JP2007516723A - マイクロ流体装置および濃縮ステップを用いて核酸を単離する方法およびキット - Google Patents

マイクロ流体装置および濃縮ステップを用いて核酸を単離する方法およびキット Download PDF

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Publication number
JP2007516723A
JP2007516723A JP2006546979A JP2006546979A JP2007516723A JP 2007516723 A JP2007516723 A JP 2007516723A JP 2006546979 A JP2006546979 A JP 2006546979A JP 2006546979 A JP2006546979 A JP 2006546979A JP 2007516723 A JP2007516723 A JP 2007516723A
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sample
nucleic acid
inhibitor
chamber
valved
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JP2007516723A5 (enExample
Inventor
ブイ． パルササラシー，ランジャニ
ケー． エリクソン，カトヤ
ベディンガム，ウィリアム
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3M Innovative Properties Co
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3M Innovative Properties Co
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0677—Valves, specific forms thereof phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T436/00—Chemistry: analytical and immunological testing
    • Y10T436/25—Chemistry: analytical and immunological testing including sample preparation

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Dispersion Chemistry (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2006546979A 2003-12-24 2004-10-25 マイクロ流体装置および濃縮ステップを用いて核酸を単離する方法およびキット Withdrawn JP2007516723A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US53252303P 2003-12-24 2003-12-24
US10/852,085 US7939249B2 (en) 2003-12-24 2004-05-24 Methods for nucleic acid isolation and kits using a microfluidic device and concentration step
PCT/US2004/035366 WO2005068627A1 (en) 2003-12-24 2004-10-25 Methods for nucleic acid isolation and kits using a microfluidic device and concentration step

Publications (2)

Publication Number Publication Date
JP2007516723A true JP2007516723A (ja) 2007-06-28
JP2007516723A5 JP2007516723A5 (enExample) 2007-12-06

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US (1) US7939249B2 (enExample)
EP (2) EP1697513A1 (enExample)
JP (1) JP2007516723A (enExample)
AU (1) AU2004313913A1 (enExample)
CA (1) CA2551453A1 (enExample)
WO (1) WO2005068627A1 (enExample)

Families Citing this family (90)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6432290B1 (en) 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
CA2290731A1 (en) * 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US6913697B2 (en) 2001-02-14 2005-07-05 Science & Technology Corporation @ Unm Nanostructured separation and analysis devices for biological membranes
US7192560B2 (en) * 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
US6889468B2 (en) 2001-12-28 2005-05-10 3M Innovative Properties Company Modular systems and methods for using sample processing devices
EP1569510B1 (en) 2002-09-27 2011-11-02 The General Hospital Corporation Microfluidic device for cell separation and uses thereof
EP1594694A4 (en) * 2002-12-30 2010-01-20 Univ California METHOD AND DEVICE FOR DETECTING AND ANALYZING DISEASES OF DISEASE
US20050130177A1 (en) * 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7322254B2 (en) * 2003-12-12 2008-01-29 3M Innovative Properties Company Variable valve apparatus and methods
US20050142571A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US20050142570A1 (en) * 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent
US7387877B2 (en) * 2004-04-06 2008-06-17 Oro Grande Technology Bio-sensor and bio-sensor reporting system
US7799553B2 (en) 2004-06-01 2010-09-21 The Regents Of The University Of California Microfabricated integrated DNA analysis system
US7745207B2 (en) 2006-02-03 2010-06-29 IntegenX, Inc. Microfluidic devices
CN102759466A (zh) 2004-09-15 2012-10-31 英特基因有限公司 微流体装置
EP2594631A1 (en) * 2005-04-05 2013-05-22 Cellpoint Diagnostics Devices and method for detecting circulating tumor cells and other particles
US20070196820A1 (en) 2005-04-05 2007-08-23 Ravi Kapur Devices and methods for enrichment and alteration of cells and other particles
US7754474B2 (en) 2005-07-05 2010-07-13 3M Innovative Properties Company Sample processing device compression systems and methods
US7323660B2 (en) 2005-07-05 2008-01-29 3M Innovative Properties Company Modular sample processing apparatus kits and modules
US7763210B2 (en) * 2005-07-05 2010-07-27 3M Innovative Properties Company Compliant microfluidic sample processing disks
US8921102B2 (en) 2005-07-29 2014-12-30 Gpb Scientific, Llc Devices and methods for enrichment and alteration of circulating tumor cells and other particles
US20070059716A1 (en) * 2005-09-15 2007-03-15 Ulysses Balis Methods for detecting fetal abnormality
US7766033B2 (en) 2006-03-22 2010-08-03 The Regents Of The University Of California Multiplexed latching valves for microfluidic devices and processors
EP2589668A1 (en) 2006-06-14 2013-05-08 Verinata Health, Inc Rare cell analysis using sample splitting and DNA tags
US20080050739A1 (en) 2006-06-14 2008-02-28 Roland Stoughton Diagnosis of fetal abnormalities using polymorphisms including short tandem repeats
US20080007838A1 (en) * 2006-07-07 2008-01-10 Omnitech Partners, Inc. Field-of-view indicator, and optical system and associated method employing the same
WO2008052138A2 (en) 2006-10-25 2008-05-02 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated dna analysis system using same
US20080121591A1 (en) * 2006-11-29 2008-05-29 Canon U.S. Life Sciences, Inc. Device for nucleic acid preparation
US20080131955A1 (en) * 2006-11-30 2008-06-05 Canon U.S. Life Sciences, Inc. Method of Separating Target DNA from Mixed DNA
US8128893B2 (en) 2006-12-22 2012-03-06 3M Innovative Properties Company Thermal transfer methods and structures for microfluidic systems
CN101568384B (zh) * 2006-12-22 2013-05-01 3M创新有限公司 改进的样品处理装置、系统和方法
EP2109666A4 (en) 2007-02-05 2011-09-14 Integenx Inc MICROFLUIDIC AND NANOFLUIDIC DEVICES, SYSTEMS, AND APPLICATIONS
EP2384432B1 (en) * 2007-06-21 2016-12-28 Gen-Probe Incorporated Instrument and receptacles for performing processes
WO2009015296A1 (en) * 2007-07-24 2009-01-29 The Regents Of The University Of California Microfabricated dropley generator
CN101990516B (zh) 2008-01-22 2015-09-09 英特基因有限公司 多用试样准备系统及其在集成分析系统中的使用
EP2315848B1 (en) * 2008-07-18 2014-12-10 Canon U.S. Life Sciences, Inc. Methods and systems for microfluidic dna sample preparation
US8304185B2 (en) 2009-07-17 2012-11-06 Canon U.S. Life Sciences, Inc. Methods and systems for DNA isolation on a microfluidic device
EP2384429A1 (en) * 2008-12-31 2011-11-09 Integenx Inc. Instrument with microfluidic chip
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
EP2412020B1 (en) 2009-03-24 2020-09-30 University Of Chicago Slip chip device and methods
CN102459565A (zh) * 2009-06-02 2012-05-16 尹特根埃克斯有限公司 具有隔膜阀的流控设备
EP2438016B1 (en) 2009-06-05 2021-06-02 IntegenX Inc. Universal sample preparation system and use in an integrated analysis system
JP5815518B2 (ja) * 2009-07-17 2015-11-17 キヤノン ユー．エス． ライフ サイエンシズ， インコーポレイテッドＣａｎｏｎ Ｕ．Ｓ． Ｌｉｆｅ Ｓｃｉｅｎｃｅｓ， Ｉｎｃ． マイクロ流体装置においてｄｎａを単離する方法及びシステム
USD667561S1 (en) 2009-11-13 2012-09-18 3M Innovative Properties Company Sample processing disk cover
USD638951S1 (en) 2009-11-13 2011-05-31 3M Innovative Properties Company Sample processing disk cover
US8834792B2 (en) 2009-11-13 2014-09-16 3M Innovative Properties Company Systems for processing sample processing devices
USD638550S1 (en) 2009-11-13 2011-05-24 3M Innovative Properties Company Sample processing disk cover
US8584703B2 (en) 2009-12-01 2013-11-19 Integenx Inc. Device with diaphragm valve
US8551701B2 (en) * 2010-02-15 2013-10-08 Cascade Biosystems, Inc. Methods and materials for detecting genetic or epigenetic elements
WO2011146596A1 (en) * 2010-05-18 2011-11-24 Cyvek, Inc. Method and apparatus for performing extraction and enrichment of components in a biological sample
US8512538B2 (en) 2010-05-28 2013-08-20 Integenx Inc. Capillary electrophoresis device
EP2606242A4 (en) 2010-08-20 2016-07-20 Integenx Inc MICROFLUIDIC DEVICES WITH MECHANICALLY SEALED MEMBRANE VALVES
EP2606154B1 (en) 2010-08-20 2019-09-25 Integenx Inc. Integrated analysis system
WO2012122564A2 (en) 2011-03-10 2012-09-13 Xagenic, Inc. Diagnostic and sample preparation devices and methods
AU2012255151B2 (en) 2011-05-18 2015-09-03 Diasorin Italia S.P.A. Systems and methods for detecting the presence of a selected volume of material in a sample processing device
KR101963721B1 (ko) 2011-05-18 2019-03-29 디아소린 에스.피.에이. 샘플 처리 장치 상의 밸빙을 위한 시스템 및 방법
MX336625B (es) 2011-05-18 2016-01-26 3M Innovative Properties Co Sistemas y metodos para medicion volumetrica en dispositivo de procesamiento de muestra.
USD672467S1 (en) 2011-05-18 2012-12-11 3M Innovative Properties Company Rotatable sample processing disk
US20150136604A1 (en) 2011-10-21 2015-05-21 Integenx Inc. Sample preparation, processing and analysis systems
US10865440B2 (en) 2011-10-21 2020-12-15 IntegenX, Inc. Sample preparation, processing and analysis systems
US9855559B2 (en) 2011-12-30 2018-01-02 Abbott Molecular Inc. Microorganism nucleic acid purification from host samples
US10072284B2 (en) 2012-06-21 2018-09-11 Monsanto Technology Llc Lysis buffer and methods for extraction of DNA from plant material
US9399986B2 (en) 2012-07-31 2016-07-26 General Electric Company Devices and systems for isolating biomolecules and associated methods thereof
WO2014031532A1 (en) * 2012-08-19 2014-02-27 University Of Rochester Microfluidic device for filtering fluids and dialysis
US9707555B2 (en) 2012-11-30 2017-07-18 Qvella Corporation Method for pretreatment of microbial samples
US9169521B1 (en) * 2013-03-14 2015-10-27 The Boeing Company Point-of-collection sample preparation device and method
WO2015019195A2 (en) * 2013-08-07 2015-02-12 Xagenic Inc. Systems, devices, and methods for deploying onboard reagents in a diagnostic device
EP3071333A4 (en) 2013-11-18 2017-11-15 IntegenX Inc. Cartridges and instruments for sample analysis
US10596570B2 (en) 2014-05-16 2020-03-24 Qvella Corporation Apparatus, system and method for performing automated centrifugal separation
US10208332B2 (en) 2014-05-21 2019-02-19 Integenx Inc. Fluidic cartridge with valve mechanism
CN107106983B (zh) 2014-10-22 2021-04-16 尹特根埃克斯有限公司 用于样品制备、处理和分析的系统和方法
US20200370036A1 (en) * 2017-08-04 2020-11-26 Microbedx, Inc. Methods for Lysis of Cells Within a Sample
EP3668990A4 (en) * 2017-08-18 2021-03-31 MicrobeDx, Inc. PROCEDURE FOR TESTING ANTIMICROBIAL SUSCEPTIBILITY
US20210245157A1 (en) * 2018-05-14 2021-08-12 Microbedx, Inc. Portable microfludic system for biological and analytical testing
EP3803326A4 (en) 2018-05-25 2022-02-23 Qvella Corporation METHODS AND COMPOSITIONS FOR SELECTIVE LYSIS OF BLOOD CELLS AND SEPARATION OF MICROBIAL CELLS
IT201900008334A1 (it) 2019-06-07 2020-12-07 Nurex Srl Dispositivo automatizzato per l'estrazione, purificazione e concentrazione degli acidi nucleici finalizzato a migliorare la sensibilità e la automazione della rilevazione di cellule nei campioni biologici
CN117255945A (zh) 2021-03-31 2023-12-19 伊鲁米纳公司 纳米孔传感器装置
WO2023081031A1 (en) 2021-11-08 2023-05-11 Illumina, Inc. Identifying nucleotides using changes in impedance between electrodes
CN114672541B (zh) * 2022-03-21 2024-02-02 生工生物工程（上海）股份有限公司 一种核酸释放液、试剂盒及核酸释放的方法
WO2023187112A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Amphiphilic block-co-polymers cross-linked with different cross-linkers consecutively forming a cross-linking hierarchy
US20250223637A1 (en) 2022-03-31 2025-07-10 Illumina, Inc. Devices including osmotically balanced barriers, and methods of making and using the same
US20250205655A1 (en) 2022-03-31 2025-06-26 Illumina, Inc. Amphiphilic polymers to be used in barriers and preparation thereof, barriers with nanopores and preparation thereof
US20230312857A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Nanopore devices including barriers using polymers with end groups, and methods of making the same
WO2023187081A1 (en) 2022-03-31 2023-10-05 Illumina Cambridge Limited Methods for inserting nanopores into polymeric membranes using chaotropic solvents
AU2023246642A1 (en) 2022-03-31 2024-08-29 Illumina, Inc. Nanopore devices including barriers using diblock or triblock copolymers, and methods of making the same
CN119136899A (zh) 2022-03-31 2024-12-13 伊鲁米纳公司 包括交联两亲分子的屏障及其制备方法
US20240392117A1 (en) 2023-05-25 2024-11-28 Illumina, Inc. Methods of making barriers including nanopores and crosslinked amphiphilic molecules, and barriers formed using same
WO2025106652A1 (en) 2023-11-17 2025-05-22 Illumina, Inc. Fluidic devices, nanopore instruments, and methods

Family Cites Families (204)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3157635A (en) 1960-08-05 1964-11-17 Takeda Chemical Industries Ltd Sulfonic acid cation exchange resin purification of nucleotides
US4153661A (en) 1977-08-25 1979-05-08 Minnesota Mining And Manufacturing Company Method of making polytetrafluoroethylene composite sheet
US4399009A (en) 1981-01-19 1983-08-16 Oronzio Denora Impianti Elettrochimici S.P.A. Electrolytic cell and method
US4399235A (en) 1981-05-11 1983-08-16 The Dow Chemical Company High density ion exchange resins
US4565663A (en) 1981-06-26 1986-01-21 Minnesota Mining And Manufacturing Company Method for making water-swellable composite sheet
US4460642A (en) 1981-06-26 1984-07-17 Minnesota Mining And Manufacturing Company Water-swellable composite sheet of microfibers of PTFE and hydrophilic absorptive particles
US4373519A (en) 1981-06-26 1983-02-15 Minnesota Mining And Manufacturing Company Composite wound dressing
US4483920A (en) 1982-05-17 1984-11-20 Hahnemann University Immobilization of message RNA directly from cells onto filter material
US4539256A (en) 1982-09-09 1985-09-03 Minnesota Mining And Manufacturing Co. Microporous sheet material, method of making and articles made therewith
JPS5968344A (ja) 1982-10-12 1984-04-18 Agency Of Ind Science & Technol 非対称機能膜及びその製法
US4780367A (en) 1983-06-27 1988-10-25 Minnesota Mining And Manufacturing Company Tackified star block copolymer pressure-sensitive adhesive composition and the sheet materials coated therewith
US4839296A (en) 1985-10-18 1989-06-13 Chem-Elec, Inc. Blood plasma test method
US4757014A (en) 1985-11-08 1988-07-12 Minnesota Mining And Manufacturing Company Immobilization of biologically active protein on a polymeric fibrous support
US4849311A (en) 1986-09-24 1989-07-18 Toa Nenryo Kogyo Kabushiki Kaisha Immobilized electrolyte membrane
US4726989A (en) 1986-12-11 1988-02-23 Minnesota Mining And Manufacturing Microporous materials incorporating a nucleating agent and methods for making same
US4954444A (en) 1987-03-02 1990-09-04 E. I. Du Pont De Nemours And Company Enzyme immobilization and bioaffinity separations with perfluorocarbon polymer-based supports
US5079155A (en) 1987-03-02 1992-01-07 E. I. Du Pont De Nemours And Company Fluorocarbon polymer support for chromatographic separations, diagnostic assays and enzyme immobilization
US4737560A (en) 1987-03-13 1988-04-12 Minnesota Mining And Manufacturing Company Polymer beads
US4906378A (en) 1987-12-28 1990-03-06 Minnesota Mining And Manufacturing Company Composite chromatographic article
US4971736A (en) 1987-12-28 1990-11-20 Minnesota Mining And Manufacturing Company Method of preparing composite chromatographic article
US4810381A (en) 1987-12-28 1989-03-07 Minnesota Mining And Manufacturing Company Composite chromatographic article
US4923978A (en) 1987-12-28 1990-05-08 E. I. Du Pont De Nemours & Company Process for purifying nucleic acids
US5205929A (en) 1988-02-03 1993-04-27 Regents Of The University Of Minnesota High stability porous zirconium oxide spherules
US5015373A (en) 1988-02-03 1991-05-14 Regents Of The University Of Minnesota High stability porous zirconium oxide spherules
US5141634A (en) 1988-02-03 1992-08-25 Regents Of The University Of Minnesota High stability porous zirconium oxide spherules
US5011861A (en) 1988-06-28 1991-04-30 Millipore Corporation Membranes for solid phase protein sequencing
US5182377A (en) 1988-09-09 1993-01-26 Hoffmann-La Roche Inc. Probes for detection of human papillomavirus
DE3921498A1 (de) 1988-09-28 1990-03-29 Bayer Ag Polymer-gebundene-farbstoffe, verfahren zu deren herstellung und verwendung
US4957943A (en) 1988-10-14 1990-09-18 Minnesota Mining And Manufacturing Company Particle-filled microporous materials
US5512439A (en) 1988-11-21 1996-04-30 Dynal As Oligonucleotide-linked magnetic particles and uses thereof
AU5188190A (en) 1989-02-06 1990-08-24 Gene-Trak Systems Probes and methods for the detection of listeria
US5182083A (en) 1989-03-13 1993-01-26 Beckman Instruments, Inc. Sample wheel for chemistry analyzers
US5234809A (en) 1989-03-23 1993-08-10 Akzo N.V. Process for isolating nucleic acid
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
US5010183A (en) 1989-07-07 1991-04-23 Macfarlane Donald E Process for purifying DNA and RNA using cationic detergents
EP0409432A3 (en) 1989-07-20 1991-12-11 Warner-Lambert Company Confectionery delivery system
US5231015A (en) 1989-10-18 1993-07-27 Eastman Kodak Company Methods of extracting nucleic acids and pcr amplification without using a proteolytic enzyme
JP2978187B2 (ja) 1989-11-02 1999-11-15 日本ケミカルリサーチ株式会社 修飾スーパーオキサイドディスムターゼの製造法
US5187066A (en) 1990-02-14 1993-02-16 Syntex (U.S.A.) Inc. Methods for detecting amphiphilic antigens
US5207915A (en) 1990-02-23 1993-05-04 Minnesota Mining And Manufacturing Company Separation method using controlled pore composite polytetrafluoroethylene article
US5147539A (en) 1990-02-23 1992-09-15 Minnesota Mining And Manufacturing Company Controlled pore composite polytetrafluoroethylene article
US5071610A (en) 1990-02-23 1991-12-10 Minnesota Mining And Manufacturing Company Method of making a controlled pore composite polytetrafluoroethylene
US5770029A (en) 1996-07-30 1998-06-23 Soane Biosciences Integrated electrophoretic microdevices
EP0447362A1 (en) 1990-03-13 1991-09-18 Warner-Lambert Company Improved ingestible anion exchange resin delivery system compositions containing adipic acid
US5271833A (en) 1990-03-22 1993-12-21 Regents Of The University Of Minnesota Polymer-coated carbon-clad inorganic oxide particles
US5182016A (en) 1990-03-22 1993-01-26 Regents Of The University Of Minnesota Polymer-coated carbon-clad inorganic oxide particles
US5108597A (en) 1990-03-22 1992-04-28 Regents Of The University Of Minnesota Carbon-clad zirconium oxide particles
US5254262A (en) 1990-03-22 1993-10-19 Regents Of The University Of Minnesota Carbon-clad zirconium oxide particles
US5019232A (en) 1990-06-01 1991-05-28 Minnesota Mining And Manufacturing Company Medium for electrophoresis
US5334316A (en) 1990-10-10 1994-08-02 Brigham Young University Process of using polytetraalkylammonium and polytrialkylamine-containing ligands bonded to inorganic supports for removing and concentrating desired ions from solutions
US5200471A (en) 1990-11-05 1993-04-06 Minnesota Mining And Manufacturing Company Biomolecules covalently immobilized with a high bound specific biological activity and method of preparing same
US5187083A (en) 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
US5284940A (en) 1990-11-14 1994-02-08 Hri Research, Inc. Preparation for nucleic acid samples
US5620852A (en) 1990-11-14 1997-04-15 Hri Research, Inc. Nucleic acid preparation methods
US5294668A (en) 1990-11-15 1994-03-15 Minnesota Mining And Manufacturing Company Polyolefin pressure-sensitive adhesive compositions containing macromonomers
CA2059398C (en) 1991-02-07 1999-05-25 Craig G. Markell Solid phase extraction medium
USRE36811E (en) 1991-02-07 2000-08-08 Minnesota Mining And Manufacturing Co. Solid phase extraction medium
US5264184A (en) 1991-03-19 1993-11-23 Minnesota Mining And Manufacturing Company Device and a method for separating liquid samples
ES2081613T3 (es) 1991-03-21 1996-03-16 Johnson & Johnson Clin Diag Elemento y procedimiento para amplificacion y deteccion de acido nucleico usando muestras adheridas.
WO1992018514A1 (en) 1991-04-12 1992-10-29 Minnesota Mining And Manufacturing Company Purification of nucleic acids using metal oxide supports
CA2067711C (en) 1991-05-03 2000-08-08 Daniel Lee Woodard Solid phase extraction purification of dna
US5238621A (en) 1991-06-28 1993-08-24 Minnesota Mining And Manufacturing Company Method of controlling porosity in a composite article
JP3087246B2 (ja) 1991-07-11 2000-09-11 インターナショナル フラワー ディベロップメンツ プロプライアタリー リミティド フラボノイド経路の酵素をコードする遺伝子配列及びその使用
FR2679255B1 (fr) 1991-07-17 1993-10-22 Bio Merieux Procede d'immobilisation d'un fragment nucleique par fixation passive sur un support solide, support solide ainsi obtenu et son utilisation.
US6093558A (en) 1991-07-25 2000-07-25 Edge Biosystems, Inc. Binding protein of biologically active compositions to an adhesive formulation on a substrate
US5993935A (en) 1991-10-11 1999-11-30 3M Innovative Properties Company Covalently reactive particles incorporated in a continous porous matrix
US5328758A (en) 1991-10-11 1994-07-12 Minnesota Mining And Manufacturing Company Particle-loaded nonwoven fibrous article for separations and purifications
US5380901A (en) 1992-01-30 1995-01-10 The United States Of America As Represented By The Secretary Of Commerce Multifunctional acrylates and the synthesis thereof
US5438128A (en) 1992-02-07 1995-08-01 Millipore Corporation Method for rapid purifiction of nucleic acids using layered ion-exchange membranes
US5183705A (en) 1992-02-28 1993-02-02 Minnesota Mining And Manufacturing Company Pressure-sensitive adhesive composition having high shear strength
CA2096193A1 (en) 1992-06-01 1993-12-02 Daniel L. Woodard Chemically synthesized silanes that bind nucleic acids
AU687352B2 (en) * 1992-06-08 1998-02-26 Gen-Probe Incorporated Preparation of nucleic acid from mononuclear cells
US5344701A (en) 1992-06-09 1994-09-06 Minnesota Mining And Manufacturing Company Porous supports having azlactone-functional surfaces
EP0647232B1 (en) 1992-06-23 1999-04-28 Minnesota Mining And Manufacturing Company Deproteinization with azlactone-functional supports
ATE161866T1 (de) 1992-10-21 1998-01-15 Cornell Res Foundation Inc Selektive, die porengrösse betreffende chemische modifikation poröser materialien
AU669398B2 (en) 1992-11-13 1996-06-06 Becton Dickinson & Company Boron silicates aluminum silicates phosphosilicates and purification of DNA
AT398973B (de) 1992-11-18 1995-02-27 Bonn Guenther Dr Verfahren zur trennung von nukleinsäuren
US5637687A (en) 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
US5408002A (en) 1993-09-09 1995-04-18 Minnesota Mining And Manufacturing Company Azlactone-functional polymer blends, articles produced therefrom and methods for preparing both
US5438127A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using a PCl3 modified glass fiber membrane
US5438129A (en) 1993-09-27 1995-08-01 Becton Dickinson And Company DNA purification by solid phase extraction using partially fluorinated aluminum hydroxide adsorbant
JP3866762B2 (ja) 1993-11-29 2007-01-10 ジェン−プローブ・インコーポレイテッド 広範な生物からの核酸抽出法
US5610287A (en) 1993-12-06 1997-03-11 Molecular Tool, Inc. Method for immobilizing nucleic acid molecules
DE69412286T2 (de) 1993-12-22 1998-12-03 Minnesota Mining And Mfg. Co., Saint Paul, Minn. Verwendung von blattförmigen materialien für festphasenextraktionen und festphasenreaktionen
US6207251B1 (en) 1994-01-10 2001-03-27 Minnesota Mining And Manufacturing Company Reinforced particle-loaded fibrillated PTFE web
US6780818B2 (en) 1994-02-02 2004-08-24 The Regents Of The University Of California Quantitative organic vapor-particle sampler
WO1995024505A1 (en) 1994-03-08 1995-09-14 Amersham International Plc Modifying nucleotide analogues
US5529686A (en) 1994-07-15 1996-06-25 Minnesota Mining And Manufacturing Company Composite membranes for solid phase extractions and reactions
US5625053A (en) 1994-08-26 1997-04-29 Board Of Regents For Northern Illinois Univ. Method of isolating purified plasmid DNA using a nonionic detergent, solution
SE9500183D0 (sv) 1995-01-20 1995-01-20 Pharmacia Biotech Ab Method for the purifivation of short nucleic acids
US5472600A (en) 1995-02-01 1995-12-05 Minnesota Mining And Manufacturing Company Gradient density filter
US6063838A (en) 1995-02-16 2000-05-16 3M Innovative Properties Company Blended pressure-sensitive adhesives
US5705059A (en) 1995-02-27 1998-01-06 Miltenyi; Stefan Magnetic separation apparatus
US5741828A (en) 1995-05-01 1998-04-21 S.K.Y. Polymers, Inc. Flexible hydrophilic composite coatings
US5709943A (en) 1995-05-04 1998-01-20 Minnesota Mining And Manufacturing Company Biological adsorption supports
US6143247A (en) 1996-12-20 2000-11-07 Gamera Bioscience Inc. Affinity binding-based system for detecting particulates in a fluid
US6168948B1 (en) * 1995-06-29 2001-01-02 Affymetrix, Inc. Miniaturized genetic analysis systems and methods
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US5700902A (en) 1995-07-27 1997-12-23 Circe Biomedical, Inc. Block copolymers
DE19530132C2 (de) 1995-08-16 1998-07-16 Max Planck Gesellschaft Verfahren zur Reinigung, Stabilisierung oder Isolierung von Nukleinsäuren aus biologischen Materialien
US5804684A (en) 1995-08-24 1998-09-08 The Theobald Smith Research Institute, Inc. Method for isolating nucleic acids
US5620869A (en) 1995-09-28 1997-04-15 Becton, Dickinson And Company Methods for reducing inhibition of nucleic acid amplification reactions
US20010055812A1 (en) 1995-12-05 2001-12-27 Alec Mian Devices and method for using centripetal acceleration to drive fluid movement in a microfluidics system with on-board informatics
US6068751A (en) 1995-12-18 2000-05-30 Neukermans; Armand P. Microfluidic valve and integrated microfluidic system
JP3818689B2 (ja) 1996-01-16 2006-09-06 富士写真フイルム株式会社 コロイド状シリカをコアとし、有機ポリマーをシェルとするコア／シェル状複合粒子の水性分散物及びその製造方法
WO1997027325A2 (en) 1996-01-23 1997-07-31 Rapigene, Inc. Methods and compositions for analyzing nucleic acid molecules utilizing sizing techniques
FR2744803B1 (fr) 1996-02-12 1998-03-13 Bio Merieux Procede et dispositif de traitement d'une carte d'analyse
US5904848A (en) 1996-02-21 1999-05-18 Cpg, Inc. Controlled pore glass-synthetic resin membrane
US5837203A (en) 1996-04-09 1998-11-17 Sievers Instruments, Inc. Device to alternately supply a fluid to an analyzer
US5882521A (en) 1996-04-18 1999-03-16 Waters Investment Ltd. Water-wettable chromatographic media for solid phase extraction
US6074827A (en) 1996-07-30 2000-06-13 Aclara Biosciences, Inc. Microfluidic method for nucleic acid purification and processing
US6143248A (en) 1996-08-12 2000-11-07 Gamera Bioscience Corp. Capillary microvalve
DE19731670C2 (de) 1997-07-23 2000-06-29 Dorothea Waschk Verfahren zur Reinigung und gegebenenfalls Analyse von Nukleinsäuren aus biologischen Proben
AU4775497A (en) 1996-09-19 1998-04-14 W. Kurt Roth Method for purifying and eventually analyzing nucleic acids from biological test samples
US6048550A (en) 1996-10-03 2000-04-11 Chan; Daniel C. F. Hydrophilic microparticles and methods to prepare same
US6093559A (en) 1996-10-24 2000-07-25 Corning Incorporated Producing low binding hydrophobic surfaces by treating with a low HLB number non-ionic surfactant
US5786219A (en) * 1996-10-28 1998-07-28 Molecular Probes, Inc. Microspheres with fluorescent spherical zones
CA2271846A1 (en) 1996-11-12 1998-05-22 Dean T. Tsou Hydrophilic polymeric phase inversion membrane
SE9700383D0 (sv) 1997-02-04 1997-02-04 Pharmacia Biotech Ab An adsorption/separation method and a medium for adsorption/separation
US6048457A (en) 1997-02-26 2000-04-11 Millipore Corporation Cast membrane structures for sample preparation
US5997818A (en) 1997-02-27 1999-12-07 Minnesota Mining And Manufacturing Company Cassette for tonometric calibration
SE9700769D0 (sv) 1997-03-04 1997-03-04 Pharmacia Biotech Ab Matriser för separation och separation som utnyttjar matriserna
US5999935A (en) 1997-03-28 1999-12-07 International Business Machines Corporation Tail compression of a sparse log stream of a multisystem environment
US6632399B1 (en) 1998-05-22 2003-10-14 Tecan Trading Ag Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system for performing biological fluid assays
EP0983504A2 (en) * 1997-05-23 2000-03-08 Gamera Bioscience Corporation Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
US5919626A (en) 1997-06-06 1999-07-06 Orchid Bio Computer, Inc. Attachment of unmodified nucleic acids to silanized solid phase surfaces
US6348336B1 (en) * 1997-07-01 2002-02-19 Alpha Therapeutic Corporation Process for purification of PCR test samples
EP0897978A3 (en) 1997-08-22 2001-10-17 Becton, Dickinson and Company Zirconium oxide and related compounds for purification of nucleic acids
US6451260B1 (en) 1997-08-26 2002-09-17 Dyax Corp. Method for producing microporous elements, the microporous elements thus produced and uses thereof
EP1019696A4 (en) 1997-09-19 2003-07-23 Aclara Biosciences Inc APPARATUS AND METHOD FOR TRANSFERING LIQUIDS
EP1019712A4 (en) 1997-09-19 2002-08-07 Aclara Biosciences Inc APPARATUS AND METHOD FOR HAIR ELECTROFLUIDITY
DE19746874A1 (de) 1997-10-23 1999-04-29 Qiagen Gmbh Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
FI974124A0 (fi) 1997-11-04 1997-11-04 Satu Aokerman Foerfarande foer separering av proteinfria aemnen fraon proteinhaltiga aemnen foer daerpao foeljande behandling
US6241980B1 (en) 1997-11-04 2001-06-05 Becton, Dickinson And Company Sample processing method using ion exchange resin
US6475722B1 (en) 1997-12-03 2002-11-05 Curagen Corporation Surface treatments for DNA processing devices
DE69839709D1 (de) 1997-12-24 2008-08-21 Cepheid Vorrichtung und Verfahren zur Lyse
AU2347799A (en) 1998-01-29 1999-08-16 University Of Pittsburgh Thermal expansion-induced fluid control for microfluidic devices
EP1062510A2 (en) 1998-03-10 2000-12-27 Strategic Diagnostics Inc. Integrated assay device and methods of production and use
US6265168B1 (en) 1998-10-06 2001-07-24 Transgenomic, Inc. Apparatus and method for separating and purifying polynucleotides
US6534262B1 (en) 1998-05-14 2003-03-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
US6074927A (en) 1998-06-01 2000-06-13 Advanced Micro Devices, Inc. Shallow trench isolation formation with trench wall spacer
WO2000000816A1 (de) 1998-06-29 2000-01-06 Evotec Biosystems Ag Verfahren und vorrichtung zur manipulation von partikeln in mikrosystemen
US6103199A (en) 1998-09-15 2000-08-15 Aclara Biosciences, Inc. Capillary electroflow apparatus and method
US6572830B1 (en) * 1998-10-09 2003-06-03 Motorola, Inc. Integrated multilayered microfludic devices and methods for making the same
US6277488B1 (en) 1998-10-28 2001-08-21 3M Innovative Properties Company Adhesive composition containing a block copolymer composition and polyphenylene oxide resin and products thereof
US6240790B1 (en) 1998-11-09 2001-06-05 Agilent Technologies, Inc. Device for high throughout sample processing, analysis and collection, and methods of use thereof
WO2000045180A1 (en) 1999-02-01 2000-08-03 3M Innovative Properties Company Poly(alpha-olefin) adhesive cover tapes for analytical receptacles
WO2000050887A1 (de) 1999-02-22 2000-08-31 Evotec Biosystems Ag Verwendung von trägermaterial in der kapillar-elektrochromatographie
US6479300B1 (en) 1999-03-15 2002-11-12 Millipore Corporation Metal loaded ligand bound membranes for metal ion affinity chromatography
US6306273B1 (en) 1999-04-13 2001-10-23 Aclara Biosciences, Inc. Methods and compositions for conducting processes in microfluidic devices
JP2002544320A (ja) 1999-05-05 2002-12-24 スリーエム イノベイティブ プロパティズ カンパニー シリコーン接着剤、製品、および方法
DE60012562D1 (de) 1999-06-18 2004-09-02 Gamera Bioscience Corp Vorrichtungen und verfahren zur durchführung miniaturisierter homogener tests
US6664104B2 (en) 1999-06-25 2003-12-16 Cepheid Device incorporating a microfluidic chip for separating analyte from a sample
GB9915398D0 (en) 1999-07-02 1999-09-01 Baker Matthew J Magnetic particles
US6383783B1 (en) 1999-09-21 2002-05-07 3M Innovative Properties Company Nucleic acid isolation by adhering to hydrophobic solid phase and removing with nonionic surfactant
US6414136B1 (en) 1999-10-06 2002-07-02 Prolinx, Inc. Removal of dye-labeled dideoxy terminators from DNA sequencing reactions
JP4855616B2 (ja) 1999-10-27 2012-01-18 スリーエム イノベイティブ プロパティズ カンパニー フルオロケミカルスルホンアミド界面活性剤
DK1232502T3 (da) 1999-11-17 2006-05-22 Roche Diagnostics Gmbh Magnetiske glaspartikler, fremgangsmåde til deres fremstilling samt anvendelser deraf
GB9927904D0 (en) 1999-11-25 2000-01-26 Amersham Pharm Biotech Ab A method fro obtaining a nucleic acid variant
CA2290731A1 (en) 1999-11-26 2001-05-26 D. Jed Harrison Apparatus and method for trapping bead based reagents within microfluidic analysis system
US7311880B2 (en) 1999-12-23 2007-12-25 3M Innovative Properties Company Well-less filtration device
US6692596B2 (en) 1999-12-23 2004-02-17 3M Innovative Properties Company Micro-titer plate and method of making same
US6875348B2 (en) 2000-02-18 2005-04-05 The Board Of Trustees Of The Leland Stanford Junior University Separation column having a photopolymerized sol-gel component and associated methods
AU3986501A (en) 2000-02-23 2001-09-03 Zyomyx Inc Chips having elevated sample surfaces
AU2001238616A1 (en) 2000-02-23 2001-09-03 Wen Shao Rapid nucleic acid separation, isolation and purification methods
EP1134586A1 (en) 2000-03-08 2001-09-19 Tibotec N.V. Method for adding a fluid in a series of wells
AU4099001A (en) 2000-03-13 2001-09-24 Genset Nucleic acid detection method and system
JP2003526796A (ja) 2000-03-14 2003-09-09 ハメン・コーポレイション 間入ポリマーネットワークを有する複合マトリックス
WO2001071732A2 (en) 2000-03-24 2001-09-27 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
JP2003533681A (ja) 2000-05-15 2003-11-11 テカン・トレーディング・アクチェンゲゼルシャフト ミクロ流体装置および細胞ベースの分析を実行する方法
US6627159B1 (en) 2000-06-28 2003-09-30 3M Innovative Properties Company Centrifugal filling of sample processing devices
US6720187B2 (en) 2000-06-28 2004-04-13 3M Innovative Properties Company Multi-format sample processing devices
US6734401B2 (en) 2000-06-28 2004-05-11 3M Innovative Properties Company Enhanced sample processing devices, systems and methods
US6504021B2 (en) 2000-07-05 2003-01-07 Edge Biosystems, Inc. Ion exchange method for DNA purification
US6537502B1 (en) 2000-07-25 2003-03-25 Harvard Apparatus, Inc. Surface coated housing for sample preparation
US6503716B1 (en) 2000-11-28 2003-01-07 Pe Corporation (Ny) Compositions and methods for extracting a nucleic acid
US20030017567A1 (en) 2001-04-24 2003-01-23 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US6617136B2 (en) 2001-04-24 2003-09-09 3M Innovative Properties Company Biological sample processing methods and compositions that include surfactants
US7374724B2 (en) 2001-05-29 2008-05-20 Tecan Trading Ag Device for processing samples, use of the device, and method for producing the device
US7138436B2 (en) 2001-06-13 2006-11-21 3M Innovative Properties Company Uncrosslinked foams made from emulsions
US6919058B2 (en) 2001-08-28 2005-07-19 Gyros Ab Retaining microfluidic microcavity and other microfluidic structures
ATE477054T1 (de) 2001-09-17 2010-08-15 Gyros Patent Ab Einen kontrollierten strom in einer mikrofluidvorrichtung ermöglichende funktionseinheit
US7192560B2 (en) 2001-12-20 2007-03-20 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using anion exchange
US7347976B2 (en) 2001-12-20 2008-03-25 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using a hydrophilic solid support in a hydrophobic matrix
US6532997B1 (en) 2001-12-28 2003-03-18 3M Innovative Properties Company Sample processing device with integral electrophoresis channels
US7662340B2 (en) 2001-12-28 2010-02-16 Hitachi High-Technologies Corporation Extractor chemical analyzer and chemical analyzing method
US6833238B2 (en) 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
US6723236B2 (en) 2002-03-19 2004-04-20 Waters Investments Limited Device for solid phase extraction and method for purifying samples prior to analysis
JP2006505766A (ja) 2002-04-11 2006-02-16 バースタイン テクノロジーズ，インコーポレイティド 分析ディスクを含むマルチパラメータ検定とその関連方法
US6998271B2 (en) 2002-06-03 2006-02-14 The Hong Kong Polytechnic University Luminescent sensory material for organic-halogen compounds, and methods and apparatus utilizing such
US20040023220A1 (en) 2002-07-23 2004-02-05 Lawrence Greenfield Integrated method for PCR cleanup and oligonucleotide removal
JP2005533652A (ja) 2002-07-26 2005-11-10 アプレラ コーポレイション 微小流体サイズ排除デバイス、システム、および方法
US20040016702A1 (en) 2002-07-26 2004-01-29 Applera Corporation Device and method for purification of nucleic acids
US7214348B2 (en) 2002-07-26 2007-05-08 Applera Corporation Microfluidic size-exclusion devices, systems, and methods
AU2003256787A1 (en) 2002-07-26 2004-02-16 Applera Corporation Petal-array support for use with microplates
US20040055956A1 (en) 2002-07-26 2004-03-25 Harrold Michael P. Microfluidic device including purification column with excess diluent, and method
US7332348B2 (en) 2003-02-28 2008-02-19 Applera Corporation Sample substrate having a divided sample chamber and method of loading thereof
US7981600B2 (en) 2003-04-17 2011-07-19 3M Innovative Properties Company Methods and devices for removal of organic molecules from biological mixtures using an anion exchange material that includes a polyoxyalkylene
US6883238B1 (en) * 2003-09-08 2005-04-26 Khiem Tran Hairstyling scissors
US20050130177A1 (en) 2003-12-12 2005-06-16 3M Innovative Properties Company Variable valve apparatus and methods
US7322254B2 (en) 2003-12-12 2008-01-29 3M Innovative Properties Company Variable valve apparatus and methods
US20050142571A1 (en) 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using solid phase material
US20050142570A1 (en) 2003-12-24 2005-06-30 3M Innovative Properties Company Methods for nucleic acid isolation and kits using a microfluidic device and sedimenting reagent
US7727710B2 (en) 2003-12-24 2010-06-01 3M Innovative Properties Company Materials, methods, and kits for reducing nonspecific binding of molecules to a surface

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