ATE552500T1 - Lipophile, farbstoffbasierte fret-assays für die clostridientoxinaktivität - Google Patents
Lipophile, farbstoffbasierte fret-assays für die clostridientoxinaktivitätInfo
- Publication number
- ATE552500T1 ATE552500T1 AT10164461T AT10164461T ATE552500T1 AT E552500 T1 ATE552500 T1 AT E552500T1 AT 10164461 T AT10164461 T AT 10164461T AT 10164461 T AT10164461 T AT 10164461T AT E552500 T1 ATE552500 T1 AT E552500T1
- Authority
- AT
- Austria
- Prior art keywords
- clostridial toxin
- clostridial
- toxinactivity
- lipophilic
- dye
- Prior art date
Links
- 238000002866 fluorescence resonance energy transfer Methods 0.000 title abstract 3
- 230000007888 toxin activity Effects 0.000 title abstract 3
- 241001112695 Clostridiales Species 0.000 title 1
- 238000003556 assay Methods 0.000 title 1
- 231100001102 clostridial toxin Toxicity 0.000 abstract 5
- 239000012528 membrane Substances 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 238000003776 cleavage reaction Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 230000007017 scission Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5058—Neurological cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
- G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56911—Bacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/94—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving narcotics or drugs or pharmaceuticals, neurotransmitters or associated receptors
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Toxicology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Pharmacology & Pharmacy (AREA)
- Virology (AREA)
- Neurology (AREA)
- Neurosurgery (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Glass Compositions (AREA)
- Valve-Gear Or Valve Arrangements (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US66894205P | 2005-04-05 | 2005-04-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE552500T1 true ATE552500T1 (de) | 2012-04-15 |
Family
ID=36975328
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT10164461T ATE552500T1 (de) | 2005-04-05 | 2006-04-04 | Lipophile, farbstoffbasierte fret-assays für die clostridientoxinaktivität |
| AT06749210T ATE470857T1 (de) | 2005-04-05 | 2006-04-04 | Lipophile, farbstoffbasierte fret-assays für die clostridientoxinaktivität |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT06749210T ATE470857T1 (de) | 2005-04-05 | 2006-04-04 | Lipophile, farbstoffbasierte fret-assays für die clostridientoxinaktivität |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US8124357B2 (de) |
| EP (2) | EP2293064B1 (de) |
| AT (2) | ATE552500T1 (de) |
| AU (1) | AU2006231470B2 (de) |
| CA (1) | CA2604039C (de) |
| DE (1) | DE602006014812D1 (de) |
| DK (1) | DK1869459T3 (de) |
| ES (1) | ES2345506T3 (de) |
| PT (1) | PT1869459E (de) |
| WO (1) | WO2006107921A2 (de) |
Families Citing this family (36)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7374896B2 (en) * | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
| US8022172B2 (en) * | 2001-08-28 | 2011-09-20 | Allergan, Inc. | Luminescence resonance energy transfer (LRET) assays for clostridial toxin activity |
| US7332567B2 (en) * | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
| US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
| US8497081B2 (en) | 2004-02-24 | 2013-07-30 | Allergan, Inc. | Botulinum toxin screening assays |
| DK1718756T3 (da) * | 2004-02-24 | 2012-02-27 | Allergan Inc | Botulinumtoksinscreeningassays |
| ATE552500T1 (de) * | 2005-04-05 | 2012-04-15 | Allergan Inc | Lipophile, farbstoffbasierte fret-assays für die clostridientoxinaktivität |
| ATE555387T1 (de) * | 2005-10-12 | 2012-05-15 | Allergan Inc | Tests der molekularen oder subzellulären nähe unter verwendung von depolarisierung nach resonanzenergietransfer (daret) |
| WO2008140834A2 (en) | 2007-01-16 | 2008-11-20 | The Regents Of The University Of California | Novel antimicrobial peptides |
| US8198034B2 (en) | 2008-03-14 | 2012-06-12 | Allergan, Inc. | Immuno-based botulinum toxin serotype A activity assays |
| KR101604515B1 (ko) * | 2008-03-14 | 2016-03-17 | 알러간, 인코포레이티드 | 면역-기반 보툴리눔 독소 세로타입 ａ 활성 검정 |
| WO2010105236A1 (en) | 2009-03-13 | 2010-09-16 | Allergan, Inc. | Immuno-based retargeted endopeptidase activity assays |
| CN102439138B (zh) | 2009-03-13 | 2018-03-30 | 阿勒根公司 | 可用于基于免疫的肉毒杆菌毒素血清a型活性测定的细胞 |
| US8129139B2 (en) | 2009-07-13 | 2012-03-06 | Allergan, Inc. | Process for obtaining botulinum neurotoxin |
| US10246492B2 (en) | 2009-10-16 | 2019-04-02 | Biomadison, Inc. | Botulinum assay with synaptobrevin substrate moiety |
| EP2488868B1 (de) * | 2009-10-16 | 2016-03-30 | BioMadison, Inc. | Resonanzenergie-transferassay mit einem synaptobrevin-fragment als substrat |
| KR20130111954A (ko) * | 2010-06-11 | 2013-10-11 | 시냅틱 리서치, 엘엘씨 | N-단부 지배 프로테아제 활성 표시 방법 및 이의 용도 |
| JP6215223B2 (ja) * | 2011-12-09 | 2017-10-18 | アンスティテュ・パストゥール | マルチプレックス免疫スクリーニングアッセイ |
| EP2798077A2 (de) | 2011-12-31 | 2014-11-05 | Allergan, Inc. | Hochempfindlicher zellbasierter test für den nachweis der anwesenheit von aktivem botulinum-neurotoxin-serotyp-a |
| US8778623B2 (en) * | 2012-09-14 | 2014-07-15 | Wisconsin Alumni Research Foundation | Compositions and methods of using differentiated cells sensitized to botulinum neurotoxin |
| US8962340B2 (en) | 2012-12-03 | 2015-02-24 | Src, Inc. | Real-time assay for the detection of botulinum toxin |
| DE102013226511B4 (de) | 2013-12-18 | 2016-12-15 | TRUMPF Hüttinger GmbH + Co. KG | Leistungsversorgungssystem und Verfahren zur Erzeugung einer Leistung |
| DE102013226537B4 (de) | 2013-12-18 | 2022-12-29 | TRUMPF Hüttinger GmbH + Co. KG | Leistungsversorgungssystem mit mehreren Verstärkerpfaden sowie Verfahren zur Anregung eines Plasmas |
| EP3567055A1 (de) | 2014-07-07 | 2019-11-13 | Allergan, Inc. | Verfahren zum nachweis von gespaltenem snap25 in gewebeproben |
| US9788776B1 (en) | 2014-09-22 | 2017-10-17 | Verily Life Sciences Llc | Protein M-based in vivo diagnostic system and detection method |
| US9927442B1 (en) | 2014-10-31 | 2018-03-27 | Verily Life Sciences Llc | Biosensor for in vitro detection system and method of use |
| AU2015367329B2 (en) | 2014-12-19 | 2021-02-18 | Merz Pharma Gmbh & Co. Kgaa | Means and methods for the determination of the biological activity of BoNT/E in cells |
| US10676723B2 (en) | 2015-05-11 | 2020-06-09 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| EP3458600A4 (de) | 2016-05-16 | 2020-01-15 | BioMadison, Inc. | Verbesserter test mit teil auf der basis von synaptobrevin |
| IL265109B2 (en) | 2016-09-13 | 2024-05-01 | Allergan Inc | Stabilized non-protein clostridial toxin compositions |
| US11129906B1 (en) | 2016-12-07 | 2021-09-28 | David Gordon Bermudes | Chimeric protein toxins for expression by therapeutic bacteria |
| BR112020012641A2 (pt) | 2017-12-20 | 2020-12-01 | Allergan, Inc. | polipeptídeos de domínio de ligação celular de toxina de botulinum e métodos de uso para rejuvenescimento da pele |
| AR119109A1 (es) * | 2019-06-07 | 2021-11-24 | Synaptic Res Llc | Células genéticamente manipuladas |
| US20220326221A1 (en) * | 2019-08-13 | 2022-10-13 | Synaptic Research, Llc | Cells Highly Sensitive to Clostridial Neurotoxin |
| CN110882276B (zh) * | 2019-11-23 | 2021-07-06 | 博雅干细胞科技有限公司 | 细胞治疗组合物及治疗血管病变的方法 |
| CN110840914B (zh) * | 2019-11-23 | 2021-07-06 | 博雅干细胞科技有限公司 | 细胞治疗剂用于缓解或改善血管病变的方法 |
Family Cites Families (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5965699A (en) * | 1996-11-06 | 1999-10-12 | The United States Of America As Represented By The Secretary Of The Army | Assay for the proteolytic activity of serotype a from clostridium botulinum |
| US7090976B2 (en) * | 1999-11-10 | 2006-08-15 | Rigel Pharmaceuticals, Inc. | Methods and compositions comprising Renilla GFP |
| FR2809733B1 (fr) * | 2000-06-02 | 2004-04-30 | Inst Nat Sante Rech Med | Substrat peptidique reconnu par la toxine botulique de type bont/b et son utilisation pour doser et/ou detecter ladite toxine ou des inhibiteurs correspondants |
| ATE378596T1 (de) * | 2000-09-25 | 2007-11-15 | U S Medical Res Inst Of Infect | Hoch-durchsatz assays für proteolytische aktivitäten von clostridium neurotoxinen |
| US7332567B2 (en) | 2001-08-28 | 2008-02-19 | Allergan, Inc. | Fret protease assays for clostridial toxins |
| US7208285B2 (en) | 2001-08-28 | 2007-04-24 | Allergan, Inc. | Fret protease assays for botulinum serotype A/E toxins |
| US7374896B2 (en) | 2001-08-28 | 2008-05-20 | Allergan, Inc. | GFP-SNAP25 fluorescence release assay for botulinum neurotoxin protease activity |
| US20060063221A1 (en) * | 2004-09-21 | 2006-03-23 | Williams Dudley J | Lanthanide-based substrates and methods for determining clostridial toxin activity |
| AU2003256239A1 (en) | 2002-08-28 | 2004-03-19 | Btb Wallercode Sdn Bhd | Rainwater collection, storage and supply system |
| WO2004029567A1 (en) | 2002-09-26 | 2004-04-08 | Rensselaer Polytechnic Institute | Analytical instruments for monitoring photopolymerization |
| US7183066B2 (en) * | 2002-09-27 | 2007-02-27 | Allergan, Inc. | Cell-based fluorescence resonance energy transfer (FRET) assays for clostridial toxins |
| AU2003272800A1 (en) | 2002-10-01 | 2004-04-23 | University Of Maryland | Methods for identifying inhibitors of botulinum neurotoxins |
| US20050100983A1 (en) * | 2003-11-06 | 2005-05-12 | Coley Pharmaceutical Gmbh | Cell-free methods for identifying compounds that affect toll-like receptor 9 (TLR9) signaling |
| WO2005076785A2 (en) | 2003-12-19 | 2005-08-25 | Wisconsin Alumni Research Foundation | Method and compositions for detecting botulinum neurotoxin |
| CA2565678C (fr) | 2004-05-05 | 2013-07-09 | Pharmaleads | Substrats peptidiques reconnus par la toxine botulique de type a, bont/a et leurs utilisations |
| US7399607B2 (en) * | 2004-09-22 | 2008-07-15 | Allergan, Inc. | Fluorescence polarization assays for determining clostridial toxin activity |
| ATE552500T1 (de) * | 2005-04-05 | 2012-04-15 | Allergan Inc | Lipophile, farbstoffbasierte fret-assays für die clostridientoxinaktivität |
-
2006
- 2006-04-04 AT AT10164461T patent/ATE552500T1/de active
- 2006-04-04 AT AT06749210T patent/ATE470857T1/de active
- 2006-04-04 DE DE602006014812T patent/DE602006014812D1/de not_active Expired - Lifetime
- 2006-04-04 AU AU2006231470A patent/AU2006231470B2/en not_active Ceased
- 2006-04-04 CA CA2604039A patent/CA2604039C/en not_active Expired - Fee Related
- 2006-04-04 ES ES06749210T patent/ES2345506T3/es not_active Expired - Lifetime
- 2006-04-04 DK DK06749210.8T patent/DK1869459T3/da active
- 2006-04-04 EP EP10164461A patent/EP2293064B1/de not_active Expired - Lifetime
- 2006-04-04 EP EP06749210A patent/EP1869459B1/de not_active Expired - Lifetime
- 2006-04-04 PT PT06749210T patent/PT1869459E/pt unknown
- 2006-04-04 WO PCT/US2006/012426 patent/WO2006107921A2/en not_active Ceased
- 2006-04-04 US US11/908,438 patent/US8124357B2/en not_active Expired - Fee Related
-
2012
- 2012-02-27 US US13/406,224 patent/US8535941B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2345506T3 (es) | 2010-09-24 |
| PT1869459E (pt) | 2010-08-10 |
| US8124357B2 (en) | 2012-02-28 |
| EP1869459B1 (de) | 2010-06-09 |
| DE602006014812D1 (de) | 2010-07-22 |
| EP1869459A2 (de) | 2007-12-26 |
| ATE470857T1 (de) | 2010-06-15 |
| EP2293064B1 (de) | 2012-04-04 |
| WO2006107921A3 (en) | 2007-01-18 |
| US8535941B2 (en) | 2013-09-17 |
| DK1869459T3 (da) | 2010-09-27 |
| CA2604039C (en) | 2014-09-16 |
| AU2006231470A1 (en) | 2006-10-12 |
| WO2006107921A2 (en) | 2006-10-12 |
| US20080160561A1 (en) | 2008-07-03 |
| CA2604039A1 (en) | 2006-10-12 |
| EP2293064A1 (de) | 2011-03-09 |
| AU2006231470B2 (en) | 2011-12-01 |
| US20120231538A1 (en) | 2012-09-13 |
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