BR112012030583A2 - bactéria, e, método para produzir um l-aminoácido. - Google Patents

bactéria, e, método para produzir um l-aminoácido.

Info

Publication number
BR112012030583A2
BR112012030583A2 BR112012030583A BR112012030583A BR112012030583A2 BR 112012030583 A2 BR112012030583 A2 BR 112012030583A2 BR 112012030583 A BR112012030583 A BR 112012030583A BR 112012030583 A BR112012030583 A BR 112012030583A BR 112012030583 A2 BR112012030583 A2 BR 112012030583A2
Authority
BR
Brazil
Prior art keywords
producing
amino acid
bacteria
bacterium
genes
Prior art date
Application number
BR112012030583A
Other languages
English (en)
Other versions
BR112012030583B1 (pt
Inventor
Dmitriy Vladimirovich Filippov
Elvira Borisovna Voroshilova
Keiko Noguchi
Mika Moriya
Mikhail Markovich Gusyatiner
Tatyana Viktorovna Leonova
Yuri Nagai
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Publication of BR112012030583A2 publication Critical patent/BR112012030583A2/pt
Publication of BR112012030583B1 publication Critical patent/BR112012030583B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/10—Citrulline; Arginine; Ornithine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485—Exopeptidases (3.4.11-3.4.19)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/11—Aminopeptidases (3.4.11)
    • C12Y304/11006—Aminopeptidase B (3.4.11.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/11—Aminopeptidases (3.4.11)
    • C12Y304/1101—Bacterial leucyl aminopeptidase (3.4.11.10)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/13—Dipeptidases (3.4.13)
    • C12Y304/1302—Beta-Ala-His dipeptidase (3.4.13.20)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

bactéria, e, método para produzir um l-aminoácido - a presente invenção fornece um método para produzir l-aminoácidos, tais como l-arginina, l-citrulina, e l-lisina, usando uma bactéria da família enterobacteriaceare, particularente uma bactéria que pertence ao gênero escherichia ou pantoea, que foi modificada para atenuar a expressão de um ou mais genes selecionadas do grupo que consiste dos genes pepa, pepb, e pepd.
BR112012030583-2A 2010-06-03 2011-06-03 método para produzir um l-aminoácido BR112012030583B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
RU2010122647 2010-06-03
RU2010122647/10A RU2471870C2 (ru) 2010-06-03 2010-06-03 СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА И L-ЦИТРУЛЛИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА pepA
PCT/JP2011/063301 WO2011152565A1 (en) 2010-06-03 2011-06-03 A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family, having attenuated expression of gene(s) encoding peptidase

Publications (2)

Publication Number Publication Date
BR112012030583A2 true BR112012030583A2 (pt) 2015-11-24
BR112012030583B1 BR112012030583B1 (pt) 2020-12-08

Family

ID=44501650

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012030583-2A BR112012030583B1 (pt) 2010-06-03 2011-06-03 método para produzir um l-aminoácido

Country Status (7)

Country Link
US (1) US8722370B2 (pt)
EP (1) EP2576802B1 (pt)
JP (1) JP5846128B2 (pt)
CN (1) CN103003437B (pt)
BR (1) BR112012030583B1 (pt)
RU (1) RU2471870C2 (pt)
WO (1) WO2011152565A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6459962B2 (ja) 2013-10-21 2019-01-30 味の素株式会社 Ｌ−アミノ酸の製造法
RU2013147882A (ru) 2013-10-28 2015-05-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО "АГРИ") СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИЙ СЕМЕЙСТВА Enterobacteriaceae, В КОТОРОЙ НАРУШЕН ПУТЬ ДЕГРАДАЦИИ ПУТРЕСЦИНА
RU2015120052A (ru) * 2015-05-28 2016-12-20 Аджиномото Ко., Инк. Способ получения L-аминокислоты с использованием бактерии семейства Enterobacteriaceae, в которой ослаблена экспрессия гена gshA
US10428359B2 (en) 2016-10-03 2019-10-01 Ajinomoto Co, Inc. Method for producing L-amino acid
CN112266908B (zh) * 2020-10-29 2022-11-08 华东理工大学 一种重组肌肽水解酶突变体及其应用

Family Cites Families (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU875663A1 (ru) 1978-06-30 1982-09-15 Всесоюзный научно-исследовательский институт генетики и селекции промышленных микроорганизмов Штаммы е.coLI ВНИИГенетика VL 334 @ N6 и ВНИИГенетика VL 334 @ N7-продуценты L-треонина и способ их получени
JPS5618596A (en) 1979-07-23 1981-02-21 Ajinomoto Co Inc Production of l-lysine through fermentation process
JPH03501682A (ja) 1988-10-25 1991-04-18 味の素株式会社 Ｌ‐トレオニン生産者としてのエシェリキア・コリｂｋｉｉｍ　ｂ‐３９９６菌株
JPH07108228B2 (ja) 1990-10-15 1995-11-22 味の素株式会社 温度感受性プラスミド
EP0643135B1 (en) 1992-11-10 2001-08-01 Ajinomoto Co., Inc. Dna encoding aspartokinase iii mutants and their use for the production of l-threonine by fermentation
HU221120B1 (en) 1993-10-28 2002-08-28 Ajinomoto Kk Process for producing different substances with use of microorganism
JPH07155184A (ja) 1993-12-08 1995-06-20 Ajinomoto Co Inc 発酵法によるｌ−リジンの製造法
WO1995034672A1 (fr) 1994-06-14 1995-12-21 Ajinomoto Co., Inc. GENE A DESHYDROGENASE α-CETOGLUTARIQUE
HU223706B1 (hu) 1994-12-09 2004-12-28 Ajinomoto Co., Inc. Új lizin dekarboxiláz gén és eljárás L-lizin termelésére
JP2000262288A (ja) 1999-03-16 2000-09-26 Ajinomoto Co Inc コリネ型細菌の温度感受性プラスミド
ATE411378T1 (de) 2000-01-21 2008-10-15 Ajinomoto Kk Verfahren zur herstellung von l-lysin
JP4682454B2 (ja) 2000-06-28 2011-05-11 味の素株式会社 新規変異型ｎ−アセチルグルタミン酸合成酵素及びｌ−アルギニンの製造法
RU2215783C2 (ru) * 2001-05-15 2003-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото - Генетика" МУТАНТНАЯ N-АЦЕТИЛГЛУТАМАТ СИНТАЗА (ВАРИАНТЫ), ФРАГМЕНТ ДНК, ШТАММ БАКТЕРИИ Escherichia coli - ПРОДУЦЕНТ АРГИНИНА (ВАРИАНТЫ) И СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА
JP4380029B2 (ja) 2000-07-05 2009-12-09 味の素株式会社 微生物を利用した物質の製造法
RU2207371C2 (ru) 2000-09-26 2003-06-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-аминокислот семейства l-глутаминовой кислоты, штамм бактерии escherichia coli - продуцент l-аминокислоты (варианты)
RU2208640C2 (ru) 2000-07-06 2003-07-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА, ШТАММ Escherichia coli - ПРОДУЦЕНТ L-АРГИНИНА
CN101597588B (zh) * 2001-02-13 2011-04-06 味之素株式会社 通过埃希氏菌属细菌生产l-氨基酸的方法
US7476531B2 (en) 2001-02-13 2009-01-13 Ajinomoto Co., Inc. Method for producing L-amino acid using bacteria belonging to the genus Escherichia
RU2264459C2 (ru) 2001-08-03 2005-11-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Новая мутантная карбамоилфосфатсинтетаза и способ продукции соединений - производных карбамоилфосфата
RU2229513C2 (ru) 2001-11-23 2004-05-27 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" Способ получения l-аминокислот, штамм escherichia coli - продуцент l-аминокислоты (варианты)
JP4894134B2 (ja) 2003-07-29 2012-03-14 味の素株式会社 物質生産に影響する代謝フラックスの決定方法
PL1664318T3 (pl) 2004-01-30 2010-03-31 Ajinomoto Kk Mikroorganizm wytwarzający L-aminokwas i sposób wytwarzania L-aminokwasu
US8003367B2 (en) 2004-03-16 2011-08-23 Ajinomoto Co., Inc. Method for producing L-amino acids by fermentation using bacteria having enhanced expression of xylose utilization genes
BRPI0516261A (pt) 2004-10-07 2008-08-26 Ajinomoto Kk método para produzir uma substãncia básica por fermentação, e, caldo de fermentação ou produto de fermentação contendo uma substáncia básica
US7915018B2 (en) 2004-10-22 2011-03-29 Ajinomoto Co., Inc. Method for producing L-amino acids using bacteria of the Enterobacteriaceae family
CN101115832A (zh) * 2004-11-26 2008-01-30 协和发酵工业株式会社 工业上有用的微生物
WO2006088235A1 (en) 2005-02-18 2006-08-24 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family
EP1856269B1 (en) 2005-02-18 2008-12-10 Ajinomoto Co., Inc. A METHOD FOR PRODUCING A NON-AROMATIC L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY HAVING EXPRESSION OF THE csrA GENE ATTENUATED
CN101151369B (zh) * 2005-03-29 2017-02-15 协和发酵生化株式会社 二肽的结晶及其制备方法
WO2006123764A1 (en) 2005-05-16 2006-11-23 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the kefb gene
WO2007013639A1 (en) 2005-07-25 2007-02-01 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE cpxR GENE
EP1929027B1 (en) * 2005-08-09 2009-01-14 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE ybiV GENE
JP2007185184A (ja) * 2005-12-16 2007-07-26 Ajinomoto Co Inc Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
EP2186881B1 (en) 2006-03-23 2014-04-23 Ajinomoto Co., Inc. A method for producing an L-amino acid using bacterium of the Enterobacteriaceae family with attenuated expression of a gene coding for small RNA
EP2007873B1 (en) 2006-04-18 2015-11-18 Ajinomoto Co., Inc. A METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE sfmACDFH-fimZ CLUSTER OR THE fimZ GENE
WO2007139219A1 (en) * 2006-06-01 2007-12-06 Ajinomoto Co., Inc. A method for producing an l-amino acid using a bacterium of the enterobacteriaceae family with attenuated expression of the rcsa gene
RU2337961C2 (ru) 2006-07-04 2008-11-10 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) СПОСОБ ПОЛУЧЕНИЯ L-ТРЕОНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ, ПРИНАДЛЕЖАЩЕЙ К РОДУ Escherichia, В КОТОРОЙ ИНАКТИВИРОВАН ОПЕРОН rspAB
RU2006143864A (ru) 2006-12-12 2008-06-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АМИНОКИСЛОТ С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНОВ cynT, cynS, cynX, ИЛИ cynR, ИЛИ ИХ КОМБИНАЦИИ
JP2010041920A (ja) 2006-12-19 2010-02-25 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
JP2010110217A (ja) 2007-02-22 2010-05-20 Ajinomoto Co Inc Ｌ−アミノ酸生産菌及びｌ−アミノ酸の製造法
JP2010130899A (ja) 2007-03-14 2010-06-17 Ajinomoto Co Inc Ｌ−グルタミン酸系アミノ酸生産微生物及びアミノ酸の製造法
JP2010226957A (ja) 2007-10-17 2010-10-14 Ajinomoto Co Inc Ｌ−アミノ酸の製造法
RU2008148283A (ru) 2008-12-09 2010-06-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) (RU) СПОСОБ ПОЛУЧЕНИЯ L-АРГИНИНА С ИСПОЛЬЗОВАНИЕМ БАКТЕРИИ СЕМЕЙСТВА ENTEROBACTERIACEAE, В КОТОРОЙ ОСЛАБЛЕНА ЭКСПРЕССИЯ ГЕНА artI
EP2382320B1 (en) 2009-01-23 2018-04-18 Ajinomoto Co., Inc. Process for producing l-amino acids employing bacteria of the enterobacteriacea family in a culture medium with controlled glycerol concentration
RU2009136544A (ru) 2009-10-05 2011-04-10 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU) Способ получения l-цистеина с использованием бактерии семейства enterobacteriaceae

Also Published As

Publication number Publication date
US20130078682A1 (en) 2013-03-28
BR112012030583B1 (pt) 2020-12-08
JP2013526841A (ja) 2013-06-27
CN103003437B (zh) 2015-05-20
EP2576802B1 (en) 2014-11-12
EP2576802A1 (en) 2013-04-10
CN103003437A (zh) 2013-03-27
WO2011152565A1 (en) 2011-12-08
US8722370B2 (en) 2014-05-13
JP5846128B2 (ja) 2016-01-20
RU2010122647A (ru) 2011-12-10
RU2471870C2 (ru) 2013-01-10

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Legal Events

Date Code Title Description
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 03/06/2011, OBSERVADAS AS CONDICOES LEGAIS.