BR112012008053B8 - Método de produção de heparosano substancialmente puro a partir de e. coli k5 - Google Patents

Método de produção de heparosano substancialmente puro a partir de e. coli k5

Info

Publication number
BR112012008053B8
BR112012008053B8 BR112012008053A BR112012008053A BR112012008053B8 BR 112012008053 B8 BR112012008053 B8 BR 112012008053B8 BR 112012008053 A BR112012008053 A BR 112012008053A BR 112012008053 A BR112012008053 A BR 112012008053A BR 112012008053 B8 BR112012008053 B8 BR 112012008053B8
Authority
BR
Brazil
Prior art keywords
coli
heparosan
substantially pure
producing substantially
pure heparosan
Prior art date
Application number
BR112012008053A
Other languages
English (en)
Other versions
BR112012008053B1 (pt
BR112012008053A2 (pt
Inventor
S Dordick Jonathan
J Linhardt Robert
Ujjwal Bhaskar
Zhenyu Wang
Original Assignee
Rensselaer Polytech Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43649920&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112012008053(B8) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Rensselaer Polytech Inst filed Critical Rensselaer Polytech Inst
Publication of BR112012008053A2 publication Critical patent/BR112012008053A2/pt
Publication of BR112012008053B1 publication Critical patent/BR112012008053B1/pt
Publication of BR112012008053B8 publication Critical patent/BR112012008053B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/006—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence; Gellans; Succinoglycans; Arabinogalactans; Tragacanth or gum tragacanth or traganth from Astragalus; Gum Karaya from Sterculia urens; Gum Ghatti from Anogeissus latifolia; Derivatives thereof
    • C08B37/0063—Glycosaminoglycans or mucopolysaccharides, e.g. keratan sulfate; Derivatives thereof, e.g. fucoidan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Materials Engineering (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Sustainable Development (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)

Abstract

fermentação e purificação de heparosano k5. um método para a produção de heparosano da cultura de fermentação de e. coli k5 adequado para a produção industrial, exibindo rendimento e pureza superiores, volumes de cultura menores, crescimento mais rápido e custos mais baixos.
BR112012008053A 2009-09-01 2010-08-30 Método de produção de heparosano substancialmente puro a partir de e. coli k5 BR112012008053B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US27567509P 2009-09-01 2009-09-01
US61/275,675 2009-09-01
PCT/US2010/047183 WO2011028668A2 (en) 2009-09-01 2010-08-30 K5 heparosan fermentation and purification

Publications (3)

Publication Number Publication Date
BR112012008053A2 BR112012008053A2 (pt) 2017-06-13
BR112012008053B1 BR112012008053B1 (pt) 2021-04-20
BR112012008053B8 true BR112012008053B8 (pt) 2022-02-15

Family

ID=43649920

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012008053A BR112012008053B8 (pt) 2009-09-01 2010-08-30 Método de produção de heparosano substancialmente puro a partir de e. coli k5

Country Status (11)

Country Link
US (1) US8883452B2 (pt)
EP (1) EP2473614A4 (pt)
JP (1) JP5830464B2 (pt)
KR (1) KR101515892B1 (pt)
CN (1) CN102712942B (pt)
BR (1) BR112012008053B8 (pt)
MY (1) MY163367A (pt)
RU (1) RU2564566C2 (pt)
SG (1) SG178349A1 (pt)
TW (1) TWI485251B (pt)
WO (1) WO2011028668A2 (pt)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2898515C (en) * 2013-01-25 2022-01-04 Marinova Pty Ltd Methods for depyrogentating a seaweed extract
CN103149198B (zh) * 2013-02-08 2015-04-22 浙江工业大学 一种酶法快速定量检测heparosan的方法
EP3620525A1 (en) 2013-10-02 2020-03-11 Ajinomoto Co., Inc. Heparosan-producing bacterium and heparosan manufacturing method
CN105602980B (zh) * 2016-01-25 2019-02-01 浙江工业大学 一种waaR基因缺陷型细菌合成低分子量肝素前体的方法
CN108624516B (zh) * 2017-03-20 2022-08-26 华东理工大学 一种提高发酵细胞中的代谢产物量及制备idms标准品的方法
WO2021199444A1 (en) * 2020-04-03 2021-10-07 Rensselaer Polytechnic Institute Method for producing heparosan and bacterium of genus escherichia having heparosan-producing ability
EP4067500A1 (en) * 2021-04-01 2022-10-05 Givaudan SA Heparosan-producing recombinant cells
CN112791186A (zh) * 2021-04-14 2021-05-14 江苏艾洛特医药研究院有限公司 一种多糖纳米复合材料及其制备与应用

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2669932B1 (fr) * 1990-12-03 1994-07-01 Sanofi Sa Nouvel heparosane-n,o-sulfate, son procede de preparation et les compositions pharmaceutiques qui le contiennent.
FR2684385B1 (fr) * 1991-11-28 1997-08-01 Sanofi Elf Heparosanes-n,o-sulfates de haute masse moleculaire, leur procede de preparation et les compositions pharmaceutiques qui les contiennent.
US20060188966A1 (en) * 1998-04-02 2006-08-24 Deangelis Paul L Natural, chimeric and hybrid glycosaminoglycan polymers and methods of making and using same
ITMI991465A1 (it) * 1999-07-02 2001-01-02 Inalco Spa Processo per la preparazione dei polisaccaridi k4 e k5 da escherichiacoli
JP4084099B2 (ja) * 2002-06-20 2008-04-30 生化学工業株式会社 Ｎ−アセチルヘパロサン画分の製造方法
AU2003293122A1 (en) * 2002-11-27 2004-06-23 Massachusetts Institute Of Technology Methods for synthesizing polysaccharides
ITMI20031618A1 (it) * 2003-08-06 2005-02-07 Inalco Spa Derivati polisaccaridici dotati di alta attivita'
JP4505631B2 (ja) * 2004-03-31 2010-07-21 独立行政法人産業技術総合研究所 ヘパラン硫酸糖鎖を付加したヘパリン結合性タンパク質、その製造方法及びそれを含有する医薬組成物
JP2005290383A (ja) * 2004-04-05 2005-10-20 Seikagaku Kogyo Co Ltd ６−ｏ−硫酸化ｎ−アセチルヘパロサン及び造血幹細胞増殖助剤
FR2874931B1 (fr) * 2004-09-08 2006-11-24 Aventis Pharma Sa Procede de production de polysaccharide k5
JP2007252396A (ja) * 2006-03-20 2007-10-04 Kitasato Gakuen 医療用透析液の製造装置および製造方法
EP2173360B1 (en) 2007-03-30 2014-11-19 The Board of Regents for the University of Oklahoma Heparosan-based biomaterials and coatings and methods of production and use thereof
DE102007016707A1 (de) * 2007-04-04 2008-10-09 Qiagen Gmbh Verfahren zur Aufreinigung von Biomolekülen

Also Published As

Publication number Publication date
JP2013503606A (ja) 2013-02-04
RU2564566C2 (ru) 2015-10-10
EP2473614A4 (en) 2013-04-24
EP2473614A2 (en) 2012-07-11
BR112012008053B1 (pt) 2021-04-20
BR112012008053A2 (pt) 2017-06-13
US20120157669A1 (en) 2012-06-21
KR20120090948A (ko) 2012-08-17
WO2011028668A4 (en) 2011-09-09
RU2012105621A (ru) 2013-10-10
CN102712942A (zh) 2012-10-03
JP5830464B2 (ja) 2015-12-09
WO2011028668A2 (en) 2011-03-10
TWI485251B (zh) 2015-05-21
TW201114900A (en) 2011-05-01
US8883452B2 (en) 2014-11-11
CN102712942B (zh) 2014-05-14
WO2011028668A3 (en) 2011-07-28
KR101515892B1 (ko) 2015-05-04
MY163367A (en) 2017-09-15
SG178349A1 (en) 2012-03-29

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B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

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B16C Correction of notification of the grant [chapter 16.3 patent gazette]

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