BR112021010338A2 - Células hospedeiras fúngicas filamentosas modificadas - Google Patents

Células hospedeiras fúngicas filamentosas modificadas

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Publication number
BR112021010338A2
BR112021010338A2 BR112021010338A BR112021010338A BR112021010338A2 BR 112021010338 A2 BR112021010338 A2 BR 112021010338A2 BR 112021010338 A BR112021010338 A BR 112021010338A BR 112021010338 A BR112021010338 A BR 112021010338A BR 112021010338 A2 BR112021010338 A2 BR 112021010338A2
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BR
Brazil
Prior art keywords
filamentous fungal
fungal host
cells
host cells
modified
Prior art date
Application number
BR112021010338A
Other languages
English (en)
Inventor
Chiho Inoue
Michael Rey
Nicholas Jochumsen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of BR112021010338A2 publication Critical patent/BR112021010338A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/685—Aspergillus niger
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

células hospedeiras fúngicas filamentosas modificadas. a presente invenção refere-se à célula hospedeira fúngica filamentosa mutada que produz um polipeptídeo secretado de interesse, em que uma esteroide desidrogenase putativa nativa é modificada, truncada, parcial ou completamente inativada, está presente em um nível reduzido ou é eliminada em comparação a uma célula progenitora não mutada, e em que a dita esteroide desidrogenase putativa nativa compreende pelo menos um motivo de aminoácidos conservado selecionado dentre: ygar e/ou vphs[w/y]f e/ou qc[a/v/s]rrl e/ou lkkytlp e/ou cphyt, e a métodos para produzir um polipeptídeo secretado de interesse nas ditas células, assim como métodos para produzir as ditas células.
BR112021010338A 2018-11-28 2019-11-26 Células hospedeiras fúngicas filamentosas modificadas BR112021010338A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862772301P 2018-11-28 2018-11-28
PCT/US2019/063415 WO2020112881A1 (en) 2018-11-28 2019-11-26 Modified filamentous fungal host cells

Publications (1)

Publication Number Publication Date
BR112021010338A2 true BR112021010338A2 (pt) 2021-11-16

Family

ID=69005864

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021010338A BR112021010338A2 (pt) 2018-11-28 2019-11-26 Células hospedeiras fúngicas filamentosas modificadas

Country Status (7)

Country Link
US (1) US20220025423A1 (pt)
EP (1) EP3887524A1 (pt)
JP (1) JP2022513649A (pt)
CN (1) CN113302303A (pt)
BR (1) BR112021010338A2 (pt)
CA (1) CA3121271A1 (pt)
WO (1) WO2020112881A1 (pt)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109182145B (zh) * 2018-09-29 2021-05-11 武汉友芹种苗技术有限公司 一种棘孢曲霉菌株及其应用
CN114561296B (zh) * 2021-12-09 2023-09-22 杨凌未来中科环保科技有限公司 一种棘孢曲霉菌及其应用
CN115838742A (zh) * 2022-10-18 2023-03-24 华中农业大学 南方根结线虫去甲基化酶Mi-NMAD-1/2基因及其应用

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DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
DK0765394T3 (da) 1994-06-03 2001-12-10 Novozymes As Oprensede Myceliopthora-laccaser og nukleinsyrer der koder for disse
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US6506559B1 (en) 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
AU6188599A (en) 1998-10-26 2000-05-15 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
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AR050895A1 (es) 2004-10-04 2006-11-29 Novozymes As Polipeptidos que tienen actividad de fitasa y polinucleotidos que los codifican
WO2010039840A1 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for producing polypeptides in enzyme-deficient mutants of fusarium venenatum
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WO2015144936A1 (en) 2014-03-28 2015-10-01 Novozymes A/S Resolubilization of protein crystals at low ph
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WO2018167153A1 (en) 2017-03-17 2018-09-20 Novozymes A/S Improved filamentous fungal host cell
WO2019046703A1 (en) 2017-09-01 2019-03-07 Novozymes A/S METHODS OF ENHANCING GENOME EDITION IN FUNGI

Also Published As

Publication number Publication date
US20220025423A1 (en) 2022-01-27
CN113302303A (zh) 2021-08-24
JP2022513649A (ja) 2022-02-09
WO2020112881A1 (en) 2020-06-04
EP3887524A1 (en) 2021-10-06
CA3121271A1 (en) 2020-06-04

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