04-28 深圳市天维生物药业有限公司 一种分离和提纯天维菌素的方法
CN110540944B (zh) * 2018-05-28 2022-08-02 深圳市天维生物药业有限公司 一种天维菌素产生菌及其应用
CN110540567B (zh) * 2018-05-28 2024-07-30 深圳市天维生物药业有限公司 一种天维菌素b的晶型及其制备方法和用途
CN110305818A (zh) * 2019-08-15 2019-10-08 齐鲁制药（内蒙古）有限公司 一种阿维菌素菌种选育方法
CN114931144A (zh) * 2022-06-21 2022-08-23 台州科技职业学院 一种大环内酯类化合物及其在防治农林害虫和害螨中的应用

Citations (1)

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CN103834605A (zh) * 2012-11-21 2014-06-04 中国科学院上海有机化学研究所 一种阿维菌素产生菌及其制备方法和应用

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US4134973A (en) * 1977-04-11 1979-01-16 Merck & Co., Inc. Carbohydrate derivatives of milbemycin and processes therefor
EP0235085A1 (de) * 1986-02-20 1987-09-02 Ciba-Geigy Ag 13-Beta-Zuckerderivate von Milbemycinen, deren Herstellung und Verwendung gegen Ekto-und Endoparasiten am Nutztier oder an der Nutzpflanze
US5234831A (en) * 1987-01-23 1993-08-10 Pfizer Inc Cultures for production of B avermectins
DE4031039A1 (de) * 1989-10-03 1991-04-11 Ciba Geigy Ag 13(alpha)-zuckerderivate von milbemycinen, deren herstellung und verwendung gegen ekto- und endoparasiten am nutztier
JP2888586B2 (ja) * 1990-03-05 1999-05-10 社団法人北里研究所 エバーメクチンの特定成分を選択生産するための微生物およびその選択的製造法
US8796230B2 (en) * 2010-01-22 2014-08-05 University Of Southern California Ivermectin antagonizes ethanol inhibition in P2X4 receptors
CN104910228B (zh) * 2014-03-10 2018-12-07 浙江农林大学 十六元大环内酯类化合物及其应用

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103834605A (zh) * 2012-11-21 2014-06-04 中国科学院上海有机化学研究所 一种阿维菌素产生菌及其制备方法和应用

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Title
ZHOU, Haijiao et al., "The Construction of Gene-engineered Strain Only Producing Avermectin B", JOURNAL OF SHENYANG PHARMACEUTICAL, no. 11, vol. 27, 30 November 2010 (30.11.2010), pages 919-923, see abstract, and figure 6 *
ZHU, Haojun et al., "Cloning, Sequence Analysis and Gene Replacement of an Avermectin C5 - O methyltransferase Gene", CHINESE JOURNAL OF ANTIBIOTICS, no. 1, vol. 28, 31 January 2003 (31.01.2003), pages 1-5 *
ZHU, Haojun et al., "Cloning, Sequence Analysis and Gene Replacement of an Avermectin C5 - O methyltransferase Gene", CHINESE JOURNAL OF ANTIBIOTICS, no. 1, vol. 28, 31 January 2003 (31.01.2003), pages 1-5, see abstract, and figure 10 *

Also Published As

Publication number Publication date
US10334850B2 (en) 2019-07-02
MX2016011367A (es) 2017-02-02
AU2014386509B2 (en) 2018-07-05
EP3118305B1 (en) 2019-08-14
UA116835C2 (uk) 2018-05-10
CL2016002223A1 (es) 2017-04-07
EP3118305A1 (en) 2017-01-18
EP3118305A4 (en) 2018-03-21
IL247596A0 (en) 2016-11-30
IL247596B (en) 2019-05-30
JP2017512465A (ja) 2017-05-25
AU2014386509A1 (en) 2016-09-22
US20170013837A1 (en) 2017-01-19
EA201691754A1 (ru) 2016-12-30
CN106459885A (zh) 2017-02-22
CN106795196A (zh) 2017-05-31
WO2015135242A1 (zh) 2015-09-17
JP6346673B2 (ja) 2018-06-20
CN106459885B (zh) 2019-07-23
CA2940016A1 (en) 2015-09-17

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Legal Events

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PC4A Registration of transfer of a eurasian patent by assignment