RU2676730C2 - Способы и вещества для основанного на рекомбинации получения соединений шафрана - Google Patents
Способы и вещества для основанного на рекомбинации получения соединений шафрана Download PDFInfo
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- RU2676730C2 RU2676730C2 RU2014109010A RU2014109010A RU2676730C2 RU 2676730 C2 RU2676730 C2 RU 2676730C2 RU 2014109010 A RU2014109010 A RU 2014109010A RU 2014109010 A RU2014109010 A RU 2014109010A RU 2676730 C2 RU2676730 C2 RU 2676730C2
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- host cell
- crocetin
- nucleic acid
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Botany (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Cell Biology (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161521171P | 2011-08-08 | 2011-08-08 | |
| US61/521,171 | 2011-08-08 | ||
| US201161576460P | 2011-12-16 | 2011-12-16 | |
| US61/576,460 | 2011-12-16 | ||
| US201261595450P | 2012-02-06 | 2012-02-06 | |
| US61/595,450 | 2012-02-06 | ||
| PCT/IB2012/001513 WO2013021261A2 (en) | 2011-08-08 | 2012-08-07 | Methods and materials for recombinant production of saffron compounds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2014109010A RU2014109010A (ru) | 2015-09-20 |
| RU2676730C2 true RU2676730C2 (ru) | 2019-01-10 |
Family
ID=46970359
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2014109010A RU2676730C2 (ru) | 2011-08-08 | 2012-08-07 | Способы и вещества для основанного на рекомбинации получения соединений шафрана |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US20140248668A1 (enExample) |
| EP (1) | EP2742131B1 (enExample) |
| JP (1) | JP6126597B2 (enExample) |
| KR (2) | KR101983115B1 (enExample) |
| CN (1) | CN103764818B (enExample) |
| AU (1) | AU2012293402B2 (enExample) |
| BR (1) | BR112014002924A2 (enExample) |
| CA (1) | CA2843549A1 (enExample) |
| HK (1) | HK1199286A1 (enExample) |
| IN (1) | IN2014CN01129A (enExample) |
| RU (1) | RU2676730C2 (enExample) |
| WO (1) | WO2013021261A2 (enExample) |
Families Citing this family (32)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105671108A (zh) | 2010-06-02 | 2016-06-15 | 沃维公司 | 甜菊糖苷的重组生产 |
| CA2899276C (en) | 2013-02-06 | 2022-11-01 | Evolva Sa | Methods for improved production of rebaudioside d and rebaudioside m |
| US10017804B2 (en) | 2013-02-11 | 2018-07-10 | Evolva Sa | Efficient production of steviol glycosides in recombinant hosts |
| WO2015132411A2 (en) * | 2014-03-07 | 2015-09-11 | Evolva Sa | Methods for recombinant production of saffron compounds |
| EP3134539A1 (en) * | 2014-04-25 | 2017-03-01 | Evolva SA | Methods for recombinant production of saffron compounds |
| CA3109156C (en) * | 2014-05-16 | 2023-08-15 | Academia Sinica | Recombinant polynucleotide sequence for producing astaxanthin and uses thereof |
| US9969989B2 (en) | 2014-07-23 | 2018-05-15 | Agenzia Nazionale Per Le Nuove Tecnologie, L'ener- Gia E Lo Sviluppo Economico Sostenibile (Enea) | Carotenoid dioxygenase and methods for the biotechnological production in microorganisms and plants of compounds derived from saffron |
| MX2017001859A (es) | 2014-08-11 | 2017-04-11 | Evolva Sa | Produccion de glicosidos de esteviol en hospederos recombinantes. |
| JP2017528134A (ja) * | 2014-09-09 | 2017-09-28 | エヴォルヴァ エスアー.Evolva Sa. | 組み換え宿主におけるステビオールグリコシドの生産 |
| BR112017016338A2 (en) | 2015-01-30 | 2018-03-27 | Evolva Sa | production of steviol glycosides in recombinant hosts |
| CA3255837A1 (en) | 2015-03-16 | 2025-10-30 | Dsm Ip Assets B.V. | Udp-glycosyltransferases |
| JP2018522569A (ja) | 2015-08-07 | 2018-08-16 | エヴォルヴァ エスアー.Evolva Sa. | 組換え宿主内でのステビオールグリコシドの産生 |
| AU2017205501A1 (en) | 2016-01-07 | 2018-07-05 | Conagen Inc. | Methods of making capsinoids by biosynthetic processes |
| MY198001A (en) | 2016-04-13 | 2023-07-25 | Evolva SA | Production of steviol glycoside in recombinant hosts |
| WO2017198682A1 (en) | 2016-05-16 | 2017-11-23 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CN105779406B (zh) * | 2016-05-25 | 2019-06-18 | 新疆农业科学院经济作物研究所 | 一种藏红花酸糖基转移酶及其编码基因与应用 |
| CN105950493B (zh) * | 2016-05-25 | 2019-12-27 | 天津大学 | 一种工程菌及其构建方法与在制备藏红花酸中的应用 |
| CN105950580B (zh) * | 2016-05-25 | 2019-03-08 | 新疆农业科学院经济作物研究所 | 一种制备蛋白质UGTCs4的方法 |
| BR112019000895A2 (pt) | 2016-07-19 | 2019-04-30 | Conagen Inc. | método para a produção microbiana de capsaicinoides naturais específicos |
| US11396669B2 (en) | 2016-11-07 | 2022-07-26 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
| CN108728477B (zh) * | 2017-04-24 | 2022-02-22 | 华东理工大学 | 一种高效的转座突变系统及构建方法 |
| IT201700089843A1 (it) * | 2017-08-03 | 2019-02-03 | Enea Agenzia Naz Per Le Nuove Tecnologie Lenergia E Lo Sviluppo Economico Sostenibile | Geni e metodi per la produzione e compartimentazione biotecnologica di apocarotenoidi ad elevato valore aggiunto |
| CN110791510A (zh) * | 2018-08-02 | 2020-02-14 | 中国医学科学院药用植物研究所 | 一种参与西红花酸合成的乙醛脱氢酶编码基因的筛选鉴定及应用 |
| WO2020093285A1 (zh) * | 2018-11-07 | 2020-05-14 | 中国医学科学院药用植物研究所 | 用于生物合成西红花苷的酶基因及其应用 |
| CA3118924A1 (en) | 2018-11-09 | 2020-05-14 | Ginkgo Bioworks, Inc. | Biosynthesis of mogrosides |
| CN109943493B (zh) * | 2019-04-17 | 2021-05-18 | 天津大学 | 实现通用酶催化功能多样性的突变体菌株及其构建方法 |
| KR102335035B1 (ko) * | 2019-09-17 | 2021-12-06 | 아주대학교 산학협력단 | 효소반응을 이용한 크로신-4의 생산방법 |
| EP4151732A1 (en) * | 2021-09-15 | 2023-03-22 | Universidad de Castilla-La Mancha | Transgenic plants producing high levels of apocarotenoids compounds and uses thereof |
| CN117467633A (zh) * | 2022-07-28 | 2024-01-30 | 上海中医药大学 | Ugt71a3作为催化剂在制备三萜皂苷中的应用 |
| CN116083288B (zh) * | 2022-11-11 | 2025-02-18 | 泸州品创科技有限公司 | 产2,3-二氢-2,2,6-三甲基苯甲醛的肠膜明串珠菌及其应用 |
| KR20250075325A (ko) * | 2023-11-21 | 2025-05-28 | 아주대학교산학협력단 | 크로신 및 전구체 생산용 재조합 효모 |
| CN118546994B (zh) * | 2024-06-18 | 2025-01-28 | 华南农业大学 | 一种在种子胚乳生产藏红花素的转基因育种方法 |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2003108882A (ru) * | 2000-09-01 | 2004-12-10 | Е.И.Дюпон Де Немур Энд Компани | Продуцирование каротиноидов из одноуглеродного субстрата |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6946587B1 (en) | 1990-01-22 | 2005-09-20 | Dekalb Genetics Corporation | Method for preparing fertile transgenic corn plants |
| US5484956A (en) | 1990-01-22 | 1996-01-16 | Dekalb Genetics Corporation | Fertile transgenic Zea mays plant comprising heterologous DNA encoding Bacillus thuringiensis endotoxin |
| US5204253A (en) | 1990-05-29 | 1993-04-20 | E. I. Du Pont De Nemours And Company | Method and apparatus for introducing biological substances into living cells |
| US5306862A (en) | 1990-10-12 | 1994-04-26 | Amoco Corporation | Method and composition for increasing sterol accumulation in higher plants |
| US5460949A (en) | 1990-11-15 | 1995-10-24 | Amoco Corporation | Method and composition for increasing the accumulation of squalene and specific sterols in yeast |
| DK0747483T3 (da) * | 1995-06-09 | 2004-06-28 | Dsm Ip Assets Bv | Fermentativ carotenoid-produktion |
| JPH10117776A (ja) | 1996-10-22 | 1998-05-12 | Japan Tobacco Inc | インディカイネの形質転換方法 |
| ATE242812T1 (de) * | 1996-12-02 | 2003-06-15 | Hoffmann La Roche | Verbesserte fermentative herstellung von carotenoide |
| DE19916140A1 (de) * | 1999-04-09 | 2000-10-12 | Basf Ag | Carotinhydroxylase und Verfahren zur Herstellung von Xanthophyllderivaten |
| PT1190068E (pt) | 1999-04-15 | 2006-06-30 | Calgene Llc | Sequencias de acidos nucleicos para proteinas envolvidas na sintese de tocoferol |
| US20040078846A1 (en) | 2002-01-25 | 2004-04-22 | Desouza Mervyn L. | Carotenoid biosynthesis |
| US20050003474A1 (en) | 2001-01-26 | 2005-01-06 | Desouza Mervyn L. | Carotenoid biosynthesis |
| EP1532265A2 (de) * | 2002-08-20 | 2005-05-25 | Sungene GmbH & Co. KGaA | Verfahren zur herstellung von ketocarotinoiden in genetisch veränderten organismen |
| JP2006500055A (ja) * | 2002-09-27 | 2006-01-05 | ディーエスエム アイピー アセッツ ビー.ブイ. | ファフィア属によるゼアキサンチンの製造方法 |
| EP1765418B1 (en) | 2004-05-21 | 2011-12-14 | The Regents of the University of California | Method for enhancing production of isoprenoid compounds |
| CN101321859A (zh) * | 2005-12-06 | 2008-12-10 | 东曹株式会社 | 新微生物和使用该微生物生产类胡萝卜素的方法 |
| US8846374B2 (en) * | 2006-12-12 | 2014-09-30 | E I Du Pont De Nemours And Company | Carotenoid production in a recombinant oleaginous yeast |
-
2012
- 2012-08-07 HK HK14112719.9A patent/HK1199286A1/xx unknown
- 2012-08-07 WO PCT/IB2012/001513 patent/WO2013021261A2/en not_active Ceased
- 2012-08-07 RU RU2014109010A patent/RU2676730C2/ru not_active IP Right Cessation
- 2012-08-07 KR KR1020177037475A patent/KR101983115B1/ko not_active Expired - Fee Related
- 2012-08-07 JP JP2014524458A patent/JP6126597B2/ja active Active
- 2012-08-07 US US14/237,542 patent/US20140248668A1/en not_active Abandoned
- 2012-08-07 IN IN1129CHN2014 patent/IN2014CN01129A/en unknown
- 2012-08-07 EP EP12768896.8A patent/EP2742131B1/en not_active Not-in-force
- 2012-08-07 KR KR1020147006130A patent/KR101815063B1/ko not_active Expired - Fee Related
- 2012-08-07 CA CA2843549A patent/CA2843549A1/en not_active Abandoned
- 2012-08-07 CN CN201280038874.5A patent/CN103764818B/zh not_active Expired - Fee Related
- 2012-08-07 AU AU2012293402A patent/AU2012293402B2/en not_active Ceased
- 2012-08-07 BR BR112014002924A patent/BR112014002924A2/pt not_active Application Discontinuation
-
2017
- 2017-05-08 US US15/589,269 patent/US20170306376A1/en not_active Abandoned
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2003108882A (ru) * | 2000-09-01 | 2004-12-10 | Е.И.Дюпон Де Немур Энд Компани | Продуцирование каротиноидов из одноуглеродного субстрата |
Non-Patent Citations (3)
| Title |
|---|
| CASTILLO R. et al. Implications of carotenoid biosynthetic genes in apocarotenoid formation during the stigma development of Crocus sativus and its closer relatives. PLANT PHYSIOLOGY, 2005. * |
| QIU D. et al. Improved protocol for Agrobacterium mediated transformation of tomato and production of transgenic plants containing carotenoid biosynthetic gene CsZCD. SCIENTIA HORTICULTURAE, 2007. * |
| QIU D. et al. Improved protocol for Agrobacterium mediated transformation of tomato and production of transgenic plants containing carotenoid biosynthetic gene CsZCD. SCIENTIA HORTICULTURAE, 2007. CASTILLO R. et al. Implications of carotenoid biosynthetic genes in apocarotenoid formation during the stigma development of Crocus sativus and its closer relatives. PLANT PHYSIOLOGY, 2005. * |
Also Published As
| Publication number | Publication date |
|---|---|
| KR101983115B1 (ko) | 2019-05-29 |
| KR101815063B1 (ko) | 2018-01-05 |
| KR20140057319A (ko) | 2014-05-12 |
| CN103764818B (zh) | 2017-05-31 |
| AU2012293402B2 (en) | 2017-08-03 |
| US20140248668A1 (en) | 2014-09-04 |
| JP6126597B2 (ja) | 2017-05-10 |
| HK1199286A1 (en) | 2015-06-26 |
| EP2742131A2 (en) | 2014-06-18 |
| WO2013021261A2 (en) | 2013-02-14 |
| RU2014109010A (ru) | 2015-09-20 |
| IN2014CN01129A (enExample) | 2015-04-10 |
| WO2013021261A3 (en) | 2013-05-02 |
| EP2742131B1 (en) | 2018-11-28 |
| JP2014524246A (ja) | 2014-09-22 |
| CA2843549A1 (en) | 2013-02-14 |
| CN103764818A (zh) | 2014-04-30 |
| US20170306376A1 (en) | 2017-10-26 |
| AU2012293402A1 (en) | 2014-02-06 |
| AU2012293402A2 (en) | 2014-03-13 |
| KR20180004306A (ko) | 2018-01-10 |
| BR112014002924A2 (pt) | 2018-10-09 |
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