BG45047A3 - Метод за получаване на изопеницилин н синтетазна активност в гостоприемникова клетка - Google Patents
Метод за получаване на изопеницилин н синтетазна активност в гостоприемникова клеткаInfo
- Publication number
- BG45047A3 BG45047A3 BG074576A BG7457686A BG45047A3 BG 45047 A3 BG45047 A3 BG 45047A3 BG 074576 A BG074576 A BG 074576A BG 7457686 A BG7457686 A BG 7457686A BG 45047 A3 BG45047 A3 BG 45047A3
- Authority
- BG
- Bulgaria
- Prior art keywords
- host cell
- isopenicillin
- synthetase activity
- obtaining
- encoding
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 230000000694 effects Effects 0.000 title abstract 2
- 229930191709 Isopenicillin Natural products 0.000 title 1
- 102000003960 Ligases Human genes 0.000 title 1
- 108090000364 Ligases Proteins 0.000 title 1
- 108030003691 Isopenicillin-N synthases Proteins 0.000 abstract 1
- 239000003242 anti bacterial agent Substances 0.000 abstract 1
- 229940088710 antibiotic agent Drugs 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000003814 drug Substances 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 108010012172 isopenicillin N synthetase Proteins 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 230000029279 positive regulation of transcription, DNA-dependent Effects 0.000 abstract 1
- 238000003259 recombinant expression Methods 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
- C12N15/69—Increasing the copy number of the vector
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US72587085A | 1985-04-22 | 1985-04-22 | |
| US79938485A | 1985-11-18 | 1985-11-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BG45047A3 true BG45047A3 (bg) | 1989-03-15 |
Family
ID=27111238
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BG074576A BG45047A3 (bg) | 1985-04-22 | 1986-04-18 | Метод за получаване на изопеницилин н синтетазна активност в гостоприемникова клетка |
| BG078442A BG45219A3 (bg) | 1985-04-22 | 1986-04-18 | Метод за получаване на вектор на рекомбинантна днк,съдържащ част от изопеницилин синтеза на цефалоспориум акремониум |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BG078442A BG45219A3 (bg) | 1985-04-22 | 1986-04-18 | Метод за получаване на вектор на рекомбинантна днк,съдържащ част от изопеницилин синтеза на цефалоспориум акремониум |
Country Status (14)
| Country | Link |
|---|---|
| EP (1) | EP0200425B1 (it) |
| JP (1) | JPH0787788B2 (it) |
| KR (1) | KR860008287A (it) |
| CN (1) | CN86102604A (it) |
| AU (2) | AU592791B2 (it) |
| BG (2) | BG45047A3 (it) |
| CA (1) | CA1309673C (it) |
| DE (1) | DE3683080D1 (it) |
| DK (1) | DK178386A (it) |
| ES (3) | ES8800344A1 (it) |
| FI (1) | FI95810C (it) |
| HU (1) | HU202279B (it) |
| IE (1) | IE58780B1 (it) |
| IL (1) | IL78531A0 (it) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3177213D1 (de) | 1980-04-03 | 1990-10-18 | Biogen Inc | Dns-sequenzen, rekombinante dns-molekuele und verfahren zur herstellung von dem menschlichen fibroblast-interferon. |
| GB8508800D0 (en) * | 1985-04-04 | 1985-05-09 | Glaxo Group Ltd | Microbiological process |
| US4892819A (en) * | 1985-11-25 | 1990-01-09 | Eli Lilly And Company | Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum |
| DE3789880T2 (de) * | 1986-01-31 | 1994-09-08 | Beecham Group Plc | Isolierung und Expression von bei der Biosynthese von Beta-Laktamen beteiligten Genen. |
| CA1327170C (en) * | 1987-03-04 | 1994-02-22 | Stephen Wyatt Queener | Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase and deacetylcephalosporin c synthetase |
| GB2208077A (en) * | 1987-04-24 | 1989-02-22 | Antibioticos Sa | Isopenicillin n synthetase gene derived from aspergillus nidulans |
| EP0303364A1 (en) * | 1987-08-10 | 1989-02-15 | The Governors of the University of Alberta | Cloning and nucleotide sequence determination of the isopenicillin N synthetase gene from streptomyces clavuligerus |
| US4885252A (en) * | 1987-09-08 | 1989-12-05 | Eli Lilly And Company | Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from aspergillus nidulans |
| US4950603A (en) * | 1987-11-02 | 1990-08-21 | Eli Lilly And Company | Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from Streptomyces lipmanii |
| US6258555B1 (en) | 1987-12-09 | 2001-07-10 | Beecham Group P.L.C. | DNA encoding ACV synthetase |
| GB8728811D0 (en) * | 1987-12-09 | 1988-01-27 | Beecham Group Plc | Novel substance |
| KR0171897B1 (ko) * | 1989-12-27 | 1999-02-01 | 후지사와 도모끼찌로 | 7-아미노세펨 화합물 또는 그 염류의 제조 방법 |
| ATE180279T1 (de) * | 1990-01-12 | 1999-06-15 | Glaxo Group Ltd | Für ein enzym der antibiotika-biosynthese kodierende dna-sequenz |
| JP3764166B2 (ja) * | 1994-09-28 | 2006-04-05 | ノボザイムス アクティーゼルスカブ | 二次代謝産物を産生するための方法 |
| DE19755302A1 (de) * | 1997-12-12 | 1999-06-17 | Hoechst Marion Roussel De Gmbh | Neuer Transkriptionsfaktor CPCR1 zur Regulation des Isopenicillin N-Synthetase-Gens (pcbC) und des AC-/ACV-Synthetase-Gens (pcbAB) |
| AU4181000A (en) * | 1999-04-02 | 2000-10-23 | Du Pont Pharmaceuticals Company | Novel lactam inhibitors of matrix metalloproteinases, tnf-alpha, and aggrecanase |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3330003A1 (de) * | 1982-10-21 | 1984-10-31 | Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig | Dna-sequenz und dna-strukturen und sie verwendendes verfahren zur herstellung von penicillin-acylase |
| CA1278540C (en) * | 1983-07-22 | 1991-01-02 | Eli Lilly And Company | Modified antibiotic resistance gene |
| US4762786A (en) * | 1984-09-27 | 1988-08-09 | Eli Lilly And Company | Vectors and conditions which allow genetic transformation of cephalosporium |
| GB8508800D0 (en) * | 1985-04-04 | 1985-05-09 | Glaxo Group Ltd | Microbiological process |
| US4892819A (en) * | 1985-11-25 | 1990-01-09 | Eli Lilly And Company | Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum |
-
1986
- 1986-04-17 CA CA000506967A patent/CA1309673C/en not_active Expired - Lifetime
- 1986-04-17 DE DE8686302873T patent/DE3683080D1/de not_active Expired - Lifetime
- 1986-04-17 IL IL78531A patent/IL78531A0/xx not_active IP Right Cessation
- 1986-04-17 EP EP86302873A patent/EP0200425B1/en not_active Expired - Lifetime
- 1986-04-18 DK DK178386A patent/DK178386A/da not_active Application Discontinuation
- 1986-04-18 BG BG074576A patent/BG45047A3/xx unknown
- 1986-04-18 BG BG078442A patent/BG45219A3/xx unknown
- 1986-04-18 FI FI861653A patent/FI95810C/fi not_active IP Right Cessation
- 1986-04-18 IE IE102986A patent/IE58780B1/en not_active IP Right Cessation
- 1986-04-21 JP JP61093178A patent/JPH0787788B2/ja not_active Expired - Fee Related
- 1986-04-21 CN CN198686102604A patent/CN86102604A/zh active Pending
- 1986-04-21 AU AU56431/86A patent/AU592791B2/en not_active Ceased
- 1986-04-21 HU HU861666A patent/HU202279B/hu not_active IP Right Cessation
- 1986-04-21 ES ES554218A patent/ES8800344A1/es not_active Expired
- 1986-04-21 KR KR1019860003061A patent/KR860008287A/ko not_active Application Discontinuation
-
1987
- 1987-08-11 ES ES557658A patent/ES8802585A1/es not_active Expired
-
1988
- 1988-04-15 ES ES557829A patent/ES8900142A1/es not_active Expired
-
1989
- 1989-09-27 AU AU42347/89A patent/AU619596B2/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| AU4234789A (en) | 1990-01-11 |
| AU5643186A (en) | 1986-10-30 |
| HUT40705A (en) | 1987-01-28 |
| ES8802585A1 (es) | 1988-08-16 |
| FI95810B (fi) | 1995-12-15 |
| EP0200425A3 (en) | 1988-07-06 |
| DK178386D0 (da) | 1986-04-18 |
| IL78531A0 (en) | 1986-08-31 |
| ES554218A0 (es) | 1987-11-01 |
| ES557829A0 (es) | 1989-01-16 |
| FI861653A0 (fi) | 1986-04-18 |
| IE58780B1 (en) | 1993-11-17 |
| EP0200425A2 (en) | 1986-11-05 |
| CN86102604A (zh) | 1986-11-26 |
| ES8900142A1 (es) | 1989-01-16 |
| JPS61254192A (ja) | 1986-11-11 |
| AU619596B2 (en) | 1992-01-30 |
| JPH0787788B2 (ja) | 1995-09-27 |
| FI95810C (fi) | 1996-03-25 |
| KR860008287A (ko) | 1986-11-14 |
| EP0200425B1 (en) | 1991-12-27 |
| DK178386A (da) | 1986-10-23 |
| CA1309673C (en) | 1992-11-03 |
| FI861653A (fi) | 1986-10-23 |
| HU202279B (en) | 1991-02-28 |
| AU592791B2 (en) | 1990-01-25 |
| IE861029L (en) | 1986-10-22 |
| ES8800344A1 (es) | 1987-11-01 |
| ES557658A0 (es) | 1988-08-16 |
| BG45219A3 (bg) | 1989-04-14 |
| DE3683080D1 (de) | 1992-02-06 |
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