AU2013326968B2 - Multiprotein expression cassettes - Google Patents

Multiprotein expression cassettes Download PDF

Info

Publication number
AU2013326968B2
AU2013326968B2 AU2013326968A AU2013326968A AU2013326968B2 AU 2013326968 B2 AU2013326968 B2 AU 2013326968B2 AU 2013326968 A AU2013326968 A AU 2013326968A AU 2013326968 A AU2013326968 A AU 2013326968A AU 2013326968 B2 AU2013326968 B2 AU 2013326968B2
Authority
AU
Australia
Prior art keywords
gly
leu
val
ser
thr
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2013326968A
Other languages
English (en)
Other versions
AU2013326968A1 (en
Inventor
Jason Donald
R. Michael Raab
Binzhang Shen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Agrivida Inc
Original Assignee
Agrivida Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agrivida Inc filed Critical Agrivida Inc
Publication of AU2013326968A1 publication Critical patent/AU2013326968A1/en
Application granted granted Critical
Publication of AU2013326968B2 publication Critical patent/AU2013326968B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
    • C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
    • C11D3/16—Organic compounds
    • C11D3/38—Products with no well-defined composition, e.g. natural products
    • C11D3/386—Preparations containing enzymes, e.g. protease or amylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/503—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/90—Fusion polypeptide containing a motif for post-translational modification
    • C07K2319/92—Fusion polypeptide containing a motif for post-translational modification containing an intein ("protein splicing")domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/62—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21062—Subtilisin (3.4.21.62)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Polymers & Plastics (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AU2013326968A 2012-10-03 2013-10-03 Multiprotein expression cassettes Ceased AU2013326968B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261744863P 2012-10-03 2012-10-03
US61/744,863 2012-10-03
US201361783424P 2013-03-14 2013-03-14
US61/783,424 2013-03-14
PCT/US2013/063298 WO2014055778A2 (en) 2012-10-03 2013-10-03 Multiprotein expression cassettes

Publications (2)

Publication Number Publication Date
AU2013326968A1 AU2013326968A1 (en) 2015-04-09
AU2013326968B2 true AU2013326968B2 (en) 2019-08-08

Family

ID=50435440

Family Applications (3)

Application Number Title Priority Date Filing Date
AU2013326968A Ceased AU2013326968B2 (en) 2012-10-03 2013-10-03 Multiprotein expression cassettes
AU2013326972A Ceased AU2013326972B2 (en) 2012-10-03 2013-10-03 Intein-modified proteases, their production and industrial applications
AU2019208278A Ceased AU2019208278B2 (en) 2012-10-03 2019-07-29 Intein-modified proteases, their production and industrial applications

Family Applications After (2)

Application Number Title Priority Date Filing Date
AU2013326972A Ceased AU2013326972B2 (en) 2012-10-03 2013-10-03 Intein-modified proteases, their production and industrial applications
AU2019208278A Ceased AU2019208278B2 (en) 2012-10-03 2019-07-29 Intein-modified proteases, their production and industrial applications

Country Status (9)

Country Link
US (3) US10047352B2 (https=)
EP (1) EP2904101B1 (https=)
CN (1) CN104822830B (https=)
AU (3) AU2013326968B2 (https=)
BR (1) BR112015007466B1 (https=)
CA (1) CA2885931C (https=)
DK (1) DK2904101T3 (https=)
MX (1) MX368688B (https=)
WO (2) WO2014055778A2 (https=)

Families Citing this family (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
JP6591998B2 (ja) * 2014-03-28 2019-10-16 ノボザイムス アクティーゼルスカブ 低ｐＨでのタンパク質結晶の再可溶化
CA2950273C (en) * 2014-05-30 2022-06-21 Novozymes A/S Variants of gh family 11 xylanase and polynucleotides encoding same
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2016069774A1 (en) * 2014-10-28 2016-05-06 Agrivida, Inc. Methods and compositions for stabilizing trans-splicing intein modified proteases
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したＣａｓ９蛋白質
RU2619217C1 (ru) * 2015-12-04 2017-05-12 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов" (ФГБУ "ГосНИИгенетика") Температурочувствительный мутантный интеин для нерастворимой экспрессии предшественника целевого белка
CN105420220B (zh) * 2016-01-26 2018-10-09 华东理工大学 一种天冬氨酸类蛋白酶及其编码基因与应用
CN105925596A (zh) * 2016-02-23 2016-09-07 上海交通大学 基于内含肽的药用重组蛋白的合成方法
EP3475404A1 (en) 2016-06-23 2019-05-01 Novozymes A/S Use of enzymes, composition and method for removing soil
KR102547316B1 (ko) 2016-08-03 2023-06-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3526320A1 (en) 2016-10-14 2019-08-21 President and Fellows of Harvard College Aav delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
GB201702938D0 (en) 2017-02-23 2017-04-12 Univ Southampton Methods of generating and screening compartmentalised peptides libraries
JP2020510038A (ja) 2017-03-09 2020-04-02 プレジデント アンド フェローズ オブ ハーバード カレッジ がんワクチン
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
WO2019161304A1 (en) 2018-02-15 2019-08-22 Duke University Gene editing using homology-independent universal genome engineering technology
EP3546558A1 (en) * 2018-03-28 2019-10-02 The Procter & Gamble Company Laundry detergent composition
EP3797160A1 (en) 2018-05-23 2021-03-31 The Broad Institute Inc. Base editors and uses thereof
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
CN109456956B (zh) * 2018-11-06 2022-03-18 济南百斯杰生物工程有限公司 啤酒加工过程大麦副产物酶解处理用酶组合物及处理方法
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
JP7657726B2 (ja) 2019-03-19 2025-04-07 ザ ブロード インスティテュート，インコーポレーテッド 編集ヌクレオチド配列を編集するための方法および組成物
US20220204975A1 (en) 2019-04-12 2022-06-30 President And Fellows Of Harvard College System for genome editing
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
CN110172451A (zh) * 2019-05-05 2019-08-27 昆明理工大学 一种高通量分离噬菌体的方法
WO2020252455A1 (en) 2019-06-13 2020-12-17 The General Hospital Corporation Engineered human-endogenous virus-like particles and methods of use thereof for delivery to cells
EP4010474A1 (en) 2019-08-08 2022-06-15 The Broad Institute, Inc. Base editors with diversified targeting scope
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
CN110846299B (zh) * 2019-11-22 2021-09-24 江南大学 一种前导肽突变体及其在角蛋白酶生产中的应用
WO2021158999A1 (en) 2020-02-05 2021-08-12 The Broad Institute, Inc. Gene editing methods for treating spinal muscular atrophy
US20230127008A1 (en) 2020-03-11 2023-04-27 The Broad Institute, Inc. Stat3-targeted base editor therapeutics for the treatment of melanoma and other cancers
WO2021222318A1 (en) 2020-04-28 2021-11-04 The Broad Institute, Inc. Targeted base editing of the ush2a gene
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
EP4185600A4 (en) 2020-07-24 2024-12-04 The General Hospital Corporation Enhanced virus-like particles and methods of use thereof for delivery to cells
EP4217490A2 (en) 2020-09-24 2023-08-02 The Broad Institute Inc. Prime editing guide rnas, compositions thereof, and methods of using the same
EP4274894A2 (en) 2021-01-11 2023-11-15 The Broad Institute, Inc. Prime editor variants, constructs, and methods for enhancing prime editing efficiency and precision
CN113403209B (zh) * 2021-07-30 2022-08-26 西南大学 天冬氨酸蛋白酶基因在改良球孢白僵菌菌株中的应用
WO2023015309A2 (en) 2021-08-06 2023-02-09 The Broad Institute, Inc. Improved prime editors and methods of use
WO2023076898A1 (en) 2021-10-25 2023-05-04 The Broad Institute, Inc. Methods and compositions for editing a genome with prime editing and a recombinase
WO2023102538A1 (en) 2021-12-03 2023-06-08 The Broad Institute, Inc. Self-assembling virus-like particles for delivery of prime editors and methods of making and using same
WO2023205687A1 (en) 2022-04-20 2023-10-26 The Broad Institute, Inc. Improved prime editing methods and compositions
CN119630280A (zh) 2022-05-14 2025-03-14 诺维信公司 用于预防、处理、抑制和/或消除植物病原性侵染和感染的组合物和方法
US20250376674A1 (en) 2022-06-23 2025-12-11 Prime Medicine, Inc. Split prime editors
CN115925830B (zh) * 2022-08-15 2023-11-07 广州市乾相生物科技有限公司 一种内含肽变体及其在生物法制备类蛇毒肽前体中的应用
WO2024077267A1 (en) 2022-10-07 2024-04-11 The Broad Institute, Inc. Prime editing methods and compositions for treating triplet repeat disorders
EP4619515A1 (en) 2022-11-17 2025-09-24 The Broad Institute, Inc. Prime editor delivery by aav
CN116731126B (zh) * 2023-01-30 2024-02-23 态创生物科技(广州)有限公司 内含肽ChiATP、内含肽ChiATP-二肽-2融合蛋白及二肽-2的表达方法
WO2024163862A2 (en) 2023-02-03 2024-08-08 The Broad Institute, Inc. Gene editing methods, systems, and compositions for treating spinal muscular atrophy
WO2025165968A1 (en) * 2024-01-31 2025-08-07 Danisco Us Inc. Conditional regulation of protein function in filamentous fungal cells

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AT366877B (de) 1980-07-28 1982-05-10 Voest Alpine Ag Gewaechshaus
US5543302A (en) 1988-05-27 1996-08-06 Solvay Enzymes, Inc. Proteases of altered stability to autolytic degradation
DE4023458A1 (de) * 1989-08-31 1991-03-07 Kali Chemie Ag Neue hochalkalische proteasen
US6369011B1 (en) 1997-06-04 2002-04-09 The Procter & Gamble Company Protease enzymes for tough cleaning and/or spot and film reduction and compositions incorporating same
JP4749548B2 (ja) * 1998-09-30 2011-08-17 ニユー・イングランド・バイオレイブズ・インコーポレイテツド インテイン媒介ペプチド連結
ES2260956T3 (es) * 1998-12-18 2006-11-01 The Penn State Research Foundation Ciclacion de peptidos mediada por inteina.
WO2000047751A1 (en) * 1999-02-12 2000-08-17 New England Biolabs, Inc. Intein-mediated protein ligation of expressed proteins
CA2362737A1 (en) * 1999-03-05 2000-09-08 Maxygen, Inc. Recombination of insertion modified nucleic acids
WO2001012820A1 (en) * 1999-08-17 2001-02-22 Health Research Institute Genetic system and self-cleaving inteins derived therefrom, bioseparations and protein purification employing same, and methods for determining critical, generalizable amino acid residues for varying intein activity
US20040091966A1 (en) 1999-08-30 2004-05-13 Martin Zeidler Polypeptide regulation by conditional inteins
US6855548B2 (en) 2000-02-08 2005-02-15 F. Hoffman-La Roche Ag Use of acid-stable proteases in animal feed
ES2336641T3 (es) 2000-02-11 2010-04-15 Metabolix, Inc. Constructos de expresion multigenicos que contienen inteinas modificadas.
ATE490968T1 (de) * 2001-05-04 2010-12-15 Cornell Res Foundation Inc Schnell spaltbares sumofusionsprotein- expressionssystem für schwer zu exprimierende proteine
AU2003235745C1 (en) 2002-01-08 2009-09-24 R. Michael Raab Transgenic plants expressing CIVPS or intein modified proteins and related method
US20030167533A1 (en) 2002-02-04 2003-09-04 Yadav Narendra S. Intein-mediated protein splicing
JP4567456B2 (ja) 2002-08-09 2010-10-20 ノース・キャロライナ・ステイト・ユニヴァーシティ 肉用家禽類の成長を改善するための方法および組成物
ES2291883T3 (es) 2003-02-07 2008-03-01 Novozymes A/S Proteasas.
WO2005098043A2 (en) * 2004-03-30 2005-10-20 The President And Fellows Of Harvard College Ligand-dependent protein splicing
EP1751288A2 (en) 2004-05-19 2007-02-14 Agrivida, Inc. Transgenic plants expressing intein modified proteins and associated processes for bio-pharmaceutical production
EP2548955A1 (en) 2006-02-14 2013-01-23 Verenium Corporation Xylanases, nucleic acids encoding them and methods for making and using them
US8420387B2 (en) 2009-11-06 2013-04-16 Agrivida, Inc. Intein-modified enzymes, their production and industrial applications
ES2579054T3 (es) 2010-08-27 2016-08-04 Agrivida, Inc. Desarrollo de una característica de procesamiento celulósico utilizando una xilanasa modificada por una inteína termorregulada
CA2809916A1 (en) 2010-10-01 2012-04-05 Novozymes A/S Polypeptides having endopeptidase activity and polynucleotides encoding same
EP2761006B1 (en) * 2011-09-28 2016-12-14 Zera Intein Protein Solutions, S.L. Split inteins and uses thereof
EP2915817B1 (en) 2012-10-31 2018-06-20 Hybio Pharmaceutical Co., Ltd. Method for preparing exenatide

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HALL, M., ET AL., "Creation of an artificial bifunctional intein by grafting a homing endonuclease into a mini-intein.", Journal of Molecular Biology, 2002, 323.2 : pages 173-179. *
LIANG, R., ET AL., "Construction of a bacterial assay for estrogen detection based on an estrogen-sensitive intein.", Applied and Environmental Microbiology, 2011, 77.7 : pages 2488-2495. *
RAMSDEN, R., ET AL., "An intein with genetically selectable markers provides a new approach to internally label proteins with GFP.", BMC Biotechnology, 2011, 11.1 : article 71. *
SENEJANI, A., ET AL., "Structural stability and endonuclease activity of a PI-SceI GFP-fusion protein.", International Journal of Biological Sciences, 2007, 3.4 : article 205. *
WU, W., ET AL., "Intein#mediated purification of cytotoxic endonuclease l# Tev I by insertional inactivation and pH#controllable splicing.", Nucleic Acids Research, 2002, 30.22 : pages 4864-4871. *

Also Published As

Publication number Publication date
EP2904101B1 (en) 2019-06-12
BR112015007466B1 (pt) 2022-10-11
US20180305675A1 (en) 2018-10-25
WO2014055778A2 (en) 2014-04-10
CN104822830A (zh) 2015-08-05
CA2885931A1 (en) 2014-04-10
WO2014055778A3 (en) 2014-05-01
EP2904101A1 (en) 2015-08-12
HK1211315A1 (en) 2016-05-20
AU2013326972A1 (en) 2015-04-09
DK2904101T3 (da) 2019-08-12
AU2019208278A1 (en) 2019-09-05
AU2019208278B2 (en) 2022-04-21
CN104822830B (zh) 2021-07-09
WO2014055782A1 (en) 2014-04-10
CA2885931C (en) 2023-01-10
MX2015004088A (es) 2015-11-06
MX368688B (es) 2019-10-11
AU2013326968A1 (en) 2015-04-09
US10047352B2 (en) 2018-08-14
US9963707B2 (en) 2018-05-08
US20150267206A1 (en) 2015-09-24
US20150232827A1 (en) 2015-08-20
US10851362B2 (en) 2020-12-01
AU2013326972B2 (en) 2019-08-08
BR112015007466A2 (https=) 2017-08-29
EP2904101A4 (en) 2016-07-27

Similar Documents

Publication Publication Date Title
AU2013326968B2 (en) Multiprotein expression cassettes
CN101939434B (zh) 用于在大豆中提高种子贮藏油脂的生成和改变脂肪酸谱的来自解脂耶氏酵母的dgat基因
CN108138121B (zh) 用微生物高水平生产长链二羧酸
CA2834053C (en) Yeast strains engineered to produce ethanol from glycerol
AU2017220789B2 (en) Compositions and Methods for Modifying Genomes
AU2014308899B2 (en) Methods for producing genetic modifications in a plant genome without incorporating a selectable transgene marker, and compositions thereof
DK2664670T3 (da) Perhydrolase
CN110684806A (zh) Fad2性能基因座及相应的能够诱导靶向断裂的靶位点特异性结合蛋白
KR20210149060A (ko) Tn7-유사 트랜스포존을 사용한 rna-유도된 dna 통합
AU2010258955B2 (en) Expression cassettes derived from maize
BRPI0620552A2 (pt) polinucleotìdeo isolado, polipeptìdeo delta-9 elongase, construção recombinante, célula vegetal, método para transformar uma célula, método para produção de uma planta transgênica, sementes transgênicas, método para fabricar ácidos graxos poliinsaturados de cadeia longa, óleos, método para produzir pelo menos um ácido graxo poliinsaturado, plantas de semente oleaginosa, sementes, alimentos, fragmento de ácido nucléico isolado e progênies de plantas
KR20130027063A (ko) Ｆｅ-ｓ 클러스터 요구성 단백질의 활성 향상
KR20130117753A (ko) 포스포케톨라아제를 포함하는 재조합 숙주 세포
CN101646766B (zh) △17去饱和酶及其用于制备多不饱和脂肪酸的用途
KR20140092759A (ko) 숙주 세포 및 아이소부탄올의 제조 방법
KR20220012327A (ko) 피토칸나비노이드 및 피토칸나비노이드 전구체의 생산을 위한 방법 및 세포
AU2024205703A1 (en) Wheat stem rust resistance genes and methods of use
KR20140050602A (ko) 콤팩트 tale-뉴클레아제의 발생 방법 및 이의 용도
KR20140113997A (ko) 부탄올 생성을 위한 유전자 스위치
KR20130032897A (ko) 알코올 발효 시의 알코올 에스테르의 생성 및 원위치에서의 생성물 제거
KR20140099224A (ko) 케토-아이소발레레이트 데카르복실라제 효소 및 이의 이용 방법
KR20180084135A (ko) 감소된 clr2 활성을 갖는 사상 진균에서 단백질을 생산하는 방법
PT1984512T (pt) Sistema de expressão génica utilizando excisão-união em insetos
KR20180081817A (ko) 감소된 clr1 활성을 갖는 사상 진균에서 단백질을 생산하는 방법
KR20230156365A (ko) 메타게놈 유래의 새로운 CRISPR-Cas 뉴클레아제

Legal Events

Date Code Title Description
DA3 Amendments made section 104

Free format text: THE NATURE OF THE AMENDMENT IS: AMEND THE NAME OF THE INVENTOR TO READ SHEN, BINZHANG; DONALD, JASON AND RAAB, R. MICHAEL

FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired