ATE377756T1 - Verfahren und gerät für die identifikation und zuordnung von biomolekülen - Google Patents

Verfahren und gerät für die identifikation und zuordnung von biomolekülen

Info

Publication number
ATE377756T1
ATE377756T1 AT03713891T AT03713891T ATE377756T1 AT E377756 T1 ATE377756 T1 AT E377756T1 AT 03713891 T AT03713891 T AT 03713891T AT 03713891 T AT03713891 T AT 03713891T AT E377756 T1 ATE377756 T1 AT E377756T1
Authority
AT
Austria
Prior art keywords
membrane
digestion
electroblotted
polypeptides
polypeptide
Prior art date
Application number
AT03713891T
Other languages
English (en)
Inventor
Timothy Nadler
Kenneth Parker
George Vella
Barrie Wagenfeld
Yulin Huang
Robert Lotti
Original Assignee
Perseptive Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Perseptive Biosystems Inc filed Critical Perseptive Biosystems Inc
Application granted granted Critical
Publication of ATE377756T1 publication Critical patent/ATE377756T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6848—Methods of protein analysis involving mass spectrometry
    • G01N33/6851—Methods of protein analysis involving laser desorption ionisation mass spectrometry

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • Optics & Photonics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Discharge Of Articles From Conveyors (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AT03713891T 2002-03-12 2003-03-04 Verfahren und gerät für die identifikation und zuordnung von biomolekülen ATE377756T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US36366302P 2002-03-12 2002-03-12
US10/327,342 US7166441B2 (en) 2002-03-12 2002-12-20 Method and apparatus for the identification and quantification of biomolecules

Publications (1)

Publication Number Publication Date
ATE377756T1 true ATE377756T1 (de) 2007-11-15

Family

ID=28044867

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03713891T ATE377756T1 (de) 2002-03-12 2003-03-04 Verfahren und gerät für die identifikation und zuordnung von biomolekülen

Country Status (6)

Country Link
US (1) US7166441B2 (de)
EP (1) EP1490394B1 (de)
JP (1) JP4291161B2 (de)
AT (1) ATE377756T1 (de)
DE (1) DE60317314T2 (de)
WO (1) WO2003078452A1 (de)

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CA2243230A1 (en) * 1998-07-15 2000-01-15 Aled Edwards A device and method for the determination of protein domain boundaries
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US7244702B2 (en) * 2003-04-23 2007-07-17 Nzedegwu Robert Olisa, III Purified Cys moiety for iron reductase, and method for obtaining and using same
JP2007508552A (ja) * 2003-10-10 2007-04-05 プロテイン・デイスカバリー・インコーポレーテツド マトリックス支援レーザー脱離／イオン化（ｍａｌｄｉ）質量分析法（ｍｓ）を包含する化学分析のための被検体の濃縮および精製のための方法および装置
US7227066B1 (en) * 2004-04-21 2007-06-05 Nanosolar, Inc. Polycrystalline optoelectronic devices based on templating technique
JP4317083B2 (ja) * 2004-06-04 2009-08-19 株式会社日立ハイテクノロジーズ 質量分析方法及び質量分析システム
JP2006078192A (ja) * 2004-09-07 2006-03-23 Shimadzu Corp 生体高分子の質量分析法
WO2006055615A2 (en) * 2004-11-15 2006-05-26 University Of North Dakota A method for single oxygen atom incorporation into digested peptides using peptidases
AU2006235320A1 (en) * 2005-04-05 2006-10-19 Protein Discovery, Inc. Improved methods and devices for concentration and fractionation of analytes for chemical analysis including Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry (MS)
US20070187244A1 (en) * 2005-12-13 2007-08-16 Bio-Rad Laboratories, Inc. Composite Membrane To Capture Analyte Transfers From Gels
JP2007309673A (ja) * 2006-05-16 2007-11-29 Shionogi & Co Ltd 微量タンパク質の同定を可能にするｍａｌｄｉ型質量分析装置用サンプルプレート
US8580534B2 (en) * 2006-06-30 2013-11-12 The University Of North Dakota Method for incorporation of two oxygen atoms into digested peptides using peptidases
US20090071834A1 (en) * 2007-06-08 2009-03-19 Protein Discovery, Inc. Methods and Devices for Concentration and Fractionation of Analytes for Chemical Analysis Including Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometry (MS)
US8075755B2 (en) * 2007-12-13 2011-12-13 Bio-Rad Laboratories, Inc. Polymeric sorbent sheets as ion reservoirs for electroblotting
TW201014605A (en) 2008-09-16 2010-04-16 Genentech Inc Methods for treating progressive multiple sclerosis
US20100213060A1 (en) * 2009-02-23 2010-08-26 Bio-Rad Laboratories, Inc. Aqueous transfer buffer
CN102439398A (zh) 2009-04-27 2012-05-02 蛋白质发现公司 可编程电泳凹口过滤器系统及方法
US8325999B2 (en) 2009-06-08 2012-12-04 Microsoft Corporation Assisted face recognition tagging
US8715476B2 (en) 2009-12-11 2014-05-06 Bio-Rad Laboratories, Inc. Instrument for independent electrotransfer in multiple cassettes
CN101793865A (zh) * 2010-03-29 2010-08-04 内蒙古蒙牛乳业（集团）股份有限公司 一种检测人乳中人血清白蛋白含量的方法
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
WO2013147610A2 (en) 2012-03-30 2013-10-03 Jetze Botma Automated selection of microorganisms and identification using maldi
DE102012011647B4 (de) * 2012-06-08 2020-07-02 Bruker Daltonik Gmbh Analyse von Mikroben aus Mikrokolonien mittels MALDI-Massenspektrometrie
DE102012011648B4 (de) 2012-06-08 2018-06-14 Bruker Daltonik Gmbh Analyse von Mikroben aus Mikrokolonien mittels MALDI-Massenspektrometrie
CN103969321B (zh) * 2013-01-24 2016-04-20 复旦大学 基于固定化酶原位高效酶解法的蛋白鉴定及质谱成像的方法
US9879313B2 (en) 2013-06-25 2018-01-30 Prognosys Biosciences, Inc. Methods and systems for determining spatial patterns of biological targets in a sample
TWI518316B (zh) * 2014-04-01 2016-01-21 財團法人工業技術研究院 光學測讀系統及使用該系統之生化檢測方法
WO2016143223A1 (ja) * 2015-03-09 2016-09-15 株式会社島津製作所 外部環境変化に対する耐性が向上した固定化プロテアーゼ
CN113186256A (zh) 2015-04-10 2021-07-30 空间转录公司 生物样本的空间区别、多重核酸分析
US10996227B2 (en) * 2015-06-09 2021-05-04 Vanderbilt University Pre-coated surfaces for imaging biomolecules
CN105547967B (zh) * 2016-01-28 2019-04-02 成都理工大学 裂隙介质系统渗透张量室内测定装置
US20210387175A1 (en) * 2018-04-11 2021-12-16 The Trustees Of Indiana University Cartridges, systems and methods for mass spectrometry
EP3894590A2 (de) 2018-12-10 2021-10-20 10X Genomics, Inc. Verfahren zur verwendung von master/kopie-anordnungen zur räumlichen detektion
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11768175B1 (en) * 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
CN111929132B (zh) * 2020-08-07 2023-05-02 中央民族大学 一种交变电场耦合高频振动辅助基质喷涂方法
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes

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US5625184A (en) * 1995-05-19 1997-04-29 Perseptive Biosystems, Inc. Time-of-flight mass spectrometry analysis of biomolecules
US5808300A (en) * 1996-05-10 1998-09-15 Board Of Regents, The University Of Texas System Method and apparatus for imaging biological samples with MALDI MS
US6348688B1 (en) * 1998-02-06 2002-02-19 Perseptive Biosystems Tandem time-of-flight mass spectrometer with delayed extraction and method for use
US6221626B1 (en) * 1998-06-30 2001-04-24 University Of Geneva Kit for electroblotting polypeptides separated on an electrophoresis gel
US6441140B1 (en) * 1998-09-04 2002-08-27 Cell Signaling Technology, Inc. Production of motif-specific and context-independent antibodies using peptide libraries as antigens
AU766334B2 (en) 1999-01-28 2003-10-16 Universite De Geneve Method and kit for identifying or characterising polypeptides
US20020012920A1 (en) * 2000-11-20 2002-01-31 Kevin Gardner Method and kit for proteomic identification
US7045296B2 (en) * 2001-05-08 2006-05-16 Applera Corporation Process for analyzing protein samples

Also Published As

Publication number Publication date
JP4291161B2 (ja) 2009-07-08
DE60317314T2 (de) 2008-09-04
WO2003078452A1 (en) 2003-09-25
US20030175844A1 (en) 2003-09-18
EP1490394A4 (de) 2006-05-17
EP1490394A1 (de) 2004-12-29
EP1490394B1 (de) 2007-11-07
JP2005534284A (ja) 2005-11-17
US7166441B2 (en) 2007-01-23
DE60317314D1 (de) 2007-12-20

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