BR0213936A - Ensaios de ciclização enzimáticos para homocisteìna e cistationina - Google Patents

Ensaios de ciclização enzimáticos para homocisteìna e cistationina

Info

Publication number
BR0213936A
BR0213936A BR0213936-7A BR0213936A BR0213936A BR 0213936 A BR0213936 A BR 0213936A BR 0213936 A BR0213936 A BR 0213936A BR 0213936 A BR0213936 A BR 0213936A
Authority
BR
Brazil
Prior art keywords
homocysteine
cystathionine
amount
derivatives
ammonia
Prior art date
Application number
BR0213936-7A
Other languages
English (en)
Other versions
BRPI0213936B8 (pt
BRPI0213936B1 (pt
Inventor
Heather Kay Webb
Jeffrey Owens
Raymond Liedtke
Doreen Forest
Mark Legaz
Glenn Kawasaki
Sobomabo Lawson
Original Assignee
Catch Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=21756556&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR0213936(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Catch Inc filed Critical Catch Inc
Publication of BR0213936A publication Critical patent/BR0213936A/pt
Publication of BRPI0213936B1 publication Critical patent/BRPI0213936B1/pt
Publication of BRPI0213936B8 publication Critical patent/BRPI0213936B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6806—Determination of free amino acids
    • G01N33/6812—Assays for specific amino acids
    • G01N33/6815—Assays for specific amino acids containing sulfur, e.g. cysteine, cystine, methionine, homocysteine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/968—High energy substrates, e.g. fluorescent, chemiluminescent, radioactive
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/975—Kit

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

"ENSAIOS DE CICLIZAçãO ENZIMáTICA PARA HOMOCISTEìNA E CISTATIONINA". A presente invenção refere-se a um ensaio de ciclização enzimática para avaliação da quantidade de homocisteína e/ou cistationina em uma solução tal como sangue, derivados de sangue ou urina. O ensaio compreende as etapas de contato da solução contendo homocisteína e/ou cistationina para formar uma mistura de reação, com CBS, ou um seu derivado, L-serina, e CBL, ou um seu derivado, por um período de tempo suficiente para catalisar a conversão clínica da forma homocisteína em cistationina e a reconversão de cistationina em homocisteína com a produção de piruvato e amónia; determinação da quantidade de homocisteína e/ou amónia presente na mistura de reação; e determinação da quantidade de homocisteína e/ou cistationina presente na solução baseada na quantidade de piruvato e/ou amónia formada. Vetores de expressão e procedimentos de isolamento para CBS, ou seus derivados, e CBI, ou seus derivados, são também providos, bem como kits de teste para realizar o ensaio. Em modalidades preferidas, os ensaios podem ser realizados em 15 minutos ou menos, com um mínimo de uso de enzima.
BRPI0213936A 2001-11-06 2002-11-06 métodos de ensaio de ciclização enzimática para homocisteína e cistationina e de teste de amostras contendo homocisteína, bem como kit de teste para quantificar homocisteína e ensaios de avaliação da quantidade de homocisteína BRPI0213936B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/012,762 US6635438B2 (en) 1999-11-02 2001-11-06 Enzymatic cycling assays for homocysteine and cystathionine
PCT/US2002/035777 WO2003040694A2 (en) 2001-11-06 2002-11-06 Enzymatic cycling assays for homocysteine and cystathionine

Publications (3)

Publication Number Publication Date
BR0213936A true BR0213936A (pt) 2004-08-31
BRPI0213936B1 BRPI0213936B1 (pt) 2015-08-25
BRPI0213936B8 BRPI0213936B8 (pt) 2021-07-27

Family

ID=21756556

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0213936A BRPI0213936B8 (pt) 2001-11-06 2002-11-06 métodos de ensaio de ciclização enzimática para homocisteína e cistationina e de teste de amostras contendo homocisteína, bem como kit de teste para quantificar homocisteína e ensaios de avaliação da quantidade de homocisteína

Country Status (12)

Country Link
US (2) US6635438B2 (pt)
EP (1) EP1470242B1 (pt)
CN (1) CN1612937B (pt)
AU (1) AU2002352538A1 (pt)
BR (1) BRPI0213936B8 (pt)
DK (1) DK1470242T3 (pt)
HU (1) HU228343B1 (pt)
NO (1) NO20042144L (pt)
RU (1) RU2344176C2 (pt)
SK (1) SK288189B6 (pt)
WO (1) WO2003040694A2 (pt)
ZA (1) ZA200403395B (pt)

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US6635438B2 (en) * 1999-11-02 2003-10-21 Catch, Inc. Enzymatic cycling assays for homocysteine and cystathionine
GB0008784D0 (en) * 2000-04-10 2000-05-31 Axis Shield Plc Assay
GB0311804D0 (en) * 2003-05-22 2003-06-25 Univ Glasgow Improved homocysteine assay
US7097968B2 (en) * 2003-07-10 2006-08-29 General Atomics Methods and compositions for assaying homocysteine
US8476034B2 (en) * 2003-07-10 2013-07-02 General Atomics Methods and compositions for assaying homocysteine
DE10342264C5 (de) 2003-09-12 2012-10-31 Leica Biosystems Nussloch Gmbh System zum eindeutigen Zuordnen von histologischen Kassetten und Objektträgern
GB0428130D0 (en) * 2004-12-22 2005-01-26 Oxford Biosensors Ltd Selective HDL cholesterol assay
CA2618699C (en) * 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
EP1976876A4 (en) * 2005-12-22 2010-01-13 Conjuchem Biotechnologies Inc PROCESS FOR PRODUCING PREFORMED ALBUMIN CONJUGATES AND THERAPEUTIC AGENT
WO2009039298A2 (en) 2007-09-18 2009-03-26 University Of Florida Research Foundation, Inc. Sensors using aigan/gan high electron mobility transistors
WO2009091581A2 (en) * 2008-01-18 2009-07-23 Vatrix Medical, Inc. Diagnostic biomarkers for vascular aneurysm
WO2010054159A2 (en) * 2008-11-06 2010-05-14 University Of Florida Research Foundation, Inc. Materials and methods for detecting toxins, pathogens and other biological materials
RU2012132313A (ru) * 2009-12-30 2014-02-10 Зэ Стейт Оф Орегон Эктинг Бай Энд Сру Зэ Стейт Боард Оф Хайе Эдьюкейшн Он Бехалф Оф Портланд Стейт Юниверсити Обнаружение тиола
US9216209B1 (en) 2011-06-06 2015-12-22 Kilmer S. McCully Compositions and method for utilization of thioretinamide in therapy of degenerative diseases of aging
RU2471181C1 (ru) * 2011-06-20 2012-12-27 Открытое акционерное общество Научно-производственное объединение "Химавтоматика" Способ определения суммарного содержания антиоксидантов и суммарного содержания антоцианов в пищевых продуктах и напитках
US9034318B2 (en) * 2011-08-30 2015-05-19 The Regents Of The University Of Colorado, A Body Corporate Chemically modified cystathionine beta-synthase enzyme for treatment of homocystinuria
JP5914019B2 (ja) * 2012-02-03 2016-05-11 旭化成ファーマ株式会社 酵素サイクリング反応によるメバロン酸の測定方法およびその校正試料
CN104755926B (zh) 2012-08-24 2017-12-01 卡斯西部储备大学 用于检测α‑甲酰基‑CoA消旋酶(AMACR)和前列腺癌的系统和方法
CN103674873B (zh) * 2013-11-22 2015-05-20 杭州利安生物科技有限公司 一种定量检测丝氨酸的方法
CN104111338B (zh) * 2014-05-07 2016-03-02 山东博科生物产业有限公司 一种抗干扰性强的同型半胱氨酸检测试剂盒
US11009479B2 (en) 2014-05-27 2021-05-18 Case Western Reserve University Systems and methods for the detection of HbA1c
US10465229B2 (en) 2014-05-27 2019-11-05 Case Western Reserve University System and method for detecting neural injury
US10883956B2 (en) 2014-05-27 2021-01-05 Case Western Reserve University Electrochemical sensor for analyte detection
CN104198726B (zh) * 2014-08-14 2016-07-06 上海睿康生物科技有限公司 一种稳定的同型半胱氨酸检测试剂盒
CN105039368A (zh) * 2015-07-08 2015-11-11 北京嘉万生物技术有限公司 一种重组酵母胱硫醚beta合酶催化中心的表达及放大生产
CN105181970B (zh) * 2015-08-28 2017-05-03 宁波瑞源生物科技有限公司 一种稳定的同型半胱氨酸检测试剂盒
CN106970230B (zh) * 2017-03-29 2019-06-11 广州市伊川生物科技有限公司 一种同型半胱氨酸测定试剂盒
CN110346572A (zh) * 2018-04-04 2019-10-18 浙江力耐得传动科技有限公司 偶联丙酮酸氧化酶测定同型半胱氨酸（Hcy）的方法及测定试剂盒
CN108802125B (zh) * 2018-08-28 2023-09-15 长沙理工大学 基于七(6-巯基-6-去氧)-β-环糊精的L-半胱氨酸的检测方法及传感器
CN109975279B (zh) * 2019-03-07 2021-05-28 广州悦蜂生物防治科技有限公司 一种检测脂肪酶活性的方法、试剂盒及速测卡
US11697807B2 (en) 2019-09-30 2023-07-11 Case Western Reserve University Electrochemical biosensor
CN110954581A (zh) * 2019-12-19 2020-04-03 湖南海源医疗科技股份有限公司 一种快速检测同型半胱氨酸的电化学方法
CN114689415A (zh) * 2020-12-31 2022-07-01 桂林优利特医疗电子有限公司 一种稳定性好的同型半胱氨酸检测试剂盒及制备方法
CN114277021B (zh) * 2021-11-19 2022-09-20 武汉瀚海新酶生物科技有限公司 胱硫醚-β-裂解酶及其制备方法和应用
CN114807199A (zh) * 2022-02-22 2022-07-29 三诺生物传感股份有限公司 重组胱硫醚β-裂解酶的制备方法及其应用
CN115725556B (zh) * 2022-09-20 2024-05-03 青岛硕景生物科技有限公司 一种稳定性提高的突变胱硫醚β裂解酶及其制备方法和应用

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Also Published As

Publication number Publication date
HUP0500095A3 (en) 2010-03-29
AU2002352538A1 (en) 2003-05-19
US6867014B2 (en) 2005-03-15
HU228343B1 (en) 2013-03-28
BRPI0213936B8 (pt) 2021-07-27
ZA200403395B (en) 2006-06-28
RU2004117087A (ru) 2005-04-27
HUP0500095A2 (hu) 2005-05-30
WO2003040694A3 (en) 2004-08-19
BRPI0213936B1 (pt) 2015-08-25
CN1612937B (zh) 2010-05-05
RU2344176C2 (ru) 2009-01-20
SK2042004A3 (en) 2004-10-05
US6635438B2 (en) 2003-10-21
WO2003040694A2 (en) 2003-05-15
EP1470242A2 (en) 2004-10-27
US20030138872A1 (en) 2003-07-24
US20040096929A1 (en) 2004-05-20
NO20042144L (no) 2004-08-06
EP1470242B1 (en) 2013-08-14
SK288189B6 (sk) 2014-05-06
CN1612937A (zh) 2005-05-04
DK1470242T3 (da) 2013-11-11
EP1470242A4 (en) 2007-01-03

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