AU2015223025B2 - Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes - Google Patents

Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes Download PDF

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AU2015223025B2
AU2015223025B2 AU2015223025A AU2015223025A AU2015223025B2 AU 2015223025 B2 AU2015223025 B2 AU 2015223025B2 AU 2015223025 A AU2015223025 A AU 2015223025A AU 2015223025 A AU2015223025 A AU 2015223025A AU 2015223025 B2 AU2015223025 B2 AU 2015223025B2
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AU2015223025A1 (en
Inventor
Robert Dicosimo
Erwin Columbus HAGO
Susan Marie Hennessey
Ling Hua
Kevin D. Nagy
Rodrigo Ramirez
Jayarama K. Shetty
Zhongmei TANG
Zheyong YU
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Nutrition and Biosciences USA 4 Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01005—Dextransucrase (2.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01024—1,4-Alpha-glucan 6-alpha-glucosyltransferase (2.4.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0107—Glucan 1,6-alpha-glucosidase (3.2.1.70)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01084—Glucan 1,3-alpha-glucosidase (3.2.1.84), i.e. mutanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01115—Branched-dextran exo-1,2-alpha-glucosidase (3.2.1.115)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2015223025A 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes Active AU2015223025B2 (en)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US201461945241P 2014-02-27 2014-02-27
US201461945233P 2014-02-27 2014-02-27
US61/945,241 2014-02-27
US61/945,233 2014-02-27
US201462004314P 2014-05-29 2014-05-29
US201462004312P 2014-05-29 2014-05-29
US201462004300P 2014-05-29 2014-05-29
US201462004290P 2014-05-29 2014-05-29
US201462004308P 2014-05-29 2014-05-29
US201462004305P 2014-05-29 2014-05-29
US62/004,308 2014-05-29
US62/004,300 2014-05-29
US62/004,312 2014-05-29
US62/004,290 2014-05-29
US62/004,314 2014-05-29
US62/004,305 2014-05-29
CNPCT/CN2015/073269 2015-02-25
CN2015073269 2015-02-25
PCT/US2015/017644 WO2015130881A1 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

Publications (2)

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AU2015223025A1 AU2015223025A1 (en) 2016-08-04
AU2015223025B2 true AU2015223025B2 (en) 2018-09-20

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AU2015223025A Active AU2015223025B2 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
AU2015223027A Active AU2015223027B2 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

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EP (2) EP3110959A1 (https=)
JP (2) JP6929499B2 (https=)
CN (2) CN106460023B (https=)
AU (2) AU2015223025B2 (https=)
BR (1) BR112016019823B1 (https=)
CA (2) CA2940778A1 (https=)
WO (2) WO2015130883A1 (https=)

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BR112017013368B1 (pt) 2014-12-22 2021-11-23 Nutrition & Biosciences USA 4, Inc. Composição de mistura polimérica
US11351104B2 (en) 2015-02-06 2022-06-07 Nutrition & Biosciences USA 4, Inc. Colloidal dispersions of poly alpha-1,3-glucan based polymers
US10294466B2 (en) 2015-02-25 2019-05-21 Danisco Us Inc. Alpha-glucosidase, compositions and methods
ES2827832T3 (es) 2015-06-01 2021-05-24 Dupont Ind Biosciences Usa Llc Fíbridos de poli alfa-1,3-glucano y usos de los mismos y procesos para hacer fíbridos de poli alfa-1,3-glucano
US10738266B2 (en) 2015-06-01 2020-08-11 Dupont Industrial Biosciences Usa, Llc Structured liquid compositions comprising colloidal dispersions of poly alpha-1,3-glucan
CA2997563C (en) 2015-10-26 2022-03-22 E. I. Du Pont De Nemours And Company Polysaccharide coatings for paper
ES2937288T3 (es) 2015-10-26 2023-03-27 Nutrition & Biosciences Usa 4 Inc Composición de alfa-(1,3->glucano) insoluble en agua
WO2017083226A1 (en) 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
JP7045313B2 (ja) * 2015-11-13 2022-03-31 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
EP3374401B1 (en) 2015-11-13 2022-04-06 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
US10895028B2 (en) 2015-12-14 2021-01-19 Dupont Industrial Biosciences Usa, Llc Nonwoven glucan webs
US10266861B2 (en) * 2015-12-14 2019-04-23 E. I. Du Pont De Nemours And Company Production and composition of fructose syrup
BR112019012291A2 (pt) * 2016-12-15 2019-11-19 Danisco Us Inc. método para aumentar a produção de etanol a partir de fibra de milho em um processo de hidrólise de amido
JP2018139554A (ja) * 2017-02-28 2018-09-13 日本食品化工株式会社 水溶性食物繊維含有組成物及びその製造方法
JP7208924B2 (ja) 2017-05-23 2023-01-19 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド α－１，３－グルカンの酵素的製造
EP3415622A1 (en) * 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
EP3668970A1 (en) * 2017-09-13 2020-06-24 DuPont Industrial Biosciences USA, LLC Engineered glucosyltransferases
ES2920357T3 (es) * 2017-11-10 2022-08-03 Nutrition & Biosciences Usa 4 Inc Polisacárido morfológico único
EP3740583A1 (en) * 2018-02-23 2020-11-25 Danisco US Inc. Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes
CN115197884A (zh) * 2022-08-24 2022-10-18 吉林大学 一种降解废弃淀粉的微生物菌剂及其应用方法
CN120118963A (zh) * 2023-12-08 2025-06-10 宁夏伊品生物科技股份有限公司 aglA基因在制备L-氨基酸中的应用

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Publication number Publication date
EP3110958A1 (en) 2017-01-04
CN106460023A (zh) 2017-02-22
EP3110959A1 (en) 2017-01-04
AU2015223027B2 (en) 2018-09-20
WO2015130881A1 (en) 2015-09-03
CN106460023B (zh) 2026-02-24
JP7011393B2 (ja) 2022-02-10
CA2938144A1 (en) 2015-09-03
JP6929499B2 (ja) 2021-09-01
CN106068327A (zh) 2016-11-02
JP2017518026A (ja) 2017-07-06
AU2015223025A1 (en) 2016-08-04
AU2015223027A1 (en) 2016-08-04
BR112016019823A2 (pt) 2017-10-17
JP2017518025A (ja) 2017-07-06
CA2940778A1 (en) 2015-09-03
WO2015130883A1 (en) 2015-09-03
BR112016019823B1 (pt) 2023-05-09
WO2015130883A9 (en) 2016-08-18
BR112016019820A2 (pt) 2017-10-17

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