ATE55413T1 - Verfahren zur stabilisierung extrachromosomaler elemente in bakterien waehrend deren zuechtung, extrachromosomale elemente, transformierte bakterien und verfahren zur herstellung eines gewuenschten produktes in transformierten bakterien. - Google Patents

Verfahren zur stabilisierung extrachromosomaler elemente in bakterien waehrend deren zuechtung, extrachromosomale elemente, transformierte bakterien und verfahren zur herstellung eines gewuenschten produktes in transformierten bakterien.

Info

Publication number
ATE55413T1
ATE55413T1 AT85309020T AT85309020T ATE55413T1 AT E55413 T1 ATE55413 T1 AT E55413T1 AT 85309020 T AT85309020 T AT 85309020T AT 85309020 T AT85309020 T AT 85309020T AT E55413 T1 ATE55413 T1 AT E55413T1
Authority
AT
Austria
Prior art keywords
bacteria
chromosomal
gene
extra
methods
Prior art date
Application number
AT85309020T
Other languages
English (en)
Inventor
Boerge Krag Diderichsen
Original Assignee
Novo Nordisk As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novo Nordisk As filed Critical Novo Nordisk As
Application granted granted Critical
Publication of ATE55413T1 publication Critical patent/ATE55413T1/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Biological Depolymerization Polymers (AREA)
  • Food Preservation Except Freezing, Refrigeration, And Drying (AREA)
  • Mushroom Cultivation (AREA)
  • Discharge Heating (AREA)
  • Polymers With Sulfur, Phosphorus Or Metals In The Main Chain (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
AT85309020T 1984-12-12 1985-12-11 Verfahren zur stabilisierung extrachromosomaler elemente in bakterien waehrend deren zuechtung, extrachromosomale elemente, transformierte bakterien und verfahren zur herstellung eines gewuenschten produktes in transformierten bakterien. ATE55413T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK594084A DK594084A (da) 1984-12-12 1984-12-12 Fremgangsmaade til stabilisering af extra-chromosomale elementer i bakterier under dyrkning
EP85309020A EP0185512B1 (de) 1984-12-12 1985-12-11 Verfahren zur Stabilisierung extrachromosomaler Elemente in Bakterien während deren Züchtung, extrachromosomale Elemente, transformierte Bakterien und Verfahren zur Herstellung eines gewünschten Produktes in transformierten Bakterien

Publications (1)

Publication Number Publication Date
ATE55413T1 true ATE55413T1 (de) 1990-08-15

Family

ID=8146313

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85309020T ATE55413T1 (de) 1984-12-12 1985-12-11 Verfahren zur stabilisierung extrachromosomaler elemente in bakterien waehrend deren zuechtung, extrachromosomale elemente, transformierte bakterien und verfahren zur herstellung eines gewuenschten produktes in transformierten bakterien.

Country Status (10)

Country Link
US (1) US4920048A (de)
EP (1) EP0185512B1 (de)
JP (2) JP2576970B2 (de)
AT (1) ATE55413T1 (de)
AU (1) AU592542B2 (de)
CA (1) CA1292197C (de)
DE (1) DE3579133D1 (de)
DK (1) DK594084A (de)
FI (1) FI85876C (de)
NO (1) NO172063C (de)

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US5198343A (en) * 1986-08-05 1993-03-30 Transgene, S.A. Method for expressing a heterologous protein in a dapD- mutant of E. coli and the strain obtained
FR2618159B2 (fr) * 1987-07-15 1990-02-02 Transgene Sa Procede de stabilisation d'un plasmide contenu dans une souche bacterienne et souche obtenue
DK408687A (da) * 1986-08-05 1988-02-06 Transgene Sa Fremgangsmaade til stabilisering af et plasmid
FR2602792B1 (fr) * 1986-08-05 1989-10-20 Transgene Sa Procede de stabilisation d'un plasmide contenu dans une souche bacterienne et souche obtenue
ATE121785T1 (de) * 1987-10-07 1995-05-15 Univ Washington Verfahren zur erhaltung eines erwünschten rekombinanten gens in einer genetischen zellpopulation.
HU200796B (en) * 1988-07-14 1990-08-28 Richter Gedeon Vegyeszet Process for producing expression vectors ensuring insertion of nucleotide sequences into e. coli chromosome
RU2099418C1 (ru) * 1991-11-21 1997-12-20 Лонца Аг Фрагмент днк, обеспечивающий использование бетаина, рекомбинантная плазмидная днк с фрагментом днк, обеспечивающим использование бетаина, штамм бактерий rhizobium/agrobacterium, предназначенный для стабильного хранения плазмиды с фрагментом днк, способ получения штамма бактерий
HU219819B (hu) * 1992-08-18 2001-08-28 Dimminaco Ag/Sa/Ltd. Izolált polinukleotidok, ezeket tartalmazó Bordetella bronchiseptica törzs, és Bordetella bronchiseptica elleni vakcina
US5714375A (en) * 1995-06-05 1998-02-03 Nobl Laboratories, Inc. Ileal symbiont intracellularis propagation in suspended host cells
US5885823A (en) * 1995-06-05 1999-03-23 Nobl Laboratories, Inc. Lawsonia intracellularis cultivation, anti-Lawsonia intracellularis vaccines and diagnostic agents
US6743780B1 (en) 1995-09-08 2004-06-01 Cobra Biologics Limited Plasmid stabilization
GB9518395D0 (en) * 1995-09-08 1995-11-08 Therexsys Ltd Plasmid stabilization
US5922583A (en) * 1995-10-17 1999-07-13 Biostar Inc. Methods for production of recombinant plasmids
EP0972838B1 (de) * 1998-07-15 2004-09-15 Roche Diagnostics GmbH Escherichia coli Wirt/Vektorsystem basierend auf einer antibiotikafreien Selektion durch Komplementation einer Auxotrophie
US6291245B1 (en) 1998-07-15 2001-09-18 Roche Diagnostics Gmbh Host-vector system
JP3958884B2 (ja) 1998-09-11 2007-08-15 株式会社林原生物化学研究所 非還元性糖質生成酵素及びトレハロース遊離酵素ならびに該酵素を用いる糖質の製造方法
DK1297170T3 (en) * 2000-06-23 2016-03-14 Novozymes As A process for stable chromosomal integration of multiple copies of the genes
AU2001265835A1 (en) 2000-06-23 2002-01-08 Novozymes A/S Method for stable chromosomal multi-copy integration of genes
CA2545610C (en) * 2003-11-19 2014-03-25 Dow Global Technolgies Inc. Auxotrophic pseudomonas fluorescens bacteria for recombinant protein expression
JP2006067883A (ja) * 2004-09-01 2006-03-16 Kao Corp 組換えバチルス属細菌
JP2006067884A (ja) * 2004-09-01 2006-03-16 Kao Corp 組換えバチルス属細菌
US7923231B2 (en) * 2004-12-17 2011-04-12 Cargill, Incorporated Production of glucuronic acid using myo-inositol oxygenase from cryptococcus neoformans
US8834891B2 (en) * 2005-03-14 2014-09-16 Boehringer Ingelheim Vetmedica, Inc. Immunogenic compositions comprising Lawsonia intracellularis
US8398994B2 (en) * 2005-07-15 2013-03-19 Boehringer Ingelheim Vetmedica, Inc. Lawsonia vaccine and methods of use thereof
ES2562664T3 (es) * 2005-10-06 2016-03-07 Advanced Accelerator Applications S.A. Nuevo sistema de selección
US8470336B2 (en) * 2006-05-25 2013-06-25 Boehringer Ingelheim Vetmedica, Inc. Vaccination of young animals against Lawsonia intracellularis infections
DE102007021001A1 (de) 2007-05-04 2008-11-06 Ab Enzymes Gmbh Expressionssystem zur antibiotikafreien Produktion von Polypeptiden
US8398970B2 (en) * 2007-09-17 2013-03-19 Boehringer Ingelheim Vetmedica, Inc. Method of preventing early Lawsonia intracellularis infections
EP2430164B1 (de) 2009-05-11 2017-07-19 Pfenex, Inc. Herstellung von rekombinanten proteinen anhand von nicht-antibiotischen auswahlverfahren und einbau von nicht-natürlichen aminosäuren
ES2867964T3 (es) 2015-12-11 2021-10-21 Wacker Chemie Ag Cepa de microorganismos y procedimiento para la producción fermentativa exenta de antibióticos de sustancias y proteínas de bajo peso molecular
EP3584316B1 (de) 2018-06-20 2024-04-03 Eligo Bioscience Bakterielles freisetzungsvehikel, verfahren zur herstellung und verwendungen davon
US12098372B2 (en) 2019-12-30 2024-09-24 Eligo Bioscience Microbiome modulation of a host by delivery of DNA payloads with minimized spread
US11746352B2 (en) 2019-12-30 2023-09-05 Eligo Bioscience Microbiome modulation of a host by delivery of DNA payloads with minimized spread
WO2022144381A1 (en) 2020-12-30 2022-07-07 Eligo Bioscience Microbiome modulation of a host by delivery of dna payloads with minimized spread
KR20240020271A (ko) 2021-05-12 2024-02-14 엘리고 바이오사이언스 생산 박테리아 세포 및 제조 방법에서 이의 용도
GB202304540D0 (en) 2023-03-28 2023-05-10 Univ Tartu Method of biosynthesis

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IE48385B1 (en) * 1978-08-11 1984-12-26 Univ California Synthesis of a eucaryotic protein by a microorganism
US4506013A (en) * 1980-10-03 1985-03-19 Eli Lilly And Company Stabilizing and selecting recombinant DNA host cells
US4436815A (en) * 1981-11-27 1984-03-13 Eli Lilly And Company Method for stabilizing and selecting recombinant DNA containing host cells
DK410783D0 (da) * 1982-09-16 1983-09-09 Benzon A Salfred Fremgangsmade til stabilisering af plasmider
US4595660A (en) * 1983-03-02 1986-06-17 University Of Delaware Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis, mutants and substantially stably transformed mutants of Bacillus subtilis, and methods for utilizing the transformed mutants
JPS59205983A (ja) * 1983-04-28 1984-11-21 ジエネツクス・コ−ポレイシヨン 異種遺伝子を原核微生物で発現させる方法
AU600885B2 (en) * 1984-05-25 1990-08-30 Zymogenetics Inc. Stable DNA constructs

Also Published As

Publication number Publication date
AU592542B2 (en) 1990-01-18
AU5108185A (en) 1986-06-19
EP0185512A1 (de) 1986-06-25
DK594084D0 (da) 1984-12-12
NO172063B (no) 1993-02-22
FI854893A7 (fi) 1986-06-13
JPS61139392A (ja) 1986-06-26
NO172063C (no) 1993-06-02
FI85876B (fi) 1992-02-28
FI85876C (fi) 1992-06-10
NO854966L (no) 1986-06-13
DK594084A (da) 1986-06-13
JP2576970B2 (ja) 1997-01-29
EP0185512B1 (de) 1990-08-08
JPH0686669A (ja) 1994-03-29
FI854893A0 (fi) 1985-12-11
US4920048A (en) 1990-04-24
JP2571338B2 (ja) 1997-01-16
CA1292197C (en) 1991-11-19
DE3579133D1 (de) 1990-09-13

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