ATE34770T1 - Dna-vektoren und ihre verwendung in der rekombinanten dna-technologie. - Google Patents

Dna-vektoren und ihre verwendung in der rekombinanten dna-technologie.

Info

Publication number
ATE34770T1
ATE34770T1 AT84301997T AT84301997T ATE34770T1 AT E34770 T1 ATE34770 T1 AT E34770T1 AT 84301997 T AT84301997 T AT 84301997T AT 84301997 T AT84301997 T AT 84301997T AT E34770 T1 ATE34770 T1 AT E34770T1
Authority
AT
Austria
Prior art keywords
replication
origin
copy number
promoter
takes place
Prior art date
Application number
AT84301997T
Other languages
English (en)
Inventor
Geoffrey Thomas Yarranton
Gwynfor Owen Humphreys
Martyn Kim Robinson
Celia Ann Caulcott
Edwina Mary Wright
Original Assignee
Celltech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celltech Ltd filed Critical Celltech Ltd
Application granted granted Critical
Publication of ATE34770T1 publication Critical patent/ATE34770T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12N9/1033—Chloramphenicol O-acetyltransferase (2.3.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Enzymes And Modification Thereof (AREA)
AT84301997T 1983-03-25 1984-03-23 Dna-vektoren und ihre verwendung in der rekombinanten dna-technologie. ATE34770T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB838308236A GB8308236D0 (en) 1983-03-25 1983-03-25 Dna vectors
EP84301997A EP0121386B1 (de) 1983-03-25 1984-03-23 DNA-Vektoren und ihre Verwendung in der rekombinanten DNA-Technologie

Publications (1)

Publication Number Publication Date
ATE34770T1 true ATE34770T1 (de) 1988-06-15

Family

ID=10540205

Family Applications (1)

Application Number Title Priority Date Filing Date
AT84301997T ATE34770T1 (de) 1983-03-25 1984-03-23 Dna-vektoren und ihre verwendung in der rekombinanten dna-technologie.

Country Status (8)

Country Link
US (1) US5015573A (de)
EP (1) EP0121386B1 (de)
JP (2) JPH0634721B2 (de)
AT (1) ATE34770T1 (de)
AU (1) AU565352B2 (de)
DE (2) DE3471688D1 (de)
GB (2) GB8308236D0 (de)
WO (1) WO1984003713A1 (de)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
HU201116B (en) * 1982-09-16 1990-09-28 Benzon As Alfred Process for producing plasmids having uncontrolled replication conduct under given circumstances
US4710473A (en) * 1983-08-10 1987-12-01 Amgen, Inc. DNA plasmids
HU194307B (en) * 1983-09-16 1988-01-28 Richter Gedeon Vegyeszet Process for producing plasmidvectors of high copy number
WO1988006186A2 (en) * 1987-02-19 1988-08-25 The Upjohn Company cDNAs ENCODING SOMATOTROPIN, EXPRESSION VECTORS AND HOSTS
US5240837A (en) * 1987-02-19 1993-08-31 The Upjohn Company CDNAS encoding somatotropin, expression vectors and hosts
US5084384A (en) * 1987-04-23 1992-01-28 Monsanto Company Secretion of insulin-like growth factor-I
US4966841A (en) * 1987-05-22 1990-10-30 The Board Of Regents Of The University Of Washington Enhanced vector production and expression of recombinant DNA products
JPH02501533A (ja) * 1987-09-09 1990-05-31 セルテック　リミテッド ポリペプチド製造
GB8819135D0 (en) * 1988-08-11 1988-09-14 Celltech Ltd Tryptophan production
FI88309C (fi) 1989-04-20 1993-04-26 Bio Orbit Oy Ny biotest
GB9215550D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
GB9215540D0 (en) * 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
GB9215541D0 (en) 1992-07-22 1992-09-02 Celltech Ltd Protein expression system
GB9518395D0 (en) * 1995-09-08 1995-11-08 Therexsys Ltd Plasmid stabilization
US6743780B1 (en) 1995-09-08 2004-06-01 Cobra Biologics Limited Plasmid stabilization
CN1131889C (zh) 1996-03-13 2003-12-24 宝酒造株式会社 质粒
US6747129B1 (en) * 1998-09-15 2004-06-08 The Regents Of The University Of California Target of RNAIII activating protein(TRAP)
AU1031900A (en) * 1998-11-09 2000-05-29 Genecare Development Aps Novel plasmids for use in medicine and method of producing same
US6780405B1 (en) * 2000-04-28 2004-08-24 Avant Immunotherapeutics, Inc. Regulated antigen delivery system (RADS)
US7575924B2 (en) 2000-11-13 2009-08-18 Research Development Foundation Methods and compositions relating to improved lentiviral vectors and their applications
US20040137003A1 (en) * 2001-04-30 2004-07-15 Curtiss Iii Roy Regulated antigen delivery system (rads)
WO2003012054A2 (en) * 2001-08-02 2003-02-13 Institut Clayton De La Recherche Methods and compositions relating to improved lentiviral vector production systems
CA2462628C (en) 2001-10-02 2019-08-20 Institut Clayton De La Recherche Restricted expression lentiviral vectors
DK1563069T3 (da) * 2002-11-22 2012-07-23 Inst Clayton De La Rech Sammensætninger og systemer til genregulering
JP4463198B2 (ja) * 2003-03-05 2010-05-12 財団法人化学及血清療法研究所 大腸菌における異種蛋白質の製造方法
US7794971B1 (en) 2003-07-01 2010-09-14 Epicentre Technologies Corporation Compositions and methods for controlling copy number for a broad range of plasmids and uses thereof
KR100760596B1 (ko) * 2003-09-11 2007-09-20 가부시키가이샤 아드반테스트 오차 보정 신호 생성 장치 및 이 오차 보정 신호 생성장치를 구비한 직교 변조 장치
JP4972412B2 (ja) 2004-02-04 2012-07-11 メルク・シャープ・エンド・ドーム・コーポレイション Ｅ．コリ発酵によるプラスミドｄｎａの大規模生産のための方法
US7482148B2 (en) * 2004-03-30 2009-01-27 Sudershan Biotech Ltd. Recombinant calf-chymosin and a process for producing the same
WO2006081529A2 (en) 2005-01-28 2006-08-03 Nature Technology Corp. Compositions and processes for improved plasmid dna production
US8916376B2 (en) * 2007-05-15 2014-12-23 Biotex, Inc. Metal-binding peptides
AU2013373679A1 (en) 2013-01-16 2015-08-13 Inserm (Institut National De La Sante Et De La Recherche Medicale) A soluble fibroblast growth factor receptor 3 (FGR3) polypeptide for use in the prevention or treatment of skeletal growth retardation disorders
UY35368A (es) * 2013-03-08 2014-10-31 Irm Llc Péptidos y composiciones para el tratamiento de daño articular
MA38369B1 (fr) 2013-03-08 2018-10-31 Novartis Ag Peptides et compositions pour le traitement d'une lesion de l'articulation
WO2016049000A2 (en) * 2014-09-23 2016-03-31 Regeneron Pharmaceuticals, Inc. Anti-il-25 antibodies and uses thereof
PT3242888T (pt) * 2015-01-07 2020-12-28 Pfizer Armadilhas de fgfr3 solúveis para o tratamento de distúrbios do crescimento ósseo
HUE058734T2 (hu) 2016-07-07 2022-09-28 Pfizer Szolúbilis 3-as fibroblaszt növekedési faktor receptor (sFGFR3) polipeptidek és alkalmazásaik
KR102824112B1 (ko) * 2018-03-28 2025-06-24 브리스톨-마이어스 스큅 컴퍼니 인터루킨-2/인터루킨-2 수용체 알파 융합 단백질 및 사용 방법
AR116566A1 (es) 2018-10-03 2021-05-19 Novartis Ag Administración sostenida de polipéptidos similares a la angiopoyetina 3
AU2021283934A1 (en) * 2020-06-05 2023-01-19 Modernatx, Inc. Bacterial strains for DNA production

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2759053A1 (de) * 1977-12-30 1979-07-12 Uhlin Verfahren zum herstellen von genprodukten von plasmid-dns
CA1198067A (en) * 1981-02-27 1985-12-17 David H. Gelfand Stable high copy number plasmids
CA1198069A (en) * 1982-06-08 1985-12-17 David H. Gelfand Temperature sensitive multicopy plasmids
US4806471A (en) * 1982-09-16 1989-02-21 A/S Alfred Benzon Plasmids with conditional uncontrolled replication behavior

Also Published As

Publication number Publication date
AU2812484A (en) 1984-10-09
DE121386T1 (de) 1985-05-23
GB8308236D0 (en) 1983-05-05
GB2136814A (en) 1984-09-26
AU565352B2 (en) 1987-09-10
EP0121386B1 (de) 1988-06-01
JPH07114703B2 (ja) 1995-12-13
JPS60501837A (ja) 1985-10-31
EP0121386A1 (de) 1984-10-10
JPH06315388A (ja) 1994-11-15
DE3471688D1 (en) 1988-07-07
GB8407572D0 (en) 1984-05-02
WO1984003713A1 (en) 1984-09-27
JPH0634721B2 (ja) 1994-05-11
GB2136814B (en) 1987-05-28
US5015573A (en) 1991-05-14

Similar Documents

Publication Publication Date Title
DE3471688D1 (en) Dna vectors and their use in recombinant dna technology
MX9606476A (es) Inhibicion citoplasmica de expresion de genes.
EP0260148A3 (de) Rekombinantes Expressionsverfahren, Vektor und tranformierte Zellen
ATE64416T1 (de) Die verwendung des gal 1 hefe-promotors.
AU2975699A (en) Methods for producing a polypeptide in a bacillus cell
DK0770136T3 (da) Fremgangsmåde til at selektere höjtudtrykkende værtsceller
NO950534D0 (no) Modufiserte soppceller og fremgangsmåter for fremstilling av rekombinante produkter
CL2009002108A1 (es) Secuencia de adn que codifica una de secuencia aminoacidos con actividad farnesil pirofosfato sintetasa; vector que comprende dicha secuencia; celula huesped transformada con dicha secuencia; proceso de produccion de enzima para convertir isopentil pirofosfato a farnesil pirofosfato; y proceso para producir astaxantina (div. sol. no 891-99).
DK0864645T3 (da) Negativstrenget RNA-virusvektor med autonom replikeringsaktivitet
SE8603145D0 (sv) Forfarande for framstellning av interferon-beta
ES2013324A6 (es) Un metodo mejorado para producir una proteina heterologa.
ATE284954T1 (de) Hyaluronan synthase gen und seine verwendung
ATE217319T1 (de) Verfahren zur erhöhung der expression und zur verminderung der expressionsvariabilität von fremdgenen in pflanzenzellen
DE69638196D1 (de) Rekombinantes sendai-virus
SG141233A1 (en) Control of gene expression
BG60107B2 (bg) Метод за получаване на еукариотен полипептид
FI941947A0 (fi) Rekombinantti-core-streptavidiini
KR880010126A (ko) 펙틴 라이에이즈 발현 시스템을 암호화하는 dna 서열을 함유하는 재조합 dna 분자 및 이의 제조방법
ATE308619T1 (de) Steigerung der genexpression
DK0843731T3 (da) Adenovirusvektorer til genterapi
DK160947C (da) Gen-udtrykkelsessystem
Schwartz Genetic studies on mutant enzymes in maize, V. in vitro interconversion of allelic isozymes
ATE72837T1 (de) Vielfach amplifizierbare vektoren fuer hohe expression exogener dns.
DK558084A (da) Dna-vektorer
ATE121456T1 (de) Klonierung und überexpression von glucose-6- phosphat-dehydrogenase aus leuconostoc dextranicus.

Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
EELA Cancelled due to lapse of time