AU2015236008B2 - Methods of preparing a poloxamer for use in cell culture medium - Google Patents

Methods of preparing a poloxamer for use in cell culture medium Download PDF

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Publication number
AU2015236008B2
AU2015236008B2 AU2015236008A AU2015236008A AU2015236008B2 AU 2015236008 B2 AU2015236008 B2 AU 2015236008B2 AU 2015236008 A AU2015236008 A AU 2015236008A AU 2015236008 A AU2015236008 A AU 2015236008A AU 2015236008 B2 AU2015236008 B2 AU 2015236008B2
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Australia
Prior art keywords
poloxamer
minutes
cell
cell culture
heated
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AU2015236008A
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AU2015236008A1 (en
Inventor
Amelia ADAMS
Kara Calhoun
Alan Deese
Marion GLENN
Robert David Kiss
Stefan Koenig
Inchan Kwon
Steven J. Meier
Ganesh Prasadh Vissvesvaran
Kate WINCHESTER
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Genentech Inc
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Genentech Inc
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/02—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length in solution
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/02—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from cyclic ethers by opening of the heterocyclic ring
    • C08G65/30—Post-polymerisation treatment, e.g. recovery, purification, drying
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/34—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from hydroxy compounds or their metallic derivatives
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G65/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G65/34—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule from hydroxy compounds or their metallic derivatives
    • C08G65/48—Polymers modified by chemical after-treatment
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G2650/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G2650/28—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the polymer type
    • C08G2650/50—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the polymer type containing nitrogen, e.g. polyetheramines or Jeffamines(r)
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
    • C08G2650/00—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule
    • C08G2650/28—Macromolecular compounds obtained by reactions forming an ether link in the main chain of the macromolecule characterised by the polymer type
    • C08G2650/58—Ethylene oxide or propylene oxide copolymers, e.g. pluronics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/50—Soluble polymers, e.g. polyethyleneglycol [PEG]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Polyesters Or Polycarbonates (AREA)
  • Compositions Of Macromolecular Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2015236008A 2014-03-25 2015-03-25 Methods of preparing a poloxamer for use in cell culture medium Active AU2015236008B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461970281P 2014-03-25 2014-03-25
US61/970,281 2014-03-25
PCT/US2015/022592 WO2015148736A1 (en) 2014-03-25 2015-03-25 Methods of preparing a poloxamer for use in cell culture medium

Publications (2)

Publication Number Publication Date
AU2015236008A1 AU2015236008A1 (en) 2016-10-27
AU2015236008B2 true AU2015236008B2 (en) 2018-12-20

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AU2015236008A Active AU2015236008B2 (en) 2014-03-25 2015-03-25 Methods of preparing a poloxamer for use in cell culture medium

Country Status (19)

Country Link
US (2) US11034793B2 (enExample)
EP (2) EP3778702B1 (enExample)
JP (3) JP6655548B2 (enExample)
KR (1) KR102352250B1 (enExample)
CN (2) CN111808276A (enExample)
AU (1) AU2015236008B2 (enExample)
BR (1) BR112016021739B1 (enExample)
CA (1) CA2943357C (enExample)
ES (1) ES2778680T3 (enExample)
HR (1) HRP20200372T1 (enExample)
IL (2) IL247902B (enExample)
MX (2) MX2016012288A (enExample)
MY (1) MY178060A (enExample)
PL (1) PL3122799T3 (enExample)
RU (1) RU2710551C2 (enExample)
SG (1) SG11201607929PA (enExample)
SI (1) SI3122799T1 (enExample)
WO (1) WO2015148736A1 (enExample)
ZA (1) ZA201606711B (enExample)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201607929PA (en) 2014-03-25 2016-10-28 Genentech Inc Methods of preparing a poloxamer for use in cell culture medium
KR20180125999A (ko) 2016-03-17 2018-11-26 메르크 파텐트 게엠베하 폴록사머의 정제 방법
KR102544922B1 (ko) * 2017-12-21 2023-06-16 시그마-알드리치 컴퍼니., 엘엘씨 폴록사머 조성물 및 이의 제조 및 사용 방법
WO2020013725A1 (en) * 2018-07-12 2020-01-16 Arshintseva Elena Valentinovna Thermal method for sterilizing poloxamer comprising liquid drugs
JPWO2020022511A1 (ja) * 2018-07-27 2021-08-02 味の素株式会社 動物細胞の浮遊培養用の添加物、浮遊培養用培地および浮遊培養方法

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EP1661558B1 (en) * 2004-11-30 2010-07-21 Basf Se Compositions based on micro-prilled poloxamer particles and methods for their manufacturing

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Also Published As

Publication number Publication date
CN111808276A (zh) 2020-10-23
ZA201606711B (en) 2019-12-18
EP3778702A1 (en) 2021-02-17
AU2015236008A1 (en) 2016-10-27
KR20160138161A (ko) 2016-12-02
RU2710551C2 (ru) 2019-12-27
EP3122799A1 (en) 2017-02-01
IL279583A (en) 2021-03-01
HRP20200372T1 (hr) 2020-06-12
RU2016141568A (ru) 2018-04-28
EP3122799B8 (en) 2020-03-11
EP3778702B1 (en) 2023-06-28
MX2021001981A (es) 2021-04-28
RU2016141568A3 (enExample) 2018-09-26
MY178060A (en) 2020-09-30
SI3122799T1 (sl) 2020-06-30
JP2023011566A (ja) 2023-01-24
EP3122799B1 (en) 2020-01-15
US20210371587A1 (en) 2021-12-02
JP6655548B2 (ja) 2020-02-26
WO2015148736A8 (en) 2016-08-18
MX2016012288A (es) 2017-01-23
IL247902B (en) 2021-01-31
IL247902A0 (en) 2016-11-30
WO2015148736A1 (en) 2015-10-01
BR112016021739A2 (pt) 2017-08-15
US11912823B2 (en) 2024-02-27
CN106414552A (zh) 2017-02-15
JP2020103290A (ja) 2020-07-09
KR102352250B1 (ko) 2022-01-17
ES2778680T3 (es) 2020-08-11
JP7472222B2 (ja) 2024-04-22
PL3122799T3 (pl) 2020-06-29
IL279583B (en) 2022-03-01
US11034793B2 (en) 2021-06-15
SG11201607929PA (en) 2016-10-28
US20170009015A1 (en) 2017-01-12
NZ725151A (en) 2021-05-28
CN106414552B (zh) 2020-08-25
JP2017516459A (ja) 2017-06-22
CA2943357C (en) 2023-06-27
BR112016021739B1 (pt) 2021-11-03
CA2943357A1 (en) 2015-10-01

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