ATE78055T1 - Verfahren zur herstellung von proteinen und transformierten zellen und dna-konstruktionen zur korrektur von mankos und wirtszellen sowie deren herstellung und verwendung. - Google Patents

Verfahren zur herstellung von proteinen und transformierten zellen und dna-konstruktionen zur korrektur von mankos und wirtszellen sowie deren herstellung und verwendung.

Info

Publication number
ATE78055T1
ATE78055T1 AT85303702T AT85303702T ATE78055T1 AT E78055 T1 ATE78055 T1 AT E78055T1 AT 85303702 T AT85303702 T AT 85303702T AT 85303702 T AT85303702 T AT 85303702T AT E78055 T1 ATE78055 T1 AT E78055T1
Authority
AT
Austria
Prior art keywords
production
methods
proteins
cells
host cells
Prior art date
Application number
AT85303702T
Other languages
English (en)
Inventor
Glenn Kawasaki
Leslie Bell
Original Assignee
Zymogenetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27087316&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ATE78055(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Zymogenetics Inc filed Critical Zymogenetics Inc
Application granted granted Critical
Publication of ATE78055T1 publication Critical patent/ATE78055T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C07K14/395—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts from Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/49—Platelet-derived growth factor [PDGF]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
    • C07K14/8121—Serpins
    • C07K14/8125—Alpha-1-antitrypsin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/11011—Alpharetrovirus, e.g. avian leucosis virus
    • C12N2740/11022—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Toxicology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
AT85303702T 1984-05-25 1985-05-24 Verfahren zur herstellung von proteinen und transformierten zellen und dna-konstruktionen zur korrektur von mankos und wirtszellen sowie deren herstellung und verwendung. ATE78055T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US61473484A 1984-05-25 1984-05-25
US73411985A 1985-05-15 1985-05-15
EP85303702A EP0171142B2 (de) 1984-05-25 1985-05-24 Verfahren zur Herstellung von Proteinen und transformierten Zellen und DNA-Konstruktionen zur Korrektur von Mankos und Wirtszellen sowie deren Herstellung und Verwendung

Publications (1)

Publication Number Publication Date
ATE78055T1 true ATE78055T1 (de) 1992-07-15

Family

ID=27087316

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85303702T ATE78055T1 (de) 1984-05-25 1985-05-24 Verfahren zur herstellung von proteinen und transformierten zellen und dna-konstruktionen zur korrektur von mankos und wirtszellen sowie deren herstellung und verwendung.

Country Status (8)

Country Link
US (2) US5527668A (de)
EP (1) EP0171142B2 (de)
JP (2) JP2543497B2 (de)
AT (1) ATE78055T1 (de)
AU (1) AU600885B2 (de)
CA (1) CA1285505C (de)
DE (1) DE3586304T3 (de)
DK (1) DK175105B1 (de)

Families Citing this family (47)

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AU600885B2 (en) * 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs
US5723292A (en) * 1984-05-25 1998-03-03 Zymogenetics, Inc. Stable DNA constructs
US4801542A (en) * 1984-10-12 1989-01-31 Zymogenetics, Inc. Expression of biologically active PDGF analogs in eucaryotic cells
EP0487116B1 (de) * 1984-10-12 1999-12-29 ZymoGenetics, Inc. Von Blutplättchen abstammende biologisch aktive Analoga eines Wachstumsfaktors in eukaryotischen Zellen
DK594084A (da) * 1984-12-12 1986-06-13 Novo Industri As Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
US4935349A (en) * 1986-01-17 1990-06-19 Zymogenetics, Inc. Expression of higher eucaryotic genes in aspergillus
EP0251579A3 (de) * 1986-06-24 1989-03-22 Enterovax Research Pty. Ltd. Markiersystem ohne Antibiotika
ATE131532T1 (de) * 1986-08-13 1995-12-15 Zymogenetics Inc Expression von biologisch-aktiven pdgf-analogen in eukaryotischen zellen
US5474982A (en) * 1986-08-13 1995-12-12 Zymogenetics, Inc. PDGF analogs and methods of use
DK175243B1 (da) * 1987-03-23 2004-07-19 Zymogenetics Inc Ekspressionsvektor, der er i stand til at styre ekspression af heterologe gener eller cDNA i gær, gærværtscelle samt fremgangsmåde til öget produktion af proteiner i gærværtsceller
IL87737A (en) 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
US5135854A (en) * 1987-10-29 1992-08-04 Zymogenetics, Inc. Methods of regulating protein glycosylation
WO1989006694A1 (en) * 1988-01-15 1989-07-27 Trustees Of The University Of Pennsylvania Process for selection of proteinaceous substances which mimic growth-inducing molecules
JP3273609B2 (ja) * 1989-07-07 2002-04-08 ユニリーバー・ナームローゼ・ベンノートシヤープ 発現ベクターのマルチコピー組込みにより形質転換した真菌による蛋白質の製法
US6004776A (en) * 1989-07-07 1999-12-21 Unilever Patent Holdings, B.V. Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
US5759833A (en) * 1994-05-27 1998-06-02 Cubist Pharmaceuticals, Inc. Human isoleucyl-tRNA synthetase proteins, nucleic acids and tester strains comprising same
GB2318732A (en) 1996-11-01 1998-05-06 Johnson & Johnson Medical Wound healing compositions containing alpha-1-antitrypsin
US20020119579A1 (en) * 1998-07-14 2002-08-29 Peter Wagner Arrays devices and methods of use thereof
US6576478B1 (en) * 1998-07-14 2003-06-10 Zyomyx, Inc. Microdevices for high-throughput screening of biomolecules
US6406921B1 (en) 1998-07-14 2002-06-18 Zyomyx, Incorporated Protein arrays for high-throughput screening
US6897073B2 (en) * 1998-07-14 2005-05-24 Zyomyx, Inc. Non-specific binding resistant protein arrays and methods for making the same
DE60044728D1 (de) 1999-12-29 2010-09-02 Novo Nordisk As VERFAHREN ZUR HERSTELLUNG VON INSULINVORLäUFERN UND ANALOGEN VON INSULINVORLÄUFERN MIT VERBESSERTER FERMENTATIONSAUSBEUTE IN HEFE
US7009045B2 (en) * 2000-07-14 2006-03-07 Archer-Daniels-Midland Company Transformation systems for flavinogenic yeast
JP3966819B2 (ja) 2001-05-24 2007-08-29 キム，スーギョン 毛嚢に存在する新規の第２ケラチノサイト成長因子類似体
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
EP2314629B2 (de) 2002-07-18 2022-11-16 Merus N.V. Rekombinante Herstellung von Mischungen aus Aantikörpern
USRE47770E1 (en) 2002-07-18 2019-12-17 Merus N.V. Recombinant production of mixtures of antibodies
US20100069614A1 (en) 2008-06-27 2010-03-18 Merus B.V. Antibody producing non-human mammals
CA2527694C (en) 2003-05-30 2015-07-14 Hendricus Renerus Jacobus Mattheus Hoogenboom Fab library for the preparation of anti vegf and anti rabies virus fabs
EP1687428A1 (de) 2003-11-14 2006-08-09 Novo Nordisk A/S Verfahren zur herstellung von acyliertem insulin
EP1692168B1 (de) 2003-12-03 2011-07-20 Novo Nordisk A/S Einzelketteninsulin
JP2006067883A (ja) * 2004-09-01 2006-03-16 Kao Corp 組換えバチルス属細菌
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EP1831375B1 (de) 2004-12-23 2014-07-16 Novozymes Biopharma DK A/S Genexpressionsmethode
ES2368500T3 (es) 2005-08-16 2011-11-17 Novo Nordisk A/S Método para producir polipéptidos de insulina maduros.
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AU2007278994B2 (en) 2006-07-24 2013-08-15 Biorexis Pharmaceutical Corporation Exendin fusion proteins
JP5503968B2 (ja) 2006-09-27 2014-05-28 ノボ・ノルデイスク・エー／エス 成熟インスリンポリペプチドの作製方法
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US8575096B2 (en) 2007-08-13 2013-11-05 Novo Nordisk A/S Rapid acting insulin analogues
EP2178910B1 (de) 2007-08-15 2014-10-08 Novo Nordisk A/S Insuline mit einer acyl-einheit mit verstärkungseinheiten aus alkylenglycol mit aminosäuren
JP5730569B2 (ja) 2007-08-15 2015-06-10 ノボ・ノルデイスク・エー／エス アシル及びアルキレングリコール部分を有するインスリンアナログ
WO2011089170A2 (en) 2010-01-22 2011-07-28 Novo Nordisk A/S Process for preparing fgf-21 with low degree of o-glycosylation
US8815541B2 (en) 2009-11-25 2014-08-26 Novo Nordisk A/S Method for making polypeptides
JP6393255B2 (ja) 2012-04-20 2018-09-19 メルス ナムローゼ フェンノートシャップ ヘテロ二量体のＩｇＧ様分子を生産する方法、ヘテロ二量体のＩｇＧ様分子、ヘテロ二量体の抗体、組み換え宿主細胞、医薬組成物、宿主細胞を作成する方法、および培養物
WO2014195452A1 (en) 2013-06-07 2014-12-11 Novo Nordisk A/S Method for making mature insulin polypeptides
KR102277898B1 (ko) * 2014-07-03 2021-07-15 삼성전자주식회사 산물 생산능이 향상된 효모 및 그를 이용한 산물을 생산하는 방법

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Also Published As

Publication number Publication date
AU4279485A (en) 1985-11-28
US5527668A (en) 1996-06-18
JP2543497B2 (ja) 1996-10-16
DK235085D0 (da) 1985-05-24
US5700643A (en) 1997-12-23
JP2704115B2 (ja) 1998-01-26
AU600885B2 (en) 1990-08-30
DE3586304T2 (de) 1992-12-10
DK235085A (da) 1985-11-26
DE3586304T3 (de) 2005-02-17
EP0171142B1 (de) 1992-07-08
EP0171142A1 (de) 1986-02-12
CA1285505C (en) 1991-07-02
DK175105B1 (da) 2004-06-01
JPS6156092A (ja) 1986-03-20
DE3586304D1 (de) 1992-08-13
JPH0767685A (ja) 1995-03-14
EP0171142B2 (de) 2004-09-01

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