AU2016369071B2 - Modified nanopores, compositions comprising the same, and uses thereof - Google Patents

Modified nanopores, compositions comprising the same, and uses thereof Download PDF

Info

Publication number
AU2016369071B2
AU2016369071B2 AU2016369071A AU2016369071A AU2016369071B2 AU 2016369071 B2 AU2016369071 B2 AU 2016369071B2 AU 2016369071 A AU2016369071 A AU 2016369071A AU 2016369071 A AU2016369071 A AU 2016369071A AU 2016369071 B2 AU2016369071 B2 AU 2016369071B2
Authority
AU
Australia
Prior art keywords
nanopore
clya
amino acid
dna
polynucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2016369071A
Other languages
English (en)
Other versions
AU2016369071A1 (en
Inventor
Tine BROUNS
Lorenzo FRANCESCHINI
Andrew John Heron
Lakmal Nishantha JAYASINGHE
Giovanni Maglia
Elizabeth Jayne Wallace
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Katholieke Universiteit Leuven
Original Assignee
Katholieke Universiteit Leuven
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Katholieke Universiteit Leuven filed Critical Katholieke Universiteit Leuven
Publication of AU2016369071A1 publication Critical patent/AU2016369071A1/en
Application granted granted Critical
Publication of AU2016369071B2 publication Critical patent/AU2016369071B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/001—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof by chemical synthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Physics & Mathematics (AREA)
  • Nanotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2016369071A 2015-12-08 2016-12-08 Modified nanopores, compositions comprising the same, and uses thereof Active AU2016369071B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562264709P 2015-12-08 2015-12-08
US62/264,709 2015-12-08
PCT/IB2016/001841 WO2017098322A1 (en) 2015-12-08 2016-12-08 Modified nanopores, compositions comprising the same, and uses thereof

Publications (2)

Publication Number Publication Date
AU2016369071A1 AU2016369071A1 (en) 2018-06-28
AU2016369071B2 true AU2016369071B2 (en) 2022-05-19

Family

ID=58098655

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2016369071A Active AU2016369071B2 (en) 2015-12-08 2016-12-08 Modified nanopores, compositions comprising the same, and uses thereof

Country Status (8)

Country Link
US (1) US10976300B2 (cg-RX-API-DMAC7.html)
EP (1) EP3387432B1 (cg-RX-API-DMAC7.html)
JP (2) JP7237586B2 (cg-RX-API-DMAC7.html)
KR (1) KR102837999B1 (cg-RX-API-DMAC7.html)
CN (1) CN109072295B (cg-RX-API-DMAC7.html)
AU (1) AU2016369071B2 (cg-RX-API-DMAC7.html)
CA (1) CA3011830C (cg-RX-API-DMAC7.html)
WO (1) WO2017098322A1 (cg-RX-API-DMAC7.html)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
CN117164683A (zh) 2014-09-01 2023-12-05 弗拉芒区生物技术研究所 突变csgg孔
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
KR102222192B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
EP4397970A3 (en) 2016-04-06 2024-10-09 Oxford Nanopore Technologies PLC Mutant pore
WO2018136495A1 (en) * 2017-01-18 2018-07-26 Youhealth Biotech, Limited Methods and compositions for nanopore sequencing
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
CN119192308A (zh) 2017-06-30 2024-12-27 弗拉芒区生物技术研究所 新颖蛋白孔
WO2020010589A1 (en) * 2018-07-12 2020-01-16 Shenzhen Genorivision Technology Co., Ltd. An apparatus for biopolymer sequencing
WO2020241752A1 (en) 2019-05-28 2020-12-03 The University Of Tokyo Analyzing apparatus and method using a pore device
IL293024A (en) * 2019-11-19 2022-07-01 Univ Groningen Artificial fertility, methods and uses thereof
JP2023503514A (ja) * 2019-12-02 2023-01-30 オックスフォード ナノポール テクノロジーズ ピーエルシー ナノポアを使用する標的ペプチドの特徴付けの方法
NL2024579B1 (en) * 2019-12-24 2021-09-06 Univ Delft Tech Protein and peptide fingerprinting and sequencing by nanopore translocation of peptide-oligonucleotide complexes
CN115398008B (zh) * 2020-04-14 2025-05-09 成都今是科技有限公司 纳米孔制备和检测方法及其检测装置
CN112179955B (zh) * 2020-09-27 2024-05-10 西北工业大学 基于聚精氨酸和聚谷氨酸逐层修饰的纳米孔制备pH响应的纳米流体二极管方法
US20240377380A1 (en) * 2021-03-31 2024-11-14 Illumina, Inc. Nanopore sensor devices
GB2610380A (en) 2021-08-23 2023-03-08 Cambridge Entpr Ltd Nucleic acid detection
GB202113935D0 (en) 2021-09-29 2021-11-10 Cambridge Entpr Ltd Nucleic acid characterisation
GB202118906D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
GB202118908D0 (en) 2021-12-23 2022-02-09 Oxford Nanopore Tech Ltd Method
CN114768530B (zh) * 2022-04-29 2023-03-28 中国工程物理研究院材料研究所 二硫化钼在电解分离氢同位素中的应用
GB202216162D0 (en) 2022-10-31 2022-12-14 Oxford Nanopore Tech Plc Method
GB202307486D0 (en) 2023-05-18 2023-07-05 Oxford Nanopore Tech Plc Method
GB202317028D0 (en) 2023-11-06 2023-12-20 Oxford Nanopore Tech Ltd Method

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015166276A1 (en) * 2014-05-02 2015-11-05 Oxford Nanopore Technologies Limited Method of improving the movement of a target polynucleotide with respect to a transmembrane pore

Family Cites Families (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5795782A (en) 1995-03-17 1998-08-18 President & Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
JP3891620B2 (ja) 1996-11-14 2007-03-14 株式会社アイシン・コスモス研究所 ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法
US20020197614A1 (en) 1997-05-16 2002-12-26 Mosaic Technologies, Inc. Electrophoretic analysis of target molecules using adapter molecules
AU8586298A (en) 1997-07-25 1999-02-16 University Of Massachusetts Designed protein pores as components for biosensors
US6743605B1 (en) 1998-06-24 2004-06-01 Enzo Life Sciences, Inc. Linear amplification of specific nucleic acid sequences
US6267872B1 (en) 1998-11-06 2001-07-31 The Regents Of The University Of California Miniature support for thin films containing single channels or nanopores and methods for using same
US6426231B1 (en) 1998-11-18 2002-07-30 The Texas A&M University System Analyte sensing mediated by adapter/carrier molecules
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
EP1192103A1 (en) 1999-06-22 2002-04-03 President And Fellows of Harvard College Control of solid state dimensional features
WO2001016327A2 (de) 1999-08-31 2001-03-08 Michael Niederweis Verfahren zur herstellung eines kanalbildenden proteins
AU2001241474A1 (en) 2000-02-11 2001-08-20 The Texas A And M University System Biosensor compositions and methods of use
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
WO2002042496A2 (en) 2000-11-27 2002-05-30 The Regents Of The University Of California Methods and devices for characterizing duplex nucleic acid molecules
US6863833B1 (en) 2001-06-29 2005-03-08 The Board Of Trustees Of The Leland Stanford Junior University Microfabricated apertures for supporting bilayer lipid membranes
WO2003095669A1 (en) 2002-05-10 2003-11-20 The Texas A & M University System Stochastic sensing through covalent interactions
CA2519309A1 (en) 2003-03-25 2004-10-14 Stratagene California Dna polymerase fusions and uses thereof
US7163658B2 (en) 2003-04-23 2007-01-16 Rouvain Bension Rapid sequencing of polymers
JP2005253427A (ja) 2004-03-15 2005-09-22 Aisin Seiki Co Ltd 核酸検出方法及び核酸単離方法
WO2006028508A2 (en) 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
WO2005124888A1 (en) 2004-06-08 2005-12-29 President And Fellows Of Harvard College Suspended carbon nanotube field effect transistor
US20060105461A1 (en) 2004-10-22 2006-05-18 May Tom-Moy Nanopore analysis system
WO2007084103A2 (en) 2004-12-21 2007-07-26 The Texas A & M University System High temperature ion channels and pores
GB0505971D0 (en) 2005-03-23 2005-04-27 Isis Innovation Delivery of molecules to a lipid bilayer
KR100730350B1 (ko) 2005-10-17 2007-06-19 삼성전자주식회사 표면처리된 나노포어를 이용한 ｄｎａ 검출방법 및검출장치
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
EP1963530B1 (en) 2005-12-22 2011-07-27 Pacific Biosciences of California, Inc. Active surface coupled polymerases
US7638034B2 (en) 2006-09-21 2009-12-29 Los Alamos National Security, Llc Electrochemical detection of single molecules using abiotic nanopores having electrically tunable dimensions
AU2008217578A1 (en) 2007-02-20 2008-08-28 Oxford Nanopore Technologies Limited Lipid bilayer sensor system
EP3798317B1 (en) 2007-04-04 2024-01-03 The Regents of the University of California Compositions, devices, systems, and methods for using a nanopore
GB0716264D0 (en) 2007-08-21 2007-09-26 Isis Innovation Bilayers
WO2009035647A1 (en) 2007-09-12 2009-03-19 President And Fellows Of Harvard College High-resolution molecular graphene sensor comprising an aperture in the graphene layer
GB2453377A (en) 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
GB0724736D0 (en) 2007-12-19 2008-01-30 Oxford Nanolabs Ltd Formation of layers of amphiphilic molecules
US8231969B2 (en) 2008-03-26 2012-07-31 University Of Utah Research Foundation Asymmetrically functionalized nanoparticles
WO2009120372A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
US8772041B2 (en) 2008-05-22 2014-07-08 The Regents Of The University Of California Membrane precursors and membranes formed therefrom
US8652771B2 (en) 2008-05-28 2014-02-18 University of Souther California Measurement of succinate in urine samples as a biomarker of kidney damage in diabetic subjects
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
WO2010004273A1 (en) 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
CN102216783B (zh) 2008-09-22 2015-04-01 华盛顿大学 Msp纳米微孔和相关方法
US9080211B2 (en) 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
WO2010062903A2 (en) * 2008-11-26 2010-06-03 Board Of Regents, The University Of Texas System Genomic sequencing using modified protein pores and ionic liquids
KR101797773B1 (ko) 2009-01-30 2017-11-15 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 시퀀싱에서 핵산 구축물용 어댑터
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
CA2758617C (en) 2009-04-20 2017-10-03 Oxford Nanopore Technologies Limited Lipid bilayer sensor array
BR112012013074B1 (pt) 2009-12-01 2018-09-18 Oxford Nanopore Technologies Limited instrumento de análise e módulo para realizar análise bioquímica, e, método para operar um instrumento de análise para realizar análise bioquímica
EP2539707B1 (en) 2010-02-23 2021-06-30 University Of Washington Artificial mycolic acid membranes
WO2011125015A2 (en) * 2010-04-05 2011-10-13 Bar-Ilan University Protease-activatable pore-forming polypeptides
US9593370B2 (en) 2010-10-01 2017-03-14 Oxford Nanopore Technologies Ltd. Biochemical analysis apparatus and rotary valve
CN102116783B (zh) 2010-12-31 2013-05-29 北京普源精电科技有限公司 一种波形显示方法
AU2012215135B9 (en) 2011-02-11 2017-03-09 Oxford Nanopore Technologies Limited Mutant pores
EP4273092A3 (en) 2011-05-27 2024-01-17 Oxford Nanopore Technologies plc Method and apparatus for determining the presence, absence or characteristics of an analyte
WO2012166906A1 (en) 2011-05-31 2012-12-06 Massachusetts Institute Of Technology Cell-directed synthesis of multifunctional nanopatterns and nanomaterials
CA2843136C (en) 2011-07-25 2020-12-29 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
CA2849624C (en) 2011-09-23 2021-05-25 Oxford Nanopore Technologies Limited Analysis of a polymer comprising polymer units
EP2768977B1 (en) 2011-10-21 2015-09-23 Oxford Nanopore Technologies Limited Method of characterizing a target polynucleotide using a pore and a hel308 helicase
WO2013098562A2 (en) 2011-12-29 2013-07-04 Oxford Nanopore Technologies Limited Enzyme method
EP2798083B1 (en) 2011-12-29 2017-08-09 Oxford Nanopore Technologies Limited Method for characterising a polynucelotide by using a xpd helicase
EP2807476A4 (en) 2012-01-20 2015-12-09 Genia Technologies Inc DETECTION AND SEQUENCING OF MOLECULES ON NANOPORENE BASE
JP6275052B2 (ja) 2012-02-15 2018-02-07 オックスフォード ナノポール テクノロジーズ リミテッド アプタマー法
KR102083695B1 (ko) 2012-04-10 2020-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 리세닌 기공
TWI655213B (zh) 2012-07-13 2019-04-01 目立康股份有限公司 自我組織化肽衍生物的製造方法
JP6429773B2 (ja) 2012-07-19 2018-11-28 オックスフォード ナノポール テクノロジーズ リミテッド 酵素構築物
EP2875128B8 (en) 2012-07-19 2020-06-24 Oxford Nanopore Technologies Limited Modified helicases
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
EP2911771B1 (en) 2012-10-26 2017-01-18 Oxford Nanopore Technologies Limited Droplet interfaces
GB201313121D0 (en) 2013-07-23 2013-09-04 Oxford Nanopore Tech Ltd Array of volumes of polar medium
CN105074458B (zh) 2013-02-07 2019-06-28 耶路撒冷希伯来大学伊森姆研究发展有限公司 杂化纳米孔及其用于检测分析物的用途
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
SG11201507138RA (en) 2013-03-08 2015-10-29 Oxford Nanopore Tech Ltd Enzyme stalling method
WO2014153047A1 (en) 2013-03-14 2014-09-25 The Trustees Of Boston University Optoelectronic control of solid-state nanopores
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
WO2015051378A1 (en) * 2013-10-04 2015-04-09 University Of Washington Through Its Center For Commercialization Systems and methods for nanopore-based analysis of nucleic acids
GB201406151D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
JP6677640B2 (ja) 2013-10-18 2020-04-08 オックスフォード ナノポール テクノロジーズ リミテッド 修飾酵素
GB201406155D0 (en) 2014-04-04 2014-05-21 Oxford Nanopore Tech Ltd Method
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10337060B2 (en) 2014-04-04 2019-07-02 Oxford Nanopore Technologies Ltd. Method for characterising a double stranded nucleic acid using a nano-pore and anchor molecules at both ends of said nucleic acid
FR3023394B1 (fr) 2014-07-02 2017-12-29 Adn Access Data Networks Dispositif permettant de faciliter l'enseignement de la langue amharique et d'en normaliser l'ecriture
CN117164683A (zh) 2014-09-01 2023-12-05 弗拉芒区生物技术研究所 突变csgg孔
EP3204511B1 (en) 2014-10-07 2021-07-28 Oxford Nanopore Technologies Limited Mutant pores
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
US11169138B2 (en) 2015-04-14 2021-11-09 Katholieke Universiteit Leuven Nanopores with internal protein adaptors
KR102222192B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015166276A1 (en) * 2014-05-02 2015-11-05 Oxford Nanopore Technologies Limited Method of improving the movement of a target polynucleotide with respect to a transmembrane pore

Also Published As

Publication number Publication date
CA3011830C (en) 2024-11-12
KR102837999B1 (ko) 2025-07-24
CN109072295A (zh) 2018-12-21
CA3011830A1 (en) 2017-06-15
JP2019507583A (ja) 2019-03-22
AU2016369071A1 (en) 2018-06-28
KR20180089499A (ko) 2018-08-08
EP3387432B1 (en) 2022-09-28
JP7237586B2 (ja) 2023-03-13
EP3387432A1 (en) 2018-10-17
US10976300B2 (en) 2021-04-13
CN109072295B (zh) 2022-05-31
WO2017098322A1 (en) 2017-06-15
JP2022046503A (ja) 2022-03-23
US20180364214A1 (en) 2018-12-20

Similar Documents

Publication Publication Date Title
AU2016369071B2 (en) Modified nanopores, compositions comprising the same, and uses thereof
US20240060126A1 (en) Method
EP3619224B1 (en) Transmembrane pore consisting of two csgg pores
KR102086182B1 (ko) 효소 방법
CN107002151B (zh) 向跨膜孔输送分析物的方法
EP3204511B1 (en) Mutant pores
US9551023B2 (en) Sample preparation method
CN106232834A (zh) 样品制备方法
EP3259281A1 (en) Hetero-pores

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)