AU2004313913A1 - Methods for nucleic acid isolation and kits using a microfluidic device and concentration step - Google Patents
Methods for nucleic acid isolation and kits using a microfluidic device and concentration step Download PDFInfo
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- AU2004313913A1 AU2004313913A1 AU2004313913A AU2004313913A AU2004313913A1 AU 2004313913 A1 AU2004313913 A1 AU 2004313913A1 AU 2004313913 A AU2004313913 A AU 2004313913A AU 2004313913 A AU2004313913 A AU 2004313913A AU 2004313913 A1 AU2004313913 A1 AU 2004313913A1
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Classifications
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- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- B01L2300/0809—Geometry, shape and general structure rectangular shaped
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- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/06—Valves, specific forms thereof
- B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
- Y10T436/00—Chemistry: analytical and immunological testing
- Y10T436/25—Chemistry: analytical and immunological testing including sample preparation
Landscapes
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- Chemical Kinetics & Catalysis (AREA)
- Plant Pathology (AREA)
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- Proteomics, Peptides & Aminoacids (AREA)
- Clinical Laboratory Science (AREA)
- Immunology (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
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| US53252303P | 2003-12-24 | 2003-12-24 | |
| US60/532,523 | 2003-12-24 | ||
| US10/852,085 | 2004-05-24 | ||
| US10/852,085 US7939249B2 (en) | 2003-12-24 | 2004-05-24 | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
| PCT/US2004/035366 WO2005068627A1 (en) | 2003-12-24 | 2004-10-25 | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
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| AU2004313913A1 true AU2004313913A1 (en) | 2005-07-28 |
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| AU2004313913A Abandoned AU2004313913A1 (en) | 2003-12-24 | 2004-10-25 | Methods for nucleic acid isolation and kits using a microfluidic device and concentration step |
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| US (1) | US7939249B2 (enExample) |
| EP (2) | EP2305809A3 (enExample) |
| JP (1) | JP2007516723A (enExample) |
| AU (1) | AU2004313913A1 (enExample) |
| CA (1) | CA2551453A1 (enExample) |
| WO (1) | WO2005068627A1 (enExample) |
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| US6432290B1 (en) | 1999-11-26 | 2002-08-13 | The Governors Of The University Of Alberta | Apparatus and method for trapping bead based reagents within microfluidic analysis systems |
| CA2290731A1 (en) * | 1999-11-26 | 2001-05-26 | D. Jed Harrison | Apparatus and method for trapping bead based reagents within microfluidic analysis system |
| US6913697B2 (en) | 2001-02-14 | 2005-07-05 | Science & Technology Corporation @ Unm | Nanostructured separation and analysis devices for biological membranes |
| US7192560B2 (en) * | 2001-12-20 | 2007-03-20 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
| US6889468B2 (en) | 2001-12-28 | 2005-05-10 | 3M Innovative Properties Company | Modular systems and methods for using sample processing devices |
| JP2006501449A (ja) | 2002-09-27 | 2006-01-12 | ザ ジェネラル ホスピタル コーポレーション | 細胞分離のためのマイクロ流体デバイスおよびその使用 |
| CA2512071A1 (en) | 2002-12-30 | 2004-07-22 | The Regents Of The University Of California | Methods and apparatus for pathogen detection and analysis |
| US20050130177A1 (en) | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US7322254B2 (en) * | 2003-12-12 | 2008-01-29 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US20050142571A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using solid phase material |
| US20050142570A1 (en) * | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent |
| US7387877B2 (en) * | 2004-04-06 | 2008-06-17 | Oro Grande Technology | Bio-sensor and bio-sensor reporting system |
| US7799553B2 (en) | 2004-06-01 | 2010-09-21 | The Regents Of The University Of California | Microfabricated integrated DNA analysis system |
| WO2006032044A2 (en) * | 2004-09-15 | 2006-03-23 | Microchip Biotechnologies, Inc. | Microfluidic devices |
| JP2008538282A (ja) * | 2005-04-05 | 2008-10-23 | セルポイント ダイアグノスティクス, インコーポレイテッド | 装置および循環腫瘍細胞および他の粒子の濃縮および変更のための方法 |
| US20070196820A1 (en) | 2005-04-05 | 2007-08-23 | Ravi Kapur | Devices and methods for enrichment and alteration of cells and other particles |
| US7763210B2 (en) | 2005-07-05 | 2010-07-27 | 3M Innovative Properties Company | Compliant microfluidic sample processing disks |
| US7323660B2 (en) | 2005-07-05 | 2008-01-29 | 3M Innovative Properties Company | Modular sample processing apparatus kits and modules |
| US7754474B2 (en) | 2005-07-05 | 2010-07-13 | 3M Innovative Properties Company | Sample processing device compression systems and methods |
| US8921102B2 (en) | 2005-07-29 | 2014-12-30 | Gpb Scientific, Llc | Devices and methods for enrichment and alteration of circulating tumor cells and other particles |
| US20070059716A1 (en) * | 2005-09-15 | 2007-03-15 | Ulysses Balis | Methods for detecting fetal abnormality |
| CA2641271A1 (en) | 2006-02-03 | 2008-03-13 | Microchip Biotechnologies, Inc. | Microfluidic devices |
| US7766033B2 (en) | 2006-03-22 | 2010-08-03 | The Regents Of The University Of California | Multiplexed latching valves for microfluidic devices and processors |
| US20080050739A1 (en) | 2006-06-14 | 2008-02-28 | Roland Stoughton | Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats |
| EP2589668A1 (en) | 2006-06-14 | 2013-05-08 | Verinata Health, Inc | Rare cell analysis using sample splitting and DNA tags |
| US20080007838A1 (en) * | 2006-07-07 | 2008-01-10 | Omnitech Partners, Inc. | Field-of-view indicator, and optical system and associated method employing the same |
| US8841116B2 (en) | 2006-10-25 | 2014-09-23 | The Regents Of The University Of California | Inline-injection microdevice and microfabricated integrated DNA analysis system using same |
| US20080121591A1 (en) * | 2006-11-29 | 2008-05-29 | Canon U.S. Life Sciences, Inc. | Device for nucleic acid preparation |
| US20080131955A1 (en) * | 2006-11-30 | 2008-06-05 | Canon U.S. Life Sciences, Inc. | Method of Separating Target DNA from Mixed DNA |
| CA2671609A1 (en) | 2006-12-22 | 2008-07-03 | 3M Innovative Properties Company | Thermal transfer methods and structures for microfluidic systems |
| CN101568384B (zh) * | 2006-12-22 | 2013-05-01 | 3M创新有限公司 | 改进的样品处理装置、系统和方法 |
| EP2109666A4 (en) | 2007-02-05 | 2011-09-14 | Integenx Inc | MICROFLUIDIC AND NANOFLUIDIC DEVICES, SYSTEMS, AND APPLICATIONS |
| EP2425894B1 (en) | 2007-06-21 | 2016-12-28 | Gen-Probe Incorporated | Instruments and method for exposing a receptacle to multiple thermal zones |
| US8454906B2 (en) * | 2007-07-24 | 2013-06-04 | The Regents Of The University Of California | Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions |
| EP2234916A4 (en) | 2008-01-22 | 2016-08-10 | Integenx Inc | UNIVERSAL SPECIMEN PREPARATION SYSTEM AND ITS USE IN AN INTEGRATED ANALYSIS SYSTEM |
| US8304185B2 (en) | 2009-07-17 | 2012-11-06 | Canon U.S. Life Sciences, Inc. | Methods and systems for DNA isolation on a microfluidic device |
| JP5795255B2 (ja) | 2008-07-18 | 2015-10-14 | キヤノン ユー.エス. ライフ サイエンシズ, インコーポレイテッドCanon U.S. Life Sciences, Inc. | 微小流体dna試料調製のための方法およびシステム |
| US8672532B2 (en) * | 2008-12-31 | 2014-03-18 | Integenx Inc. | Microfluidic methods |
| US9447461B2 (en) | 2009-03-24 | 2016-09-20 | California Institute Of Technology | Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes |
| CN104722342B (zh) | 2009-03-24 | 2017-01-11 | 芝加哥大学 | 滑动式芯片装置和方法 |
| US9464319B2 (en) | 2009-03-24 | 2016-10-11 | California Institute Of Technology | Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes |
| US10196700B2 (en) | 2009-03-24 | 2019-02-05 | University Of Chicago | Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes |
| US8388908B2 (en) * | 2009-06-02 | 2013-03-05 | Integenx Inc. | Fluidic devices with diaphragm valves |
| EP2438016B1 (en) | 2009-06-05 | 2021-06-02 | IntegenX Inc. | Universal sample preparation system and use in an integrated analysis system |
| WO2011008217A1 (en) * | 2009-07-17 | 2011-01-20 | Canon U.S. Life Sciences, Inc. | Methods and systems for dna isolation on a microfluidic device |
| US8834792B2 (en) | 2009-11-13 | 2014-09-16 | 3M Innovative Properties Company | Systems for processing sample processing devices |
| USD667561S1 (en) | 2009-11-13 | 2012-09-18 | 3M Innovative Properties Company | Sample processing disk cover |
| USD638951S1 (en) | 2009-11-13 | 2011-05-31 | 3M Innovative Properties Company | Sample processing disk cover |
| USD638550S1 (en) | 2009-11-13 | 2011-05-24 | 3M Innovative Properties Company | Sample processing disk cover |
| US8584703B2 (en) | 2009-12-01 | 2013-11-19 | Integenx Inc. | Device with diaphragm valve |
| EP2536848B1 (en) * | 2010-02-15 | 2017-07-19 | Cascade Biosystems, Inc. | Methods and materials for detecting genetic or epigenetic elements |
| WO2011146596A1 (en) * | 2010-05-18 | 2011-11-24 | Cyvek, Inc. | Method and apparatus for performing extraction and enrichment of components in a biological sample |
| US8512538B2 (en) | 2010-05-28 | 2013-08-20 | Integenx Inc. | Capillary electrophoresis device |
| EP2606154B1 (en) | 2010-08-20 | 2019-09-25 | Integenx Inc. | Integrated analysis system |
| US8763642B2 (en) | 2010-08-20 | 2014-07-01 | Integenx Inc. | Microfluidic devices with mechanically-sealed diaphragm valves |
| ES2799422T3 (es) | 2011-03-10 | 2020-12-17 | General Atomics | Dispositivos y métodos de diagnóstico y preparación de muestras |
| EP2709761B1 (en) | 2011-05-18 | 2019-08-14 | DiaSorin S.p.A. | Systems and methods for volumetric metering on a sample processing device |
| USD672467S1 (en) | 2011-05-18 | 2012-12-11 | 3M Innovative Properties Company | Rotatable sample processing disk |
| AU2012255151B2 (en) | 2011-05-18 | 2015-09-03 | Diasorin Italia S.P.A. | Systems and methods for detecting the presence of a selected volume of material in a sample processing device |
| KR101963721B1 (ko) | 2011-05-18 | 2019-03-29 | 디아소린 에스.피.에이. | 샘플 처리 장치 상의 밸빙을 위한 시스템 및 방법 |
| US20150136604A1 (en) | 2011-10-21 | 2015-05-21 | Integenx Inc. | Sample preparation, processing and analysis systems |
| US10865440B2 (en) | 2011-10-21 | 2020-12-15 | IntegenX, Inc. | Sample preparation, processing and analysis systems |
| ES2665252T3 (es) | 2011-12-30 | 2018-04-25 | Abbott Molecular Inc. | Purificación de ácido nucleico de microorganismos a partir de muestras del hospedador |
| WO2014018195A1 (en) | 2012-06-21 | 2014-01-30 | Monsanto Technology Llc | Lysis buffer and methods for extraction of dna from plant material |
| US9399986B2 (en) | 2012-07-31 | 2016-07-26 | General Electric Company | Devices and systems for isolating biomolecules and associated methods thereof |
| WO2014031532A1 (en) * | 2012-08-19 | 2014-02-27 | University Of Rochester | Microfluidic device for filtering fluids and dialysis |
| US9707555B2 (en) | 2012-11-30 | 2017-07-18 | Qvella Corporation | Method for pretreatment of microbial samples |
| US9169521B1 (en) * | 2013-03-14 | 2015-10-27 | The Boeing Company | Point-of-collection sample preparation device and method |
| EP3030681A4 (en) * | 2013-08-07 | 2017-03-29 | Xagenic, Inc. | Systems, devices, and methods for deploying onboard reagents in a diagnostic device |
| CN110560187B (zh) | 2013-11-18 | 2022-01-11 | 尹特根埃克斯有限公司 | 用于样本分析的卡盒和仪器 |
| WO2015172255A1 (en) | 2014-05-16 | 2015-11-19 | Qvella Corporation | Apparatus, system and method for performing automated centrifugal separation |
| US10208332B2 (en) | 2014-05-21 | 2019-02-19 | Integenx Inc. | Fluidic cartridge with valve mechanism |
| CN107106983B (zh) | 2014-10-22 | 2021-04-16 | 尹特根埃克斯有限公司 | 用于样品制备、处理和分析的系统和方法 |
| US20200370036A1 (en) * | 2017-08-04 | 2020-11-26 | Microbedx, Inc. | Methods for Lysis of Cells Within a Sample |
| US20200263224A1 (en) * | 2017-08-18 | 2020-08-20 | Microbedx, Inc. | Methods for Antimicrobial Susceptibility Testing |
| WO2019222217A1 (en) * | 2018-05-14 | 2019-11-21 | Microbedx Inc. | Portable microfludic system for biological and analytical testing |
| CA3099674A1 (en) | 2018-05-25 | 2019-11-28 | Qvella Corporation | Methods and compositions for the selective lysis of blood cells and separation of microbial cells |
| IT201900008334A1 (it) | 2019-06-07 | 2020-12-07 | Nurex Srl | Dispositivo automatizzato per l'estrazione, purificazione e concentrazione degli acidi nucleici finalizzato a migliorare la sensibilità e la automazione della rilevazione di cellule nei campioni biologici |
| CN117255945A (zh) | 2021-03-31 | 2023-12-19 | 伊鲁米纳公司 | 纳米孔传感器装置 |
| US20240361297A1 (en) | 2021-11-08 | 2024-10-31 | Illumina, Inc. | Identifying nucleotides using changes in impedance between electrodes |
| CN114672541B (zh) * | 2022-03-21 | 2024-02-02 | 生工生物工程(上海)股份有限公司 | 一种核酸释放液、试剂盒及核酸释放的方法 |
| US20250205655A1 (en) | 2022-03-31 | 2025-06-26 | Illumina, Inc. | Amphiphilic polymers to be used in barriers and preparation thereof, barriers with nanopores and preparation thereof |
| US20230312857A1 (en) | 2022-03-31 | 2023-10-05 | Illumina Cambridge Limited | Nanopore devices including barriers using polymers with end groups, and methods of making the same |
| WO2023187104A1 (en) | 2022-03-31 | 2023-10-05 | Illumina Cambridge Limited | Nanopore devices including barriers using diblock or triblock copolymers, and methods of making the same |
| CA3244471A1 (en) | 2022-03-31 | 2023-10-05 | Illumina, Inc. | BARRIERS COMPRISING CROSS-CUT AMPHIPHILOUS MOLECULES, AND THEIR MANUFACTURE PROCESSES |
| US20230381718A1 (en) | 2022-03-31 | 2023-11-30 | Illumina Cambridge Limited | Barriers including molecules covalently bonded to amphiphilic molecules, and methods of making the same |
| WO2023187001A1 (en) | 2022-03-31 | 2023-10-05 | Illumina Cambridge Limited | Devices including osmotically balanced barriers, and methods of making and using the same |
| WO2023187081A1 (en) | 2022-03-31 | 2023-10-05 | Illumina Cambridge Limited | Methods for inserting nanopores into polymeric membranes using chaotropic solvents |
| US20240392117A1 (en) | 2023-05-25 | 2024-11-28 | Illumina, Inc. | Methods of making barriers including nanopores and crosslinked amphiphilic molecules, and barriers formed using same |
| WO2025106652A1 (en) | 2023-11-17 | 2025-05-22 | Illumina, Inc. | Fluidic devices, nanopore instruments, and methods |
Family Cites Families (204)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3157635A (en) | 1960-08-05 | 1964-11-17 | Takeda Chemical Industries Ltd | Sulfonic acid cation exchange resin purification of nucleotides |
| US4153661A (en) * | 1977-08-25 | 1979-05-08 | Minnesota Mining And Manufacturing Company | Method of making polytetrafluoroethylene composite sheet |
| US4399009A (en) | 1981-01-19 | 1983-08-16 | Oronzio Denora Impianti Elettrochimici S.P.A. | Electrolytic cell and method |
| US4399235A (en) | 1981-05-11 | 1983-08-16 | The Dow Chemical Company | High density ion exchange resins |
| US4565663A (en) * | 1981-06-26 | 1986-01-21 | Minnesota Mining And Manufacturing Company | Method for making water-swellable composite sheet |
| US4373519A (en) * | 1981-06-26 | 1983-02-15 | Minnesota Mining And Manufacturing Company | Composite wound dressing |
| US4460642A (en) | 1981-06-26 | 1984-07-17 | Minnesota Mining And Manufacturing Company | Water-swellable composite sheet of microfibers of PTFE and hydrophilic absorptive particles |
| US4483920A (en) | 1982-05-17 | 1984-11-20 | Hahnemann University | Immobilization of message RNA directly from cells onto filter material |
| US4539256A (en) | 1982-09-09 | 1985-09-03 | Minnesota Mining And Manufacturing Co. | Microporous sheet material, method of making and articles made therewith |
| JPS5968344A (ja) | 1982-10-12 | 1984-04-18 | Agency Of Ind Science & Technol | 非対称機能膜及びその製法 |
| US4780367A (en) | 1983-06-27 | 1988-10-25 | Minnesota Mining And Manufacturing Company | Tackified star block copolymer pressure-sensitive adhesive composition and the sheet materials coated therewith |
| US4839296A (en) * | 1985-10-18 | 1989-06-13 | Chem-Elec, Inc. | Blood plasma test method |
| US4757014A (en) | 1985-11-08 | 1988-07-12 | Minnesota Mining And Manufacturing Company | Immobilization of biologically active protein on a polymeric fibrous support |
| US4849311A (en) | 1986-09-24 | 1989-07-18 | Toa Nenryo Kogyo Kabushiki Kaisha | Immobilized electrolyte membrane |
| US4726989A (en) * | 1986-12-11 | 1988-02-23 | Minnesota Mining And Manufacturing | Microporous materials incorporating a nucleating agent and methods for making same |
| US5079155A (en) * | 1987-03-02 | 1992-01-07 | E. I. Du Pont De Nemours And Company | Fluorocarbon polymer support for chromatographic separations, diagnostic assays and enzyme immobilization |
| US4954444A (en) | 1987-03-02 | 1990-09-04 | E. I. Du Pont De Nemours And Company | Enzyme immobilization and bioaffinity separations with perfluorocarbon polymer-based supports |
| US4737560A (en) * | 1987-03-13 | 1988-04-12 | Minnesota Mining And Manufacturing Company | Polymer beads |
| US4906378A (en) * | 1987-12-28 | 1990-03-06 | Minnesota Mining And Manufacturing Company | Composite chromatographic article |
| US4810381A (en) * | 1987-12-28 | 1989-03-07 | Minnesota Mining And Manufacturing Company | Composite chromatographic article |
| US4971736A (en) | 1987-12-28 | 1990-11-20 | Minnesota Mining And Manufacturing Company | Method of preparing composite chromatographic article |
| US4923978A (en) * | 1987-12-28 | 1990-05-08 | E. I. Du Pont De Nemours & Company | Process for purifying nucleic acids |
| US5015373A (en) * | 1988-02-03 | 1991-05-14 | Regents Of The University Of Minnesota | High stability porous zirconium oxide spherules |
| US5205929A (en) * | 1988-02-03 | 1993-04-27 | Regents Of The University Of Minnesota | High stability porous zirconium oxide spherules |
| US5141634A (en) | 1988-02-03 | 1992-08-25 | Regents Of The University Of Minnesota | High stability porous zirconium oxide spherules |
| US5011861A (en) * | 1988-06-28 | 1991-04-30 | Millipore Corporation | Membranes for solid phase protein sequencing |
| US5182377A (en) * | 1988-09-09 | 1993-01-26 | Hoffmann-La Roche Inc. | Probes for detection of human papillomavirus |
| DE3921498A1 (de) | 1988-09-28 | 1990-03-29 | Bayer Ag | Polymer-gebundene-farbstoffe, verfahren zu deren herstellung und verwendung |
| US4957943A (en) | 1988-10-14 | 1990-09-18 | Minnesota Mining And Manufacturing Company | Particle-filled microporous materials |
| US5512439A (en) * | 1988-11-21 | 1996-04-30 | Dynal As | Oligonucleotide-linked magnetic particles and uses thereof |
| AU5188190A (en) | 1989-02-06 | 1990-08-24 | Gene-Trak Systems | Probes and methods for the detection of listeria |
| US5182083A (en) * | 1989-03-13 | 1993-01-26 | Beckman Instruments, Inc. | Sample wheel for chemistry analyzers |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| US5234809A (en) | 1989-03-23 | 1993-08-10 | Akzo N.V. | Process for isolating nucleic acid |
| US5010183A (en) * | 1989-07-07 | 1991-04-23 | Macfarlane Donald E | Process for purifying DNA and RNA using cationic detergents |
| EP0409432A3 (en) | 1989-07-20 | 1991-12-11 | Warner-Lambert Company | Confectionery delivery system |
| US5231015A (en) | 1989-10-18 | 1993-07-27 | Eastman Kodak Company | Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme |
| JP2978187B2 (ja) | 1989-11-02 | 1999-11-15 | 日本ケミカルリサーチ株式会社 | 修飾スーパーオキサイドディスムターゼの製造法 |
| US5187066A (en) * | 1990-02-14 | 1993-02-16 | Syntex (U.S.A.) Inc. | Methods for detecting amphiphilic antigens |
| US5071610A (en) | 1990-02-23 | 1991-12-10 | Minnesota Mining And Manufacturing Company | Method of making a controlled pore composite polytetrafluoroethylene |
| US5207915A (en) * | 1990-02-23 | 1993-05-04 | Minnesota Mining And Manufacturing Company | Separation method using controlled pore composite polytetrafluoroethylene article |
| US5147539A (en) | 1990-02-23 | 1992-09-15 | Minnesota Mining And Manufacturing Company | Controlled pore composite polytetrafluoroethylene article |
| US5770029A (en) | 1996-07-30 | 1998-06-23 | Soane Biosciences | Integrated electrophoretic microdevices |
| EP0447362A1 (en) | 1990-03-13 | 1991-09-18 | Warner-Lambert Company | Improved ingestible anion exchange resin delivery system compositions containing adipic acid |
| US5182016A (en) * | 1990-03-22 | 1993-01-26 | Regents Of The University Of Minnesota | Polymer-coated carbon-clad inorganic oxide particles |
| US5254262A (en) | 1990-03-22 | 1993-10-19 | Regents Of The University Of Minnesota | Carbon-clad zirconium oxide particles |
| US5271833A (en) | 1990-03-22 | 1993-12-21 | Regents Of The University Of Minnesota | Polymer-coated carbon-clad inorganic oxide particles |
| US5108597A (en) * | 1990-03-22 | 1992-04-28 | Regents Of The University Of Minnesota | Carbon-clad zirconium oxide particles |
| US5019232A (en) * | 1990-06-01 | 1991-05-28 | Minnesota Mining And Manufacturing Company | Medium for electrophoresis |
| US5334316A (en) | 1990-10-10 | 1994-08-02 | Brigham Young University | Process of using polytetraalkylammonium and polytrialkylamine-containing ligands bonded to inorganic supports for removing and concentrating desired ions from solutions |
| US5200471A (en) * | 1990-11-05 | 1993-04-06 | Minnesota Mining And Manufacturing Company | Biomolecules covalently immobilized with a high bound specific biological activity and method of preparing same |
| US5187083A (en) * | 1990-11-13 | 1993-02-16 | Specialty Laboratories, Inc. | Rapid purification of DNA |
| US5620852A (en) * | 1990-11-14 | 1997-04-15 | Hri Research, Inc. | Nucleic acid preparation methods |
| US5284940A (en) * | 1990-11-14 | 1994-02-08 | Hri Research, Inc. | Preparation for nucleic acid samples |
| US5294668A (en) * | 1990-11-15 | 1994-03-15 | Minnesota Mining And Manufacturing Company | Polyolefin pressure-sensitive adhesive compositions containing macromonomers |
| CA2059398C (en) * | 1991-02-07 | 1999-05-25 | Craig G. Markell | Solid phase extraction medium |
| USRE36811E (en) | 1991-02-07 | 2000-08-08 | Minnesota Mining And Manufacturing Co. | Solid phase extraction medium |
| US5264184A (en) | 1991-03-19 | 1993-11-23 | Minnesota Mining And Manufacturing Company | Device and a method for separating liquid samples |
| AU1664092A (en) | 1991-03-21 | 1992-10-21 | Eastman Kodak Company | Element and method for nucleic acid amplification and detection using adhered probes |
| WO1992018514A1 (en) | 1991-04-12 | 1992-10-29 | Minnesota Mining And Manufacturing Company | Purification of nucleic acids using metal oxide supports |
| CA2067711C (en) * | 1991-05-03 | 2000-08-08 | Daniel Lee Woodard | Solid phase extraction purification of dna |
| US5238621A (en) | 1991-06-28 | 1993-08-24 | Minnesota Mining And Manufacturing Company | Method of controlling porosity in a composite article |
| CA2112373C (en) | 1991-07-11 | 2010-04-20 | Timothy A. Holton | Genetic sequences encoding flavonoid pathway enzymes and uses therefor |
| FR2679255B1 (fr) * | 1991-07-17 | 1993-10-22 | Bio Merieux | Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation. |
| US6093558A (en) | 1991-07-25 | 2000-07-25 | Edge Biosystems, Inc. | Binding protein of biologically active compositions to an adhesive formulation on a substrate |
| US5993935A (en) | 1991-10-11 | 1999-11-30 | 3M Innovative Properties Company | Covalently reactive particles incorporated in a continous porous matrix |
| US5328758A (en) * | 1991-10-11 | 1994-07-12 | Minnesota Mining And Manufacturing Company | Particle-loaded nonwoven fibrous article for separations and purifications |
| US5380901A (en) * | 1992-01-30 | 1995-01-10 | The United States Of America As Represented By The Secretary Of Commerce | Multifunctional acrylates and the synthesis thereof |
| US5438128A (en) | 1992-02-07 | 1995-08-01 | Millipore Corporation | Method for rapid purifiction of nucleic acids using layered ion-exchange membranes |
| US5183705A (en) * | 1992-02-28 | 1993-02-02 | Minnesota Mining And Manufacturing Company | Pressure-sensitive adhesive composition having high shear strength |
| CA2096193A1 (en) | 1992-06-01 | 1993-12-02 | Daniel L. Woodard | Chemically synthesized silanes that bind nucleic acids |
| KR950701979A (ko) * | 1992-06-08 | 1995-05-17 | 다니엘 엘. 캐시앙 | 단핵 세포로부터 핵산의 조제 방법(Preparation of Nucleic Acid from Mononuclear Cells) |
| US5344701A (en) | 1992-06-09 | 1994-09-06 | Minnesota Mining And Manufacturing Company | Porous supports having azlactone-functional surfaces |
| JPH07508518A (ja) | 1992-06-23 | 1995-09-21 | ミネソタ マイニング アンド マニュファクチャリング カンパニー | アズラクトン官能支持体による脱タンパク質 |
| EP0665867B1 (en) * | 1992-10-21 | 1998-01-07 | Cornell Research Foundation, Inc. | Pore-size selective chemical modification of porous materials |
| CA2102264C (en) * | 1992-11-13 | 2000-08-01 | Daniel Lee Woodard | Boron silicates, aluminum silicates, phosphosilicates and purification of dna |
| AT398973B (de) | 1992-11-18 | 1995-02-27 | Bonn Guenther Dr | Verfahren zur trennung von nukleinsäuren |
| US5637687A (en) * | 1993-08-31 | 1997-06-10 | Wiggins; James C. | Methods and compositions for isolating nucleic acids |
| US5408002A (en) * | 1993-09-09 | 1995-04-18 | Minnesota Mining And Manufacturing Company | Azlactone-functional polymer blends, articles produced therefrom and methods for preparing both |
| US5438129A (en) | 1993-09-27 | 1995-08-01 | Becton Dickinson And Company | DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant |
| US5438127A (en) | 1993-09-27 | 1995-08-01 | Becton Dickinson And Company | DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane |
| AU693836B2 (en) | 1993-11-29 | 1998-07-09 | Gen-Probe Incorporated | Method for extracting nucleic acids from a wide range of organisms |
| US5610287A (en) * | 1993-12-06 | 1997-03-11 | Molecular Tool, Inc. | Method for immobilizing nucleic acid molecules |
| DE69412286T2 (de) | 1993-12-22 | 1998-12-03 | Minnesota Mining And Mfg. Co., Saint Paul, Minn. | Verwendung von blattförmigen materialien für festphasenextraktionen und festphasenreaktionen |
| US6207251B1 (en) * | 1994-01-10 | 2001-03-27 | Minnesota Mining And Manufacturing Company | Reinforced particle-loaded fibrillated PTFE web |
| US6780818B2 (en) | 1994-02-02 | 2004-08-24 | The Regents Of The University Of California | Quantitative organic vapor-particle sampler |
| DE69518743T2 (de) | 1994-03-08 | 2000-12-28 | Amersham Pharmacia Biotech Uk Ltd., Little Chalfont | Modifizierung von nukleotidanalogen |
| US5529686A (en) * | 1994-07-15 | 1996-06-25 | Minnesota Mining And Manufacturing Company | Composite membranes for solid phase extractions and reactions |
| US5625053A (en) | 1994-08-26 | 1997-04-29 | Board Of Regents For Northern Illinois Univ. | Method of isolating purified plasmid DNA using a nonionic detergent, solution |
| SE9500183D0 (sv) | 1995-01-20 | 1995-01-20 | Pharmacia Biotech Ab | Method for the purifivation of short nucleic acids |
| US5472600A (en) | 1995-02-01 | 1995-12-05 | Minnesota Mining And Manufacturing Company | Gradient density filter |
| US6063838A (en) * | 1995-02-16 | 2000-05-16 | 3M Innovative Properties Company | Blended pressure-sensitive adhesives |
| US5705059A (en) | 1995-02-27 | 1998-01-06 | Miltenyi; Stefan | Magnetic separation apparatus |
| US5741828A (en) * | 1995-05-01 | 1998-04-21 | S.K.Y. Polymers, Inc. | Flexible hydrophilic composite coatings |
| US5709943A (en) * | 1995-05-04 | 1998-01-20 | Minnesota Mining And Manufacturing Company | Biological adsorption supports |
| US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| US6168948B1 (en) * | 1995-06-29 | 2001-01-02 | Affymetrix, Inc. | Miniaturized genetic analysis systems and methods |
| US5700902A (en) | 1995-07-27 | 1997-12-23 | Circe Biomedical, Inc. | Block copolymers |
| DE19530132C2 (de) | 1995-08-16 | 1998-07-16 | Max Planck Gesellschaft | Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien |
| US5804684A (en) | 1995-08-24 | 1998-09-08 | The Theobald Smith Research Institute, Inc. | Method for isolating nucleic acids |
| US5620869A (en) | 1995-09-28 | 1997-04-15 | Becton, Dickinson And Company | Methods for reducing inhibition of nucleic acid amplification reactions |
| US20010055812A1 (en) | 1995-12-05 | 2001-12-27 | Alec Mian | Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics |
| US6068751A (en) * | 1995-12-18 | 2000-05-30 | Neukermans; Armand P. | Microfluidic valve and integrated microfluidic system |
| JP3818689B2 (ja) * | 1996-01-16 | 2006-09-06 | 富士写真フイルム株式会社 | コロイド状シリカをコアとし、有機ポリマーをシェルとするコア/シェル状複合粒子の水性分散物及びその製造方法 |
| ATE191515T1 (de) | 1996-01-23 | 2000-04-15 | Rapigene Inc | Verfahren und zusammensetzungen zum analysieren von nukleinsäuremolekulen mittels bemessungstechniken |
| FR2744803B1 (fr) * | 1996-02-12 | 1998-03-13 | Bio Merieux | Procede et dispositif de traitement d'une carte d'analyse |
| US5904848A (en) * | 1996-02-21 | 1999-05-18 | Cpg, Inc. | Controlled pore glass-synthetic resin membrane |
| US5837203A (en) | 1996-04-09 | 1998-11-17 | Sievers Instruments, Inc. | Device to alternately supply a fluid to an analyzer |
| US5882521A (en) | 1996-04-18 | 1999-03-16 | Waters Investment Ltd. | Water-wettable chromatographic media for solid phase extraction |
| US6074827A (en) * | 1996-07-30 | 2000-06-13 | Aclara Biosciences, Inc. | Microfluidic method for nucleic acid purification and processing |
| US6143248A (en) | 1996-08-12 | 2000-11-07 | Gamera Bioscience Corp. | Capillary microvalve |
| DE19731670C2 (de) | 1997-07-23 | 2000-06-29 | Dorothea Waschk | Verfahren zur Reinigung und gegebenenfalls Analyse von Nukleinsäuren aus biologischen Proben |
| AU4775497A (en) | 1996-09-19 | 1998-04-14 | W. Kurt Roth | Method for purifying and eventually analyzing nucleic acids from biological test samples |
| DK0951280T3 (da) * | 1996-10-03 | 2004-05-17 | Hermes Biosciences Inc | Hydrofile mikropartikler og fremgangsmåder til fremstilling heraf |
| US6093559A (en) | 1996-10-24 | 2000-07-25 | Corning Incorporated | Producing low binding hydrophobic surfaces by treating with a low HLB number non-ionic surfactant |
| US5786219A (en) * | 1996-10-28 | 1998-07-28 | Molecular Probes, Inc. | Microspheres with fluorescent spherical zones |
| EP0946250B1 (en) | 1996-11-12 | 2004-06-30 | Whatman Inc. | Hydrophilic polymeric phase inversion membrane |
| AU5895898A (en) | 1996-12-20 | 1998-07-17 | Gamera Bioscience Corporation | An affinity binding-based system for detecting particulates in a fluid |
| SE9700383D0 (sv) | 1997-02-04 | 1997-02-04 | Pharmacia Biotech Ab | An adsorption/separation method and a medium for adsorption/separation |
| US6048457A (en) * | 1997-02-26 | 2000-04-11 | Millipore Corporation | Cast membrane structures for sample preparation |
| US5997818A (en) | 1997-02-27 | 1999-12-07 | Minnesota Mining And Manufacturing Company | Cassette for tonometric calibration |
| SE9700769D0 (sv) | 1997-03-04 | 1997-03-04 | Pharmacia Biotech Ab | Matriser för separation och separation som utnyttjar matriserna |
| US5999935A (en) | 1997-03-28 | 1999-12-07 | International Business Machines Corporation | Tail compression of a sparse log stream of a multisystem environment |
| US6632399B1 (en) | 1998-05-22 | 2003-10-14 | Tecan Trading Ag | Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays |
| US6063589A (en) | 1997-05-23 | 2000-05-16 | Gamera Bioscience Corporation | Devices and methods for using centripetal acceleration to drive fluid movement on a microfluidics system |
| US5919626A (en) | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| US6348336B1 (en) * | 1997-07-01 | 2002-02-19 | Alpha Therapeutic Corporation | Process for purification of PCR test samples |
| EP0897978A3 (en) | 1997-08-22 | 2001-10-17 | Becton, Dickinson and Company | Zirconium oxide and related compounds for purification of nucleic acids |
| US6451260B1 (en) | 1997-08-26 | 2002-09-17 | Dyax Corp. | Method for producing microporous elements, the microporous elements thus produced and uses thereof |
| WO1999015876A1 (en) | 1997-09-19 | 1999-04-01 | Aclara Biosciences, Inc. | Apparatus and method for transferring liquids |
| AU753307B2 (en) | 1997-09-19 | 2002-10-17 | Aclara Biosciences, Inc. | Capillary electroflow apparatus and method |
| DE19746874A1 (de) | 1997-10-23 | 1999-04-29 | Qiagen Gmbh | Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen |
| FI974124A0 (fi) | 1997-11-04 | 1997-11-04 | Satu Aokerman | Foerfarande foer separering av proteinfria aemnen fraon proteinhaltiga aemnen foer daerpao foeljande behandling |
| US6241980B1 (en) | 1997-11-04 | 2001-06-05 | Becton, Dickinson And Company | Sample processing method using ion exchange resin |
| US6475722B1 (en) | 1997-12-03 | 2002-11-05 | Curagen Corporation | Surface treatments for DNA processing devices |
| JP4209589B2 (ja) | 1997-12-24 | 2009-01-14 | シーフィード | 一体型流体操作カートリッジ |
| AU2347799A (en) | 1998-01-29 | 1999-08-16 | University Of Pittsburgh | Thermal expansion-induced fluid control for microfluidic devices |
| EP1062510A2 (en) | 1998-03-10 | 2000-12-27 | Strategic Diagnostics Inc. | Integrated assay device and methods of production and use |
| US6265168B1 (en) | 1998-10-06 | 2001-07-24 | Transgenomic, Inc. | Apparatus and method for separating and purifying polynucleotides |
| US6534262B1 (en) | 1998-05-14 | 2003-03-18 | Whitehead Institute For Biomedical Research | Solid phase technique for selectively isolating nucleic acids |
| US6074927A (en) | 1998-06-01 | 2000-06-13 | Advanced Micro Devices, Inc. | Shallow trench isolation formation with trench wall spacer |
| US6465225B1 (en) | 1998-06-29 | 2002-10-15 | Evotec Oai Ag | Method and device for manipulating particles in microsystems |
| US6103199A (en) | 1998-09-15 | 2000-08-15 | Aclara Biosciences, Inc. | Capillary electroflow apparatus and method |
| US6572830B1 (en) * | 1998-10-09 | 2003-06-03 | Motorola, Inc. | Integrated multilayered microfludic devices and methods for making the same |
| US6277488B1 (en) | 1998-10-28 | 2001-08-21 | 3M Innovative Properties Company | Adhesive composition containing a block copolymer composition and polyphenylene oxide resin and products thereof |
| US6240790B1 (en) | 1998-11-09 | 2001-06-05 | Agilent Technologies, Inc. | Device for high throughout sample processing, analysis and collection, and methods of use thereof |
| WO2000045180A1 (en) | 1999-02-01 | 2000-08-03 | 3M Innovative Properties Company | Poly(alpha-olefin) adhesive cover tapes for analytical receptacles |
| AU3157800A (en) * | 1999-02-22 | 2000-09-14 | Evotec Biosystems Ag | Utilization of supporting material in capillary electrochromatography |
| US6479300B1 (en) | 1999-03-15 | 2002-11-12 | Millipore Corporation | Metal loaded ligand bound membranes for metal ion affinity chromatography |
| US6306273B1 (en) | 1999-04-13 | 2001-10-23 | Aclara Biosciences, Inc. | Methods and compositions for conducting processes in microfluidic devices |
| JP2002544320A (ja) | 1999-05-05 | 2002-12-24 | スリーエム イノベイティブ プロパティズ カンパニー | シリコーン接着剤、製品、および方法 |
| DE60012562D1 (de) | 1999-06-18 | 2004-09-02 | Gamera Bioscience Corp | Vorrichtungen und verfahren zur durchführung miniaturisierter homogener tests |
| US6664104B2 (en) | 1999-06-25 | 2003-12-16 | Cepheid | Device incorporating a microfluidic chip for separating analyte from a sample |
| GB9915398D0 (en) | 1999-07-02 | 1999-09-01 | Baker Matthew J | Magnetic particles |
| US6383783B1 (en) | 1999-09-21 | 2002-05-07 | 3M Innovative Properties Company | Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant |
| US6414136B1 (en) | 1999-10-06 | 2002-07-02 | Prolinx, Inc. | Removal of dye-labeled dideoxy terminators from DNA sequencing reactions |
| AU1440901A (en) * | 1999-10-27 | 2001-05-08 | 3M Innovative Properties Company | Fluorochemical sulfonamide surfactants |
| ES2256068T3 (es) | 1999-11-17 | 2006-07-16 | Roche Diagnostics Gmbh | Particulas de cristal magneticas, metodo para su preparacion y usos de las mismas. |
| GB9927904D0 (en) | 1999-11-25 | 2000-01-26 | Amersham Pharm Biotech Ab | A method fro obtaining a nucleic acid variant |
| CA2290731A1 (en) | 1999-11-26 | 2001-05-26 | D. Jed Harrison | Apparatus and method for trapping bead based reagents within microfluidic analysis system |
| US6692596B2 (en) * | 1999-12-23 | 2004-02-17 | 3M Innovative Properties Company | Micro-titer plate and method of making same |
| US7311880B2 (en) | 1999-12-23 | 2007-12-25 | 3M Innovative Properties Company | Well-less filtration device |
| US6875348B2 (en) * | 2000-02-18 | 2005-04-05 | The Board Of Trustees Of The Leland Stanford Junior University | Separation column having a photopolymerized sol-gel component and associated methods |
| WO2001062976A1 (en) | 2000-02-23 | 2001-08-30 | Wen Shao | Rapid nucleic acid separation, isolation and purification methods |
| US6720157B2 (en) * | 2000-02-23 | 2004-04-13 | Zyomyx, Inc. | Chips having elevated sample surfaces |
| EP1134586A1 (en) | 2000-03-08 | 2001-09-19 | Tibotec N.V. | Method for adding a fluid in a series of wells |
| WO2001068913A2 (en) | 2000-03-13 | 2001-09-20 | Genset | Nucleic acid detection method and system |
| EP1274503A2 (en) | 2000-03-14 | 2003-01-15 | Hammen Corporation | Composite matrices with interstitial polymer networks |
| WO2001071732A2 (en) | 2000-03-24 | 2001-09-27 | Qiagen Gmbh | Porous ferro- or ferrimagnetic glass particles for isolating molecules |
| US6818435B2 (en) * | 2000-05-15 | 2004-11-16 | Tecan Trading Ag | Microfluidics devices and methods for performing cell based assays |
| US6734401B2 (en) * | 2000-06-28 | 2004-05-11 | 3M Innovative Properties Company | Enhanced sample processing devices, systems and methods |
| US6720187B2 (en) * | 2000-06-28 | 2004-04-13 | 3M Innovative Properties Company | Multi-format sample processing devices |
| US6627159B1 (en) | 2000-06-28 | 2003-09-30 | 3M Innovative Properties Company | Centrifugal filling of sample processing devices |
| US6504021B2 (en) * | 2000-07-05 | 2003-01-07 | Edge Biosystems, Inc. | Ion exchange method for DNA purification |
| US6537502B1 (en) * | 2000-07-25 | 2003-03-25 | Harvard Apparatus, Inc. | Surface coated housing for sample preparation |
| US6503716B1 (en) | 2000-11-28 | 2003-01-07 | Pe Corporation (Ny) | Compositions and methods for extracting a nucleic acid |
| US6617136B2 (en) | 2001-04-24 | 2003-09-09 | 3M Innovative Properties Company | Biological sample processing methods and compositions that include surfactants |
| US20030017567A1 (en) * | 2001-04-24 | 2003-01-23 | 3M Innovative Properties Company | Biological sample processing methods and compositions that include surfactants |
| US7374724B2 (en) * | 2001-05-29 | 2008-05-20 | Tecan Trading Ag | Device for processing samples, use of the device, and method for producing the device |
| US7138436B2 (en) * | 2001-06-13 | 2006-11-21 | 3M Innovative Properties Company | Uncrosslinked foams made from emulsions |
| US6919058B2 (en) * | 2001-08-28 | 2005-07-19 | Gyros Ab | Retaining microfluidic microcavity and other microfluidic structures |
| US7189368B2 (en) * | 2001-09-17 | 2007-03-13 | Gyros Patent Ab | Functional unit enabling controlled flow in a microfluidic device |
| US7347976B2 (en) | 2001-12-20 | 2008-03-25 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix |
| US7192560B2 (en) | 2001-12-20 | 2007-03-20 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using anion exchange |
| US6532997B1 (en) | 2001-12-28 | 2003-03-18 | 3M Innovative Properties Company | Sample processing device with integral electrophoresis channels |
| WO2003059484A1 (fr) | 2001-12-28 | 2003-07-24 | Hitachi, Ltd. | Extracteur, analyseur chimique et procede d'analyse chimique |
| US6833238B2 (en) | 2002-01-04 | 2004-12-21 | Applera Corporation | Petal-array support for use with microplates |
| US6723236B2 (en) * | 2002-03-19 | 2004-04-20 | Waters Investments Limited | Device for solid phase extraction and method for purifying samples prior to analysis |
| JP2006505766A (ja) | 2002-04-11 | 2006-02-16 | バースタイン テクノロジーズ,インコーポレイティド | 分析ディスクを含むマルチパラメータ検定とその関連方法 |
| US6998271B2 (en) * | 2002-06-03 | 2006-02-14 | The Hong Kong Polytechnic University | Luminescent sensory material for organic-halogen compounds, and methods and apparatus utilizing such |
| US20040023220A1 (en) | 2002-07-23 | 2004-02-05 | Lawrence Greenfield | Integrated method for PCR cleanup and oligonucleotide removal |
| US7214348B2 (en) * | 2002-07-26 | 2007-05-08 | Applera Corporation | Microfluidic size-exclusion devices, systems, and methods |
| AU2003254174A1 (en) | 2002-07-26 | 2004-02-16 | Applera Corporation | Size-exclusion ion-exchange particles |
| AU2003252177A1 (en) | 2002-07-26 | 2004-02-16 | Applera Corporation | Microfluidic device including purification column with excess diluent, and method |
| US20040016702A1 (en) * | 2002-07-26 | 2004-01-29 | Applera Corporation | Device and method for purification of nucleic acids |
| EP1534430A4 (en) | 2002-07-26 | 2005-11-23 | Applera Corp | DEVICES, SYSTEMS AND METHODS FOR EXCLUSION OF MICROFLUIDIC SIZE |
| US7332348B2 (en) | 2003-02-28 | 2008-02-19 | Applera Corporation | Sample substrate having a divided sample chamber and method of loading thereof |
| US7981600B2 (en) | 2003-04-17 | 2011-07-19 | 3M Innovative Properties Company | Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene |
| US6883238B1 (en) * | 2003-09-08 | 2005-04-26 | Khiem Tran | Hairstyling scissors |
| US20050130177A1 (en) | 2003-12-12 | 2005-06-16 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US7322254B2 (en) * | 2003-12-12 | 2008-01-29 | 3M Innovative Properties Company | Variable valve apparatus and methods |
| US20050142570A1 (en) | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent |
| US20050142571A1 (en) | 2003-12-24 | 2005-06-30 | 3M Innovative Properties Company | Methods for nucleic acid isolation and kits using solid phase material |
| US7727710B2 (en) | 2003-12-24 | 2010-06-01 | 3M Innovative Properties Company | Materials, methods, and kits for reducing nonspecific binding of molecules to a surface |
-
2004
- 2004-05-24 US US10/852,085 patent/US7939249B2/en active Active
- 2004-10-25 EP EP10193349A patent/EP2305809A3/en not_active Withdrawn
- 2004-10-25 AU AU2004313913A patent/AU2004313913A1/en not_active Abandoned
- 2004-10-25 WO PCT/US2004/035366 patent/WO2005068627A1/en not_active Ceased
- 2004-10-25 CA CA002551453A patent/CA2551453A1/en not_active Abandoned
- 2004-10-25 EP EP04796358A patent/EP1697513A1/en not_active Ceased
- 2004-10-25 JP JP2006546979A patent/JP2007516723A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP2305809A2 (en) | 2011-04-06 |
| EP2305809A3 (en) | 2011-08-03 |
| US20050142663A1 (en) | 2005-06-30 |
| JP2007516723A (ja) | 2007-06-28 |
| US7939249B2 (en) | 2011-05-10 |
| WO2005068627A1 (en) | 2005-07-28 |
| EP1697513A1 (en) | 2006-09-06 |
| CA2551453A1 (en) | 2005-07-28 |
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