CN104053774B - 微流体装置、方法和应用 - Google Patents

微流体装置、方法和应用 Download PDF

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Publication number
CN104053774B
CN104053774B CN201280056605.1A CN201280056605A CN104053774B CN 104053774 B CN104053774 B CN 104053774B CN 201280056605 A CN201280056605 A CN 201280056605A CN 104053774 B CN104053774 B CN 104053774B
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liquid
tube
nucleic acid
tissue sample
aqueous solution
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CN201280056605.1A
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Chinese (zh)
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CN104053774A (zh
Inventor
周朋
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Sscd Enterprise Co ltd
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Rheonix Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0631—Purification arrangements, e.g. solid phase extraction [SPE]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201280056605.1A 2011-11-17 2012-11-16 微流体装置、方法和应用 Active CN104053774B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201161561007P 2011-11-17 2011-11-17
US61/561,007 2011-11-17
PCT/US2012/065452 WO2013074885A1 (en) 2011-11-17 2012-11-16 Microfluidic apparatus, method, and applications

Publications (2)

Publication Number Publication Date
CN104053774A CN104053774A (zh) 2014-09-17
CN104053774B true CN104053774B (zh) 2016-09-07

Family

ID=48430176

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201280056605.1A Active CN104053774B (zh) 2011-11-17 2012-11-16 微流体装置、方法和应用

Country Status (7)

Country Link
US (4) US8852919B2 (enExample)
EP (1) EP2780453B1 (enExample)
JP (3) JP2014533952A (enExample)
CN (1) CN104053774B (enExample)
AU (1) AU2012340344B2 (enExample)
IN (1) IN2014CN04296A (enExample)
WO (1) WO2013074885A1 (enExample)

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EP2468862A1 (en) * 2010-12-23 2012-06-27 Qiagen GmbH Method and kit for processing wax-embedded biological samples
CA2886389A1 (en) * 2012-09-28 2014-04-03 Cepheid Methods for dna and rna extraction from fixed paraffin-embedded tissue samples
CN104946510B (zh) 2014-03-31 2017-06-06 博奥生物集团有限公司 集核酸扩增和微阵列检测于一体的微流控装置
WO2016168429A1 (en) * 2015-04-14 2016-10-20 Arizona Board Of Regents, A Body Corporate Of The State Of Arizona, Acting For And On Behalf Of Arizona State University Method and apparatus for extracting and collecting single cells from formalin-fixed paraffin embedded tissues
DE102016208936A1 (de) * 2016-05-24 2017-11-30 Robert Bosch Gmbh Mikrofluidische Vorrichtung und Verfahren zur Probenaufbereitung von Paraffin-eigebettetem Gewebe
CN110050070B (zh) * 2016-12-22 2023-12-22 文塔纳医疗系统公司 用于组织样品的全自动化核酸提取方法
DE102018200520A1 (de) 2018-01-15 2019-07-18 Robert Bosch Gmbh Verfahren zum Bereitstellen einer Lösung der Substanz in einer mikrofluidischen Vorrichtung
US11513041B2 (en) 2018-10-19 2022-11-29 Polyvalor, Limited Partnership Medium-embedded samples
JP2022524120A (ja) * 2019-03-08 2022-04-27 レアサイト インコーポレイテッド 標的分析物を選択する器具、システムおよび方法
US11332777B1 (en) * 2021-10-28 2022-05-17 URIT Medical Electronic Co., Ltd. Methods and reagent kits for bisulfite conversion of DNA
DE102022111890B3 (de) 2022-05-12 2023-03-16 Dermagnostix GmbH Verfahren zur Deparaffinierung von Formalin-fixiertem Paraffin-eingebettetem Gewebe

Citations (5)

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US20040072305A1 (en) * 2002-10-11 2004-04-15 Erlander Mark G. Gene expression profiling from FFPE samples
US20050142570A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent
US20110003369A1 (en) * 2009-07-01 2011-01-06 Kirsch Christoph Method for the deparaffinisation of biological specimens
WO2011008217A1 (en) * 2009-07-17 2011-01-20 Canon U.S. Life Sciences, Inc. Methods and systems for dna isolation on a microfluidic device
US20110245438A1 (en) * 2010-03-31 2011-10-06 Sumitomo Chemical Company, Limited Production process of pre-polymerized polymerization catalyst component and addition polymer

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US5552087A (en) * 1993-11-15 1996-09-03 Ventana Medical Systems, Inc. High temperature evaporation inhibitor liquid
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5863502A (en) * 1996-01-24 1999-01-26 Sarnoff Corporation Parallel reaction cassette and associated devices
US6855559B1 (en) * 1998-09-03 2005-02-15 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US7410753B2 (en) 1998-09-03 2008-08-12 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6544798B1 (en) 1999-02-26 2003-04-08 Ventana Medical Systems, Inc. Removal of embedding media from biological samples and cell conditioning on automated staining instruments
US6469159B1 (en) 1999-04-26 2002-10-22 Robert T. Belly Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
EP1888739B1 (en) * 2005-05-24 2021-08-11 Lee H. Angros Automated apparatus and method for treating biological specimens on slides
US7544471B2 (en) * 2005-07-30 2009-06-09 Agilent Technologies, Inc. Preparing RNA from a wax-embedded tissue specimen
JP5523327B2 (ja) * 2007-10-12 2014-06-18 レオニックス，インコーポレイテッド 統合型マイクロ流体デバイスおよび方法
DE102008029356A1 (de) 2008-06-20 2009-12-24 Siemens Healthcare Diagnostics Gmbh Verfahren zur Aufreinigung von Nukleinsäuren, insbesondere aus fixiertem Gewebe
WO2010003463A1 (en) * 2008-07-11 2010-01-14 Metabolic Explorer Method for polymerising glycolic acid with microorganisms
DE102008061714A1 (de) 2008-12-12 2010-06-17 Siemens Healthcare Diagnostics Inc., Deerfield Verfahren zur Aufreinigung von Nukleinsäuren, inbesondere aus fixiertem Gewebe
US10273470B2 (en) 2010-02-26 2019-04-30 Qiagen Gmbh Method for isolating RNA from a RNA and DNA containing sample
WO2012024103A2 (en) 2010-08-17 2012-02-23 Rheonix, Inc. Thermocycler seal composition, method, and application
WO2012051504A2 (en) 2010-10-14 2012-04-19 Rheonix, Inc. Quantitative multiplexed identification of nucleic acid targets
EP2468862A1 (en) * 2010-12-23 2012-06-27 Qiagen GmbH Method and kit for processing wax-embedded biological samples

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040072305A1 (en) * 2002-10-11 2004-04-15 Erlander Mark G. Gene expression profiling from FFPE samples
US20050142570A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent
US20110003369A1 (en) * 2009-07-01 2011-01-06 Kirsch Christoph Method for the deparaffinisation of biological specimens
WO2011008217A1 (en) * 2009-07-17 2011-01-20 Canon U.S. Life Sciences, Inc. Methods and systems for dna isolation on a microfluidic device
US20110245438A1 (en) * 2010-03-31 2011-10-06 Sumitomo Chemical Company, Limited Production process of pre-polymerized polymerization catalyst component and addition polymer

Also Published As

Publication number Publication date
US10865436B2 (en) 2020-12-15
JP2014533952A (ja) 2014-12-18
JP2018148904A (ja) 2018-09-27
EP2780453A4 (en) 2015-06-03
US20150056650A1 (en) 2015-02-26
CN104053774A (zh) 2014-09-17
US8852919B2 (en) 2014-10-07
EP2780453A1 (en) 2014-09-24
IN2014CN04296A (enExample) 2015-09-04
US10081828B2 (en) 2018-09-25
US11795493B2 (en) 2023-10-24
JP6655652B2 (ja) 2020-02-26
WO2013074885A1 (en) 2013-05-23
US20210054440A1 (en) 2021-02-25
JP2016187355A (ja) 2016-11-04
AU2012340344A1 (en) 2014-05-01
AU2012340344B2 (en) 2015-10-29
US20140377854A1 (en) 2014-12-25
US20130164826A1 (en) 2013-06-27
EP2780453B1 (en) 2017-03-29

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