AU689569B2 - Enhanced secretion of polypeptides - Google Patents

Enhanced secretion of polypeptides Download PDF

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Publication number
AU689569B2
AU689569B2 AU21198/95A AU2119895A AU689569B2 AU 689569 B2 AU689569 B2 AU 689569B2 AU 21198/95 A AU21198/95 A AU 21198/95A AU 2119895 A AU2119895 A AU 2119895A AU 689569 B2 AU689569 B2 AU 689569B2
Authority
AU
Australia
Prior art keywords
nucleic acid
polypeptide
sequence
seq
ngf
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU21198/95A
Other languages
English (en)
Other versions
AU2119895A (en
Inventor
Shi-Yuan Meng
Charles F. Morris
Larry B. Tsai
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Amgen Inc
Original Assignee
Amgen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amgen Inc filed Critical Amgen Inc
Publication of AU2119895A publication Critical patent/AU2119895A/en
Application granted granted Critical
Publication of AU689569B2 publication Critical patent/AU689569B2/en
Anticipated expiration legal-status Critical
Ceased legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/48—Nerve growth factor [NGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/035—Fusion polypeptide containing a localisation/targetting motif containing a signal for targeting to the external surface of a cell, e.g. to the outer membrane of Gram negative bacteria, GPI- anchored eukaryote proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
AU21198/95A 1994-03-18 1995-03-16 Enhanced secretion of polypeptides Ceased AU689569B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US215138 1994-03-18
US08/215,138 US5470719A (en) 1994-03-18 1994-03-18 Modified OmpA signal sequence for enhanced secretion of polypeptides
PCT/US1995/003175 WO1995025743A1 (fr) 1994-03-18 1995-03-16 Secretion amelioree de polypeptides

Publications (2)

Publication Number Publication Date
AU2119895A AU2119895A (en) 1995-10-09
AU689569B2 true AU689569B2 (en) 1998-04-02

Family

ID=22801818

Family Applications (1)

Application Number Title Priority Date Filing Date
AU21198/95A Ceased AU689569B2 (en) 1994-03-18 1995-03-16 Enhanced secretion of polypeptides

Country Status (15)

Country Link
US (2) US5470719A (fr)
EP (1) EP0750634A1 (fr)
JP (1) JPH09510611A (fr)
AU (1) AU689569B2 (fr)
CA (1) CA2185343A1 (fr)
CZ (1) CZ284204B6 (fr)
FI (1) FI963655A (fr)
HU (1) HUT76674A (fr)
IL (1) IL112982A0 (fr)
MX (1) MX9604047A (fr)
NO (1) NO963818L (fr)
NZ (1) NZ283214A (fr)
SK (1) SK116896A3 (fr)
WO (1) WO1995025743A1 (fr)
ZA (1) ZA952218B (fr)

Families Citing this family (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5747662A (en) * 1995-03-01 1998-05-05 Genentech, Inc. Methods and compositions for secretion of heterologous polypeptides
US5888796A (en) * 1996-01-31 1999-03-30 St. Louis University Clone of a nucleotide sequence encoding a protein having two functions
US20020065394A1 (en) * 1998-03-18 2002-05-30 Kenneth Jacobs Secreted proteins and polynucleotides encoding them
KR100360594B1 (ko) * 2000-01-19 2002-11-13 한미약품공업 주식회사 인간 인터페론 알파의 발현 분비벡터 및 이를 이용한인터페론 알파의 생산 방법
JP2004510410A (ja) 2000-06-05 2004-04-08 コリクサ　コーポレイション 宿主細胞由来組換えタンパク質の分泌を促進するリーダーペプチド
US20060239966A1 (en) * 2003-10-20 2006-10-26 Tornoee Jens In vivo gene therapy of parkinson's disease
US20100196336A1 (en) 2006-05-23 2010-08-05 Dongsu Park Modified dendritic cells having enhanced survival and immunogenicity and related compositions and methods
US8097422B2 (en) 2007-06-20 2012-01-17 Salk Institute For Biological Studies Kir channel modulators
US20090280103A1 (en) * 2008-04-04 2009-11-12 Martin Flueck Regulation of muscle repair
CA2744523A1 (fr) * 2008-09-22 2010-03-25 Calmune Corporation Procedes et vecteurs de presentation de molecules et molecules presentees et collections
CA2765849A1 (fr) 2009-07-02 2011-01-06 Verdezyne, Inc. Procedes biologiques pour preparer de l'acide adipique
US20120184007A1 (en) * 2009-07-09 2012-07-19 Stephen Picataggio Engineered microorganisms with enhanced fermentation activity
US8889394B2 (en) * 2009-09-07 2014-11-18 Empire Technology Development Llc Multiple domain proteins
US8728798B2 (en) 2011-05-03 2014-05-20 Verdezyne, Inc. Biological methods for preparing adipic acid
US8343752B2 (en) 2011-05-03 2013-01-01 Verdezyne, Inc. Biological methods for preparing adipic acid
MY165893A (en) 2011-07-06 2018-05-18 Verdezyne Inc Biological methods for preparing a fatty dicarboxylic acid
WO2014100504A2 (fr) 2012-12-19 2014-06-26 Verdezyne, Inc. Procédés biologiques de préparation d'un acide gras dicarboxylique
EP2935563B1 (fr) 2012-12-19 2020-11-04 Corvay Bioproducts GmbH Procédés biologiques pour la préparation d'un acide dicarboxylique gras
US10513706B2 (en) 2014-04-09 2019-12-24 The Scripps Research Institute Import of unnatural or modified nucleoside triphosphates into cells via nucleic acid triphosphate transporters
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
WO2016154046A2 (fr) 2015-03-20 2016-09-29 Verdezyne, Inc. Procédés biologiques pour préparer de l'acide 3-hydroxypropionique
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
AU2018300069A1 (en) 2017-07-11 2020-02-27 Synthorx, Inc. Incorporation of unnatural nucleotides and methods thereof
US11174488B2 (en) 2017-07-13 2021-11-16 Radici Chimica S.P.A. Biological methods for modifying cellular carbon flux
US20200181220A1 (en) 2017-08-03 2020-06-11 Synthorx, Inc. Cytokine conjugates for the treatment of proliferative and infectious diseases
CN108610398B (zh) * 2018-01-15 2020-08-11 武汉海特生物制药股份有限公司 一段功能序列及在分泌蛋白表达中的应用
WO2020060948A1 (fr) 2018-09-17 2020-03-26 Levadura Biotechnology, Inc. Production de cannabinoïdes dans une levure à l'aide d'une charge d'alimentation d'acides gras
CN114949240A (zh) 2019-02-06 2022-08-30 新索思股份有限公司 Il-2缀合物及其使用方法
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992005254A1 (fr) * 1990-09-25 1992-04-02 Genentech, Inc. Nouveau facteur neurotrophique
WO1993008300A1 (fr) * 1991-10-18 1993-04-29 The University Of Calgary Vecteurs d'expression/secretion destines a la production de fragments de fv biologiquement actifs

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4338397A (en) * 1980-04-11 1982-07-06 President And Fellows Of Harvard College Mature protein synthesis
US4757013A (en) * 1983-07-25 1988-07-12 The Research Foundation Of State University Of New York Cloning vehicles for polypeptide expression in microbial hosts
US5235043A (en) * 1990-04-06 1993-08-10 Synergen, Inc. Production of biologically active, recombinant members of the ngf/bdnf family of neurotrophic proteins
JPH07509600A (ja) * 1992-06-12 1995-10-26 リジェネロン　ファーマシューティカルズ，インコーポレーテッド ニューロトロフィン−４発現に基づく治療および診断法
US5474914A (en) * 1992-07-29 1995-12-12 Chiron Corporation Method of producing secreted CMV glycoprotein H

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992005254A1 (fr) * 1990-09-25 1992-04-02 Genentech, Inc. Nouveau facteur neurotrophique
WO1993008300A1 (fr) * 1991-10-18 1993-04-29 The University Of Calgary Vecteurs d'expression/secretion destines a la production de fragments de fv biologiquement actifs

Also Published As

Publication number Publication date
MX9604047A (es) 1997-09-30
IL112982A0 (en) 1995-06-29
ZA952218B (en) 1995-12-12
SK116896A3 (en) 1997-08-06
JPH09510611A (ja) 1997-10-28
HUT76674A (en) 1997-10-28
CZ284204B6 (cs) 1998-09-16
AU2119895A (en) 1995-10-09
US5608036A (en) 1997-03-04
NZ283214A (en) 1998-03-25
HU9602548D0 (en) 1996-11-28
CA2185343A1 (fr) 1995-09-28
CZ264496A3 (en) 1997-05-14
NO963818L (no) 1996-11-15
EP0750634A1 (fr) 1997-01-02
US5470719A (en) 1995-11-28
NO963818D0 (no) 1996-09-12
FI963655A (fi) 1996-11-15
WO1995025743A1 (fr) 1995-09-28
FI963655A0 (fi) 1996-09-16

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Date Code Title Description
MK14 Patent ceased section 143(a) (annual fees not paid) or expired