BR0308424A - Métodos e meios para infra-regular eficientemente a expressão de qualquer gene de interesse em células e organismos eucarióticos - Google Patents

Métodos e meios para infra-regular eficientemente a expressão de qualquer gene de interesse em células e organismos eucarióticos

Info

Publication number
BR0308424A
BR0308424A BR0308424-8A BR0308424A BR0308424A BR 0308424 A BR0308424 A BR 0308424A BR 0308424 A BR0308424 A BR 0308424A BR 0308424 A BR0308424 A BR 0308424A
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BR
Brazil
Prior art keywords
organisms
interest
expression
methods
gene
Prior art date
Application number
BR0308424-8A
Other languages
English (en)
Portuguese (pt)
Inventor
Ming-Bo Wang
Peter Waterhouse
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Commw Scient Ind Res Org
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Publication date
Application filed by Commw Scient Ind Res Org filed Critical Commw Scient Ind Res Org
Publication of BR0308424A publication Critical patent/BR0308424A/pt

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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8249Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111General methods applicable to biologically active non-coding nucleic acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/11Antisense
    • C12N2310/111Antisense spanning the whole gene, or a large part of it
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/10Type of nucleic acid
    • C12N2310/14Type of nucleic acid interfering nucleic acids [NA]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00Production
    • C12N2330/30Production chemically synthesised

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
BR0308424-8A 2002-03-14 2003-03-12 Métodos e meios para infra-regular eficientemente a expressão de qualquer gene de interesse em células e organismos eucarióticos BR0308424A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36385102P 2002-03-14 2002-03-14
PCT/AU2003/000292 WO2003076619A1 (en) 2002-03-14 2003-03-12 Modified gene-silencing rna and uses thereof

Publications (1)

Publication Number Publication Date
BR0308424A true BR0308424A (pt) 2005-02-22

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BR0308424-8A BR0308424A (pt) 2002-03-14 2003-03-12 Métodos e meios para infra-regular eficientemente a expressão de qualquer gene de interesse em células e organismos eucarióticos

Country Status (9)

Country Link
US (2) US7576262B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP (1) EP1487980A4 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JP2005519607A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CN (1) CN1646687A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
AU (1) AU2003209814B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
BR (1) BR0308424A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
CA (1) CA2478904A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IN (1) IN2004KO01268A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO2003076619A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Families Citing this family (197)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AUPP249298A0 (en) 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
KR20010042069A (ko) 1998-03-20 2001-05-25 베니텍 오스트레일리아 리미티드 유전자 발현 조절방법
CN1375004A (zh) * 1999-04-21 2002-10-16 惠氏公司 抑制多核苷酸序列的功能的方法和组合物
US7485715B2 (en) * 1999-06-18 2009-02-03 Ceres, Inc. Sequence-determined DNA encoding AP2 domain polypeptides
US7479555B2 (en) * 1999-07-21 2009-01-20 Ceres, Inc. Polynucleotides having a nucleotide sequence that encodes a polypeptide having MOV34 family activity
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
US7612194B2 (en) 2001-07-24 2009-11-03 Monsanto Technology Llc Nucleic acid sequences from Diabrotica virgifera virgifera LeConte and uses thereof
EP1478656B1 (en) 2002-02-01 2009-09-16 Life Technologies Corporation Oligonucleotide compositions with enhanced efficiency
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
US20030166282A1 (en) 2002-02-01 2003-09-04 David Brown High potency siRNAS for reducing the expression of target genes
WO2005017157A1 (en) 2003-08-15 2005-02-24 Commonwealth Scientific And Industrial Research Organisation (Csiro) Methods and means for altering fiber characteristics in fiber-producing plants
EP1664298A4 (en) * 2003-09-12 2010-03-17 Commw Scient Ind Res Org MODIFIED GEN-SILENCING NUCLEIC ACID MOLECULES AND USES THEREOF
ATE526407T1 (de) * 2003-11-17 2011-10-15 Commw Scient Ind Res Org Insektenresistenz durch inhibierung von genexpression
US20060235212A1 (en) * 2004-12-22 2006-10-19 Nickolai Alexandrov Nucleic acid sequences encoding zinc finger proteins
US8049069B2 (en) 2004-03-31 2011-11-01 Commonwealth Scientific And Industrial Research Organisation Genes involved in plant fibre development
CA2762011C (en) 2004-04-09 2019-05-07 Monsanto Technology Llc Compositions and methods for control of insect infestations in plants
US7642072B2 (en) * 2004-07-26 2010-01-05 Enzon Pharmaceuticals, Inc. Optimized interferon-beta gene
BRPI0514342A2 (pt) 2004-08-13 2009-10-06 Basf Plant Science Gmbh molécula de rna de filamento duplo, agrupamento de moléculas de rna de filamento duplo, planta transgênica, e, métodos para controlar a infecção de uma planta por um nematóide parasìtico e para fabricar uma planta transgênica
US20060247197A1 (en) 2004-10-04 2006-11-02 Van De Craen Marc Method for down-regulating gene expression in fungi
WO2006070227A2 (en) * 2004-10-04 2006-07-06 Devgen Nv Method for down-regulating gene expression in fungi
US7429692B2 (en) * 2004-10-14 2008-09-30 Ceres, Inc. Sucrose synthase 3 promoter from rice and uses thereof
CN101107362A (zh) 2004-10-21 2008-01-16 文甘扎公司 用于赋予对植物病害生物和植物病原体抗性的方法和材料
US8053636B2 (en) 2004-10-29 2011-11-08 Bayer Bioscience N.V. Stress tolerant cotton plants
US9758790B2 (en) 2004-12-08 2017-09-12 Ceres, Inc. Modulating the level of components within plants
WO2007087601A2 (en) * 2006-01-25 2007-08-02 Ceres, Inc. Modulating plant protein levels
WO2008069878A2 (en) 2006-10-27 2008-06-12 Ceres, Inc. Modulating lignin in plants
US7335760B2 (en) * 2004-12-22 2008-02-26 Ceres, Inc. Nucleic acid sequences encoding zinc finger proteins
CA2598436A1 (en) * 2005-02-22 2006-08-31 Ceres, Inc. Modulating plant alkaloids
ATE530656T1 (de) 2005-02-23 2011-11-15 Univ North Carolina State Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene.
US20060236421A1 (en) * 2005-04-14 2006-10-19 Pennell Roger I Secondary metabolite production via manipulation of genome methylation
WO2006115575A1 (en) * 2005-04-20 2006-11-02 Ceres Inc. Regulatory regions from papaveraceae
AU2006249522A1 (en) 2005-05-25 2006-11-30 Pioneer Hi-Bred International, Inc. Methods for improving crop plant architecture and yield
US8124839B2 (en) * 2005-06-08 2012-02-28 Ceres, Inc. Identification of terpenoid-biosynthesis related regulatory protein-regulatory region associations
AP2008004416A0 (en) 2005-09-16 2008-04-30 Monsanto Technology Llc Methods for genetic control of insect infestationsin plants and compositions thereof
US20100062137A1 (en) * 2005-09-30 2010-03-11 Steven Craig Bobzin Modulating plant tocopherol levels
US20090178160A1 (en) * 2005-10-25 2009-07-09 Joon-Hyun Park Modulation of Triterpenoid Content in Plants
CN101356279A (zh) 2005-11-10 2009-01-28 先锋高级育种国际公司 Dof(具有一指的dna结合)序列和使用方法
US8222482B2 (en) * 2006-01-26 2012-07-17 Ceres, Inc. Modulating plant oil levels
CN101421295B (zh) 2006-02-09 2012-05-02 先锋高级育种国际公司 用于提高作物植物中的氮利用效率的基因
US20070199090A1 (en) * 2006-02-22 2007-08-23 Nestor Apuya Modulating alkaloid biosynthesis
WO2007120989A2 (en) * 2006-02-24 2007-10-25 Ceres, Inc. Shade regulatory regions
WO2007117693A2 (en) * 2006-04-07 2007-10-18 Ceres, Inc. Regulatory protein-regulatory region associations related to alkaloid biosynthesis
AR060523A1 (es) 2006-04-19 2008-06-25 Pioneer Hi Bred Int Moleculas de polinucleotidos aislados que corresponden a alelos mutantes y tipo salvaje del gen de maiz d9 y metodos para usarlas
US20100024070A1 (en) * 2006-05-15 2010-01-28 Steven Craig Bobzin Modulation of oil levels in plants
WO2007147068A2 (en) * 2006-06-14 2007-12-21 Ceres, Inc. Increasing uv-b tolerance in plants
US20130191941A1 (en) 2006-07-05 2013-07-25 Shing Kwok Modulating light response pathways in plants, increasing light-related tolerances in plants, and increasing biomass in plants
US8222388B2 (en) 2006-11-22 2012-07-17 Ceres, Inc. Broadly expressing regulatory regions
US20100151109A1 (en) * 2006-12-15 2010-06-17 Amr Saad Ragab Modulation of plant protein levels
CN101578371B (zh) 2007-01-11 2014-04-16 拜尔作物科学公司 亚基因组特异性等位基因在棉花中的差异表达及其用途
WO2008095886A1 (en) 2007-02-06 2008-08-14 Basf Plant Science Gmbh Compositions and methods using rna interference for control of nematodes
BRPI0809130A8 (pt) * 2007-03-21 2021-11-03 Brookhaven Science Ass Llc Composições combinadas hairpin-antissenso e métodos para modulação da expressão
AU2008257572A1 (en) 2007-05-25 2008-12-04 Cropdesign N.V. Yield enhancement in plants by modulation of maize Alfins
US20110131668A1 (en) 2007-08-14 2011-06-02 Commonwealth Scientific And Industrial Research Organisation Improved gene silencing methods
US8097712B2 (en) * 2007-11-07 2012-01-17 Beelogics Inc. Compositions for conferring tolerance to viral disease in social insects, and the use thereof
MX2010005565A (es) 2007-11-20 2010-06-11 Pioneer Hi Bred Int Genes de señalizacion de etileno en maiz y su modulacion para mejor tolerancia al estres en plantas.
JP5822466B2 (ja) 2008-03-04 2015-11-24 ワシントン ステイト ユニバーシティー 種子油および膜脂質における脂肪酸不飽和化の示差的調節のための組成物および方法
CN104969864A (zh) 2008-05-26 2015-10-14 拜尔作物科学公司 改变产纤维植物中纤维强度的方法和手段
CN102202498B (zh) 2008-07-21 2016-09-07 澳大利亚联邦科学与工业研究组织 改良的棉籽油及应用
US8419145B2 (en) * 2008-07-25 2013-04-16 Eastman Kodak Company Inkjet printhead and method of printing with multiple drop volumes
US8298794B2 (en) * 2008-10-09 2012-10-30 Ceres, Inc. Cinnamyl-alcohol dehydrogenases
EP2344640A1 (en) 2008-10-30 2011-07-20 Pioneer Hi-Bred International Inc. Manipulation of glutamine synthetases (gs) to improve nitrogen use efficiency and grain yield in higher plants
CA2743707A1 (en) 2008-12-04 2010-06-10 Pioneer Hi-Bred International, Inc. Methods and compositions for enhanced yield by targeted expression of knotted1
US8309530B2 (en) 2009-02-04 2012-11-13 Washington State University Compositions and methods for modulating ghrelin-mediated conditions
US8716553B2 (en) 2009-03-02 2014-05-06 Pioneer Hi Bred International Inc NAC transcriptional activators involved in abiotic stress tolerance
CA2754956A1 (en) 2009-03-20 2010-09-23 Basf Plant Science Company Gmbh Nematode-resistant transgenic plants
US20100257634A1 (en) * 2009-04-03 2010-10-07 Venganza Inc. Bioassay for gene silencing constructs
MX2011010845A (es) 2009-04-14 2011-11-02 Pioneer Hi Bred Int La modulacion de acc sintasa mejora el rendimiento de las plantas en condiciones de bajo contenido de nitrogeno.
CN102439032B (zh) 2009-05-04 2015-07-22 先锋国际良种公司 通过调节ap2转录因子增加植物中的产量
WO2010128465A1 (en) 2009-05-05 2010-11-11 Beeologics, Llc Prevention and treatment of nosema disease in bees
EP2325330A4 (en) * 2009-06-02 2012-02-22 Inc Admin Agency Naro METHOD FOR SIMULTANEOUS DETECTION OF PSTVd AND TCDVd VIROIDS
CA2765287C (en) 2009-06-15 2018-12-11 Bayer Bioscience N.V. Nicotiana benthamiana plants deficient in xylosyltransferase activity
CN102573451A (zh) 2009-07-20 2012-07-11 希尔雷斯股份有限公司 具有增加的生物质的转基因植物
MX2012001080A (es) 2009-07-24 2012-03-26 Pionner Hi Bred International Inc Uso de agrupaciones de componentes del dominio de dimerizacion para modular la arquitectura de las plantas.
EP2483404A1 (en) 2009-10-02 2012-08-08 Pioneer Hi-Bred International Inc. Down-regulation of acc synthase for improved plant performance
US8962584B2 (en) 2009-10-14 2015-02-24 Yissum Research Development Company Of The Hebrew University Of Jerusalem, Ltd. Compositions for controlling Varroa mites in bees
WO2011069953A1 (en) 2009-12-09 2011-06-16 Basf Plant Science Company Gmbh Methods for increasing the resistance of plants to fungi by silencing the fungal smt1-gene
CN102906267A (zh) 2010-01-06 2013-01-30 先锋国际良种公司 玉米光合作用组织和非光合作用组织昼夜节律的鉴定及其在改良作物方面的用途
EP2539362A1 (en) 2010-02-23 2013-01-02 BASF Plant Science Company GmbH Nematode-resistant transgenic plants
MX2012010479A (es) 2010-03-08 2012-10-09 Monsanto Technology Llc Moleculas polinucleotidicas para regulacion genetica en plantas.
CA3130705A1 (en) 2010-04-15 2011-10-20 National Research Council Of Canada Genes and proteins for aromatic polyketide synthesis
EA201291178A1 (ru) 2010-05-06 2013-04-30 Пайонир Хай-Бред Интернэшнл, Инк. Ген и белок acc-синтазы 3 маиса и их применения
US9441233B2 (en) 2010-05-06 2016-09-13 Ceres, Inc. Transgenic plants having increased biomass
WO2012004013A2 (en) 2010-07-08 2012-01-12 Bayer Bioscience N.V. Glucosinolate transporter protein and uses thereof
EP2593468B1 (en) 2010-07-12 2020-06-10 The State of Israel, Ministry of Agriculture and Rural Development, Agricultural Research Organization, (A.R.O.), Volcani Center Isolated polynucleotides and methods and plants using same for regulating plant acidity
US20120107355A1 (en) 2010-10-27 2012-05-03 Harrisvaccines, Inc. Method of rapidly producing improved vaccines for animals
US8822427B2 (en) 2010-10-27 2014-09-02 Harrisvaccines Methods and compositions to protect aquatic invertebrates from disease
BR112013010278B1 (pt) 2010-10-27 2020-12-29 Ceres, Inc método para produzir uma planta, método para modular a composição de biomassa em uma planta, ácido nucleico isolado e método para alterar a composição de biomassa em uma planta
US10004797B2 (en) 2010-10-27 2018-06-26 Harrisvaccines, Inc. Method of rapidly producing improved vaccines for animals
CA2822720C (en) 2010-12-24 2020-05-12 Bayer Cropscience Nv Brassica plant comprising a mutant alcatraz allele
BR112013018531A2 (pt) 2011-01-24 2016-10-18 Bayer Cropscience Nv uso do promotor rd29 ou fragmentos do mesmo para expressão induzível por estresse de transgenes em algodão
AR085533A1 (es) 2011-03-23 2013-10-09 Pioneer Hi Bred Int Metodos de produccion de un locus de rasgos transgenicos complejo
EP2697377A1 (en) 2011-04-15 2014-02-19 Pioneer Hi-Bred International Inc. Self-reproducing hybrid plants
CN103502269A (zh) 2011-04-29 2014-01-08 先锋国际良种公司 下调同源域-亮氨酸拉链i类同源盒基因以改进植物性能
US9631196B2 (en) 2011-06-10 2017-04-25 Temasek Life Sciences Laboratory Limited Genetic manipulation and expression systems for Pucciniomycotina and Ustilaginomycotina subphyla
US20140216118A1 (en) 2011-06-14 2014-08-07 Synthon Biopharmaceuticals B.V. Compositions and Methods for Making and Biocontaining Auxotrophic Transgenic Plants
CA2844470A1 (en) 2011-06-21 2013-05-10 Pioneer Hi-Bred International, Inc. Methods and compositions for producing male sterile plants
US9181532B2 (en) 2011-07-29 2015-11-10 Icon Genetics Gmbh Production of galactosylated N-glycans in plants
US20140215655A1 (en) 2011-08-12 2014-07-31 Bayer Cropscience Nv Guard cell-specific expression of transgenes in cotton
US10829828B2 (en) 2011-09-13 2020-11-10 Monsanto Technology Llc Methods and compositions for weed control
US9840715B1 (en) 2011-09-13 2017-12-12 Monsanto Technology Llc Methods and compositions for delaying senescence and improving disease tolerance and yield in plants
MX343072B (es) 2011-09-13 2016-10-21 Monsanto Technology Llc Metodos y composiciones para controlar malezas.
CA2848685A1 (en) 2011-09-13 2013-03-21 Monsanto Technology Llc Methods and compositions for weed control comprising topical application of a glutamine synthetase polynucleotide
US10760086B2 (en) 2011-09-13 2020-09-01 Monsanto Technology Llc Methods and compositions for weed control
UY34328A (es) 2011-09-13 2013-04-30 Monsanto Technology Llc ?composiciones y métodos para controlar malezas comprendiendo un polinucleótido y agente de transferencia, y que modulan protoporfirinógeno ix oxidasa?.
UA116089C2 (uk) 2011-09-13 2018-02-12 Монсанто Текнолоджи Ллс Спосіб та композиція для боротьби з бур'янами (варіанти)
US10806146B2 (en) 2011-09-13 2020-10-20 Monsanto Technology Llc Methods and compositions for weed control
US9920326B1 (en) 2011-09-14 2018-03-20 Monsanto Technology Llc Methods and compositions for increasing invertase activity in plants
CA2850571C (en) 2011-10-04 2021-01-05 Icon Genetics Gmbh Nicotiana benthamiana plants deficient in fucosyltransferase activity
EP2766486A1 (en) 2011-10-12 2014-08-20 Bayer CropScience AG Plants with decreased activity of a starch dephosphorylating enzyme
US20140251317A1 (en) 2011-10-12 2014-09-11 Bayer Cropscience Ag Plants with decreased activity of a starch dephosphorylating enzyme
BR112014010537A2 (pt) 2011-10-31 2017-05-02 Pioneer Hi Bred Int método para modular a sensibilidade ao etileno, planta transgênica, proteína isolada, sequência de polinucleotídeos isolada, polipeptídeo com atividade regulatória de etileno, método para aumentar o rendimento em uma planta, método para melhorar um parâmetro agronômico de uma planta, método de seleção assistida por marcador de uma planta
BR112014010546B1 (pt) 2011-11-02 2021-04-13 Ceres, Inc Método de aumento do rendimento de planta em solo contendo níveis elevados de al3+, método de aumento da tolerância de uma planta
BR112014013853A2 (pt) 2011-12-09 2020-01-14 Ceres Inc uso de um ácido nucleico isolado, método de obtenção de uma célula vegetal transgênica, método de obtenção de uma planta transgênica, método de produção de uma planta, método de produção de biomassa, método de processamento de biomassa, método de alteração da composição de biomassa, método de modulação de composição de biomassa, método de produção de um produto de forragem
UA116097C2 (uk) 2011-12-11 2018-02-12 Зе Стейт Оф Ізраел, Міністрі Оф Агрікалче Енд Руерал Девелопмент, Агрікалчерал Рісьоч Організейшн, (А.Р.О.), Волкані Сентре Спосіб модуляції провідності устячка рослини
CN104703998B (zh) 2012-03-13 2020-08-21 先锋国际良种公司 植物中雄性育性的遗传减少
WO2013138309A1 (en) 2012-03-13 2013-09-19 Pioneer Hi-Bred International, Inc. Genetic reduction of male fertility in plants
US10100321B2 (en) 2012-04-19 2018-10-16 Temasek Life Sciences Laboratory Limited Methods for increasing cotton fiber length
UY34822A (es) 2012-05-24 2013-12-31 Seeds Ltd Ab Composiciones y métodos para silenciar la expresión genética
EP2870248A1 (en) 2012-07-06 2015-05-13 Bayer CropScience NV Brassica rod1 gene sequences and uses thereof
WO2014006162A1 (en) 2012-07-06 2014-01-09 Bayer Cropscience Nv Brassica plants with modified seed oil composition
BR112015000058A2 (pt) 2012-07-06 2017-08-08 Bayer Cropscience Nv sequências de genes rod1 de soja e usos das mesmas
WO2014027021A1 (en) 2012-08-16 2014-02-20 Vib Vzw Means and methods for altering the lignin pathway in plants
MX364070B (es) 2012-10-18 2019-04-10 Monsanto Technology Llc Métodos y composiciones para el control de plagas vegetales.
UY35252A (es) 2013-01-01 2014-07-31 Seeds Ltd Ab MÉTODOS PARA INTRODUCIR dsRNA EN SEMILLAS DE PLANTAS PARA MODULAR LA EXPRESIÓN GENÉTICA
US10683505B2 (en) 2013-01-01 2020-06-16 Monsanto Technology Llc Methods of introducing dsRNA to plant seeds for modulating gene expression
US10000767B2 (en) 2013-01-28 2018-06-19 Monsanto Technology Llc Methods and compositions for plant pest control
WO2014118123A1 (en) 2013-01-29 2014-08-07 The University Court Of The University Of Glasgow Methods and means for increasing stress tolerance and biomass in plants
US20160002648A1 (en) 2013-03-11 2016-01-07 Mei Guo Genes for improving nutrient uptake and abiotic stress tolerance in plants
UY35385A (es) 2013-03-13 2014-09-30 Monsanto Technology Llc ?métodos y composiciones para el control de malezas?.
WO2014164074A1 (en) 2013-03-13 2014-10-09 Pioneer Hi-Bred International, Inc. Enhanced nitrate uptake and nitrate translocation by over-expressing maize functional low-affinity nitrate transporters in transgenic maize
WO2014164116A1 (en) 2013-03-13 2014-10-09 Pioneer Hi-Bred International, Inc. Functional expression of bacterial major facilitator superfamily (sfm) gene in maize to improve agronomic traits and grain yield
EP3604535A3 (en) 2013-03-13 2020-04-22 Monsanto Technology LLC Methods and compositions for weed control
CN105143248A (zh) 2013-03-14 2015-12-09 先锋国际良种公司 玉蜀黍胁迫相关转录因子18及其用途
US20140283211A1 (en) 2013-03-14 2014-09-18 Monsanto Technology Llc Methods and Compositions for Plant Pest Control
US10568328B2 (en) 2013-03-15 2020-02-25 Monsanto Technology Llc Methods and compositions for weed control
US20140283216A1 (en) 2013-03-15 2014-09-18 Pioneer Hi Bred International Inc Compositions and methods of use of acc oxidase polynucleotides and polypeptides
US10017778B2 (en) 2013-03-21 2018-07-10 Vib Vzw Means and methods for the reduction of photorespiration in crops
AU2014278519B2 (en) 2013-06-11 2020-09-10 Syngenta Participations Ag Methods for generating transgenic plants
US10472645B2 (en) 2013-07-01 2019-11-12 Basf Se Methods and means for modulating flowering time in monocot plants
RU2703498C2 (ru) 2013-07-19 2019-10-17 Монсанто Текнолоджи Ллс Композиции и способы борьбы с leptinotarsa
US9850496B2 (en) 2013-07-19 2017-12-26 Monsanto Technology Llc Compositions and methods for controlling Leptinotarsa
UA116400C2 (uk) 2013-09-24 2018-03-12 Байєр Кропсайєнс Нв Білок, що має целюлоза:ксилоглюкан ендотрансглюкозилазну активність, та його застосування
CA2928830A1 (en) 2013-10-29 2015-05-07 Shai J. Lawit Self-reproducing hybrid plants
CA2929533C (en) 2013-11-04 2023-06-06 Monsanto Technology Llc Compositions and methods for controlling arthropod parasite and pest infestations
UA119253C2 (uk) 2013-12-10 2019-05-27 Біолоджикс, Інк. Спосіб боротьби із вірусом у кліща varroa та у бджіл
UA121462C2 (uk) 2014-01-15 2020-06-10 Монсанто Текнолоджі Елелсі Спосіб та композиція для боротьби із бур'янами з використанням полінуклеотидів epsps
US20170166920A1 (en) 2014-01-30 2017-06-15 Two Blades Foundation Plants with enhanced resistance to phytophthora
US11525143B2 (en) 2014-02-28 2022-12-13 Universidade Federal Do Rio De Janeiro Method for promoting an increase in plant biomass, productivity, and drought resistance
BR102014004881A2 (pt) 2014-02-28 2016-02-02 Univ Rio De Janeiro método para promover um aumento da biomassa e produtividade vegetal e da tolerância à seca
CN106413390B (zh) 2014-04-01 2019-09-27 孟山都技术公司 用于控制虫害的组合物和方法
US9101100B1 (en) 2014-04-30 2015-08-11 Ceres, Inc. Methods and materials for high throughput testing of transgene combinations
WO2015171603A1 (en) 2014-05-06 2015-11-12 Two Blades Foundation Methods for producing plants with enhanced resistance to oomycete pathogens
CA2953347A1 (en) 2014-06-23 2015-12-30 Monsanto Technology Llc Compositions and methods for regulating gene expression via rna interference
WO2015200539A1 (en) 2014-06-25 2015-12-30 Monsanto Technology Llc Methods and compositions for delivering nucleic acids to plant cells and regulating gene expression
MX395280B (es) 2014-06-27 2025-03-19 National Res Council Of Canada Nrc Sintasa del acido cannabicromenico proveniente de cannabis sativa.
US20170159065A1 (en) 2014-07-08 2017-06-08 Vib Vzw Means and methods to increase plant yield
US11060101B2 (en) 2014-07-10 2021-07-13 Benson Hill, Inc. Compositions and methods for increasing plant growth and yield
WO2016011179A2 (en) 2014-07-15 2016-01-21 Ceres, Inc. Methods of increasing crop yield under abiotic stress
RU2754955C2 (ru) 2014-07-29 2021-09-08 Монсанто Текнолоджи Ллс Композиции и способы борьбы с насекомыми-вредителями
CN104232796A (zh) * 2014-09-22 2014-12-24 北京出入境检验检疫局检验检疫技术中心 检测澳大利亚葡萄类病毒的RT-qPCR寡核苷酸及检测方法
WO2016050512A1 (en) 2014-10-03 2016-04-07 Bayer Cropscience Nv Methods and means for increasing stress tolerance and biomass in plants
EP3209775A4 (en) 2014-10-22 2018-09-12 Temasek Life Sciences Laboratory Limited Terpene synthases from ylang ylang (cananga odorata var. fruticosa)
WO2016113333A1 (en) 2015-01-16 2016-07-21 Bayer Cropscience Nv Leaf-preferential promoters and uses thereof
BR112017015705A2 (pt) 2015-01-22 2018-03-20 Monsanto Technology Llc composições e métodos para controle de leptinotarsa
WO2016128519A1 (en) 2015-02-12 2016-08-18 Bayer Cropscience Nv Shoot apex-preferential promoters and uses thereof
US10883103B2 (en) 2015-06-02 2021-01-05 Monsanto Technology Llc Compositions and methods for delivery of a polynucleotide into a plant
WO2016196782A1 (en) 2015-06-03 2016-12-08 Monsanto Technology Llc Methods and compositions for introducing nucleic acids into plants
CN107849568B (zh) 2015-08-07 2021-10-26 拜尔作物科学公司 根优先和胁迫诱导型启动子及其用途
WO2017062790A1 (en) 2015-10-09 2017-04-13 Two Blades Foundation Cold shock protein receptors and methods of use
US20190153456A1 (en) 2015-10-16 2019-05-23 Bayer Cropscience Nv Brassica plants with altered properties in seed production
WO2017070458A2 (en) 2015-10-23 2017-04-27 Donald Danforth Plant Science Center Genes encoding c4 transporter proteins for use in increasing crop plant yield
CN105463011B (zh) * 2015-12-22 2019-02-01 华南农业大学 一种植物生长负调控顺式元件se1及其在调控植物株高中的应用
AU2017234920B2 (en) 2016-03-18 2023-08-17 E. I. Du Pont De Nemours And Company Methods and compositions for producing clonal, non-reduced, non-recombined gametes
CA3018306A1 (en) 2016-03-24 2017-09-28 Universiteit Leiden Methods for transfecting plants and for reducing random integration events
AU2017249283A1 (en) 2016-04-11 2018-10-25 Bayer Cropscience Nv Seed-specific and endosperm-preferential promoters and uses thereof
WO2017178318A1 (en) 2016-04-11 2017-10-19 Bayer Cropscience Nv Seed-specific and endosperm-preferential promoters and uses thereof
WO2017178368A1 (en) 2016-04-13 2017-10-19 Bayer Cropscience Nv Seed-specific and embryo-preferential promoters and uses thereof
BR112018071095A2 (pt) 2016-04-13 2019-02-26 Bayer Cropscience Nv promotores preferenciais em semente e funículo e usos dos mesmos
US11542519B2 (en) 2017-03-23 2023-01-03 Basf Se Anther-specific promoter and uses thereof
BR112019024630A2 (pt) 2017-05-24 2020-06-23 BASF Agricultural Solutions Seed US LLC Ácido nucleico isolado, gene recombinante, célula hospedeira, planta, partes e sementes de plantas, planta ou célula vegetal ou parte de planta ou semente, métodos para produzir uma planta transgênica e para efetuar a expressão induzível e uso do ácido nucleico
US20210032651A1 (en) 2017-10-24 2021-02-04 Basf Se Improvement of herbicide tolerance to hppd inhibitors by down-regulation of putative 4-hydroxyphenylpyruvate reductases in soybean
BR112020008092A2 (pt) 2017-10-24 2020-09-15 BASF Agricultural Solutions Seed US LLC método para conferir tolerância a um herbicida e planta de soja transgênica
EP3502259A1 (en) 2017-12-19 2019-06-26 Universiteit Leiden A combinational strategy for reducing random integration events when transfecting plants
EP3814490A2 (en) 2018-06-27 2021-05-05 Basf Se Thermostable rubisco activase and uses thereof
US11473086B2 (en) 2019-06-19 2022-10-18 Ut-Battelle, Llc Loss of function alleles of PtEPSP-TF and its regulatory targets in rice
WO2021004838A2 (en) 2019-07-05 2021-01-14 BASF Agricultural Solutions Seed US LLC Rubisco activase with reduced adp inhibition and uses thereof
CA3148950A1 (en) 2019-07-30 2021-02-04 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (A.R.O.), Volcani Center Methods of controlling cannabinoid synthesis in plants or cells and plants and cells produced thereby
CA3185019A1 (en) 2020-05-29 2021-12-02 Front Range Biosciences, Inc. Methods and compositions for pathogen detection in plants
US20240254497A1 (en) * 2020-07-20 2024-08-01 Flagship Pioneering, Inc. Viroid-derived polynucleotides for modification of plants
WO2022178538A2 (en) * 2021-02-19 2022-08-25 Purple City Labs, Inc. Detection of pathogens in plants
US20250019717A1 (en) 2021-08-06 2025-01-16 Kws Vegetables B.V. Durable downy mildew resistance in spinach
EP4408860A1 (en) 2021-10-01 2024-08-07 Basf Se Wheat plants with an increased yield
WO2023052561A1 (en) 2021-10-01 2023-04-06 Basf Se Plants with improved properties
WO2024099765A2 (en) 2022-11-10 2024-05-16 BASF Agricultural Solutions Seed US LLC Transcription regulating nucleotide sequences and methods of use
EP4477069A1 (en) 2023-06-16 2024-12-18 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Unreduced clonal gamete formation and polyploid genome design in the solanaceae
NL2035852B1 (en) 2023-09-21 2025-03-28 Academisch Ziekenhuis Leiden Enhancement of t cell mediated therapies
CN118995804B (zh) * 2024-10-23 2025-02-28 青岛农业大学 一种突变型质粒及制备方法和应用

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4873191A (en) 1981-06-12 1989-10-10 Ohio University Genetic transformation of zygotes
ATE198350T1 (de) 1983-10-20 2001-01-15 Univ New York State Res Found Regulierung der genexpression durch translationshemmung unter verwendung einer m-rns behindernden komplementären rns
US5190931A (en) 1983-10-20 1993-03-02 The Research Foundation Of State University Of New York Regulation of gene expression by employing translational inhibition of MRNA utilizing interfering complementary MRNA
US6617496B1 (en) 1985-10-16 2003-09-09 Monsanto Company Effecting virus resistance in plants through the use of negative strand RNAs
IL81737A (en) 1986-03-28 1992-11-15 Calgene Inc Regulation of gene expression in plant cells
US5037745A (en) 1986-04-01 1991-08-06 University Of Medicine And Dentistry Of New Jersey Plasmid for the overproduction of bacteriophage T3 RNA polymerase, transcription vectors that carry a promoter recognized by its polymerase, gene coding for T3 RNA polymerase and application of these plasmids
AU2811889A (en) 1987-10-20 1989-05-23 Plant Genetic Systems N.V. A process for the production of biologically active peptide via the expression of modified storage seed protein genes in transgenic plants
GB8810120D0 (en) 1988-04-28 1988-06-02 Plant Genetic Systems Nv Transgenic nuclear male sterile plants
AU1192392A (en) 1991-02-08 1992-09-07 Plant Genetic Systems N.V. Stamen-specific promoters from rice
US5908779A (en) 1993-12-01 1999-06-01 University Of Connecticut Targeted RNA degradation using nuclear antisense RNA
ATE266734T1 (de) 1994-08-30 2004-05-15 Commw Scient Ind Res Org Pflanzentraskriptionsregulator von circovirus
ATE331034T1 (de) 1995-10-06 2006-07-15 Bayer Bioscience Nv Samenstrenung resistenz
DE19631919C2 (de) 1996-08-07 1998-07-16 Deutsches Krebsforsch Anti-Sinn-RNA mit Sekundärstruktur
AU3369900A (en) * 1999-02-19 2000-09-04 General Hospital Corporation, The Gene silencing
US6423885B1 (en) 1999-08-13 2002-07-23 Commonwealth Scientific And Industrial Research Organization (Csiro) Methods for obtaining modified phenotypes in plant cells
MXPA02012733A (es) 2000-06-23 2003-05-14 Du Pont Extrusturas recombinantes y su uso en la reduccion de la expresion de genes.
WO2002000894A2 (en) 2000-06-30 2002-01-03 Cropdesign N.V. Gene silencing vector
AU2001281024A1 (en) 2000-08-02 2002-02-13 The Board Of Regents Of The University Of Nebraska Down-regulation of single genes and simultaneous down-regulation of multiple genes by nuclear localization of rna transcripts
CA2429397C (en) * 2001-01-26 2014-06-03 Commonwealth Scientific And Industrial Research Organisation Methods and means for producing efficient silencing construct using recombinational cloning

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