AU2004288134B2 - Improved glucose dehydrogenase polypeptides and related polynucleotides - Google Patents

Improved glucose dehydrogenase polypeptides and related polynucleotides Download PDF

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Publication number
AU2004288134B2
AU2004288134B2 AU2004288134A AU2004288134A AU2004288134B2 AU 2004288134 B2 AU2004288134 B2 AU 2004288134B2 AU 2004288134 A AU2004288134 A AU 2004288134A AU 2004288134 A AU2004288134 A AU 2004288134A AU 2004288134 B2 AU2004288134 B2 AU 2004288134B2
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Australia
Prior art keywords
polypeptide
seq
gdh
naturally occurring
acid sequence
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AU2004288134A
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AU2004288134A1 (en
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S. Christopher Davis
Stephane J. Jenne
Anke Krebber
Lisa Marie Newman
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Codexis Inc
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Codexis Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2004288134A 2003-08-11 2004-08-11 Improved glucose dehydrogenase polypeptides and related polynucleotides Expired AU2004288134B2 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US49430003P 2003-08-11 2003-08-11
US60/494,300 2003-08-11
US54565704P 2004-02-18 2004-02-18
US60/545,657 2004-02-18
PCT/US2004/026194 WO2005045016A2 (en) 2003-08-11 2004-08-11 Improved glucose dehydrogenase polypeptides and related polynucleotides

Publications (2)

Publication Number Publication Date
AU2004288134A1 AU2004288134A1 (en) 2005-05-19
AU2004288134B2 true AU2004288134B2 (en) 2009-12-17

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Family Applications (1)

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AU2004288134A Expired AU2004288134B2 (en) 2003-08-11 2004-08-11 Improved glucose dehydrogenase polypeptides and related polynucleotides

Country Status (11)

Country Link
US (2) US7816111B2 (enExample)
EP (1) EP1660648B1 (enExample)
JP (1) JP2007502114A (enExample)
KR (1) KR20060064620A (enExample)
AU (1) AU2004288134B2 (enExample)
BR (1) BRPI0413492A (enExample)
CA (1) CA2535147A1 (enExample)
IL (1) IL173260A0 (enExample)
MX (1) MXPA06001719A (enExample)
NO (1) NO20060543L (enExample)
WO (1) WO2005045016A2 (enExample)

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003258187A1 (en) 2002-08-09 2004-02-25 Codexis, Inc. Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives
EP1584675B1 (en) 2002-12-24 2010-02-24 Ikeda Food Research Co. Ltd. Coenzyme-binding glucose dehydrogenase
GB0330016D0 (en) * 2003-12-24 2004-01-28 Danisco Method
MXPA06001719A (es) 2003-08-11 2006-05-19 Codexis Inc Polipeptidos de glucosa deshidrogenasa mejorados, y polinucleotidos relacionados.
WO2005018579A2 (en) 2003-08-11 2005-03-03 Codexis, Inc. Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives and vicinal cyano, hydroxy substituted carboxylic acid esters
CA2550800A1 (en) * 2003-12-24 2005-07-21 Danisco A/S Methods to produce variant glycolipid acyltransferases and variant glycolipid acyltransferases with enhanced activity towards glycolipids.
DE102004059376A1 (de) * 2004-12-09 2006-06-22 Consortium für elektrochemische Industrie GmbH GDH-Mutante mit verbesserter chemischer Stabilität
WO2006101239A1 (ja) 2005-03-25 2006-09-28 Ikeda Food Research Co., Ltd. 補酵素結合型グルコース脱水素酵素及びこれをコードするポリヌクレオチド
DE102005036880A1 (de) * 2005-08-02 2007-02-08 Julich Chiral Solutions Gmbh Stereoselektive Synthese von chiralen Diolen
WO2008042876A2 (en) 2006-10-02 2008-04-10 Codexis, Inc. Compositions and methods for producing stereoisomerically pure statins and synthetic intermediates therefor
JP4348563B2 (ja) * 2006-11-14 2009-10-21 東洋紡績株式会社 改変型フラビンアデニンジヌクレオチド依存性グルコースデヒドロゲナーゼ
JP2009034063A (ja) * 2007-08-03 2009-02-19 Kao Corp アルカリプロテアーゼの安定性向上方法
JP2009034062A (ja) * 2007-08-03 2009-02-19 Kao Corp アルカリプロテアーゼ
JP2010536385A (ja) 2007-08-24 2010-12-02 コデクシス， インコーポレイテッド （ｒ）−３−ヒドロキシチオランの立体選択的生成のための改善されたケトレダクターゼポリペプチド
EP2093284A1 (de) 2008-02-19 2009-08-26 F.Hoffmann-La Roche Ag Stabilisierung von Dehydrogenasen mit stabilen Coenzymen
WO2010025238A2 (en) 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of a 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010025287A2 (en) * 2008-08-27 2010-03-04 Codexis, Inc. Ketoreductase polypeptides for the production of 3-aryl-3-hydroxypropanamine from a 3-aryl-3-ketopropanamine
WO2010027710A2 (en) 2008-08-27 2010-03-11 Codexis, Inc. Ketoreductase polypeptides and uses thereof
SI2329014T1 (sl) 2008-08-29 2015-01-30 Codexis, Inc. Polipeptidi ketoreduktaze za stereoselektivno produkcijo (4s)-3(5s)-5(4-fluorofenil)-5-hidroksipentanoil)-4-fenil-1,3-oksazolidin -2-ona
EP2398909B1 (de) 2009-02-19 2015-07-22 F. Hoffmann-La Roche AG Schnelle reaktionskinetik von enzymen mit niedriger aktivität in trockenen chemieschichten
CA2750474C (en) 2009-02-19 2017-07-25 F. Hoffmann-La Roche Ag Fast reaction kinetics of enzymes having low activity in dry chemistry layers
HUE055413T2 (hu) 2009-06-22 2021-11-29 Codexis Inc Ketoreduktáz-közvetített sztereoszelektív útvonal alfa-klóralkoholokhoz
WO2011022548A2 (en) 2009-08-19 2011-02-24 Codexis, Inc. Ketoreductase polypeptides for the preparation of phenylephrine
EP2292751A1 (de) 2009-08-20 2011-03-09 Roche Diagnostics GmbH Stabilisierung von Enzymen mit stabilen Coenzymen
WO2011068050A1 (ja) * 2009-12-05 2011-06-09 天野エンザイム株式会社 変異酵素及びその用途
WO2011071982A2 (en) 2009-12-08 2011-06-16 Codexis, Inc. Synthesis of prazole compounds
EP2513648B1 (de) 2009-12-16 2015-05-27 F.Hoffmann-La Roche Ag Detektion der zersetzung von enzymen in einem testelement durch kontrollierte freisetzung von geschütztem analyt
HUE026367T2 (en) 2010-05-04 2016-06-28 Codexis Inc Biocatalysts for ezetimibe synthesis
DK2649187T3 (da) 2010-12-08 2018-01-29 Codexis Inc Biokatalysatorer og fremgangsmåder til syntesen af armodafinil
WO2012133761A1 (ja) * 2011-03-30 2012-10-04 ユニチカ株式会社 改変型グルコースデヒドロゲナーゼ
HUE038800T2 (hu) 2011-04-13 2018-11-28 Codexis Inc Biokatalitikus eljárás eszlikarbazepin és ennek analógjai elõállítására
CN106479990B (zh) 2011-11-18 2020-09-18 科德克希思公司 用于制备羟基取代的氨基甲酸酯的生物催化剂
EP2636750A1 (en) 2012-03-06 2013-09-11 Roche Diagniostics GmbH Compatible solute ectoine as well as derivatives thereof for enzyme stabilization
WO2013170050A1 (en) 2012-05-11 2013-11-14 Codexis, Inc. Engineered imine reductases and methods for the reductive amination of ketone and amine compounds
EP2770064A1 (de) 2013-02-22 2014-08-27 F. Hoffmann-La Roche AG Hocheffiziente Herstellung von Blutglucose-Teststreifen
US10150959B2 (en) 2013-03-15 2018-12-11 Merck Sharp & Dohme Corp. Immobilized ketoreductases and process for making and using immobilized ketoreductase
WO2014170917A1 (en) 2013-04-17 2014-10-23 Council Of Scientific & Industrial Research Designer cells for enantioselective reduction of ketones and use thereof in efficient production of enantioenriched alcohols
EP2994536B1 (en) 2013-05-08 2017-06-14 Roche Diabetes Care GmbH Stabilization of enzymes by nicotinic acid
CN106232619B (zh) 2013-11-13 2022-09-09 科德克希思公司 工程化的亚胺还原酶和用于酮和胺化合物的还原胺化的方法
WO2015078899A1 (en) 2013-11-27 2015-06-04 Roche Diagnostics Gmbh Composition comprising up-converting phosphors for detecting an analyte
EP2927319A1 (en) 2014-03-31 2015-10-07 Roche Diagnostics GmbH High load enzyme immobilization by crosslinking
CN106164054B (zh) 2014-04-14 2019-05-07 豪夫迈·罗氏有限公司 吩嗪介导剂
EP3183246B1 (en) 2014-08-22 2020-09-23 Roche Diagnostics GmbH Redoxindicators
CN107002050A (zh) 2014-11-25 2017-08-01 科德克希思公司 工程化亚胺还原酶和用于酮和胺化合物的还原胺化的方法
DK3256577T3 (da) 2015-02-10 2020-09-14 Codexis Inc Ketoreduktase polypeptider til syntesen af chirale forbindelser
JP2019529935A (ja) 2016-10-05 2019-10-17 エフ ホフマン−ラ ロッシュ アクチェン ゲゼルシャフト 多検体診断用試験エレメントのための検出試薬および電極配置、ならびにそれらを使用する方法
EP3339431A1 (en) * 2016-12-22 2018-06-27 Roche Diabetes Care GmbH Glucose dehydrogenase variants with improved properties
AU2018383752B2 (en) 2017-12-13 2025-01-30 Codexis, Inc. Carboxyesterase polypeptides for amide coupling
ES2975412T3 (es) 2018-06-21 2024-07-05 Fis Fabbrica Italiana Sintetici Spa Proceso enzimático para la preparación de droxidopa
JP6480629B1 (ja) * 2018-08-07 2019-03-13 キッコーマン株式会社 グルコースデヒドロゲナーゼの組換え製造方法
US12618050B2 (en) 2018-12-10 2026-05-05 The Regents Of The University Of California Engineered polypeptides that exhibit increased catalytic efficiency for unnatural cofactors and uses thereof
US12215359B2 (en) 2019-05-01 2025-02-04 Codexis, Inc. Engineered glucose dehydrogenases and methods for the reductive amination of ketone and amine compounds
IL322082A (en) 2023-01-12 2025-09-01 Novartis Ag Engineered ketoreductase polypeptides

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004015132A2 (en) * 2002-08-09 2004-02-19 Codexis, Inc. Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives
US7132267B2 (en) * 2002-08-09 2006-11-07 Codexis, Inc. Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives and vicinal cyano, hydroxy substituted carboxylic acid esters

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3019450A1 (de) 1980-05-21 1981-11-26 Boehringer Mannheim Gmbh, 6800 Mannheim Verfahren zur gewinnung von glucosedehydrogenase und hierfuer geeigneter mikroorganismus
US4542098A (en) 1983-05-06 1985-09-17 Institut Francais Du Petrole Production of glucose dehydrogenase and use of the resultant enzyme in the enzymatic synthesis of L-carnitine
US4877733A (en) 1986-05-02 1989-10-31 Toyo Jozo Kabushiki Kaisha Glucose dehydrogenase and its production
DE3711881A1 (de) 1987-04-08 1988-10-27 Merck Patent Gmbh Verfahren zur herstellung von glucosedehydrogenase aus bacillus megaterium
DE3808531C1 (enExample) 1988-03-15 1989-07-13 Eppendorf - Netheler - Hinz Gmbh, 2000 Hamburg, De
JPH0286779A (ja) 1988-09-22 1990-03-27 Amano Pharmaceut Co Ltd 改良型組換えｄｎａ、それを含む形質転換体及びそれを用いた耐熱性グルコースデヒドロゲナーゼの製造法
JP2850515B2 (ja) 1990-09-25 1999-01-27 東洋紡績株式会社 グルコースデヒドロゲナーゼおよびその製造法
GB9512837D0 (en) 1995-06-23 1995-08-23 Zeneca Ltd reduction of ketone groups
AU755543B2 (en) 1997-12-19 2002-12-12 Warner-Lambert Export Limited Process for the synthesis of 1,3-diols
WO1999057109A1 (en) 1998-04-30 1999-11-11 Kaneka Corporation Process for producing 6-cyanomethyl-1,3-dioxane-4-acetic acid derivatives
HUP0101122A3 (en) 1998-08-05 2006-02-28 Kaneka Corp Process for the preparation of optically active 2-[6-(hydroxymethyl)-1,3-dioxan-4-yl]acetic acid derivatives
BR0008370A (pt) * 1999-02-19 2001-11-06 Merck Patent Gmbh Proteìnas de fusão de glicose deshidrogenase e uso das mesmas em sistemas de expressão
JP2003504070A (ja) 1999-07-09 2003-02-04 フォルシュングスツェントルム ユーリッヒ ゲゼルシャフト ミット ベシュレンクテル ハフツング ケトカルボン酸及びそのエステルの還元のための方法
AU1551701A (en) 1999-12-03 2001-06-12 Kaneka Corporation Novel carbonyl reductase, gene thereof and method of using the same
US6884607B2 (en) 2000-12-07 2005-04-26 Sumitomo Chemical Company, Limited Process for producing optically active 4-halo-3-hydroxybutanoate
JP4039037B2 (ja) * 2001-06-11 2008-01-30 住友化学株式会社 還元酵素遺伝子及びその利用
MXPA06001719A (es) 2003-08-11 2006-05-19 Codexis Inc Polipeptidos de glucosa deshidrogenasa mejorados, y polinucleotidos relacionados.

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2004015132A2 (en) * 2002-08-09 2004-02-19 Codexis, Inc. Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives
US7132267B2 (en) * 2002-08-09 2006-11-07 Codexis, Inc. Enzymatic processes for the production of 4-substituted 3-hydroxybutyric acid derivatives and vicinal cyano, hydroxy substituted carboxylic acid esters

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Manjon et al Biotechnology Letters 2002 Vol 24 pp 1227-32 *

Also Published As

Publication number Publication date
CA2535147A1 (en) 2005-05-19
US20050095619A1 (en) 2005-05-05
WO2005045016A2 (en) 2005-05-19
EP1660648A2 (en) 2006-05-31
US7816111B2 (en) 2010-10-19
KR20060064620A (ko) 2006-06-13
MXPA06001719A (es) 2006-05-19
WO2005045016A3 (en) 2005-11-10
EP1660648B1 (en) 2013-10-09
NO20060543L (no) 2006-03-13
IL173260A0 (en) 2006-06-11
BRPI0413492A (pt) 2006-10-17
US7939309B2 (en) 2011-05-10
US20100304459A1 (en) 2010-12-02
AU2004288134A1 (en) 2005-05-19
JP2007502114A (ja) 2007-02-08

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MK14 Patent ceased section 143(a) (annual fees not paid) or expired