WO2009017234A1 - γ-セクレターゼの新規基質c-Retを利用したスクリーニング方法 - Google Patents

γ-セクレターゼの新規基質c-Retを利用したスクリーニング方法 Download PDF

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Publication number
WO2009017234A1
WO2009017234A1 PCT/JP2008/063901 JP2008063901W WO2009017234A1 WO 2009017234 A1 WO2009017234 A1 WO 2009017234A1 JP 2008063901 W JP2008063901 W JP 2008063901W WO 2009017234 A1 WO2009017234 A1 WO 2009017234A1
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WO
WIPO (PCT)
Prior art keywords
ret
secretase
screening method
gamma
method utilizing
Prior art date
Application number
PCT/JP2008/063901
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English (en)
French (fr)
Inventor
Eiji Inoue
Original Assignee
Eisai R & D Management Co., Ltd.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eisai R & D Management Co., Ltd. filed Critical Eisai R & D Management Co., Ltd.
Priority to US12/670,987 priority Critical patent/US20100190184A1/en
Priority to EP08792114A priority patent/EP2192181A4/en
Priority to JP2009525466A priority patent/JPWO2009017234A1/ja
Publication of WO2009017234A1 publication Critical patent/WO2009017234A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G01N2333/964—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue
    • G01N2333/96425—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals
    • G01N2333/96427—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general
    • G01N2333/9643—Proteinases, i.e. endopeptidases (3.4.21-3.4.99) derived from animal tissue from mammals in general with EC number
    • G01N2333/96472—Aspartic endopeptidases (3.4.23)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　　本発明は、γ-セクレターゼによるc-Retのプロセシングに影響を与える化合物をスクリーニングする方法であって、以下のステップ： 　(i)候補化合物の存在下および非存在下で、γ-セクレターゼまたはその生物学的に活性なその断片を含む第１の生物学的組成物と、c-Retを含む第２の生物学的組成物と接触させ； 　(ii)当該候補化合物の存在下および非存在下における当該c-Retの開裂をそれぞれ測定し； 　(iii)γ-セクレターゼによる当該c-Retの開裂に影響を与える候補化合物を選択し；そして 　(iv)前記(iii)で得られた候補化合物を、γ-セクレターゼによるc-Retのプロセシングに影響を与える化合物であると同定する、 　ことを含む、上記方法を提供する。
PCT/JP2008/063901 2007-08-01 2008-08-01 γ-セクレターゼの新規基質c-Retを利用したスクリーニング方法 WO2009017234A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/670,987 US20100190184A1 (en) 2007-08-01 2008-08-01 Screening method utilizing novel substrate c-ret for gamma-secretase
EP08792114A EP2192181A4 (en) 2007-08-01 2008-08-01 SCREENING METHOD USING THE NEW C-RET SUBSTRATE FOR GAMMA-SECRETASE
JP2009525466A JPWO2009017234A1 (ja) 2007-08-01 2008-08-01 γ−セクレターゼの新規基質ｃ−Ｒｅｔを利用したスクリーニング方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US95323007P 2007-08-01 2007-08-01
US60/953,230 2007-08-01

Publications (1)

Publication Number Publication Date
WO2009017234A1 true WO2009017234A1 (ja) 2009-02-05

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/063901 WO2009017234A1 (ja) 2007-08-01 2008-08-01 γ-セクレターゼの新規基質c-Retを利用したスクリーニング方法

Country Status (4)

Country Link
US (1) US20100190184A1 (ja)
EP (1) EP2192181A4 (ja)
JP (1) JPWO2009017234A1 (ja)
WO (1) WO2009017234A1 (ja)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPWO2009011426A1 (ja) * 2007-07-19 2010-09-24 エーザイ・アール・アンド・ディー・マネジメント株式会社 γ−セクレターゼの新規基質ｃ−Ｍｅｔを利用したスクリーニング方法
WO2009064006A1 (ja) * 2007-11-15 2009-05-22 Eisai R & D Management Co., Ltd. γ-セクレターゼの新規基質EphA7を利用したスクリーニング方法
WO2009069808A1 (ja) * 2007-11-30 2009-06-04 Eisai R & D Management Co., Ltd. 新規活性を有するEphA4ポリペプチドおよびその用途
US8865426B2 (en) 2010-12-17 2014-10-21 Eisai R&D Management Co., Ltd. Screening method using gelatinase-mediated EphA4 cleavage reaction as an indicator
JP5961608B2 (ja) 2011-04-25 2016-08-02 エーザイ・アール・アンド・ディー・マネジメント株式会社 ＥｐｈＡ４細胞外ドメインの測定による認知機能障害を伴う神経系疾患の検出方法
KR20220027825A (ko) 2019-07-01 2022-03-08 에자이 알앤드디 매니지먼트 가부시키가이샤 항-EphA4 항체

Citations (3)

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WO1998045708A1 (en) * 1997-04-08 1998-10-15 Sugen, Inc. Study and treatment of diseases related to specific cellular functions of receptor protein tyrosine kinases
WO2003016475A2 (en) * 2001-08-14 2003-02-27 The General Hospital Corporation Nucleic acid and amino acid sequences involved in pain
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US20020068361A1 (en) * 1997-04-08 2002-06-06 Douglas Clary Methods of evaluating specific cellular functions of receptor protein tyrosine kinases in a ligand independent manner
US20070026409A1 (en) * 2001-08-14 2007-02-01 The General Hospital Corporation Nucleic acid and amino acid sequences involved in pain
US20060241074A1 (en) * 2001-08-14 2006-10-26 The General Hospital Corporation Methods for treatment of pain
EP1662259A1 (en) * 2004-11-25 2006-05-31 Cellzome Ag Use of Eph receptor inhibitors for the treatment of neurodegenerative diseases
WO2008150010A1 (ja) * 2007-06-08 2008-12-11 Eisai R & D Management Co., Ltd. γ-セクレターゼの新規基質EphA4を利用したスクリーニング方法
US20090023158A1 (en) * 2007-07-13 2009-01-22 Elan Pharmaceuticals, Inc. Compositions and Methods for Identifying Substrate Specificity of Inhibitors of Gamma Secretase
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WO2003016475A2 (en) * 2001-08-14 2003-02-27 The General Hospital Corporation Nucleic acid and amino acid sequences involved in pain
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Also Published As

Publication number Publication date
JPWO2009017234A1 (ja) 2010-10-28
EP2192181A4 (en) 2010-12-08
EP2192181A1 (en) 2010-06-02
US20100190184A1 (en) 2010-07-29

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