BR112015022292A2 - processo de fabricação escalonável para produzir vetores lentivirais recombinantes em um sistema de cultura celular em suspensão sem soro - Google Patents

processo de fabricação escalonável para produzir vetores lentivirais recombinantes em um sistema de cultura celular em suspensão sem soro

Info

Publication number
BR112015022292A2
BR112015022292A2 BR112015022292A BR112015022292A BR112015022292A2 BR 112015022292 A2 BR112015022292 A2 BR 112015022292A2 BR 112015022292 A BR112015022292 A BR 112015022292A BR 112015022292 A BR112015022292 A BR 112015022292A BR 112015022292 A2 BR112015022292 A2 BR 112015022292A2
Authority
BR
Brazil
Prior art keywords
manufacturing process
cell culture
culture system
serum free
lentiviral vectors
Prior art date
Application number
BR112015022292A
Other languages
English (en)
Portuguese (pt)
Inventor
Qu Guang
Fraser Wright John
Original Assignee
Childrens Hospital Philadelphia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Childrens Hospital Philadelphia filed Critical Childrens Hospital Philadelphia
Publication of BR112015022292A2 publication Critical patent/BR112015022292A2/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15041—Use of virus, viral particle or viral elements as a vector
    • C12N2740/15043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/15011—Lentivirus, not HIV, e.g. FIV, SIV
    • C12N2740/15051—Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Manufacturing & Machinery (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
BR112015022292A 2013-03-15 2014-03-17 processo de fabricação escalonável para produzir vetores lentivirais recombinantes em um sistema de cultura celular em suspensão sem soro BR112015022292A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361787818P 2013-03-15 2013-03-15
PCT/US2014/030370 WO2014145578A1 (en) 2013-03-15 2014-03-17 Scalable manufacturing process to produce recombinant lentiviral vectors in serum-free suspension cell culture system

Publications (1)

Publication Number Publication Date
BR112015022292A2 true BR112015022292A2 (pt) 2017-07-18

Family

ID=51538029

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015022292A BR112015022292A2 (pt) 2013-03-15 2014-03-17 processo de fabricação escalonável para produzir vetores lentivirais recombinantes em um sistema de cultura celular em suspensão sem soro

Country Status (16)

Country Link
US (2) US20140323556A1 (cg-RX-API-DMAC7.html)
EP (1) EP2970920B1 (cg-RX-API-DMAC7.html)
JP (1) JP6480910B6 (cg-RX-API-DMAC7.html)
KR (1) KR102200278B1 (cg-RX-API-DMAC7.html)
CN (2) CN105378074A (cg-RX-API-DMAC7.html)
AU (1) AU2014232879B2 (cg-RX-API-DMAC7.html)
BR (1) BR112015022292A2 (cg-RX-API-DMAC7.html)
CA (1) CA2904366C (cg-RX-API-DMAC7.html)
DK (1) DK2970920T3 (cg-RX-API-DMAC7.html)
ES (1) ES2681434T3 (cg-RX-API-DMAC7.html)
IL (1) IL241058B (cg-RX-API-DMAC7.html)
MX (1) MX364690B (cg-RX-API-DMAC7.html)
PT (1) PT2970920T (cg-RX-API-DMAC7.html)
RU (1) RU2709678C2 (cg-RX-API-DMAC7.html)
WO (1) WO2014145578A1 (cg-RX-API-DMAC7.html)
ZA (1) ZA201506478B (cg-RX-API-DMAC7.html)

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CN111763238B (zh) * 2019-04-02 2022-07-29 普莱柯生物工程股份有限公司 一种蛋白抗原的纯化方法、制备的蛋白抗原及其应用
ES2965341T3 (es) 2019-04-11 2024-04-12 Regenxbio Inc Métodos de cromatografía de exclusión por tamaño para la caracterización de composiciones de virus adenoasociados recombinantes
CN110054678B (zh) * 2019-05-16 2023-04-18 中国医学科学院血液病医院（血液学研究所） 一种膜结合型mFLT3LG蛋白及其应用
AU2020325705B2 (en) * 2019-08-05 2026-02-05 Sartorius Polyplus Compositions for transfecting a nucleic acid molecule into a cell comprising heterocyclic compounds grafted to a cationic polymer, and their applications
KR20220047635A (ko) 2019-08-23 2022-04-18 론자 워커스빌 아이엔씨. 렌티바이러스 벡터의 생산을 위한 방법 및 작제물
CN110894494B (zh) * 2019-11-22 2022-09-27 广西梧州制药（集团）股份有限公司 一种大规模高密度悬浮培养293细胞高产腺病毒的方法
CN110938654A (zh) * 2019-12-11 2020-03-31 和元生物技术（上海）股份有限公司 一种细胞转染试剂及其应用
CN113122501B (zh) * 2019-12-30 2023-06-16 重庆精准生物技术有限公司 适合大规模临床级病毒载体制备的培养基及其应用
CA3194520A1 (en) * 2020-06-11 2021-12-16 2Seventy Bio, Inc. Methods for manufacturing viral vectors
CA3188923A1 (en) * 2020-08-13 2022-02-17 Michael Sean BURNHAM Methods for producing clinical-grade lentiviral vector
CN112746076B (zh) * 2020-12-28 2023-05-12 中吉智药(南京)生物技术有限公司 一种密码子优化的col7a1基因及慢病毒和应用
WO2022143623A1 (zh) * 2020-12-29 2022-07-07 江苏金斯瑞蓬勃生物科技有限公司 高分散性的hek293t细胞株及其筛选方法
AR125468A1 (es) * 2021-04-27 2023-07-19 Novartis Ag Sistema de producción de vectores virales
EP4373951A1 (en) * 2021-07-19 2024-05-29 2seventy bio, Inc. Vector manufacturing processes
CN113980916B (zh) * 2021-09-27 2024-11-05 湖南丰晖生物科技有限公司 一种慢病毒的纯化方法
US20240425880A1 (en) * 2021-10-12 2024-12-26 Jiangsu Genscript Probio Biotech Co., Ltd. Hek293 cell line adapted to serum-free suspension culture and use thereof
CN114990077A (zh) * 2022-08-01 2022-09-02 深圳市先康达生命科学有限公司 一种慢病毒包装与纯化方法
CN116240241A (zh) * 2022-12-30 2023-06-09 上海本导基因技术有限公司 重组慢病毒载体的纯化制备方法
IN202321021121A (cg-RX-API-DMAC7.html) * 2023-03-24 2024-04-19
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CN122012416A (zh) * 2026-04-14 2026-05-12 山东丽山生物科技有限公司 一种提高慢病毒滴度的方法

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Also Published As

Publication number Publication date
JP6480910B6 (ja) 2019-04-03
RU2709678C2 (ru) 2019-12-19
RU2015144057A (ru) 2017-04-27
AU2014232879B2 (en) 2020-03-05
MX2015012450A (es) 2016-04-25
EP2970920A1 (en) 2016-01-20
CN118256453A (zh) 2024-06-28
WO2014145578A1 (en) 2014-09-18
CA2904366C (en) 2023-07-04
AU2014232879A1 (en) 2015-09-24
US10994028B2 (en) 2021-05-04
IL241058B (en) 2020-05-31
KR102200278B1 (ko) 2021-01-07
CN105378074A (zh) 2016-03-02
MX364690B (es) 2019-05-06
JP2016512700A (ja) 2016-05-09
US20140323556A1 (en) 2014-10-30
IL241058A0 (en) 2015-11-30
DK2970920T3 (en) 2018-08-06
EP2970920A4 (en) 2016-09-14
HK1219978A1 (en) 2017-04-21
US20170368201A1 (en) 2017-12-28
EP2970920B1 (en) 2018-04-25
KR20160024841A (ko) 2016-03-07
CA2904366A1 (en) 2014-09-18
JP6480910B2 (ja) 2019-03-13
PT2970920T (pt) 2018-07-30
ES2681434T3 (es) 2018-09-13
ZA201506478B (en) 2021-09-29

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Legal Events

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001, QUE MODIFICOU A LEI NO 9279/96, A CONCESSAO DA PATENTE ESTA CONDICIONADA A ANUENCIA PREVIA DA ANVISA. CONSIDERANDO A APROVACAO DOS TERMOS DO PARECER NO 337/PGF/EA/2010, BEM COMO A PORTARIA INTERMINISTERIAL NO 1065 DE 24/05/2012, ENCAMINHA-SE O PRESENTE PEDIDO PARA AS PROVIDENCIAS CABIVEIS.

B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B12B Appeal against refusal [chapter 12.2 patent gazette]