ATE470707T1 - Zytotoxizitätstest - Google Patents
ZytotoxizitätstestInfo
- Publication number
- ATE470707T1 ATE470707T1 AT03719654T AT03719654T ATE470707T1 AT E470707 T1 ATE470707 T1 AT E470707T1 AT 03719654 T AT03719654 T AT 03719654T AT 03719654 T AT03719654 T AT 03719654T AT E470707 T1 ATE470707 T1 AT E470707T1
- Authority
- AT
- Austria
- Prior art keywords
- dye
- cells
- production
- reduced state
- reagent mixture
- Prior art date
Links
- 231100000263 cytotoxicity test Toxicity 0.000 title 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 239000000203 mixture Substances 0.000 abstract 3
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 239000003795 chemical substances by application Substances 0.000 abstract 2
- 230000001086 cytosolic effect Effects 0.000 abstract 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 239000000758 substrate Substances 0.000 abstract 2
- 102000003855 L-lactate dehydrogenase Human genes 0.000 abstract 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 abstract 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 abstract 1
- 231100000135 cytotoxicity Toxicity 0.000 abstract 1
- 230000003013 cytotoxicity Effects 0.000 abstract 1
- 239000012992 electron transfer agent Substances 0.000 abstract 1
- 231100000252 nontoxic Toxicity 0.000 abstract 1
- 230000003000 nontoxic effect Effects 0.000 abstract 1
- 239000002904 solvent Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Hematology (AREA)
- Toxicology (AREA)
- Chemical & Material Sciences (AREA)
- Urology & Nephrology (AREA)
- Molecular Biology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/124,029 US6982152B2 (en) | 2002-04-17 | 2002-04-17 | Cytotoxicity assay |
| PCT/US2003/010872 WO2003089635A1 (en) | 2002-04-17 | 2003-04-09 | Cytotoxicity assay |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE470707T1 true ATE470707T1 (de) | 2010-06-15 |
Family
ID=29248365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT03719654T ATE470707T1 (de) | 2002-04-17 | 2003-04-09 | Zytotoxizitätstest |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US6982152B2 (de) |
| EP (1) | EP1499717B1 (de) |
| JP (1) | JP4544867B2 (de) |
| AT (1) | ATE470707T1 (de) |
| CA (1) | CA2481548A1 (de) |
| DE (1) | DE60332934D1 (de) |
| ES (1) | ES2347232T3 (de) |
| WO (1) | WO2003089635A1 (de) |
Families Citing this family (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6982152B2 (en) * | 2002-04-17 | 2006-01-03 | Promega Corporation | Cytotoxicity assay |
| EP2177622B1 (de) | 2005-12-12 | 2013-01-16 | Roche Diagnostics GmbH | Chromogene Tests in Gegenwart von Zellen |
| MX2008013002A (es) | 2006-04-10 | 2008-10-17 | Wisconsin Alumni Res Found | Reactivos y metodos para usar celulas madre embrionarias humanas para evaluar toxicidad de compuestos farmaceuticos y otros quimicos. |
| WO2008040532A1 (en) * | 2006-10-02 | 2008-04-10 | Cellartis Ab | Novel toxicity assay based on human blastocyst-derived stem cells and progenitor cells |
| EP2215472B1 (de) * | 2007-11-09 | 2014-06-18 | Genzyme Corporation | Verfahren zur messung der lebensfähigkeit von zellen ohne verwendung von kontrollzellen |
| SE534542C2 (sv) * | 2009-09-30 | 2011-09-27 | Calmark Sweden Ab | Testsystem för bestämning av hypoxiutlöst cellskada |
| US20130210063A1 (en) * | 2010-10-13 | 2013-08-15 | Life Technologies Corporation | Compositions and assays for determining cell viability |
| DE102011007769B3 (de) * | 2011-04-20 | 2012-06-21 | Siemens Aktiengesellschaft | Verfahren und Vorrichtung zur Untersuchung von Tabakrauch |
| DE102011078184B3 (de) * | 2011-06-28 | 2012-10-25 | Siemens Aktiengesellschaft | Biotransformationssensor |
| ES2527796B1 (es) * | 2013-07-29 | 2015-11-10 | Universidad De Almería | Método para la cuantificación fluorimétrica de la enzina LDH en disolución |
| KR101880206B1 (ko) | 2014-04-18 | 2018-07-20 | 도진도 래보라토리즈 | 탈수소 효소 및 이의 기질 농도 측정 방법, 전자 메디에이터 및 상기 전자 메디에이터를 포함하는 탈수소 효소 및 이의 기질용 측정 시약 |
| US11378570B2 (en) | 2017-05-24 | 2022-07-05 | Alcon Inc. | Cytotoxicity test method for medical devices |
| JP7394367B2 (ja) * | 2018-06-28 | 2023-12-08 | ダイキン工業株式会社 | 気体の細胞毒性評価方法 |
| WO2020014153A1 (en) * | 2018-07-09 | 2020-01-16 | The Regents Of The University Of California | Redox-labile fluorescent probes and their surface immobilization methods for the detection of metabolites |
| US12000819B2 (en) | 2018-07-25 | 2024-06-04 | Daikin Industries, Ltd. | Gas concentration prediction method |
| US11590502B2 (en) | 2019-07-01 | 2023-02-28 | General Electric Company | Assessment of micro-organism presence |
| US11597960B2 (en) | 2019-07-01 | 2023-03-07 | General Electric Company | Assessment of micro-organism presence |
| KR20230044440A (ko) * | 2020-07-24 | 2023-04-04 | 더 유니버시티 오브 털사 | N-벤질 설폰아미드의 합성 및 사용 |
| CA3250237A1 (en) * | 2022-01-26 | 2023-08-03 | The University Of Tulsa | COMPOUND SCREENING |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3107204A (en) | 1961-01-23 | 1963-10-15 | Dalde Reagents Inc | Microbiological testing method and structure therefor |
| US3384555A (en) * | 1965-11-30 | 1968-05-21 | Army Usa | Direct fluorometric method for measuring dehydrogenase activity |
| GB1535643A (en) | 1976-10-26 | 1978-12-13 | Ayerst Mckenna & Harrison | Determination of the susceptibility of bacteria to antibacterial agents |
| US4849347A (en) | 1984-11-29 | 1989-07-18 | Hoffmann-La Roche Inc. | Colorimetric biological assay |
| JPS6431379A (en) | 1987-07-27 | 1989-02-01 | Matsushita Electric Industrial Co Ltd | High-frequency heating device |
| DE3826922A1 (de) * | 1988-08-09 | 1990-02-22 | Boehringer Mannheim Gmbh | Verfahren zur kolorimetrischen bestimmung eines analyten mittels enzymatischer oxidation |
| US5501959A (en) | 1989-01-17 | 1996-03-26 | Alamar Biosciences Laboratory, Inc. | Antibiotic and cytotoxic drug susceptibility assays using resazurin and poising agents |
| JPH02211899A (ja) | 1989-02-10 | 1990-08-23 | Terumo Corp | 細菌の薬剤感受性測定方法 |
| US5185450A (en) | 1991-02-19 | 1993-02-09 | University Of South Florida | Tetrazolium compounds for cell viability assays |
| JP3203268B2 (ja) * | 1991-07-12 | 2001-08-27 | デイド・ベーリング・マルブルク・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング | カンジダ感染の検出方法 |
| US5756527A (en) | 1995-06-07 | 1998-05-26 | Ontogen Corporation | Imidazole derivatives useful as modulators of multi drug resistances |
| EP0839156A1 (de) | 1995-07-20 | 1998-05-06 | Xoma Corporation | Peptide gegen pilzbefall |
| JPH1033196A (ja) | 1996-07-23 | 1998-02-10 | Unitika Ltd | 試験片 |
| DE19639169A1 (de) * | 1996-09-24 | 1998-04-02 | Boehringer Mannheim Gmbh | Redoxaktive Verbindungen und deren Anwendung |
| DE69937835T2 (de) * | 1998-09-24 | 2009-01-02 | Promega Corp., Madison | Antikörper für apoptosemarker und anwendungsverfahren |
| WO2001098531A1 (en) | 2000-06-20 | 2001-12-27 | Ortho-Mcneil Pharmaceutical, Inc. | Resazurin-based cytotoxicity assay |
| US6811990B1 (en) | 2001-02-13 | 2004-11-02 | Michael J. Corey | Methods and compositions for coupled luminescent assays |
| US6982152B2 (en) * | 2002-04-17 | 2006-01-03 | Promega Corporation | Cytotoxicity assay |
| US20030232401A1 (en) * | 2002-06-12 | 2003-12-18 | Pugia Michael J. | Bacterial test method by glycated label binding |
-
2002
- 2002-04-17 US US10/124,029 patent/US6982152B2/en not_active Expired - Lifetime
-
2003
- 2003-04-09 AT AT03719654T patent/ATE470707T1/de active
- 2003-04-09 ES ES03719654T patent/ES2347232T3/es not_active Expired - Lifetime
- 2003-04-09 DE DE60332934T patent/DE60332934D1/de not_active Expired - Lifetime
- 2003-04-09 CA CA002481548A patent/CA2481548A1/en not_active Abandoned
- 2003-04-09 WO PCT/US2003/010872 patent/WO2003089635A1/en not_active Ceased
- 2003-04-09 JP JP2003586347A patent/JP4544867B2/ja not_active Expired - Fee Related
- 2003-04-09 EP EP03719654A patent/EP1499717B1/de not_active Expired - Lifetime
-
2005
- 2005-03-24 US US11/087,777 patent/US7282348B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| AU2003223521A1 (en) | 2003-11-03 |
| JP4544867B2 (ja) | 2010-09-15 |
| EP1499717A4 (de) | 2007-01-03 |
| EP1499717A1 (de) | 2005-01-26 |
| CA2481548A1 (en) | 2003-10-30 |
| US20050186557A1 (en) | 2005-08-25 |
| US7282348B2 (en) | 2007-10-16 |
| JP2005523022A (ja) | 2005-08-04 |
| WO2003089635A1 (en) | 2003-10-30 |
| DE60332934D1 (de) | 2010-07-22 |
| ES2347232T3 (es) | 2010-10-27 |
| US6982152B2 (en) | 2006-01-03 |
| EP1499717B1 (de) | 2010-06-09 |
| US20030203420A1 (en) | 2003-10-30 |
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| Date | Code | Title | Description |
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| UEP | Publication of translation of european patent specification |
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