WO1997011196A3 - Detection of nucleic acids in cells by thermophilic strand displacement amplification - Google Patents

Detection of nucleic acids in cells by thermophilic strand displacement amplification Download PDF

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Publication number
WO1997011196A3
WO1997011196A3 PCT/US1996/014648 US9614648W WO9711196A3 WO 1997011196 A3 WO1997011196 A3 WO 1997011196A3 US 9614648 W US9614648 W US 9614648W WO 9711196 A3 WO9711196 A3 WO 9711196A3
Authority
WO
WIPO (PCT)
Prior art keywords
cells
amplification
strand displacement
detection
nucleic acids
Prior art date
Application number
PCT/US1996/014648
Other languages
French (fr)
Other versions
WO1997011196A2 (en
Inventor
Kenton L Lohman
Natalie V Ostrerova
Cleve Mark Van
Robert Alan Reid
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US08/531,747 external-priority patent/US5631147A/en
Priority claimed from US08/531,749 external-priority patent/US5733752A/en
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Priority to AU70192/96A priority Critical patent/AU702896B2/en
Priority to BR9606653A priority patent/BR9606653A/en
Priority to EP96931542A priority patent/EP0796347A2/en
Priority to JP09512781A priority patent/JP3092163B2/en
Publication of WO1997011196A2 publication Critical patent/WO1997011196A2/en
Publication of WO1997011196A3 publication Critical patent/WO1997011196A3/en
Priority to MXPA/A/1997/003730A priority patent/MXPA97003730A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • C12Q1/701—Specific hybridization probes
    • C12Q1/702—Specific hybridization probes for retroviruses
    • C12Q1/703—Viruses associated with AIDS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Thermophilic Strand Displacement Amplification (tSDA) for amplification of nucleic acid target sequences in situ in cells in suspension, on slides or in tissues is described. Excellent specimen morphology is preserved, and either DNA targets, RNA targets, or both may be selectively amplified. In situ amplification by tSDA is compatible with immunochemical techniques, so that both amplification of target sequences and immunological staining can be performed on the same specimen.
PCT/US1996/014648 1995-09-21 1996-09-12 Detection of nucleic acids in cells by thermophilic strand displacement amplification WO1997011196A2 (en)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AU70192/96A AU702896B2 (en) 1995-09-21 1996-09-12 Detection of nucleic acids in cells by thermophilic strand displacement amplification
BR9606653A BR9606653A (en) 1995-09-21 1996-09-12 Detection of nucleic acids in cells by amplification of thermophilic tape displacement
EP96931542A EP0796347A2 (en) 1995-09-21 1996-09-12 Detection of nucleic acids in cells by thermophilic strand displacement amplification
JP09512781A JP3092163B2 (en) 1995-09-21 1996-09-12 Detection of nucleic acids in cells by thermophilic strand displacement amplification
MXPA/A/1997/003730A MXPA97003730A (en) 1995-09-21 1997-05-21 Detection of nucleic acids in cells by the method of amplification of the hebra by displacement, in a thermofil reaction

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US08/531,747 1995-09-21
US08/531,749 1995-09-21
US08/531,747 US5631147A (en) 1995-09-21 1995-09-21 Detection of nucleic acids in cells by thermophilic strand displacement amplification
US08/531,749 US5733752A (en) 1993-12-10 1995-09-21 Detection of nucleic acids in cells by thermophilic strand displacement amplification

Publications (2)

Publication Number Publication Date
WO1997011196A2 WO1997011196A2 (en) 1997-03-27
WO1997011196A3 true WO1997011196A3 (en) 1997-05-09

Family

ID=27063626

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/US1996/014648 WO1997011196A2 (en) 1995-09-21 1996-09-12 Detection of nucleic acids in cells by thermophilic strand displacement amplification

Country Status (6)

Country Link
EP (1) EP0796347A2 (en)
JP (2) JP3092163B2 (en)
AU (1) AU702896B2 (en)
BR (1) BR9606653A (en)
CA (1) CA2204641A1 (en)
WO (1) WO1997011196A2 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5916779A (en) * 1995-09-21 1999-06-29 Becton, Dickinson And Company Strand displacement amplification of RNA targets
US6187567B1 (en) * 1999-09-01 2001-02-13 Akzo Nobel N.V. Methods and reagents for in situ amplification
JP2002159295A (en) * 2000-11-27 2002-06-04 Keio Gijuku Method of quantitation of hiv-1 rna-dna hybrid and diagnostic kit
ATE319731T1 (en) * 2001-04-13 2006-03-15 Becton Dickinson Co AMPLIFICATION AND DETECTION OF MYCOPLASMA PNEUMONIAE
MXPA04000432A (en) 2001-07-15 2004-10-27 Keck Graduate Inst Amplification of nucleic acid fragments using nicking agents.
WO2003008624A2 (en) 2001-07-15 2003-01-30 Keck Graduate Institute Nucleic acid amplification using nicking agents
JP4903560B2 (en) * 2003-04-25 2012-03-28 ベクトン・ディキンソン・アンド・カンパニー Detection of herpes simplex virus type 1 and type 2 by nucleic acid amplification method
AU2005210362B8 (en) * 2004-02-09 2008-06-26 Fuso Pharmaceutical Industries, Ltd. Method of detecting nucleic acid and utilization thereof
FR2878254B1 (en) * 2004-11-22 2010-08-20 Bio Rad Pasteur COMPOSITION FOR AMPLIFICATION OF NUCLEIC ACIDS
CN102575220B (en) * 2009-09-03 2015-09-16 贝克顿·迪金森公司 For the method and composition of direct chemical cracking
CN112391450A (en) * 2020-11-19 2021-02-23 四川大学 High-specificity and high-sensitivity nucleic acid detection method based on modified dNTP

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0395398A2 (en) * 1989-04-27 1990-10-31 Life Technologies Inc. Amplification of nucleic acid sequences using random sequence oligonucleotides as primers
EP0459533A2 (en) * 1986-03-13 1991-12-04 F. Hoffmann-La Roche Ag Probe groups for the detection of nucleotide variations and genetic polymorphisms
WO1993002215A1 (en) * 1991-07-24 1993-02-04 Royal Free Hospital School Of Medicine Quantitative nucleic acid amplification
EP0543612A2 (en) * 1991-11-19 1993-05-26 Becton, Dickinson and Company Nucleic acid target generation
EP0617132A2 (en) * 1993-03-26 1994-09-28 Gen-Probe Incorporated Probes and method to detect human immunodeficiency virus type 1
EP0657548A1 (en) * 1993-12-10 1995-06-14 Becton, Dickinson and Company Amplification of nucleic acids in cells
WO1995025180A1 (en) * 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0459533A2 (en) * 1986-03-13 1991-12-04 F. Hoffmann-La Roche Ag Probe groups for the detection of nucleotide variations and genetic polymorphisms
EP0395398A2 (en) * 1989-04-27 1990-10-31 Life Technologies Inc. Amplification of nucleic acid sequences using random sequence oligonucleotides as primers
WO1993002215A1 (en) * 1991-07-24 1993-02-04 Royal Free Hospital School Of Medicine Quantitative nucleic acid amplification
EP0543612A2 (en) * 1991-11-19 1993-05-26 Becton, Dickinson and Company Nucleic acid target generation
EP0617132A2 (en) * 1993-03-26 1994-09-28 Gen-Probe Incorporated Probes and method to detect human immunodeficiency virus type 1
EP0657548A1 (en) * 1993-12-10 1995-06-14 Becton, Dickinson and Company Amplification of nucleic acids in cells
WO1995025180A1 (en) * 1994-03-16 1995-09-21 Gen-Probe Incorporated Isothermal strand displacement nucleic acid amplification

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
FRAISER M ET AL: "Single-genome detection of M. tuberculosis by thermophilic strand displacement amplification", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY FOR MICROBIOLOGY, vol. 95, no. 0, 21 May 1995 (1995-05-21), pages 131, XP000613514 *
WRIGHT D ET AL: "thermophilic strand displacement amplification. A rapid method for amplification of DNA sequences to extreme high copy number", ABSTRACTS OF THE GENERAL MEETING OF THE AMERICAN SOCIETY OF MICROBIOLOGY, vol. 95, no. 0, 21 May 1995 (1995-05-21), pages 133, XP000613515 *

Also Published As

Publication number Publication date
BR9606653A (en) 1997-11-04
JP2000300281A (en) 2000-10-31
WO1997011196A2 (en) 1997-03-27
AU702896B2 (en) 1999-03-11
EP0796347A2 (en) 1997-09-24
MX9703730A (en) 1997-09-30
JP3092163B2 (en) 2000-09-25
AU7019296A (en) 1997-04-09
CA2204641A1 (en) 1997-03-27
JPH10500313A (en) 1998-01-13

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