BR112012009886A2 - célula, e, método para produzir uma proteína recombinante em uma célula hospedeira - Google Patents
célula, e, método para produzir uma proteína recombinante em uma célula hospedeiraInfo
- Publication number
- BR112012009886A2 BR112012009886A2 BR112012009886A BR112012009886A BR112012009886A2 BR 112012009886 A2 BR112012009886 A2 BR 112012009886A2 BR 112012009886 A BR112012009886 A BR 112012009886A BR 112012009886 A BR112012009886 A BR 112012009886A BR 112012009886 A2 BR112012009886 A2 BR 112012009886A2
- Authority
- BR
- Brazil
- Prior art keywords
- cell
- host cell
- recombinant protein
- producing
- vacuolar
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
- C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Medicinal Chemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US25637909P | 2009-10-30 | 2009-10-30 | |
| US35066810P | 2010-06-02 | 2010-06-02 | |
| PCT/US2010/053903 WO2011053541A1 (en) | 2009-10-30 | 2010-10-25 | Methods for the production of recombinant proteins with improved secretion efficiencies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112012009886A2 true BR112012009886A2 (pt) | 2015-09-15 |
Family
ID=43922476
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112012009886A BR112012009886A2 (pt) | 2009-10-30 | 2010-10-25 | célula, e, método para produzir uma proteína recombinante em uma célula hospedeira |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20130011875A1 (https=) |
| EP (1) | EP2494053A4 (https=) |
| JP (1) | JP2013509180A (https=) |
| KR (1) | KR20140015137A (https=) |
| CN (1) | CN102686731A (https=) |
| AU (1) | AU2010313608A1 (https=) |
| BR (1) | BR112012009886A2 (https=) |
| CA (1) | CA2777487A1 (https=) |
| IN (1) | IN2012DN03823A (https=) |
| MX (1) | MX2012004993A (https=) |
| RU (1) | RU2012122166A (https=) |
| WO (1) | WO2011053541A1 (https=) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2494050A4 (en) * | 2009-10-30 | 2013-10-30 | Merck Sharp & Dohme | GRANULOCYTIC COLONY STIMULATING FACTOR MADE IN GLYCOENGINEERED PICHIA PASTORIS |
| US9102969B2 (en) * | 2011-03-11 | 2015-08-11 | Kaneka Corporation | Method for producing heterologous protein using yeast with disruption of VPS gene |
| DK2800809T3 (en) | 2012-01-05 | 2018-05-22 | Glykos Finland Oy | PROTEASE-DEFECTED FILAMENTARY FUNGAL CELLS AND PROCEDURES FOR USE THEREOF |
| CN102559740B (zh) * | 2012-02-25 | 2013-03-27 | 山东大学 | 一种提高酿酒酵母分泌表达异源蛋白的方法及专用酿酒酵母菌株 |
| WO2013174927A1 (en) | 2012-05-23 | 2013-11-28 | Novartis International Pharmaceutical Limited | Production of fucosylated glycoproteins |
| US20160017020A1 (en) * | 2013-03-06 | 2016-01-21 | Protalix Ltd. | Chimeric polypeptides, polynucleotides encoding same, cells expressing same and methods of producing same |
| US10435731B2 (en) | 2013-07-10 | 2019-10-08 | Glykos Finland Oy | Multiple proteases deficient filamentous fungal cells and methods of use thereof |
| SG11201700446XA (en) | 2014-07-21 | 2017-02-27 | Glykos Finland Oy | Production of glycoproteins with mammalian-like n-glycans in filamentous fungi |
| WO2016079739A2 (en) * | 2014-11-20 | 2016-05-26 | Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. | Compositions and methods for producing polypeptides with a modified glycosylation pattern in plant cells |
| DK3332005T3 (da) * | 2015-08-05 | 2022-01-03 | Lonza Ag | Promotorvarianter |
| EP3568408A4 (en) * | 2017-01-13 | 2020-12-16 | Bolt Threads, Inc. | ELASTOMER PROTEINS |
| US11795487B2 (en) * | 2017-07-31 | 2023-10-24 | Melt & Marble AB | Fungal cell with improved protein production capacity |
| SE2051317A1 (en) * | 2020-11-11 | 2022-05-12 | Nielsen Dina Petranovic | A genetically modified yeast cell |
| CN113604373A (zh) * | 2021-02-08 | 2021-11-05 | 江南大学 | 一种提高人源溶菌酶产量及酶活的毕赤酵母缺陷型菌株 |
| CN113736818A (zh) * | 2021-10-08 | 2021-12-03 | 江南大学 | 一种毕赤酵母中提高人源溶菌酶分泌效率及酶活的方法 |
| CN113862242B (zh) * | 2021-10-15 | 2024-06-25 | 江南大学 | 一种减弱液泡分选提高里氏木霉纤维素酶产量的方法 |
| EP4194560A1 (en) | 2021-12-08 | 2023-06-14 | European Molecular Biology Laboratory | Improved production of secreted proteins in fungal cells |
| CN115851468A (zh) * | 2022-07-18 | 2023-03-28 | 江南大学 | 一种生产人酪蛋白巨肽的重组毕赤酵母及其应用 |
| CN116478905B (zh) * | 2023-02-15 | 2025-07-22 | 北京中医药大学 | 一种高尔基体体外培养方法及其在构建药物毒性筛选系统中的应用 |
| WO2025000334A1 (zh) * | 2023-06-29 | 2025-01-02 | 中国科学院深圳先进技术研究院 | 融合蛋白及其在制备代谢产物中的应用 |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4818700A (en) | 1985-10-25 | 1989-04-04 | Phillips Petroleum Company | Pichia pastoris argininosuccinate lyase gene and uses thereof |
| DK1283265T3 (da) | 2000-05-17 | 2009-11-09 | Mitsubishi Tanabe Pharma Corp | Fremgangsmåde til fremstilling af protein med reduktion af mannosephosphat i sukkerkæden og glycoprotein fremstillet derved |
| US8697394B2 (en) | 2000-06-28 | 2014-04-15 | Glycofi, Inc. | Production of modified glycoproteins having multiple antennary structures |
| US7598055B2 (en) | 2000-06-28 | 2009-10-06 | Glycofi, Inc. | N-acetylglucosaminyltransferase III expression in lower eukaryotes |
| EP2322644A1 (en) | 2000-06-28 | 2011-05-18 | GlycoFi, Inc. | Methods for producing modified glycoproteins |
| US7449308B2 (en) | 2000-06-28 | 2008-11-11 | Glycofi, Inc. | Combinatorial DNA library for producing modified N-glycans in lower eukaryotes |
| US7795002B2 (en) | 2000-06-28 | 2010-09-14 | Glycofi, Inc. | Production of galactosylated glycoproteins in lower eukaryotes |
| US7863020B2 (en) | 2000-06-28 | 2011-01-04 | Glycofi, Inc. | Production of sialylated N-glycans in lower eukaryotes |
| US7625756B2 (en) | 2000-06-28 | 2009-12-01 | GycoFi, Inc. | Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells |
| GB0121088D0 (en) * | 2001-08-31 | 2001-10-24 | Cambridge Consultants | Electric kettle |
| JP4820055B2 (ja) | 2001-12-27 | 2011-11-24 | グライコフィ， インコーポレイテッド | 哺乳動物型糖質構造を操作するための方法 |
| IL165717A0 (en) * | 2002-06-26 | 2006-01-15 | Flanders Interuniversity Inst | A strain of methylotrophic yeast for producing proteins |
| AU2003291448A1 (en) | 2002-11-12 | 2004-06-03 | Purdue Research Foundation | Benzoate inducible promoters |
| EP2341128A1 (en) | 2003-12-24 | 2011-07-06 | GlycoFi, Inc. | Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins |
| US7479389B2 (en) | 2004-03-02 | 2009-01-20 | Glycofi, Inc. | ARG1, ARG2, ARG3, HIS1, HIS2, HIS5, HIS6 genes and methods for stable genetic integration |
| EP1730293A2 (en) | 2004-03-17 | 2006-12-13 | Glycofi, Inc. | Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast |
| US7465577B2 (en) | 2004-04-29 | 2008-12-16 | Glycofi, Inc. | Methods for reducing or eliminating α-mannosidase resistant glycans for the production of glycoproteins |
| CA2606253A1 (en) | 2005-04-21 | 2006-10-26 | Uti Limited Partnership | Pcr for mrsa sccmec typing |
| CA2651456A1 (en) | 2006-05-19 | 2007-11-29 | Glycofi, Inc. | Recombinant vectors |
| MX2008014744A (es) * | 2006-05-19 | 2009-02-10 | Glycofi Inc | Composiciones de eritrocpoyetina. |
-
2010
- 2010-10-25 EP EP20100827360 patent/EP2494053A4/en not_active Withdrawn
- 2010-10-25 BR BR112012009886A patent/BR112012009886A2/pt not_active IP Right Cessation
- 2010-10-25 US US13/503,707 patent/US20130011875A1/en not_active Abandoned
- 2010-10-25 RU RU2012122166/10A patent/RU2012122166A/ru not_active Application Discontinuation
- 2010-10-25 JP JP2012536916A patent/JP2013509180A/ja not_active Withdrawn
- 2010-10-25 IN IN3823DEN2012 patent/IN2012DN03823A/en unknown
- 2010-10-25 KR KR1020127010917A patent/KR20140015137A/ko not_active Withdrawn
- 2010-10-25 AU AU2010313608A patent/AU2010313608A1/en not_active Abandoned
- 2010-10-25 MX MX2012004993A patent/MX2012004993A/es not_active Application Discontinuation
- 2010-10-25 WO PCT/US2010/053903 patent/WO2011053541A1/en not_active Ceased
- 2010-10-25 CN CN2010800519511A patent/CN102686731A/zh active Pending
- 2010-10-25 CA CA2777487A patent/CA2777487A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20130011875A1 (en) | 2013-01-10 |
| CA2777487A1 (en) | 2011-05-05 |
| CN102686731A (zh) | 2012-09-19 |
| IN2012DN03823A (https=) | 2015-08-28 |
| EP2494053A1 (en) | 2012-09-05 |
| AU2010313608A1 (en) | 2012-06-07 |
| KR20140015137A (ko) | 2014-02-06 |
| WO2011053541A1 (en) | 2011-05-05 |
| JP2013509180A (ja) | 2013-03-14 |
| MX2012004993A (es) | 2012-06-12 |
| RU2012122166A (ru) | 2013-12-10 |
| EP2494053A4 (en) | 2013-10-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE AS 4A E 5A ANUIDADES. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2344 DE 08-12-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |
|
| B25B | Requested transfer of rights rejected |
Owner name: MERCK SHARP AND DOHME CORP. (US) |
|
| B25E | Requested change of name of applicant rejected |
Owner name: MERCK SHARP AND DOHME CORP. (US) |
|
| B25H | Request for change of headquarter rejected |
Owner name: MERCK SHARP AND DOHME CORP. (US) |