AU2012340344B2 - Microfluidic apparatus, method, and applications - Google Patents

Microfluidic apparatus, method, and applications Download PDF

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Publication number
AU2012340344B2
AU2012340344B2 AU2012340344A AU2012340344A AU2012340344B2 AU 2012340344 B2 AU2012340344 B2 AU 2012340344B2 AU 2012340344 A AU2012340344 A AU 2012340344A AU 2012340344 A AU2012340344 A AU 2012340344A AU 2012340344 B2 AU2012340344 B2 AU 2012340344B2
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AU
Australia
Prior art keywords
liquid
tube
nucleic acid
tissue sample
aqueous solution
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AU2012340344A
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English (en)
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AU2012340344A1 (en
Inventor
Peng Zhou
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Rheonix Inc
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Rheonix Inc
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • C07H1/06Separation; Purification
    • C07H1/08Separation; Purification from natural products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06Fluid handling related problems
    • B01L2200/0631Purification arrangements, e.g. solid phase extraction [SPE]

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Hematology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2012340344A 2011-11-17 2012-11-16 Microfluidic apparatus, method, and applications Active AU2012340344B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161561007P 2011-11-17 2011-11-17
US61/561,007 2011-11-17
PCT/US2012/065452 WO2013074885A1 (en) 2011-11-17 2012-11-16 Microfluidic apparatus, method, and applications

Publications (2)

Publication Number Publication Date
AU2012340344A1 AU2012340344A1 (en) 2014-05-01
AU2012340344B2 true AU2012340344B2 (en) 2015-10-29

Family

ID=48430176

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2012340344A Active AU2012340344B2 (en) 2011-11-17 2012-11-16 Microfluidic apparatus, method, and applications

Country Status (7)

Country Link
US (4) US8852919B2 (enExample)
EP (1) EP2780453B1 (enExample)
JP (3) JP2014533952A (enExample)
CN (1) CN104053774B (enExample)
AU (1) AU2012340344B2 (enExample)
IN (1) IN2014CN04296A (enExample)
WO (1) WO2013074885A1 (enExample)

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EP2468862A1 (en) * 2010-12-23 2012-06-27 Qiagen GmbH Method and kit for processing wax-embedded biological samples
AU2013323586B2 (en) * 2012-09-28 2019-07-11 Cepheid Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
CN204727866U (zh) 2014-03-31 2015-10-28 博奥生物集团有限公司 一种集核酸扩增和微阵列检测于一体的微流控装置
WO2016168429A1 (en) * 2015-04-14 2016-10-20 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University Method and apparatus for extracting and collecting single cells from formalin-fixed paraffin embedded tissues
DE102016208936A1 (de) * 2016-05-24 2017-11-30 Robert Bosch Gmbh Mikrofluidische Vorrichtung und Verfahren zur Probenaufbereitung von Paraffin-eigebettetem Gewebe
WO2018119295A1 (en) * 2016-12-22 2018-06-28 Ventana Medical Systems, Inc. Fully automated nucleic acid extraction method for tissue samples
DE102018200520A1 (de) * 2018-01-15 2019-07-18 Robert Bosch Gmbh Verfahren zum Bereitstellen einer Lösung der Substanz in einer mikrofluidischen Vorrichtung
US11513041B2 (en) 2018-10-19 2022-11-29 Polyvalor, Limited Partnership Medium-embedded samples
WO2020185585A1 (en) * 2019-03-08 2020-09-17 Rarecyte, Inc. Device, system, and method for selecting a target analyte
US11332777B1 (en) 2021-10-28 2022-05-17 URIT Medical Electronic Co., Ltd. Methods and reagent kits for bisulfite conversion of DNA
DE102022111890B3 (de) 2022-05-12 2023-03-16 Dermagnostix GmbH Verfahren zur Deparaffinierung von Formalin-fixiertem Paraffin-eingebettetem Gewebe

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110003369A1 (en) * 2009-07-01 2011-01-06 Kirsch Christoph Method for the deparaffinisation of biological specimens
WO2012085261A1 (en) * 2010-12-23 2012-06-28 Qiagen Gmbh Method and kit for processing wax-embedded biological samples

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US5552087A (en) * 1993-11-15 1996-09-03 Ventana Medical Systems, Inc. High temperature evaporation inhibitor liquid
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US6855559B1 (en) * 1998-09-03 2005-02-15 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US7410753B2 (en) 1998-09-03 2008-08-12 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6544798B1 (en) 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6469159B1 (en) 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
US7364846B2 (en) * 2002-10-11 2008-04-29 Molecular Devices Corporation Gene expression profiling from FFPE samples
US20050142570A1 (en) 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent
AU2006249956A1 (en) * 2005-05-24 2006-11-30 Angros, Lee H In situ heat induced antigen recovery and staining apparatus and method
US7544471B2 (en) * 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
WO2009049268A1 (en) * 2007-10-12 2009-04-16 Rheonix, Inc. Integrated microfluidic device and methods
DE102008029356A1 (de) 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
WO2010003463A1 (en) * 2008-07-11 2010-01-14 Metabolic Explorer Method for polymerising glycolic acid with microorganisms
DE102008061714A1 (de) * 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
WO2011008217A1 (en) 2009-07-17 2011-01-20 Canon U.S. Life Sciences, Inc. Methods and systems for dna isolation on a microfluidic device
WO2011104032A1 (en) * 2010-02-26 2011-09-01 Qiagen Gmbh Method for isolating rna from a rna and dna containing sample
JP2011213804A (ja) * 2010-03-31 2011-10-27 Sumitomo Chemical Co Ltd 予備重合方法、予備重合済付加重合触媒成分およびそれを用いる付加重合体の製造方法
WO2012024103A2 (en) 2010-08-17 2012-02-23 Rheonix, Inc. Thermocycler seal composition, method, and application
AU2011315926B2 (en) 2010-10-14 2015-12-10 Rheonix, Inc. Quantitative multiplexed identification of nucleic acid targets

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110003369A1 (en) * 2009-07-01 2011-01-06 Kirsch Christoph Method for the deparaffinisation of biological specimens
WO2012085261A1 (en) * 2010-12-23 2012-06-28 Qiagen Gmbh Method and kit for processing wax-embedded biological samples

Also Published As

Publication number Publication date
AU2012340344A1 (en) 2014-05-01
JP2018148904A (ja) 2018-09-27
US10081828B2 (en) 2018-09-25
US20140377854A1 (en) 2014-12-25
JP6655652B2 (ja) 2020-02-26
CN104053774B (zh) 2016-09-07
EP2780453A1 (en) 2014-09-24
US20210054440A1 (en) 2021-02-25
US20130164826A1 (en) 2013-06-27
IN2014CN04296A (enExample) 2015-09-04
CN104053774A (zh) 2014-09-17
US8852919B2 (en) 2014-10-07
EP2780453B1 (en) 2017-03-29
EP2780453A4 (en) 2015-06-03
JP2014533952A (ja) 2014-12-18
US10865436B2 (en) 2020-12-15
US20150056650A1 (en) 2015-02-26
US11795493B2 (en) 2023-10-24
WO2013074885A1 (en) 2013-05-23
JP2016187355A (ja) 2016-11-04

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