BR112013025459A2 - análise de alto rendimento de bordas de transgenes - Google Patents

análise de alto rendimento de bordas de transgenes

Info

Publication number
BR112013025459A2
BR112013025459A2 BR112013025459A BR112013025459A BR112013025459A2 BR 112013025459 A2 BR112013025459 A2 BR 112013025459A2 BR 112013025459 A BR112013025459 A BR 112013025459A BR 112013025459 A BR112013025459 A BR 112013025459A BR 112013025459 A2 BR112013025459 A2 BR 112013025459A2
Authority
BR
Brazil
Prior art keywords
transgene
sequences
flanking
edges
analysis
Prior art date
Application number
BR112013025459A
Other languages
English (en)
Inventor
Ning Zhou
Stephen Novak
Zehui Cao
Original Assignee
Dow Agrosciences Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Agrosciences Llc filed Critical Dow Agrosciences Llc
Publication of BR112013025459A2 publication Critical patent/BR112013025459A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

resumo patente de invenção: "análise de alto rendimento de bordas de transgenes". a presente invenção refere-se a um método para identificar sequências desconhecidas de dna que flanqueiam sequências conhecidas de dna. a invenção melhora a exatidão, a sensibilidade e a reprodutibilidade na determinação de sequências desconhecidas de dna que flanqueiam uma sequência conhecida de dna. este método reivindicado pode ser implantado como método de alto rendimento para identificar de modo rápido e eficiente sequências cromossômicas do genoma de plantas que flanqueiam um transgene. a análise posterior destas sequências desconhecidas pode ser utilizada para caracterizar o sítio de inserção do transgene para a identificação de rearranjos, inserções e deleções que resultem da integração do transgene. adicionalmente, a análise das sequências flanqueadoras cromossômicas pode ser utilizada para identificar a localização do transgene no cromossomo.
BR112013025459A 2011-04-05 2012-03-30 análise de alto rendimento de bordas de transgenes BR112013025459A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161471833P 2011-04-05 2011-04-05
PCT/US2012/031334 WO2012138549A1 (en) 2011-04-05 2012-03-30 High through-put analysis of transgene borders

Publications (1)

Publication Number Publication Date
BR112013025459A2 true BR112013025459A2 (pt) 2016-11-29

Family

ID=46966541

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013025459A BR112013025459A2 (pt) 2011-04-05 2012-03-30 análise de alto rendimento de bordas de transgenes

Country Status (14)

Country Link
US (1) US8911943B2 (pt)
EP (1) EP2694682B1 (pt)
JP (1) JP6063443B2 (pt)
KR (1) KR20140024357A (pt)
CN (1) CN103476950B (pt)
AR (1) AR085931A1 (pt)
AU (1) AU2012240460B2 (pt)
BR (1) BR112013025459A2 (pt)
CA (1) CA2829944A1 (pt)
DK (1) DK2694682T3 (pt)
ES (1) ES2650721T3 (pt)
IL (1) IL228527A (pt)
RU (1) RU2603265C2 (pt)
WO (1) WO2012138549A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104334739A (zh) 2012-01-13 2015-02-04 Data生物有限公司 通过新一代测序进行基因分型
KR102269769B1 (ko) 2013-11-04 2021-06-28 코르테바 애그리사이언스 엘엘씨 최적 메이즈 유전자좌
CA2928855A1 (en) 2013-11-04 2015-05-07 Dow Agrosciences Llc Optimal maize loci
JP2017506500A (ja) * 2013-12-10 2017-03-09 コネクシオ ゲノミクス ピーティーワイ リミテッド 遺伝子アリルを同定するための方法及びプローブ
CN105567834A (zh) * 2016-01-29 2016-05-11 中国科学院遗传与发育生物学研究所 一种大米转基因成分的检测方法
CN108034695B (zh) * 2017-12-21 2021-06-25 江苏省农业科学院 一种高效获得t-dna插入侧翼序列的方法及其应用
CN109295163B (zh) * 2018-10-09 2022-11-15 中国农业科学院深圳农业基因组研究所 一种通用长片段染色体步移的方法
US20230193373A1 (en) * 2019-08-20 2023-06-22 Director-General Of National Institute Of Infectious Diseases Method for amplifying nucleotide sequence, and sequence determination
KR20220114958A (ko) 2021-02-09 2022-08-17 주식회사 엘지화학 형질전환 식물체에서 차세대 염기서열 분석을 위한 프로브 세트의 제조 방법

Family Cites Families (14)

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US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
US5986076A (en) 1994-05-11 1999-11-16 Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
RU2087534C1 (ru) * 1995-05-18 1997-08-20 Московский научно-исследовательский институт педиатрии и детской хирургии Рекомбинантная плазмидная днк альфа r 1 - 6, предназначенная для маркирования 13-й и 21-й хромосом человека
US5972693A (en) 1995-10-24 1999-10-26 Curagen Corporation Apparatus for identifying, classifying, or quantifying DNA sequences in a sample without sequencing
US6013486A (en) * 1997-09-17 2000-01-11 Yale University Method for selection of insertion mutants
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Also Published As

Publication number Publication date
AU2012240460B2 (en) 2016-07-07
US8911943B2 (en) 2014-12-16
IL228527A0 (en) 2013-12-31
ES2650721T3 (es) 2018-01-22
RU2013148935A (ru) 2015-05-10
EP2694682B1 (en) 2017-10-25
WO2012138549A1 (en) 2012-10-11
US20120258867A1 (en) 2012-10-11
EP2694682A1 (en) 2014-02-12
AR085931A1 (es) 2013-11-06
DK2694682T3 (da) 2018-01-29
CN103476950A (zh) 2013-12-25
EP2694682A4 (en) 2014-12-31
CA2829944A1 (en) 2012-10-11
JP2014511695A (ja) 2014-05-19
IL228527A (en) 2017-02-28
CN103476950B (zh) 2016-05-11
RU2603265C2 (ru) 2016-11-27
JP6063443B2 (ja) 2017-01-18
AU2012240460A1 (en) 2013-09-12
KR20140024357A (ko) 2014-02-28

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]