AU2012293402B2 - Methods and materials for recombinant production of saffron compounds - Google Patents

Methods and materials for recombinant production of saffron compounds Download PDF

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Publication number
AU2012293402B2
AU2012293402B2 AU2012293402A AU2012293402A AU2012293402B2 AU 2012293402 B2 AU2012293402 B2 AU 2012293402B2 AU 2012293402 A AU2012293402 A AU 2012293402A AU 2012293402 A AU2012293402 A AU 2012293402A AU 2012293402 B2 AU2012293402 B2 AU 2012293402B2
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host cell
crocetin
nucleic acid
seq
exogenous nucleic
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AU2012293402A1 (en
AU2012293402A2 (en
Inventor
Esben Halkjaer Hansen
Jorgen Hansen
Klavs Riishede Hansen
Kalyan K. Kumar
Sathish Kumar
Murali PANCHAPAGESA
Shriram RAGHAVAN
Shailendra SONKAR
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Danstar Ferment AG
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Evolva AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C12N1/185—Saccharomyces isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/88—Lyases (4.)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185—Escherichia
    • C12R2001/19—Escherichia coli
    • C—CHEMISTRY; METALLURGY
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/85—Saccharomyces
    • C12R2001/865—Saccharomyces cerevisiae

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  • Life Sciences & Earth Sciences (AREA)
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  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Cell Biology (AREA)
AU2012293402A 2011-08-08 2012-08-07 Methods and materials for recombinant production of saffron compounds Ceased AU2012293402B2 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201161521171P 2011-08-08 2011-08-08
US61/521,171 2011-08-08
US201161576460P 2011-12-16 2011-12-16
US61/576,460 2011-12-16
US201261595450P 2012-02-06 2012-02-06
US61/595,450 2012-02-06
PCT/IB2012/001513 WO2013021261A2 (en) 2011-08-08 2012-08-07 Methods and materials for recombinant production of saffron compounds

Publications (3)

Publication Number Publication Date
AU2012293402A1 AU2012293402A1 (en) 2014-02-06
AU2012293402A2 AU2012293402A2 (en) 2014-03-13
AU2012293402B2 true AU2012293402B2 (en) 2017-08-03

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AU2012293402A Ceased AU2012293402B2 (en) 2011-08-08 2012-08-07 Methods and materials for recombinant production of saffron compounds

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US (2) US20140248668A1 (enExample)
EP (1) EP2742131B1 (enExample)
JP (1) JP6126597B2 (enExample)
KR (2) KR101983115B1 (enExample)
CN (1) CN103764818B (enExample)
AU (1) AU2012293402B2 (enExample)
BR (1) BR112014002924A2 (enExample)
CA (1) CA2843549A1 (enExample)
HK (1) HK1199286A1 (enExample)
IN (1) IN2014CN01129A (enExample)
RU (1) RU2676730C2 (enExample)
WO (1) WO2013021261A2 (enExample)

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US10017804B2 (en) 2013-02-11 2018-07-10 Evolva Sa Efficient production of steviol glycosides in recombinant hosts
WO2015132411A2 (en) * 2014-03-07 2015-09-11 Evolva Sa Methods for recombinant production of saffron compounds
EP3134539A1 (en) * 2014-04-25 2017-03-01 Evolva SA Methods for recombinant production of saffron compounds
CA3109156C (en) * 2014-05-16 2023-08-15 Academia Sinica Recombinant polynucleotide sequence for producing astaxanthin and uses thereof
US9969989B2 (en) 2014-07-23 2018-05-15 Agenzia Nazionale Per Le Nuove Tecnologie, L'ener- Gia E Lo Sviluppo Economico Sostenibile (Enea) Carotenoid dioxygenase and methods for the biotechnological production in microorganisms and plants of compounds derived from saffron
MX2017001859A (es) 2014-08-11 2017-04-11 Evolva Sa Produccion de glicosidos de esteviol en hospederos recombinantes.
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BR112017016338A2 (en) 2015-01-30 2018-03-27 Evolva Sa production of steviol glycosides in recombinant hosts
CA3255837A1 (en) 2015-03-16 2025-10-30 Dsm Ip Assets B.V. Udp-glycosyltransferases
JP2018522569A (ja) 2015-08-07 2018-08-16 エヴォルヴァ エスアー．Ｅｖｏｌｖａ Ｓａ． 組換え宿主内でのステビオールグリコシドの産生
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MY198001A (en) 2016-04-13 2023-07-25 Evolva SA Production of steviol glycoside in recombinant hosts
WO2017198682A1 (en) 2016-05-16 2017-11-23 Evolva Sa Production of steviol glycosides in recombinant hosts
CN105779406B (zh) * 2016-05-25 2019-06-18 新疆农业科学院经济作物研究所 一种藏红花酸糖基转移酶及其编码基因与应用
CN105950493B (zh) * 2016-05-25 2019-12-27 天津大学 一种工程菌及其构建方法与在制备藏红花酸中的应用
CN105950580B (zh) * 2016-05-25 2019-03-08 新疆农业科学院经济作物研究所 一种制备蛋白质UGTCs4的方法
BR112019000895A2 (pt) 2016-07-19 2019-04-30 Conagen Inc. método para a produção microbiana de capsaicinoides naturais específicos
US11396669B2 (en) 2016-11-07 2022-07-26 Evolva Sa Production of steviol glycosides in recombinant hosts
CN108728477B (zh) * 2017-04-24 2022-02-22 华东理工大学 一种高效的转座突变系统及构建方法
IT201700089843A1 (it) * 2017-08-03 2019-02-03 Enea Agenzia Naz Per Le Nuove Tecnologie Lenergia E Lo Sviluppo Economico Sostenibile Geni e metodi per la produzione e compartimentazione biotecnologica di apocarotenoidi ad elevato valore aggiunto
CN110791510A (zh) * 2018-08-02 2020-02-14 中国医学科学院药用植物研究所 一种参与西红花酸合成的乙醛脱氢酶编码基因的筛选鉴定及应用
WO2020093285A1 (zh) * 2018-11-07 2020-05-14 中国医学科学院药用植物研究所 用于生物合成西红花苷的酶基因及其应用
CA3118924A1 (en) 2018-11-09 2020-05-14 Ginkgo Bioworks, Inc. Biosynthesis of mogrosides
CN109943493B (zh) * 2019-04-17 2021-05-18 天津大学 实现通用酶催化功能多样性的突变体菌株及其构建方法
KR102335035B1 (ko) * 2019-09-17 2021-12-06 아주대학교 산학협력단 효소반응을 이용한 크로신-4의 생산방법
EP4151732A1 (en) * 2021-09-15 2023-03-22 Universidad de Castilla-La Mancha Transgenic plants producing high levels of apocarotenoids compounds and uses thereof
CN117467633A (zh) * 2022-07-28 2024-01-30 上海中医药大学 Ugt71a3作为催化剂在制备三萜皂苷中的应用
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KR101983115B1 (ko) 2019-05-29
KR101815063B1 (ko) 2018-01-05
KR20140057319A (ko) 2014-05-12
CN103764818B (zh) 2017-05-31
US20140248668A1 (en) 2014-09-04
JP6126597B2 (ja) 2017-05-10
HK1199286A1 (en) 2015-06-26
EP2742131A2 (en) 2014-06-18
WO2013021261A2 (en) 2013-02-14
RU2014109010A (ru) 2015-09-20
IN2014CN01129A (enExample) 2015-04-10
WO2013021261A3 (en) 2013-05-02
EP2742131B1 (en) 2018-11-28
JP2014524246A (ja) 2014-09-22
CA2843549A1 (en) 2013-02-14
CN103764818A (zh) 2014-04-30
US20170306376A1 (en) 2017-10-26
AU2012293402A1 (en) 2014-02-06
RU2676730C2 (ru) 2019-01-10
AU2012293402A2 (en) 2014-03-13
KR20180004306A (ko) 2018-01-10
BR112014002924A2 (pt) 2018-10-09

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