ATE287948T1 - Plasmide dna reinigung durch peg-fällung und säulen-chromatographie - Google Patents

Plasmide dna reinigung durch peg-fällung und säulen-chromatographie

Info

Publication number
ATE287948T1
ATE287948T1 AT97936375T AT97936375T ATE287948T1 AT E287948 T1 ATE287948 T1 AT E287948T1 AT 97936375 T AT97936375 T AT 97936375T AT 97936375 T AT97936375 T AT 97936375T AT E287948 T1 ATE287948 T1 AT E287948T1
Authority
AT
Austria
Prior art keywords
plasmid dna
column chromatography
dna purification
peg precipitation
dna
Prior art date
Application number
AT97936375T
Other languages
German (de)
English (en)
Inventor
Nancy Horn
Greg Budahazi
Magda Marquet
Original Assignee
Vical Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Vical Inc filed Critical Vical Inc
Application granted granted Critical
Publication of ATE287948T1 publication Critical patent/ATE287948T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
AT97936375T 1996-08-06 1997-08-05 Plasmide dna reinigung durch peg-fällung und säulen-chromatographie ATE287948T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/692,590 US5707812A (en) 1996-08-06 1996-08-06 Purification of plasmid DNA during column chromatography
PCT/US1997/013647 WO1998005767A1 (en) 1996-08-06 1997-08-05 Purification of plasmid dna by peg-precipitation and column chromatography

Publications (1)

Publication Number Publication Date
ATE287948T1 true ATE287948T1 (de) 2005-02-15

Family

ID=24781207

Family Applications (1)

Application Number Title Priority Date Filing Date
AT97936375T ATE287948T1 (de) 1996-08-06 1997-08-05 Plasmide dna reinigung durch peg-fällung und säulen-chromatographie

Country Status (7)

Country Link
US (1) US5707812A (cg-RX-API-DMAC10.html)
EP (1) EP0920497B1 (cg-RX-API-DMAC10.html)
JP (1) JP3905132B2 (cg-RX-API-DMAC10.html)
AT (1) ATE287948T1 (cg-RX-API-DMAC10.html)
CA (1) CA2262820C (cg-RX-API-DMAC10.html)
DE (1) DE69732360T2 (cg-RX-API-DMAC10.html)
WO (1) WO1998005767A1 (cg-RX-API-DMAC10.html)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US20030045492A1 (en) * 1997-08-13 2003-03-06 Tang De-Chu C. Vaccination by topical application of recombinant vectors
US6716823B1 (en) 1997-08-13 2004-04-06 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom, and uses thereof
US6348450B1 (en) 1997-08-13 2002-02-19 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom and uses thereof
US6706693B1 (en) 1997-08-13 2004-03-16 The Uab Research Foundation Vaccination by topical application of genetic vectors
US20030125278A1 (en) * 1997-08-13 2003-07-03 Tang De-Chu C. Immunization of animals by topical applications of a salmonella-based vector
US6111096A (en) 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
US7626017B2 (en) * 1997-10-31 2009-12-01 Pressure Biosciences, Inc. Pressure-enhanced extraction and purification
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
DE69922740T2 (de) 1998-05-11 2005-12-08 Tosoh Corp., Shinnanyo Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
WO1999058664A1 (en) 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
DE19916534A1 (de) * 1999-04-13 2000-10-19 Gsf Forschungszentrum Umwelt RNA-Isolierungs-Kit
US20040009936A1 (en) * 1999-05-03 2004-01-15 Tang De-Chu C. Vaccine and drug delivery by topical application of vectors and vector extracts
ATE433456T1 (de) 1999-05-28 2009-06-15 Lonza Biolog Inc Methoden zur reinigung von dns
US6617108B1 (en) 1999-07-12 2003-09-09 Technology Licensing Co. Llc Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US20020010145A1 (en) * 1999-07-12 2002-01-24 Willson Richard C. Apparatus, methods and compositions for biotechnical separations
ES2218185T3 (es) 1999-07-23 2004-11-16 Genentech, Inc. Metodo para la purificacion de adn plasmidico exento de rnasa y de solvente organico, usando filtracion de flujo tangencial.
CA2316113C (en) 1999-08-18 2008-04-01 David E. Pulleyblank Method for nucleic acid purification using iodine
US6464976B1 (en) 1999-09-07 2002-10-15 Canji, Inc. Methods and compositions for reducing immune response
US20020012990A1 (en) * 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
DE10006591B4 (de) * 2000-02-11 2007-03-29 Eppendorf Ag Verfahren zur Aufreinigung von Nukleinsäuren
PT102491B (pt) 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
US7074333B2 (en) * 2001-01-19 2006-07-11 Millipore Corporation Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration
DE10113815A1 (de) * 2001-03-21 2002-10-02 Eppendorf Ag Verfahren zur Isolierung von Plasmiden oder Proteinen aus suspendierten Bakterien- oder Hefezellen
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US20030211970A1 (en) * 2001-06-01 2003-11-13 Samuel Nochumson Processing of plasmid-containing fluids
KR100502473B1 (ko) * 2001-11-08 2005-07-20 동아제약주식회사 플라스미드 ｄｎａ의 분리방법
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법
US6833238B2 (en) * 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
US20040018559A1 (en) * 2002-07-26 2004-01-29 Applera Corporation Size-exclusion ion-exchange particles
CA2498518A1 (en) 2002-09-13 2004-03-25 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
AU2003293042A1 (en) * 2002-12-23 2004-07-29 Vical Incorporated Process for purification of plasmid dna
US7238522B2 (en) * 2003-05-30 2007-07-03 Advisys, Inc. Devices and methods for biomaterial production
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US7504243B2 (en) 2004-03-19 2009-03-17 The United States Of America As Represented By The Department Of Health And Human Services Methods for the production of biliverdin
WO2006015326A2 (en) 2004-07-30 2006-02-09 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
CN101310022B (zh) 2005-01-31 2013-01-16 默沙东公司 大规模生产质粒dna的上游和下游纯化方法
CA2597482A1 (en) * 2005-02-11 2006-08-17 Invitrogen Dynal As Method for isolating nucleic acids comprising the use of ethylene glycol multimers
US7524510B2 (en) * 2005-02-23 2009-04-28 The Uab Research Foundation Alkyl-glycoside enhanced vaccination
EP1877087B1 (en) * 2005-04-15 2013-02-20 GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Methods and compositions for producing an enhanced immune response to a human papillomavirus immunogen
WO2007065025A2 (en) * 2005-11-29 2007-06-07 Wisconsin Alumni Research Foundation Method of dna analysis using micro/nanochannel
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445441B (en) * 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
US20080300397A1 (en) * 2007-05-31 2008-12-04 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
EP2250182B1 (en) 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
WO2009111336A2 (en) * 2008-02-29 2009-09-11 Shizhong Chen Methods of purifying plasmid dna
AU2013329110B2 (en) 2012-10-12 2017-05-04 Sage Science, Inc. Side-eluting molecular fractionator
EP3447141B1 (en) 2013-03-18 2020-08-05 PreAnalytiX GmbH Stabilisation of biological samples
WO2016061416A1 (en) 2014-10-15 2016-04-21 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
US20170239658A1 (en) * 2014-10-16 2017-08-24 Ezra S. Abrams Methods and systems for controlling the shear modulus of genomic length dsdna molecules
WO2017087979A1 (en) 2015-11-20 2017-05-26 Washington University Preparative electrophoretic method for targeted purification of genomic dna fragments
EP3607308A1 (en) 2017-04-07 2020-02-12 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification
BR112021022910A2 (pt) * 2019-05-15 2022-01-25 Translate Bio Inc Métodos para purificação de rna mensageiro
CN115612684A (zh) * 2022-11-01 2023-01-17 北京启辰生生物科技有限公司 Gmp级质粒dna大规模简化生产方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01304886A (ja) * 1988-05-31 1989-12-08 Sekisui Chem Co Ltd Ｄｎａの精製方法
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA

Also Published As

Publication number Publication date
US5707812A (en) 1998-01-13
CA2262820C (en) 2007-10-23
JP2000515385A (ja) 2000-11-21
WO1998005767A1 (en) 1998-02-12
EP0920497B1 (en) 2005-01-26
EP0920497A1 (en) 1999-06-09
JP3905132B2 (ja) 2007-04-18
DE69732360T2 (de) 2006-04-06
DE69732360D1 (de) 2005-03-03
CA2262820A1 (en) 1998-02-12

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