AU702896B2 - Detection of nucleic acids in cells by thermophilic strand displacement amplification - Google Patents

Detection of nucleic acids in cells by thermophilic strand displacement amplification Download PDF

Info

Publication number
AU702896B2
AU702896B2 AU70192/96A AU7019296A AU702896B2 AU 702896 B2 AU702896 B2 AU 702896B2 AU 70192/96 A AU70192/96 A AU 70192/96A AU 7019296 A AU7019296 A AU 7019296A AU 702896 B2 AU702896 B2 AU 702896B2
Authority
AU
Australia
Prior art keywords
target sequence
amplification
amplification primer
seq
restriction endonuclease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU70192/96A
Other languages
English (en)
Other versions
AU7019296A (en
Inventor
Kenton L Lohman
Natalie V. Ostrerova
Robert Alan Reid
Mark Van Cleve
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Becton Dickinson and Co
Original Assignee
Becton Dickinson and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/531,747 external-priority patent/US5631147A/en
Priority claimed from US08/531,749 external-priority patent/US5733752A/en
Application filed by Becton Dickinson and Co filed Critical Becton Dickinson and Co
Publication of AU7019296A publication Critical patent/AU7019296A/en
Application granted granted Critical
Publication of AU702896B2 publication Critical patent/AU702896B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701Specific hybridization probes
    • C12Q1/702Specific hybridization probes for retroviruses
    • C12Q1/703Viruses associated with AIDS
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU70192/96A 1995-09-21 1996-09-12 Detection of nucleic acids in cells by thermophilic strand displacement amplification Ceased AU702896B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US08/531747 1995-09-21
US08/531,747 US5631147A (en) 1995-09-21 1995-09-21 Detection of nucleic acids in cells by thermophilic strand displacement amplification
US08/531749 1995-09-21
US08/531,749 US5733752A (en) 1993-12-10 1995-09-21 Detection of nucleic acids in cells by thermophilic strand displacement amplification
PCT/US1996/014648 WO1997011196A2 (en) 1995-09-21 1996-09-12 Detection of nucleic acids in cells by thermophilic strand displacement amplification

Publications (2)

Publication Number Publication Date
AU7019296A AU7019296A (en) 1997-04-09
AU702896B2 true AU702896B2 (en) 1999-03-11

Family

ID=27063626

Family Applications (1)

Application Number Title Priority Date Filing Date
AU70192/96A Ceased AU702896B2 (en) 1995-09-21 1996-09-12 Detection of nucleic acids in cells by thermophilic strand displacement amplification

Country Status (6)

Country Link
EP (1) EP0796347A2 (ja)
JP (2) JP3092163B2 (ja)
AU (1) AU702896B2 (ja)
BR (1) BR9606653A (ja)
CA (1) CA2204641A1 (ja)
WO (1) WO1997011196A2 (ja)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5916779A (en) * 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US6187567B1 (en) * 1999-09-01 2001-02-13 Akzo Nobel N.V. Methods and reagents for in situ amplification
JP2002159295A (ja) * 2000-11-27 2002-06-04 Keio Gijuku Hiv−1rna−dnaハイブリッドの定量法および診断キット
ATE319731T1 (de) * 2001-04-13 2006-03-15 Becton Dickinson Co Amplifikation und nachweis von mycoplasma pneumoniae
US7112423B2 (en) 2001-07-15 2006-09-26 Keck Graduate Institute Nucleic acid amplification using nicking agents
BR0211155A (pt) 2001-07-15 2005-02-01 Keck Graduate Inst Amplificação de fragmentos de ácido nucléico com uso de agentes de corte
WO2004097029A2 (en) 2003-04-25 2004-11-11 Becton, Dickinson And Company Detection of herpes simplex virus types 1 and 2 by nucleic acid amplification
WO2005075680A1 (ja) * 2004-02-09 2005-08-18 Fuso Pharmaceutical Industries, Ltd. 核酸検出方法およびその利用
FR2878254B1 (fr) * 2004-11-22 2010-08-20 Bio Rad Pasteur Composition pour l'amplification d'acides nucleiques
CN102575220B (zh) 2009-09-03 2015-09-16 贝克顿·迪金森公司 用于直接化学裂解的方法和组合物
CN112391450A (zh) * 2020-11-19 2021-02-23 四川大学 一种基于修饰dNTP的高特异和高灵敏核酸检测方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1284931C (en) * 1986-03-13 1991-06-18 Henry A. Erlich Process for detecting specific nucleotide variations and genetic polymorphisms present in nucleic acids
US5043272A (en) * 1989-04-27 1991-08-27 Life Technologies, Incorporated Amplification of nucleic acid sequences using oligonucleotides of random sequence as primers
GB9116042D0 (en) * 1991-07-24 1991-09-11 Royal Free Hosp School Med Diagnostic method
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
CA2159103C (en) * 1993-03-26 2002-03-12 Sherrol H. Mcdonough Detection of human immunodeficiency virus type 1
US5523204A (en) * 1993-12-10 1996-06-04 Becton Dickinson And Company Detection of nucleic acids in cells by strand displacement amplification
KR100230718B1 (ko) * 1994-03-16 1999-11-15 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 등온 가닥 변위 핵산 증폭법

Also Published As

Publication number Publication date
JPH10500313A (ja) 1998-01-13
MX9703730A (es) 1997-09-30
JP3092163B2 (ja) 2000-09-25
WO1997011196A2 (en) 1997-03-27
EP0796347A2 (en) 1997-09-24
JP2000300281A (ja) 2000-10-31
CA2204641A1 (en) 1997-03-27
BR9606653A (pt) 1997-11-04
WO1997011196A3 (en) 1997-05-09
AU7019296A (en) 1997-04-09

Similar Documents

Publication Publication Date Title
US5733752A (en) Detection of nucleic acids in cells by thermophilic strand displacement amplification
US5631147A (en) Detection of nucleic acids in cells by thermophilic strand displacement amplification
EP0878553B1 (en) Strand displacement amplification of RNA targets
EP0428197B1 (en) Methods of extracting nucleic acids and PCR amplification without using a proteolytic enzyme
JP2005511030A (ja) 核酸の増幅方法
AU702896B2 (en) Detection of nucleic acids in cells by thermophilic strand displacement amplification
US20020137039A1 (en) 5' Nuclease nucleic acid amplification assay having an improved internal control
CN106715718A (zh) 用于检测和分析结核分枝杆菌的组合物和方法
EP0805213A2 (en) Detection of mycobacteria by multiplex nucleic acid amplification
US9121054B2 (en) Detection of nucleic acid amplification products in the presence of an internal control sequence on an immunochromatographic strip
US20230416824A1 (en) Systems for the detection of targeted gene variations and viral genomes and methods of producing and using same
EP0753074B1 (en) Method for reducing background signals in dna replication/detection assays
CA2305858A1 (en) Detection of nucleic acids in cells by thermophilic strand displacement amplification
EP1252331B1 (en) Methods and compositions for detection of mycobacterium avium complex species
CN101415844B (zh) 通过扩增和检测核壳rna序列分析sars冠状病毒
JPH04505257A (ja) 核酸を主成分とする診断システムおよび血液製品中のニューモシスティスカリニの検出方法
MXPA97003730A (en) Detection of nucleic acids in cells by the method of amplification of the hebra by displacement, in a thermofil reaction
EP0550883A2 (en) Method and reagents for determining the presence of giardia lamblia
Nuovo 20 In Situ Amplification of cDNA
CN117230219A (zh) 一种检测结核分枝杆菌异烟肼耐药基因突变的核酸组合物、试剂盒及检测方法
EP1662966A2 (en) Assay for sars coronavirus by amplification and detection of the replicase sequence
JPH02174699A (ja) Hla型判別法及び試薬

Legal Events

Date Code Title Description
MK14 Patent ceased section 143(a) (annual fees not paid) or expired