WO2009064006A1 - γ-セクレターゼの新規基質EphA7を利用したスクリーニング方法 - Google Patents

γ-セクレターゼの新規基質EphA7を利用したスクリーニング方法 Download PDF

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Publication number
WO2009064006A1
WO2009064006A1 PCT/JP2008/070864 JP2008070864W WO2009064006A1 WO 2009064006 A1 WO2009064006 A1 WO 2009064006A1 JP 2008070864 W JP2008070864 W JP 2008070864W WO 2009064006 A1 WO2009064006 A1 WO 2009064006A1
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WIPO (PCT)
Prior art keywords
epha7
secretase
screening method
method utilizing
novel substrate
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PCT/JP2008/070864
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English (en)
French (fr)
Inventor
Eiji Inoue
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Eisai R & D Management Co., Ltd.
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Application filed by Eisai R & D Management Co., Ltd. filed Critical Eisai R & D Management Co., Ltd.
Priority to US12/742,312 priority Critical patent/US8530181B2/en
Priority to EP08849729A priority patent/EP2219028B1/en
Priority to JP2009541201A priority patent/JP5580598B2/ja
Publication of WO2009064006A1 publication Critical patent/WO2009064006A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

　本発明は、γ-セクレターゼによるEphA7のプロセシングに影響を与える化合物をスクリーニングする方法であって、以下のステップ： 　　　(i) 候補化合物の存在下および非存在下で、γ-セクレターゼまたはその生物学的に活性なその断片を含む第１の生物学的組成物を、EphA7を含む第２の生物学的組成物と接触させ； 　　　(ii) 当該候補化合物の存在下と非存在下で、当該EphA7の開裂を測定し； 　　　(iii) γ-セクレターゼによる当該EphA7の開裂に影響を与える当該候補化合物を選択し；そして 　　　(iv) 前記(iii)で得られた候補化合物が、γ-セクレターゼによるEphA7のプロセシングに影響を与える化合物であると同定する、 ことを含む、上記方法を提供する。
PCT/JP2008/070864 2007-11-15 2008-11-17 γ-セクレターゼの新規基質EphA7を利用したスクリーニング方法 WO2009064006A1 (ja)

Priority Applications (3)

Application Number Priority Date Filing Date Title
US12/742,312 US8530181B2 (en) 2007-11-15 2008-11-17 Method of screening for compounds which affect the cleavage of EphA7 byγ-secretase
EP08849729A EP2219028B1 (en) 2007-11-15 2008-11-17 Screening method utilizing novel substrate epha7 for gamma-secretase
JP2009541201A JP5580598B2 (ja) 2007-11-15 2008-11-17 γ−セクレターゼの新規基質ＥｐｈＡ７を利用したスクリーニング方法

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US98820307P 2007-11-15 2007-11-15
US60/988,203 2007-11-15

Publications (1)

Publication Number Publication Date
WO2009064006A1 true WO2009064006A1 (ja) 2009-05-22

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Country Link
US (1) US8530181B2 (ja)
EP (1) EP2219028B1 (ja)
JP (1) JP5580598B2 (ja)
WO (1) WO2009064006A1 (ja)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090191580A1 (en) * 2007-07-19 2009-07-30 Eisai R & D Management Co., Ltd. METHOD OF SCREENING USING c-MET, A NOVEL SUBSTRATE FOR GAMMA-SECRETASE
US20100190184A1 (en) * 2007-08-01 2010-07-29 Eisai R & D Management Co., Ltd. Screening method utilizing novel substrate c-ret for gamma-secretase
US8530181B2 (en) 2007-11-15 2013-09-10 Eisai R&D Management Co., Ltd. Method of screening for compounds which affect the cleavage of EphA7 byγ-secretase
EP2223999B1 (en) * 2007-11-30 2017-10-18 Eisai R&D Management Co., Ltd. Epha4 polypeptide having novel activity and use thereof
US8865426B2 (en) 2010-12-17 2014-10-21 Eisai R&D Management Co., Ltd. Screening method using gelatinase-mediated EphA4 cleavage reaction as an indicator
US9784751B2 (en) 2011-04-25 2017-10-10 Eisai R&D Management Co., Ltd. Method for detecting neurological disease associated with cognitive impairment by measuring EphA4 extracellular domain
MX2021015298A (es) 2019-07-01 2022-01-18 Eisai R&D Man Co Ltd Anticuerpo anti-epha4 humano.

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Also Published As

Publication number Publication date
US20100255522A1 (en) 2010-10-07
JPWO2009064006A1 (ja) 2011-03-31
EP2219028A1 (en) 2010-08-18
EP2219028B1 (en) 2012-09-12
EP2219028A4 (en) 2010-12-29
US8530181B2 (en) 2013-09-10
JP5580598B2 (ja) 2014-08-27

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