BR112014029846A2 - método de inativação da célula hospedeira microbiana em um caldo de fermentação - Google Patents

método de inativação da célula hospedeira microbiana em um caldo de fermentação

Info

Publication number
BR112014029846A2
BR112014029846A2 BR112014029846A BR112014029846A BR112014029846A2 BR 112014029846 A2 BR112014029846 A2 BR 112014029846A2 BR 112014029846 A BR112014029846 A BR 112014029846A BR 112014029846 A BR112014029846 A BR 112014029846A BR 112014029846 A2 BR112014029846 A2 BR 112014029846A2
Authority
BR
Brazil
Prior art keywords
host cell
fermentation broth
microbial host
inactivation method
cell inactivation
Prior art date
Application number
BR112014029846A
Other languages
English (en)
Inventor
Munthe Jensen Helene
Ellegaard Martin
Jakobsen Sune
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of BR112014029846A2 publication Critical patent/BR112014029846A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

1 / 1 resumo “mã‰todo de inativaã‡ãƒo da cã‰lula hospedeira microbiana em um caldo de fermentaã‡ãƒoâ€� um mã©todo de inativaã§ã£o da cã©lula hospedeira microbiana em um caldo de fermentaã§ã£o compreendendo uma enzima de interesse e a cã©lula hospedeira microbiana produtora da enzima de interesse, que compreende: a) adicionar ao caldo de fermentaã§ã£o um ã¡cido graxo com um comprimento da cadeia de c4-c12; e b) misturar o caldo de fermentaã§ã£o por um perã­odo de tempo suficiente atã© a cã©lula hospedeira microbiana ser inativada.
BR112014029846A 2012-07-06 2013-07-02 método de inativação da célula hospedeira microbiana em um caldo de fermentação BR112014029846A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12175293 2012-07-06
PCT/EP2013/063928 WO2014006040A1 (en) 2012-07-06 2013-07-02 Inactivation of a production strain using a fatty acid

Publications (1)

Publication Number Publication Date
BR112014029846A2 true BR112014029846A2 (pt) 2017-06-27

Family

ID=48703590

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014029846A BR112014029846A2 (pt) 2012-07-06 2013-07-02 método de inativação da célula hospedeira microbiana em um caldo de fermentação

Country Status (5)

Country Link
US (1) US20150203807A1 (pt)
EP (1) EP2870256A1 (pt)
CN (1) CN104411832A (pt)
BR (1) BR112014029846A2 (pt)
WO (1) WO2014006040A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111565572A (zh) * 2017-09-29 2020-08-21 0903608 B.C.有限公司 协同农药组合物和用于递送活性成分的方法
CN113286513A (zh) 2018-09-27 2021-08-20 0903608Bc有限公司 协同农药组合物和用于递送杀昆虫活性成分的方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1187512A (en) * 1966-08-05 1970-04-08 Kyowa Hakko Kogyo Kk Process for Extracting Proteins from Micro-Organisms
JPS5534046A (en) 1978-09-01 1980-03-10 Cpc International Inc Novel glucoamyrase having excellent heat resistance and production
NO840200L (no) 1983-01-28 1984-07-30 Cefus Corp Glukoamylase cdna.
DK135983D0 (da) 1983-03-25 1983-03-25 Novo Industri As Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse
US4536477A (en) 1983-08-17 1985-08-20 Cpc International Inc. Thermostable glucoamylase and method for its production
US4587215A (en) 1984-06-25 1986-05-06 Uop Inc. Highly thermostable amyloglucosidase
US4628031A (en) 1984-09-18 1986-12-09 Michigan Biotechnology Institute Thermostable starch converting enzymes
JPS62126989A (ja) 1985-11-26 1987-06-09 Godo Shiyusei Kk コルテイシウム属担子菌の生産する酵素を用いた澱粉質の無蒸煮糖化法
DK0477280T3 (pt) * 1989-06-13 1997-02-10 Genencor Int
IL95019A0 (en) * 1989-08-09 1991-06-10 Mycogen Corp Process for encapsulation of biologicals
ES2250969T3 (es) 1994-03-29 2006-04-16 Novozymes A/S Amilasa alcalina de bacilo.
CA2211316C (en) 1995-02-03 2013-10-01 Novo Nordisk A/S Method of designing alpha-amylase mutants with predetermined properties
CN100351386C (zh) 1997-02-20 2007-11-28 Dsm公司 用化学上确定的培养基以工业规模发酵生产有价值的化合物
DE69840512D1 (en) 1997-11-26 2009-03-12 Novozymes As Thermostabile glukoamylase
DE69942995D1 (de) 1998-02-27 2011-01-13 Novozymes As Maltogene alpha-amylase varianten
PT1294371E (pt) * 2000-06-20 2005-05-31 Nutrition Sciences Acidos gordos de cadeia media utilizaveis como agentes anti-microbianos
MX2007014229A (es) 2005-05-19 2008-02-07 Novozymes North America Inc Produccion de enzimas.
JP5663497B2 (ja) * 2009-02-20 2015-02-04 ダニスコ・ユーエス・インク 培養液製剤
DK2622069T3 (en) * 2010-10-01 2016-02-22 Novozymes Inc Beta-glucosidasevarianter and polynucleotides encoding them

Also Published As

Publication number Publication date
WO2014006040A1 (en) 2014-01-09
EP2870256A1 (en) 2015-05-13
US20150203807A1 (en) 2015-07-23
CN104411832A (zh) 2015-03-11

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA.

B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]