BR112013009618A2 - aumento da disponibilidade de nadph para produção de metionina - Google Patents

aumento da disponibilidade de nadph para produção de metionina

Info

Publication number
BR112013009618A2
BR112013009618A2 BR112013009618A BR112013009618A BR112013009618A2 BR 112013009618 A2 BR112013009618 A2 BR 112013009618A2 BR 112013009618 A BR112013009618 A BR 112013009618A BR 112013009618 A BR112013009618 A BR 112013009618A BR 112013009618 A2 BR112013009618 A2 BR 112013009618A2
Authority
BR
Brazil
Prior art keywords
nadph
increased availability
methionine
methionine production
activity
Prior art date
Application number
BR112013009618A
Other languages
English (en)
Inventor
Cédric Boisart
Perrine Vasseur
Rainer Figge
Wanda Dischert
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of BR112013009618A2 publication Critical patent/BR112013009618A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/12—Methionine; Cysteine; Cystine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01001—Pyruvate carboxylase (6.4.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

aumento da disponibilidade de nadph para produção de metionina. a presente invenção se refere a um microorganismo para produção de metionina, onde o dito microorganismo é modificado para melhorar a atividade da transhidrogenase pntab. em um aspecto preferido da invenção, a atividade da transhidrogenase udha é atenuada nos ditos microorganismos. a invenção também se refere a um método para produção de metionina por fermentação.
BR112013009618A 2010-10-25 2011-10-24 aumento da disponibilidade de nadph para produção de metionina BR112013009618A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US40624910P 2010-10-25 2010-10-25
EP10306164 2010-10-25
PCT/EP2011/068499 WO2012055798A1 (en) 2010-10-25 2011-10-24 Increasing nadph availability for methionine production

Publications (1)

Publication Number Publication Date
BR112013009618A2 true BR112013009618A2 (pt) 2016-08-02

Family

ID=43640178

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112013009618A BR112013009618A2 (pt) 2010-10-25 2011-10-24 aumento da disponibilidade de nadph para produção de metionina

Country Status (10)

Country Link
US (1) US9034611B2 (pt)
EP (1) EP2633037B1 (pt)
JP (2) JP2013539987A (pt)
KR (1) KR20140002653A (pt)
CN (2) CN103282488A (pt)
AR (1) AR083468A1 (pt)
BR (1) BR112013009618A2 (pt)
ES (1) ES2637280T3 (pt)
PL (1) PL2633037T3 (pt)
WO (1) WO2012055798A1 (pt)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AR086790A1 (es) 2011-06-29 2014-01-22 Metabolic Explorer Sa Un microorganismo para la produccion de metionina con importacion de glucosa mejorada
CN104411821B (zh) * 2012-06-18 2017-08-08 代谢探索者公司 用于发酵生产甲硫氨酸的重组微生物
US9670479B2 (en) 2013-03-15 2017-06-06 F Cubed, LLC Sample preparation device and methods of use
PL3039153T3 (pl) 2013-08-30 2019-02-28 Evonik Degussa Gmbh Mikroorganizm do wytwarzania metioniny o ulepszonej aktywności syntazy metioniny i wypływie metioniny
CN103710396A (zh) * 2013-12-30 2014-04-09 南京工业大学 一种提高厌氧发酵目标产物转化率的方法
EP3189150A1 (en) * 2014-09-01 2017-07-12 Metabolic Explorer Method and microorganism for methionine production by fermentation with improved methionine efflux
EP3050970B1 (en) 2015-01-28 2019-09-18 Metabolic Explorer Modified microorganism for optimized production of 1,4-butanediol
WO2016162442A1 (en) 2015-04-07 2016-10-13 Metabolic Explorer A modified microorganism for the optimized production of 2,4-dihydroxyburyrate with enhanced 2,4-dihydroxybutyrate efflux
WO2016162712A1 (en) 2015-04-07 2016-10-13 Metabolic Explorer Modified microorganism for the optimized production of 2,4-dihydroxyburyrate
CN108026147B (zh) * 2015-06-04 2021-10-01 Cj第一制糖株式会社 生产o-乙酰-高丝氨酸的微生物和使用其生产o-乙酰-高丝氨酸的方法
PL3331998T3 (pl) 2015-08-07 2020-12-28 Evonik Operations Gmbh Wytwarzanie przez fermentację l-metioniny zależne od tiokarboksylanu białka
EP3347478B1 (en) 2015-09-10 2022-09-21 Metabolic Explorer New lactaldehyde reductases for the production of 1,2-propanediol
US10801050B2 (en) 2015-10-23 2020-10-13 Metabolic Explorer Microorganism modified for the assimilation of levulinic acid
CN108291243A (zh) 2015-11-27 2018-07-17 赢创德固赛有限公司 生产l-甲硫氨酸的方法
WO2018007565A1 (en) 2016-07-08 2018-01-11 Metabolic Explorer Method for the fermentative production of methionine or its hydroxy analog form by microorganisms comprising genes coding sugar phosphotransferase system (pts)
EP3296404A1 (en) 2016-09-15 2018-03-21 Evonik Degussa GmbH Modified microorganism for production of methionine
CN106635945B (zh) * 2016-12-29 2020-05-26 廊坊梅花生物技术开发有限公司 重组菌株及其制备方法和生产l-苏氨酸的方法
EP3342873A1 (en) 2016-12-29 2018-07-04 Metabolic Explorer Conversion of methylglyoxal into hydroxyacetone using enzymes and applications thereof
CN106501201B (zh) * 2016-12-31 2018-06-19 湖南永和阳光生物科技股份有限公司 一种用于检测同型半胱氨酸的酶循环方法
US11162122B2 (en) 2017-07-11 2021-11-02 Adisseo France S.A.S. Methionine-producing yeast
CN108486023B (zh) * 2018-03-08 2020-12-22 江苏省中国科学院植物研究所 一种产生p-羟基肉桂酸的基因工程菌株及其构建方法与应用
EP3788161A1 (en) 2018-05-04 2021-03-10 Ajinomoto Co., Inc. Method for producing l-methionine using a bacterium of the genus pantoea
BR112021005543A2 (pt) 2018-09-28 2021-06-29 Ajinomoto Co., Inc. método para produzir l-metionina
EP3861109A1 (en) 2018-10-05 2021-08-11 Ajinomoto Co., Inc. Method for producing target substance by bacterial fermentation
BR112021014194A2 (pt) 2019-02-22 2021-12-28 Ajinomoto Kk Método para a produção de um l-aminoácido
JP2022526181A (ja) 2019-04-05 2022-05-23 味の素株式会社 Ｌ－アミノ酸の製造方法
JP2022550084A (ja) 2019-09-25 2022-11-30 味の素株式会社 細菌の発酵によるｌ－アミノ酸の製造方法
CN113122487B (zh) * 2020-01-10 2022-05-24 中国科学院微生物研究所 一种高产l-高丝氨酸的重组菌及其制备方法与应用
CN112779200B (zh) * 2021-01-12 2022-07-08 浙江工业大学 高产l-甲硫氨酸的基因工程菌及其构建与应用

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1307477A2 (en) 2000-08-02 2003-05-07 Degussa AG Nucleotide sequences which code for the metf gene
DE10217058A1 (de) 2002-04-17 2003-11-27 Basf Ag Verfahren zur Herstellung von schwefelhaltigen Feinchemikalien
WO2005007862A2 (en) 2003-07-08 2005-01-27 Novus Internation, Inc Methionine recovery processes
FR2862068B1 (fr) * 2003-11-06 2007-10-12 Metabolic Explorer Sa Souches de microorganismes optimisees pour des voies de biosyntheses consommatrices de nadph
DE10359668A1 (de) 2003-12-18 2005-07-14 Basf Ag Verfahren zur Herstellung von Methionin
WO2005111202A1 (en) * 2004-05-12 2005-11-24 Metabolic Explorer Recombinant enzyme with altered feedback sensitivity
US20080286840A1 (en) * 2005-02-07 2008-11-20 Rainer Figge Microorganisms Comprising Enzymes Express with Low Gamma-Elimination Activity
WO2007020295A2 (en) * 2005-08-18 2007-02-22 Basf Ag Microorganisms with increased efficiency for methionine synthesis
CN101356272A (zh) * 2005-08-18 2009-01-28 巴斯夫欧洲公司 甲硫氨酸合成效率增高的微生物
US8389250B2 (en) 2006-01-04 2013-03-05 Metabolic Explorer Methods for producing methionine by culturing a microorganism modified to enhance production of cysteine
BRPI0712219A2 (pt) * 2006-05-24 2012-03-13 Evonik Degussa Gmbh processo para a preparação de l-metionina
WO2009043372A1 (en) * 2007-10-02 2009-04-09 Metabolic Explorer Increasing methionine yield
WO2010020290A1 (en) 2008-08-22 2010-02-25 Metabolic Explorer Producing methionine without n-acetyl methionine
US20100143997A1 (en) * 2008-10-31 2010-06-10 Thomas Buelter Engineered microorganisms capable of producing target compounds under anaerobic conditions
WO2011073738A1 (en) 2009-12-14 2011-06-23 Metabolic Explorer Use of inducible promoters in the production of methionine

Also Published As

Publication number Publication date
AR083468A1 (es) 2013-02-27
EP2633037A1 (en) 2013-09-04
JP2013539987A (ja) 2013-10-31
JP2017169576A (ja) 2017-09-28
US9034611B2 (en) 2015-05-19
CN103282488A (zh) 2013-09-04
ES2637280T3 (es) 2017-10-11
CN106906176A (zh) 2017-06-30
KR20140002653A (ko) 2014-01-08
WO2012055798A1 (en) 2012-05-03
EP2633037B1 (en) 2017-06-28
PL2633037T3 (pl) 2018-02-28
US20130183727A1 (en) 2013-07-18

Similar Documents

Publication Publication Date Title
BR112013009618A2 (pt) aumento da disponibilidade de nadph para produção de metionina
BR112014031425A2 (pt) microrganismo recombinante para produção fermentativa de metionina
PH12014501364A1 (en) Modified microorganisms and methods of making butadiene using same
BR112012024799A2 (pt) método para a produção de l-ornitina por fermentação
BR112013009552A2 (pt) produção de butanol a partir de monóxido de carbono por um micro-organismo recombinante
BRPI0901303A2 (pt) micro-organismo que produz de precursor de l-metionina e método de produção de precursor de l-metionina usando o micro-organismo
EP2318514A4 (en) METHODS, SYSTEMS AND COMPOSITIONS FOR INCREASED MICROORGANISM TOLERANCE AND PREPARATION OF 3-HYDROXYPROPIONIC ACID (3-HP)
ECSP13013114A (es) Extracción de quitina en una sola etapa por hidrólisis enzimática en medio ácido
MX2012007717A (es) Cepas y metodo para la produccion de metionina.
BR112013007632A2 (pt) processo para o tratamento da biomassa para a produção de um produto da biomassa pré tratada
ZA201302088B (en) Process for producing bioethanol by enzymatic hydrolysis of cellulose
BR112013019324A2 (pt) processos para refinamento enzimático de um material celulósico pré-tratado, para hidrolisar e para fermentar um material celulósico pre-tratado, e para produzir um produto de fermentação.
BR112015005416A2 (pt) produção de ácidos orgânicos por meio de fermentação em baixo ph
MX2016002651A (es) Microorganismo modificado para la produccion mejorada de alanina.
EP2669382A4 (en) Cellulosic biomass treatment method, method of producing sugar, alcohol or organic acid from cellulosic biomass
BR112014003546A2 (pt) método para a produção de alcano e micro-organismo recombinante capazes de sintetizar alcano
EP2735612A4 (en) MUTANT BETA GLUCOSIDASE, ENZYME COMPOSITION FOR DECOMPOSITION OF A BIOMASS AND METHOD FOR PRODUCING A SUGAR SOLUTION
BRPI1009574A2 (pt) Método para reduzir a produção de co2 em um processo de fermentação para produzir um álcool, e, microorganismo modificado.
WO2011091156A3 (en) Microbial reduction in a processing stream of a milled product
BR112014018534A8 (pt) método para a fabricação de uma proteína, microrganismo e uso de um microrganismo
PH12015500737A1 (en) Recombinant microorganisms for producing organic acids
BR112013012614A2 (pt) método para despolimerização catalisada por ácido de celulose
EP2507430A4 (en) IMPROVED METHODS FOR TREATING BIOMASS FOR ENZYMATIC HYDROLYSIS
MY164327A (en) Method for producing processed aquatic food and enzyme preparation for modifying property of processed aquatic food
WO2013017289A3 (en) A method for producing biofuel and biogas from insoluble organics

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B25A Requested transfer of rights approved

Owner name: EVONIK DEGUSSA GMBH (DE)

B06T Formal requirements before examination [chapter 6.20 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B25D Requested change of name of applicant approved

Owner name: EVONIK OPERATIONS GMBH (DE)

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL