BR112012017456A2 - "construto, vetor, cianobact´peria, método para produzir alcoóis graxos em uma cianobactéria, biocombustível, e, método de expressão de genes exógenos" - Google Patents

"construto, vetor, cianobact´peria, método para produzir alcoóis graxos em uma cianobactéria, biocombustível, e, método de expressão de genes exógenos"

Info

Publication number
BR112012017456A2
BR112012017456A2 BR112012017456A BR112012017456A BR112012017456A2 BR 112012017456 A2 BR112012017456 A2 BR 112012017456A2 BR 112012017456 A BR112012017456 A BR 112012017456A BR 112012017456 A BR112012017456 A BR 112012017456A BR 112012017456 A2 BR112012017456 A2 BR 112012017456A2
Authority
BR
Brazil
Prior art keywords
construct
cyanobacterium
cyanobacteria
vector
fatty alcohols
Prior art date
Application number
BR112012017456A
Other languages
English (en)
Inventor
Xuefeng Lu
Original Assignee
Shell Internationale Res Maatppij B V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CN201010034404.4A external-priority patent/CN102127563B/zh
Priority claimed from CN201010213758.5A external-priority patent/CN102311966B/zh
Application filed by Shell Internationale Res Maatppij B V filed Critical Shell Internationale Res Maatppij B V
Publication of BR112012017456A2 publication Critical patent/BR112012017456A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Liquid Carbonaceous Fuels (AREA)

Abstract

"construto, vetor, cianobactéria, método para produzir alcoóis graxos em uma cianobactéria, biocombustível, e, método de expressão de genes exógenos" a presente invenção se refere a construtos, vetores e cianobactérias para a síntese de alcoóis graxos, e métodos para produzir alcoóis graxos em cianobactérias. especificamente, a presente revelação se refere a um construto para a síntese de alcoóis graxos em cianobactérias, um vetor que compreende o construto, uma cianobactéria que compreende o construto ou é transformada pelo vetor, e um método para produzir alcoóis graxos em cianobactérias.
BR112012017456A 2010-01-15 2011-01-17 "construto, vetor, cianobact´peria, método para produzir alcoóis graxos em uma cianobactéria, biocombustível, e, método de expressão de genes exógenos" BR112012017456A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CN201010034404.4A CN102127563B (zh) 2010-01-15 2010-01-15 一种集胞藻pcc6803表达外源基因的方法
CN201010213758.5A CN102311966B (zh) 2010-06-30 2010-06-30 用于合成脂肪醇的构建体，载体，蓝细菌，以及在蓝细菌中生产脂肪醇的方法
PCT/EP2011/050555 WO2011086189A2 (en) 2010-01-15 2011-01-17 Constructs, vectors and cyanobacteria for the synthesis of fatty alcohols, and methods for producing fatty alcohols in cyanobacteria

Publications (1)

Publication Number Publication Date
BR112012017456A2 true BR112012017456A2 (pt) 2015-09-15

Family

ID=43859690

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012017456A BR112012017456A2 (pt) 2010-01-15 2011-01-17 "construto, vetor, cianobact´peria, método para produzir alcoóis graxos em uma cianobactéria, biocombustível, e, método de expressão de genes exógenos"

Country Status (4)

Country Link
EP (1) EP2524035A2 (pt)
BR (1) BR112012017456A2 (pt)
CA (1) CA2786244A1 (pt)
WO (1) WO2011086189A2 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013029027A1 (en) * 2011-08-24 2013-02-28 Algenol Biofuels Inc. Separation of productive biomass from non-productive biomass in bioreactors and methods thereof
CN102952818B (zh) 2011-08-26 2016-03-02 中国科学院青岛生物能源与过程研究所 用于在蓝细菌中提高脂肪醇产量的构建体和方法
CN109097378B (zh) * 2018-08-13 2021-08-03 中国科学院青岛生物能源与过程研究所 一种异戊二烯合酶和其编码基因、表达载体、工程菌以及生产异戊二烯的方法及应用
CN111041038B (zh) * 2019-12-02 2022-11-08 天津大学 高效生物合成虾青素的集胞藻6803基因工程菌及构建方法及应用
CN111235172B (zh) * 2020-02-24 2021-10-19 扬州大学 基于lacZ基因和pUC复制子的原核启动子报告系统及其构建方法和应用
CN111549047A (zh) * 2020-04-20 2020-08-18 山东大学 驱动外源基因在蓝藻中高效表达的启动子的筛选方法
CN111560340B (zh) * 2020-04-21 2022-08-23 天津大学 一种生物合成肌醇的集胞藻基因工程菌及构建方法及应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6143538A (en) * 1997-02-20 2000-11-07 The United States Of America As Represented By The United States Department Of Energy Fatty acyl-CoA reductase
CA2650773C (en) * 2006-05-19 2014-12-02 Jay D. Keasling Microorganisms transformed with acetyl-coa carboxylase and wax synthase for production of fatty acid derivatives
CA2678915C (en) * 2007-03-28 2019-04-09 Ls9, Inc. Enhanced production of fatty acid derivatives
EP2464722B1 (en) * 2009-08-11 2017-10-04 Synthetic Genomics, Inc. Microbial production of fatty alcohols

Also Published As

Publication number Publication date
WO2011086189A2 (en) 2011-07-21
WO2011086189A3 (en) 2011-10-13
EP2524035A2 (en) 2012-11-21
CA2786244A1 (en) 2011-07-21

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