WO2009011426A1 - γ-セクレターゼの新規基質c-Metを利用したスクリーニング方法 - Google Patents

γ-セクレターゼの新規基質c-Metを利用したスクリーニング方法 Download PDF

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Publication number
WO2009011426A1
WO2009011426A1 PCT/JP2008/063037 JP2008063037W WO2009011426A1 WO 2009011426 A1 WO2009011426 A1 WO 2009011426A1 JP 2008063037 W JP2008063037 W JP 2008063037W WO 2009011426 A1 WO2009011426 A1 WO 2009011426A1
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Prior art keywords
met
secretase
screening method
method utilizing
novel substrate
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PCT/JP2008/063037
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English (en)
French (fr)
Inventor
Eiji Inoue
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Eisai R & D Management Co., Ltd.
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Publication date
Application filed by Eisai R & D Management Co., Ltd. filed Critical Eisai R & D Management Co., Ltd.
Priority to JP2009523688A priority Critical patent/JPWO2009011426A1/ja
Priority to EP08791346A priority patent/EP2177623A4/en
Publication of WO2009011426A1 publication Critical patent/WO2009011426A1/ja

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435—Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/705—Assays involving receptors, cell surface antigens or cell surface determinants
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/948—Hydrolases (3) acting on peptide bonds (3.4)
    • G01N2333/95—Proteinases, i.e. endopeptidases (3.4.21-3.4.99)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/02—Screening involving studying the effect of compounds C on the interaction between interacting molecules A and B (e.g. A = enzyme and B = substrate for A, or A = receptor and B = ligand for the receptor)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Endocrinology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

　本発明は、γ-セクレターゼによるc-Metのプロセシングに影響を与える化合物をスクリーニングする方法であって、以下のステップ： 　　(i)候補化合物の存在下および非存在下で、γ-セクレターゼまたはその生物学的に活性な断片を含む第１の生物学的組成物と、c-Metを含む第２の生物学的組成物とを接触させ； 　　(ii)当該候補化合物の存在下および非存在下における当該c-Metの開裂をそれぞれ測定し； 　　(iii)γ-セクレターゼによる当該c-Metの開裂に影響を与える候補化合物を選択し；そして 　　(iv)前記(iii)で得られた候補化合物を、γ-セクレターゼによるc-Metのプロセシングに影響を与える化合物であると同定する、 ことを含む、上記方法。
PCT/JP2008/063037 2007-07-19 2008-07-18 γ-セクレターゼの新規基質c-Metを利用したスクリーニング方法 WO2009011426A1 (ja)

Priority Applications (2)

Application Number Priority Date Filing Date Title
JP2009523688A JPWO2009011426A1 (ja) 2007-07-19 2008-07-18 γ−セクレターゼの新規基質ｃ−Ｍｅｔを利用したスクリーニング方法
EP08791346A EP2177623A4 (en) 2007-07-19 2008-07-18 SCREENING METHOD USING C-MET, A NEW SUBSTRATE FOR GAMMA SECRETASE

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US95062107P 2007-07-19 2007-07-19
US60/950,621 2007-07-19
US95149307P 2007-07-24 2007-07-24
US60/951,493 2007-07-24

Publications (1)

Publication Number Publication Date
WO2009011426A1 true WO2009011426A1 (ja) 2009-01-22

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Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2008/063037 WO2009011426A1 (ja) 2007-07-19 2008-07-18 γ-セクレターゼの新規基質c-Metを利用したスクリーニング方法

Country Status (4)

Country Link
US (1) US20090191580A1 (ja)
EP (1) EP2177623A4 (ja)
JP (1) JPWO2009011426A1 (ja)
WO (1) WO2009011426A1 (ja)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008150010A1 (ja) * 2007-06-08 2008-12-11 Eisai R & D Management Co., Ltd. γ-セクレターゼの新規基質EphA4を利用したスクリーニング方法
EP2192181A4 (en) * 2007-08-01 2010-12-08 Eisai R&D Man Co Ltd SCREENING METHOD USING THE NEW C-RET SUBSTRATE FOR GAMMA-SECRETASE
JP5580598B2 (ja) * 2007-11-15 2014-08-27 エーザイ・アール・アンド・ディー・マネジメント株式会社 γ−セクレターゼの新規基質ＥｐｈＡ７を利用したスクリーニング方法
WO2009069808A1 (ja) * 2007-11-30 2009-06-04 Eisai R & D Management Co., Ltd. 新規活性を有するEphA4ポリペプチドおよびその用途
WO2012059562A1 (en) 2010-11-03 2012-05-10 Argen-X-Bv C-met antibody combinations
US8865426B2 (en) 2010-12-17 2014-10-21 Eisai R&D Management Co., Ltd. Screening method using gelatinase-mediated EphA4 cleavage reaction as an indicator
EP2703814B1 (en) 2011-04-25 2017-11-01 Eisai R&D Management Co., Ltd. Method for detecting neurological disease associated with cognitive dysfunction by measuring epha4 extracellular domain
MA56466A (fr) 2019-07-01 2022-05-11 Eisai R&D Man Co Ltd Anticorps anti-epha4

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US20020068361A1 (en) * 1997-04-08 2002-06-06 Douglas Clary Methods of evaluating specific cellular functions of receptor protein tyrosine kinases in a ligand independent manner
US20060241074A1 (en) * 2001-08-14 2006-10-26 The General Hospital Corporation Methods for treatment of pain
US20070026409A1 (en) * 2001-08-14 2007-02-01 The General Hospital Corporation Nucleic acid and amino acid sequences involved in pain
EP1478772A2 (en) * 2001-08-14 2004-11-24 The General Hospital Corporation Nucleic acid and amino acid sequences involved in pain
JP2004000060A (ja) * 2002-05-31 2004-01-08 Otsuka Pharmaceut Co Ltd アミロイドβ産生に影響を与える化合物のスクリーニング方法
EP1662259A1 (en) * 2004-11-25 2006-05-31 Cellzome Ag Use of Eph receptor inhibitors for the treatment of neurodegenerative diseases
EP1947193A1 (en) * 2007-01-17 2008-07-23 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Screening method for anti-diabetic compounds
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Publication number Publication date
EP2177623A4 (en) 2010-12-29
EP2177623A1 (en) 2010-04-21
JPWO2009011426A1 (ja) 2010-09-24
US20090191580A1 (en) 2009-07-30

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