AU2008240029B2 - Delta 9 elongases and their use in making polyunsaturated fatty acids - Google Patents

Delta 9 elongases and their use in making polyunsaturated fatty acids Download PDF

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Publication number
AU2008240029B2
AU2008240029B2 AU2008240029A AU2008240029A AU2008240029B2 AU 2008240029 B2 AU2008240029 B2 AU 2008240029B2 AU 2008240029 A AU2008240029 A AU 2008240029A AU 2008240029 A AU2008240029 A AU 2008240029A AU 2008240029 B2 AU2008240029 B2 AU 2008240029B2
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AU
Australia
Prior art keywords
seq
elongase
acid
host cell
sequence
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
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AU2008240029A
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English (en)
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AU2008240029A1 (en
Inventor
Howard Glenn Damude
Quinn Qun Zhu
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EIDP Inc
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EI Du Pont de Nemours and Co
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Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of AU2008240029A1 publication Critical patent/AU2008240029A1/en
Application granted granted Critical
Publication of AU2008240029B2 publication Critical patent/AU2008240029B2/en
Ceased legal-status Critical Current
Anticipated expiration legal-status Critical

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings or cooking oils
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS OR COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings or cooking oils
    • A23D9/007Other edible oils or fats, e.g. shortenings or cooking oils characterised by ingredients other than fatty acid triglycerides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12Fatty acids or derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Zoology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Animal Husbandry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2008240029A 2007-04-16 2008-04-16 Delta 9 elongases and their use in making polyunsaturated fatty acids Ceased AU2008240029B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US91192507P 2007-04-16 2007-04-16
US60/911,925 2007-04-16
PCT/US2008/060396 WO2008128241A1 (en) 2007-04-16 2008-04-16 Delta 9 elongases and their use in making polyunsaturated fatty acids

Publications (2)

Publication Number Publication Date
AU2008240029A1 AU2008240029A1 (en) 2008-10-23
AU2008240029B2 true AU2008240029B2 (en) 2013-05-02

Family

ID=39619289

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2008240029A Ceased AU2008240029B2 (en) 2007-04-16 2008-04-16 Delta 9 elongases and their use in making polyunsaturated fatty acids
AU2008240028A Active AU2008240028B2 (en) 2007-04-16 2008-04-16 Delta 9 elongases and their use in making polyunsaturated fatty acids

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2008240028A Active AU2008240028B2 (en) 2007-04-16 2008-04-16 Delta 9 elongases and their use in making polyunsaturated fatty acids

Country Status (9)

Country Link
US (5) US7794701B2 (https=)
EP (2) EP2134837B1 (https=)
JP (1) JP5501954B2 (https=)
CN (1) CN101848996B (https=)
AU (2) AU2008240029B2 (https=)
BR (1) BRPI0807291A2 (https=)
CA (2) CA2679485A1 (https=)
DK (1) DK2137304T3 (https=)
WO (2) WO2008128241A1 (https=)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1756280T3 (en) 2004-04-22 2015-02-02 Commw Scient Ind Res Org SYNTHESIS OF CHAIN, polyunsaturated fatty acids BY RECOMBINANT CELLS
CA3056110C (en) 2004-04-22 2020-07-14 Surinder Pal Singh Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
CN101578363A (zh) 2006-08-29 2009-11-11 联邦科学技术研究组织 脂肪酸的合成
US7794701B2 (en) * 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
WO2008146745A1 (ja) * 2007-05-25 2008-12-04 Suntory Holdings Limited 新規なリゾホスファチジン酸アシル基転移酵素遺伝子
US8168858B2 (en) 2008-06-20 2012-05-01 E. I. Du Pont De Nemours And Company Delta-9 fatty acid elongase genes and their use in making polyunsaturated fatty acids
NO2358882T3 (https=) 2008-11-18 2017-12-23
CN102257126B (zh) * 2008-12-18 2014-07-09 纳幕尔杜邦公司 减少发酵过程中的丙二酸副产物
EP2442666B1 (en) 2009-06-16 2015-04-08 E. I. du Pont de Nemours and Company High eicosapentaenoic acid oils from improved optimized strains of yarrowia lipolytica
US8188335B2 (en) * 2009-07-17 2012-05-29 Abbott Laboratories Δ9-elongase for production of polyunsaturated fatty acid-enriched oils
ES2750241T3 (es) 2009-11-03 2020-03-25 Dsm Ip Assets Bv Aceite vegetal que comprende un ácido graso poliinsaturado que tiene al menos 20 átomos de carbono
DK2496092T3 (en) 2009-11-03 2018-07-16 Dsm Ip Assets Bv COMPOSITION WITH CELLS AND A POLYUM Saturated FAT ACID WITH AT LEAST 20 CARBON ATOMS (LC-PUFA)
WO2011133610A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
EP2609203B1 (en) 2010-08-26 2018-06-20 E. I. du Pont de Nemours and Company Recombinant microbial host cells for high eicosapentaenoic acid production
AU2011293167B2 (en) * 2010-08-26 2016-12-08 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
CA2808002A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
JP2014503216A (ja) 2010-12-30 2014-02-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー スクロース利用のための、ヤロウィア・リポリティカ(Yarrowialipolytica)におけるサッカロミセス・セレビシア(Saccharomycescerevisiae)SUC2遺伝子の使用
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
PL2861059T3 (pl) 2012-06-15 2017-10-31 Commw Scient Ind Res Org Wytwarzanie długołańcuchowych wielonienasyconych kwasów tłuszczowych w komórkach roślinnych
EP2935591B1 (en) 2012-12-21 2017-07-05 E. I. du Pont de Nemours and Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
CA2923296A1 (en) 2013-10-25 2015-04-30 Pioneer Hi-Bred International, Inc. Stem canker tolerant soybeans and methods of use
NZ721036A (en) 2013-12-18 2023-07-28 Grains Res & Dev Corp Lipid comprising long chain polyunsaturated fatty acids
PH12016502586B1 (en) 2014-06-27 2023-07-19 Commw Scient Ind Res Org Lipid comprising docosapentaenoic acid
CN106916796B (zh) * 2015-12-28 2021-06-29 丰益(上海)生物技术研发中心有限公司 裂殖壶菌delta-12脂肪酸脱饱和酶相关序列及其应用
CA3004914A1 (en) 2016-02-05 2017-08-10 Pioneer Hi-Bred International, Inc. Genetic loci associated with brown stem rot resistance in soybean and methods of use
EP3554265A1 (en) 2016-12-15 2019-10-23 DSM IP Assets B.V. Blend formulation comprising silicate and microbial and / or plant cells comprising a polyunsaturated fatty acid having at least 20 carbon atoms (lc-pufa)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002077213A2 (en) * 2001-03-26 2002-10-03 University Of Bristol New elongase gene and production of δ9-polyunsaturated fatty acids
US20050287652A1 (en) * 2004-06-25 2005-12-29 Damude Howard G Delta-8 desaturase and its use in making polyunsaturated fatty acids
WO2007061845A2 (en) * 2005-11-23 2007-05-31 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
WO2007123999A2 (en) * 2006-04-20 2007-11-01 E. I. Du Pont De Nemours And Company Δ17 desaturases and their use in making polyunsaturated fatty acids

Family Cites Families (49)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4670285A (en) 1982-08-06 1987-06-02 The University Of Toronto Innovations Foundation Infant formula
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5188958A (en) 1986-05-29 1993-02-23 Calgene, Inc. Transformation and foreign gene expression in brassica species
US5004863B2 (en) 1986-12-03 2000-10-17 Agracetus Genetic engineering of cotton plants and lines
JP2527340B2 (ja) 1986-12-15 1996-08-21 アプライド バイオシステムズ インコーポレイテッド ロ―ダミン染料の5−及び6−スクシニミジルカルボキシレ―ト異性体
US5416011A (en) 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US5366860A (en) 1989-09-29 1994-11-22 Applied Biosystems, Inc. Spectrally resolvable rhodamine dyes for nucleic acid sequence determination
WO1992017598A1 (en) 1991-03-29 1992-10-15 The Board Of Trustees Of The University Of Illinois Production fo transgenic soybean plants
US6372965B1 (en) 1992-11-17 2002-04-16 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants
US5648564A (en) 1995-12-21 1997-07-15 Kemin Industries, Inc. Process for the formation, isolation and purification of comestible xanthophyll crystals from plants
US5968809A (en) 1997-04-11 1999-10-19 Abbot Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6051754A (en) 1997-04-11 2000-04-18 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
NZ337459A (en) 1997-04-11 2000-07-28 Abbott Lab Nucleic acid construct in plants and dietary supplement
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US6432684B1 (en) 1997-04-11 2002-08-13 Abbott Laboratories Human desaturase gene and uses thereof
US6075183A (en) 1997-04-11 2000-06-13 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids in plants
US6403349B1 (en) 1998-09-02 2002-06-11 Abbott Laboratories Elongase gene and uses thereof
US6825017B1 (en) 1998-12-07 2004-11-30 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
CA2791961A1 (en) 1998-12-07 2000-06-15 Washington State University Research Foundation Desaturases and methods of using them for synthesis of polyunsaturated fatty acids
JP2004512020A (ja) 2000-06-23 2004-04-22 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 組換え構築物ならびにこれを遺伝子発現を減少させる際に用いる方法
DE60127967T2 (de) 2000-07-21 2008-01-17 E.I. Du Pont De Nemours And Co., Wilmington Ein mit der synthese von delta-12 epoxy-gruppen in fettsäuren von pflanzen assoziiertes cytochrom p450 enzym
CA2441265A1 (en) * 2001-03-16 2002-09-26 Basf Plant Science Gmbh Sugar and lipid metabolism regulators in plants
TWI324181B (en) 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US6797303B2 (en) 2001-09-04 2004-09-28 Lycored Natural Products Industries Ltd. Carotenoid extraction process
CA2510462A1 (en) * 2002-12-19 2004-07-08 University Of Bristol Method for producing polyunsaturated fatty acids in a transgenic plant or microorganism comprising an introduced delta-9-elongase and a delta-8-desaturase
US20040172682A1 (en) 2003-02-12 2004-09-02 Kinney Anthony J. Production of very long chain polyunsaturated fatty acids in oilseed plants
US7129089B2 (en) 2003-02-12 2006-10-31 E. I. Du Pont De Nemours And Company Annexin and P34 promoters and use in expression of transgenic genes in plants
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7125672B2 (en) 2003-05-07 2006-10-24 E. I. Du Pont De Nemours And Company Codon-optimized genes for the production of polyunsaturated fatty acids in oleaginous yeasts
US7259255B2 (en) 2003-06-25 2007-08-21 E. I. Du Pont De Nemours And Company Glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate mutase promoters for gene expression in oleaginous yeast
ES2713548T3 (es) 2003-08-01 2019-05-22 Basf Plant Science Gmbh Procedimiento para la producción de ácidos grasos poliinsaturados en organismos transgénicos
AU2004290051A1 (en) 2003-11-12 2005-05-26 E.I. Dupont De Nemours And Company Delta-15 desaturases suitable for altering levels of polyunsaturated fatty acids in oilseed plants and oleaginous yeast
WO2005049805A2 (en) 2003-11-14 2005-06-02 E.I. Dupont De Nemours And Company Fructose-bisphosphate aldolase regulatory sequences for gene expression in oleaginous yeast
CA2559360A1 (en) 2004-02-27 2005-09-09 Basf Plant Science Gmbh Method for producing c18, c20 and c22 polyunsaturated fatty acids in transgenic plants
DK1756280T3 (en) 2004-04-22 2015-02-02 Commw Scient Ind Res Org SYNTHESIS OF CHAIN, polyunsaturated fatty acids BY RECOMBINANT CELLS
CA3056110C (en) 2004-04-22 2020-07-14 Surinder Pal Singh Synthesis of long-chain polyunsaturated fatty acids by recombinant cells
US7465564B2 (en) 2004-08-10 2008-12-16 E.I. Du Pont De Nemours And Company Production of γ-linolenic acid in oleaginous yeast
US7550286B2 (en) 2004-11-04 2009-06-23 E. I. Du Pont De Nemours And Company Docosahexaenoic acid producing strains of Yarrowia lipolytica
US7943823B2 (en) 2006-04-28 2011-05-17 E.I. Du Pont De Nemours And Company Delta-8 desaturase and its use in making polyunsaturated fatty acids
US7678560B2 (en) 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US8389808B2 (en) 2007-02-12 2013-03-05 E.I. Du Pont De Nemours And Company Production of arachidonic acid in oilseed plants
ES2559312T3 (es) * 2007-04-03 2016-02-11 E. I. Du Pont De Nemours And Company Multienzimas y su uso en la fabricación de ácidos grasos poliinsaturados
US7790156B2 (en) * 2007-04-10 2010-09-07 E. I. Du Pont De Nemours And Company Δ-8 desaturases and their use in making polyunsaturated fatty acids
US7794701B2 (en) 2007-04-16 2010-09-14 E.I. Du Pont De Nemours And Company Δ-9 elongases and their use in making polyunsaturated fatty acids
US20090117253A1 (en) * 2007-10-03 2009-05-07 E. I. Du Pont De Nemours And Company Peroxisome biogenesis factor protein (pex) disruptions for altering polyunsaturated fatty acids and total lipid content in oleaginous eukaryotic organisms
CA2719361A1 (en) * 2008-04-03 2009-10-08 E.I. Du Pont De Nemours And Company Multizymes
JP5768056B2 (ja) 2009-10-30 2015-08-26 リコール メディカル インコーポレイテッドReCor Medical, Inc. 経皮的超音波腎神経除去による高血圧症を治療するための方法及び装置
SG11201608230PA (en) 2014-03-31 2016-10-28 Agency Science Tech & Res Image processing devices and image processing methods

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002077213A2 (en) * 2001-03-26 2002-10-03 University Of Bristol New elongase gene and production of δ9-polyunsaturated fatty acids
US20050287652A1 (en) * 2004-06-25 2005-12-29 Damude Howard G Delta-8 desaturase and its use in making polyunsaturated fatty acids
WO2007061845A2 (en) * 2005-11-23 2007-05-31 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
WO2007061742A1 (en) * 2005-11-23 2007-05-31 E. I. Du Pont De Nemours And Company Delta-9 elongases and their use in making polyunsaturated fatty acids
WO2007123999A2 (en) * 2006-04-20 2007-11-01 E. I. Du Pont De Nemours And Company Δ17 desaturases and their use in making polyunsaturated fatty acids

Also Published As

Publication number Publication date
US20080254195A1 (en) 2008-10-16
WO2008128240A1 (en) 2008-10-23
CN101848996B (zh) 2013-05-29
US8119860B2 (en) 2012-02-21
WO2008128241A1 (en) 2008-10-23
US8298797B2 (en) 2012-10-30
AU2008240028B2 (en) 2014-03-06
US20080254522A1 (en) 2008-10-16
CA2679485A1 (en) 2008-10-23
EP2137304B1 (en) 2013-08-14
US20120100595A1 (en) 2012-04-26
AU2008240028A1 (en) 2008-10-23
US8377673B2 (en) 2013-02-19
EP2134837A1 (en) 2009-12-23
CA2682962A1 (en) 2008-10-23
DK2137304T3 (da) 2013-11-11
JP2010524468A (ja) 2010-07-22
CN101848996A (zh) 2010-09-29
JP5501954B2 (ja) 2014-05-28
US20100162435A1 (en) 2010-06-24
AU2008240029A1 (en) 2008-10-23
US7794701B2 (en) 2010-09-14
US20100267098A1 (en) 2010-10-21
EP2134837B1 (en) 2014-01-01
EP2137304A1 (en) 2009-12-30
BRPI0807291A2 (pt) 2014-04-29

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