ATE55413T1 - Verfahren zur stabilisierung extrachromosomaler elemente in bakterien waehrend deren zuechtung, extrachromosomale elemente, transformierte bakterien und verfahren zur herstellung eines gewuenschten produktes in transformierten bakterien. - Google Patents

Verfahren zur stabilisierung extrachromosomaler elemente in bakterien waehrend deren zuechtung, extrachromosomale elemente, transformierte bakterien und verfahren zur herstellung eines gewuenschten produktes in transformierten bakterien.

Info

Publication number
ATE55413T1
ATE55413T1 AT85309020T AT85309020T ATE55413T1 AT E55413 T1 ATE55413 T1 AT E55413T1 AT 85309020 T AT85309020 T AT 85309020T AT 85309020 T AT85309020 T AT 85309020T AT E55413 T1 ATE55413 T1 AT E55413T1
Authority
AT
Austria
Prior art keywords
bacteria
chromosomal
gene
extra
methods
Prior art date
Application number
AT85309020T
Other languages
English (en)
Inventor
Boerge Krag Diderichsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE55413T1 publication Critical patent/ATE55413T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Biological Depolymerization Polymers (AREA)
  • Saccharide Compounds (AREA)
  • Mushroom Cultivation (AREA)
  • Discharge Heating (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
AT85309020T 1984-12-12 1985-12-11 Verfahren zur stabilisierung extrachromosomaler elemente in bakterien waehrend deren zuechtung, extrachromosomale elemente, transformierte bakterien und verfahren zur herstellung eines gewuenschten produktes in transformierten bakterien. ATE55413T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK594084A DK594084A (da) 1984-12-12 1984-12-12 Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
EP85309020A EP0185512B1 (de) 1984-12-12 1985-12-11 Verfahren zur Stabilisierung extrachromosomaler Elemente in Bakterien während deren Züchtung, extrachromosomale Elemente, transformierte Bakterien und Verfahren zur Herstellung eines gewünschten Produktes in transformierten Bakterien

Publications (1)

Publication Number Publication Date
ATE55413T1 true ATE55413T1 (de) 1990-08-15

Family

ID=8146313

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85309020T ATE55413T1 (de) 1984-12-12 1985-12-11 Verfahren zur stabilisierung extrachromosomaler elemente in bakterien waehrend deren zuechtung, extrachromosomale elemente, transformierte bakterien und verfahren zur herstellung eines gewuenschten produktes in transformierten bakterien.

Country Status (10)

Country Link
US (1) US4920048A (de)
EP (1) EP0185512B1 (de)
JP (2) JP2576970B2 (de)
AT (1) ATE55413T1 (de)
AU (1) AU592542B2 (de)
CA (1) CA1292197C (de)
DE (1) DE3579133D1 (de)
DK (1) DK594084A (de)
FI (1) FI85876C (de)
NO (1) NO172063C (de)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5198343A (en) * 1986-08-05 1993-03-30 Transgene, S.A. Method for expressing a heterologous protein in a dapD- mutant of E. coli and the strain obtained
FR2602792B1 (fr) * 1986-08-05 1989-10-20 Transgene Sa Procede de stabilisation d'un plasmide contenu dans une souche bacterienne et souche obtenue
FR2618159B2 (fr) * 1987-07-15 1990-02-02 Transgene Sa Procede de stabilisation d'un plasmide contenu dans une souche bacterienne et souche obtenue
DE3751564T2 (de) * 1986-08-05 1996-03-28 Transgene Sa Verfahren zur Stabilisierung eines Plasmids, das in einem Bakterienstamm enthalten ist und der erhaltene Stamm.
JPH04501351A (ja) * 1987-10-07 1992-03-12 ワシントン ユニバーシティ 細胞の遺伝的集団において所望の組換え遺伝子を維持する方法
HU200796B (en) * 1988-07-14 1990-08-28 Richter Gedeon Vegyeszet Process for producing expression vectors ensuring insertion of nucleotide sequences into e. coli chromosome
RU2099418C1 (ru) * 1991-11-21 1997-12-20 Лонца Аг Фрагмент днк, обеспечивающий использование бетаина, рекомбинантная плазмидная днк с фрагментом днк, обеспечивающим использование бетаина, штамм бактерий rhizobium/agrobacterium, предназначенный для стабильного хранения плазмиды с фрагментом днк, способ получения штамма бактерий
PL307505A1 (en) * 1992-08-18 1995-05-29 Duphar Int Res Vaccine against bordetella bronchiseptica
US5714375A (en) * 1995-06-05 1998-02-03 Nobl Laboratories, Inc. Ileal symbiont intracellularis propagation in suspended host cells
US5885823A (en) * 1995-06-05 1999-03-23 Nobl Laboratories, Inc. Lawsonia intracellularis cultivation, anti-Lawsonia intracellularis vaccines and diagnostic agents
US6743780B1 (en) 1995-09-08 2004-06-01 Cobra Biologics Limited Plasmid stabilization
GB9518395D0 (en) * 1995-09-08 1995-11-08 Therexsys Ltd Plasmid stabilization
US5922583A (en) * 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
US6291245B1 (en) 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
EP0972838B1 (de) * 1998-07-15 2004-09-15 Roche Diagnostics GmbH Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie
JP3958884B2 (ja) 1998-09-11 2007-08-15 株式会社林原生物化学研究所 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
JP2004501651A (ja) 2000-06-23 2004-01-22 ノボザイムス　アクティーゼルスカブ 遺伝子の安定した染色体多コピー組み込みのための方法
CN1322138C (zh) * 2000-06-23 2007-06-20 诺维信公司 用于基因的稳定的染色体多拷贝整合的方法
JP5087741B2 (ja) * 2003-11-19 2012-12-05 フェネックス インコーポレイテッド 改良タンパク質発現系
JP2006067883A (ja) * 2004-09-01 2006-03-16 Kao Corp 組換えバチルス属細菌
JP2006067884A (ja) * 2004-09-01 2006-03-16 Kao Corp 組換えバチルス属細菌
US7923231B2 (en) * 2004-12-17 2011-04-12 Cargill, Incorporated Production of glucuronic acid using myo-inositol oxygenase from cryptococcus neoformans
US8834891B2 (en) * 2005-03-14 2014-09-16 Boehringer Ingelheim Vetmedica, Inc. Immunogenic compositions comprising Lawsonia intracellularis
US8398994B2 (en) * 2005-07-15 2013-03-19 Boehringer Ingelheim Vetmedica, Inc. Lawsonia vaccine and methods of use thereof
NZ567190A (en) * 2005-10-06 2012-07-27 Dompe Pha R Ma Spa Res & Mfg Method of producing a recombinant protein comprising transforming an E. Coli host cell lacking a gene encoding pyrC with a vector comprising a gene encoding the recombinant protein and a gene encoding E. coli pyrC
US8470336B2 (en) * 2006-05-25 2013-06-25 Boehringer Ingelheim Vetmedica, Inc. Vaccination of young animals against Lawsonia intracellularis infections
DE102007021001A1 (de) 2007-05-04 2008-11-06 Ab Enzymes Gmbh Expressionssystem zur antibiotikafreien Produktion von Polypeptiden
EP2200643B1 (de) 2007-09-17 2013-09-04 Boehringer Ingelheim Vetmedica, Inc. Ein lawsonia intracellularis lebendimpfstoff verabreicht in kombination mit einem antibiotikum zur behandlung von lawsonia intracellularis infektionen in schweine
NZ596283A (en) 2009-05-11 2013-08-30 Pfenex Inc Production of recombinant proteins utilizing non-antibiotic selection methods and the incorporation of non-natural amino acids therein
DK3387111T3 (da) 2015-12-11 2021-05-10 Wacker Chemie Ag Mikroorganismestamme og fremgangsmåde til antibiotikafri, fermentativ fremstilling af lavmolekylære substanser og proteiner
DK3584316T1 (da) 2018-06-20 2020-08-03 Eligo Bioscience Bakteriel leveringsvehikel, fremgangsmåde til fremstilling og anvendelser deraf
US12098372B2 (en) 2019-12-30 2024-09-24 Eligo Bioscience Microbiome modulation of a host by delivery of DNA payloads with minimized spread
US11746352B2 (en) 2019-12-30 2023-09-05 Eligo Bioscience Microbiome modulation of a host by delivery of DNA payloads with minimized spread
WO2022144382A1 (en) 2020-12-30 2022-07-07 Eligo Bioscience Chimeric receptor binding proteins resistant to proteolytic degradation
CA3219077A1 (en) 2021-05-12 2022-11-17 Eligo Bioscience Production of lytic phages
GB202304540D0 (en) 2023-03-28 2023-05-10 Univ Tartu Method of biosynthesis

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE48385B1 (en) * 1978-08-11 1984-12-26 Univ California Synthesis of a eucaryotic protein by a microorganism
US4506013A (en) * 1980-10-03 1985-03-19 Eli Lilly And Company Stabilizing and selecting recombinant DNA host cells
US4436815A (en) * 1981-11-27 1984-03-13 Eli Lilly And Company Method for stabilizing and selecting recombinant DNA containing host cells
DK410783D0 (da) * 1982-09-16 1983-09-09 Benzon A Salfred Fremgangsmade til stabilisering af plasmider
US4595660A (en) * 1983-03-02 1986-06-17 University Of Delaware Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis, mutants and substantially stably transformed mutants of Bacillus subtilis, and methods for utilizing the transformed mutants
JPS59205983A (ja) * 1983-04-28 1984-11-21 ジエネツクス・コ−ポレイシヨン 異種遺伝子を原核微生物で発現させる方法
AU600885B2 (en) * 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs

Also Published As

Publication number Publication date
NO172063C (no) 1993-06-02
FI854893A7 (fi) 1986-06-13
US4920048A (en) 1990-04-24
AU5108185A (en) 1986-06-19
EP0185512A1 (de) 1986-06-25
NO172063B (no) 1993-02-22
NO854966L (no) 1986-06-13
JP2571338B2 (ja) 1997-01-16
JP2576970B2 (ja) 1997-01-29
FI85876B (fi) 1992-02-28
AU592542B2 (en) 1990-01-18
EP0185512B1 (de) 1990-08-08
JPS61139392A (ja) 1986-06-26
FI854893A0 (fi) 1985-12-11
DK594084D0 (da) 1984-12-12
CA1292197C (en) 1991-11-19
DE3579133D1 (de) 1990-09-13
DK594084A (da) 1986-06-13
JPH0686669A (ja) 1994-03-29
FI85876C (fi) 1992-06-10

Similar Documents

Publication Publication Date Title
DE3579133D1 (de) Verfahren zur stabilisierung extrachromosomaler elemente in bakterien waehrend deren zuechtung, extrachromosomale elemente, transformierte bakterien und verfahren zur herstellung eines gewuenschten produktes in transformierten bakterien.
DE3486229T2 (de) Verfahren zur Herstellung von L-Phenylalanin.
FI841977A7 (fi) Stabiliserade plasmider
ES8304206A1 (es) "procedimiento para disociar adn de doble hebra en cualquier lugar establecido".
EP0088195A3 (de) Messenger-RNS dessen Herstellung und Verwendung
ES2104608T3 (es) Procedimiento para la produccion de una composicion que contiene zeaxantina utilizando un microorganismo de la especie flavobacterium multivorum.
DE69330518D1 (de) Dna, die aspartokinase iii-mutanten kodiert und ihre verwendung zur herstellung von l-threonin durch fermentation
DE3878874D1 (de) Verfahren zur herstellung von antibiotischem pulver enthaltenden dispersionen.
PT76578B (de) Mikrobielle polysaccharide verfahren zu ihrer herstellung dafuer geeignete mikroorganismen und verwendung der polysaccharide
AU667869B2 (en) Method of producing plastics containing amide groups
JPS55131397A (en) Preparation of l-threonine by fermentation
ATE353953T1 (de) Wirtszelle, die reduzierte mengen einer metalloprotease exprimiert und methoden zur verwendung dieser wirtszelle zur proteinproduktion
DE59611206D1 (de) Thermolabile uracil-dna-glykosylase, verfahren zu deren herstellung und verwendung zur entfernung von uracil aus dna
PT677110E (pt) Bacteria lactica recombinante contendo um promotor inserido
DE69325449D1 (de) Verfahren zur entwicklung von neuen pflanzentypen mit kapazität zur stickstoffbindung ebenfalls in deren blättern
GB1588572A (en) Process for the production of filamentous hybrid phages
IL101129A0 (en) Biotechnological process for the preparation of s-(+)-2,2-dimethylcyclopropanecarboxamide
DK571985A (da) Plasmidstabilisering
DE3586410D1 (de) Verfahren zur herstellung von neutraler protease.
ES2074509T3 (es) Metodo para cultivar protoplastos de tomate.
RU2206612C2 (ru) Способ получения шикимовой кислоты (варианты), штамм бактерий bacillus subtilis - продуцент шикимовой кислоты (варианты)
EP0193883A3 (en) Well forming apparatus
DE69231145D1 (de) Für eine Esterase kodierendes Gen und dieses Gen enthaltender Mikroorganismus
ATE95568T1 (de) Mikrobiologisches verfahren zur herstellung von uk-61.689 und mikroorganismen zur verwendung darin.
ATE2339T1 (de) Verfahren zur herstellung von sphingomyelinase, sphingomyelinase und deren verwendung.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee