BR0014833A - Método para melhorar a estabilidade e ampliar a faixa de ph de famìlia de xilanases g/11 - Google Patents

Método para melhorar a estabilidade e ampliar a faixa de ph de famìlia de xilanases g/11

Info

Publication number
BR0014833A
BR0014833A BR0014833-4A BR0014833A BR0014833A BR 0014833 A BR0014833 A BR 0014833A BR 0014833 A BR0014833 A BR 0014833A BR 0014833 A BR0014833 A BR 0014833A
Authority
BR
Brazil
Prior art keywords
range
extend
improve stability
improve
xylanase
Prior art date
Application number
BR0014833-4A
Other languages
English (en)
Inventor
Fred Fenel
Ossi Turunen
Matti Leisola
Original Assignee
Carbozyme Oy
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Carbozyme Oy filed Critical Carbozyme Oy
Publication of BR0014833A publication Critical patent/BR0014833A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"MéTODO PARA MELHORAR A ESTABILIDADE E AMPLIAR A FAIXA DE PH DE XILANASES DE FAMìLIA G/11". A presente invenção refere-se a construção de proteina, e diz respeito a xilanases de família G/11 especialmente, e genes codificando as ditas enzimas. Especificamente, a invenção diz respeito ao gene XYNII de Trichoderma reesei, que codifica para endo-1,4-<225>-xilanase (EC 3.2.1.8). A invenção descreve como a mutagênese de sítio dirigido pode ser usada para melhorar as propriedades de uma enzima para compatibilizar com as condições industriais onde ela é usada. A construção de proteína pode ser usada para melhorar a termoatividade e a termoestabilidade de xilanases, assim como ampliar sua faixa de pH.
BR0014833-4A 1999-10-12 2000-10-12 Método para melhorar a estabilidade e ampliar a faixa de ph de famìlia de xilanases g/11 BR0014833A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
FI992186 1999-10-12
US16328399P 1999-11-03 1999-11-03
FI20001586A FI108728B (fi) 1999-10-12 2000-07-03 Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi
PCT/FI2000/000877 WO2001027252A1 (en) 1999-10-12 2000-10-12 Method to improve the stability and broaden the ph range of family g/11 xylanases

Publications (1)

Publication Number Publication Date
BR0014833A true BR0014833A (pt) 2002-06-18

Family

ID=39618081

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0014833-4A BR0014833A (pt) 1999-10-12 2000-10-12 Método para melhorar a estabilidade e ampliar a faixa de ph de famìlia de xilanases g/11

Country Status (16)

Country Link
US (3) US8426181B2 (pt)
EP (1) EP1222256B1 (pt)
JP (1) JP4647169B2 (pt)
CN (2) CN101173262A (pt)
AT (1) ATE295879T1 (pt)
AU (1) AU781296B2 (pt)
BR (1) BR0014833A (pt)
CA (1) CA2385937C (pt)
DE (1) DE60020255T2 (pt)
DK (1) DK1222256T3 (pt)
ES (1) ES2239046T3 (pt)
FI (1) FI108728B (pt)
IL (1) IL149061A0 (pt)
NO (1) NO20021723D0 (pt)
WO (1) WO2001027252A1 (pt)
ZA (1) ZA200202894B (pt)

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CN109402091A (zh) * 2017-08-18 2019-03-01 青岛蔚蓝生物集团有限公司 木聚糖酶突变体

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US20070243595A1 (en) * 2006-04-14 2007-10-18 Clarkson Kathleen A Modified enzymes, methods to produce modified enzymes and uses thereof
BRPI0414370A (pt) * 2003-09-15 2006-11-14 Genencor Int enzimas modificadas, processos para produzir enzimas modificadas e usos das mesmas
EP2497377A3 (en) 2005-10-12 2013-09-04 Danisco US Inc. Stable, durable granules with active agents
WO2007115391A1 (en) * 2006-04-12 2007-10-18 National Research Council Of Cananda Modification of xylanases to increase thermophilicity, thermostability and alkalophilicity
RU2464313C2 (ru) * 2006-04-12 2012-10-20 Нэйшенл Рисерч Каунсил Оф Кэнэда Модифицированная ксиланаза
CN101289668B (zh) * 2007-04-16 2010-09-08 屏东科技大学 高酸碱反应范围的聚木醣酶的突变基因及其点突变方法
WO2008150376A1 (en) 2007-05-21 2008-12-11 Danisco Us, Inc., Genencor Division Use of an aspartic protease (nsp24) signal sequence for heterologous protein expression
WO2012019169A1 (en) 2010-08-06 2012-02-09 Danisco Us Inc. Production of isoprene under neutral ph conditions
AR082578A1 (es) 2010-08-06 2012-12-19 Danisco Us Inc SACARIFICACION Y FERMENTACION A pH NEUTRO
EP2739742A1 (en) 2011-08-05 2014-06-11 Danisco US Inc. PRODUCTION OF ISOPRENOIDS UNDER NEUTRAL pH CONDITIONS
EP3342867B1 (en) 2011-11-25 2020-07-29 Mitsui Chemicals, Inc. Mutant xylanase, manufacturing method and use therefor, and method for manufacturing saccharified lignocellulose
US20140329309A1 (en) 2011-12-09 2014-11-06 Danisco Us Inc. Ribosomal Promoters for Production in Microorganisms
WO2013192043A1 (en) 2012-06-20 2013-12-27 Danisco Us Inc. Sandwich granule
CN104470371A (zh) 2012-07-18 2015-03-25 丹尼斯科美国公司 熔化延迟的颗粒
EP3058055A1 (en) 2013-10-15 2016-08-24 Danisco US Inc. Clay granule
BR112016009416A2 (pt) 2013-10-28 2017-10-03 Danisco Us Inc Levedura seca ativa geneticamente modificada de grande escala
CN103627686B (zh) * 2013-11-14 2015-03-18 青岛蔚蓝生物集团有限公司 一种木聚糖酶突变体及其应用
EP3068879B1 (en) 2013-11-14 2019-12-25 Danisco US Inc. Stable enzymes by glycation reduction
CN103642777A (zh) * 2013-12-10 2014-03-19 江南大学 一种提高米曲霉木聚糖酶热稳定性的方法
GB201401648D0 (en) * 2014-01-31 2014-03-19 Dupont Nutrition Biosci Aps Protein
US9681386B2 (en) * 2014-03-06 2017-06-13 Apple Inc. Wi-Fi low energy preamble
WO2016072448A1 (ja) * 2014-11-05 2016-05-12 東レ株式会社 エンドキシラナーゼ変異体、バイオマス分解用酵素組成物及び糖液の製造方法
CN104560920B (zh) * 2015-01-26 2017-07-18 青岛蔚蓝生物集团有限公司 一种酸性木聚糖酶突变体及其应用
CN107250365A (zh) 2015-02-19 2017-10-13 丹尼斯科美国公司 增强的蛋白质表达
EP3270893B1 (en) 2015-03-19 2021-07-28 Danisco US Inc. Stable granules with low internal water activity
EP3192866A1 (en) 2016-01-15 2017-07-19 CIC nanoGUNE - Asociación Centro de Investigación Cooperativa en Nanociencias Endocellulases and uses thereof
CN105524904B (zh) * 2016-02-29 2019-03-08 四川农业大学 一种耐热的重组木聚糖酶及其制备方法和用途
DK3419991T3 (da) 2016-03-04 2023-01-16 Danisco Us Inc Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer
WO2019089898A1 (en) 2017-11-02 2019-05-09 Danisco Us Inc Freezing point depressed solid matrix compositions for melt granulation of enzymes
EP3502126A1 (en) 2017-12-19 2019-06-26 CIC nanoGUNE - Asociación Centro de Investigación Cooperativa en Nanociencias Ancestral cellulases and uses thereof
CN109320593A (zh) * 2018-11-05 2019-02-12 中国人民解放军军事科学院军事医学研究院 抑制hiv感染的螺旋多肽及其用途
EP3872173A1 (en) 2020-02-28 2021-09-01 CIC nanoGUNE - Asociación Centro de Investigación Cooperativa en Nanociencias Method for producing crystalline nanocellulose
EP3872172A1 (en) 2020-02-28 2021-09-01 CIC nanoGUNE - Asociación Centro de Investigación Cooperativa en Nanociencias Conductive cellulose composite materials and uses thereof
KR20230021903A (ko) * 2021-08-06 2023-02-14 씨제이제일제당 (주) 자일라나제 활성을 갖는 변이형 폴리펩티드

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FI108728B (fi) * 1999-10-12 2002-03-15 Carbozyme Oy Menetelmä perheen G/11 ksylanaasien stabiilisuuden parantamiseksi ja optimaalisen pH-alueen muuttamiseksi
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109402091A (zh) * 2017-08-18 2019-03-01 青岛蔚蓝生物集团有限公司 木聚糖酶突变体

Also Published As

Publication number Publication date
ES2239046T3 (es) 2005-09-16
CN101173262A (zh) 2008-05-07
US20130288335A1 (en) 2013-10-31
EP1222256B1 (en) 2005-05-18
WO2001027252A1 (en) 2001-04-19
CN100378217C (zh) 2008-04-02
AU7925000A (en) 2001-04-23
NO20021723L (no) 2002-04-11
ZA200202894B (en) 2003-04-14
US8426181B2 (en) 2013-04-23
US20080171374A1 (en) 2008-07-17
US9481874B2 (en) 2016-11-01
DE60020255D1 (de) 2005-06-23
JP2003511066A (ja) 2003-03-25
NO20021723D0 (no) 2002-04-11
DK1222256T3 (da) 2005-09-05
FI108728B (fi) 2002-03-15
CA2385937C (en) 2010-07-27
JP4647169B2 (ja) 2011-03-09
ATE295879T1 (de) 2005-06-15
US8846364B2 (en) 2014-09-30
CN1379813A (zh) 2002-11-13
CA2385937A1 (en) 2001-04-19
FI20001586A0 (fi) 2000-07-03
EP1222256A1 (en) 2002-07-17
IL149061A0 (en) 2002-11-10
AU781296B2 (en) 2005-05-12
US20150011743A1 (en) 2015-01-08
DE60020255T2 (de) 2006-01-19

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Legal Events

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B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]
B25A Requested transfer of rights approved

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