DE69732360T2 - Plasmide dna reinigung durch peg-fällung und säulen-chromatographie - Google Patents

Plasmide dna reinigung durch peg-fällung und säulen-chromatographie Download PDF

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Publication number
DE69732360T2
DE69732360T2 DE69732360T DE69732360T DE69732360T2 DE 69732360 T2 DE69732360 T2 DE 69732360T2 DE 69732360 T DE69732360 T DE 69732360T DE 69732360 T DE69732360 T DE 69732360T DE 69732360 T2 DE69732360 T2 DE 69732360T2
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DE
Germany
Prior art keywords
plasmid dna
dna
matrix
polyethylene glycol
chromatography
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69732360T
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German (de)
English (en)
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DE69732360D1 (de
Inventor
Nancy Horn
Greg Budahazi
Magda Marquet
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Fresh Tracks Therapeutics Inc
Original Assignee
Vical Inc
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Publication date
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Publication of DE69732360D1 publication Critical patent/DE69732360D1/de
Publication of DE69732360T2 publication Critical patent/DE69732360T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
DE69732360T 1996-08-06 1997-08-05 Plasmide dna reinigung durch peg-fällung und säulen-chromatographie Expired - Lifetime DE69732360T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US08/692,590 US5707812A (en) 1996-08-06 1996-08-06 Purification of plasmid DNA during column chromatography
US692590 1996-08-06
PCT/US1997/013647 WO1998005767A1 (en) 1996-08-06 1997-08-05 Purification of plasmid dna by peg-precipitation and column chromatography

Publications (2)

Publication Number Publication Date
DE69732360D1 DE69732360D1 (de) 2005-03-03
DE69732360T2 true DE69732360T2 (de) 2006-04-06

Family

ID=24781207

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69732360T Expired - Lifetime DE69732360T2 (de) 1996-08-06 1997-08-05 Plasmide dna reinigung durch peg-fällung und säulen-chromatographie

Country Status (7)

Country Link
US (1) US5707812A (cg-RX-API-DMAC10.html)
EP (1) EP0920497B1 (cg-RX-API-DMAC10.html)
JP (1) JP3905132B2 (cg-RX-API-DMAC10.html)
AT (1) ATE287948T1 (cg-RX-API-DMAC10.html)
CA (1) CA2262820C (cg-RX-API-DMAC10.html)
DE (1) DE69732360T2 (cg-RX-API-DMAC10.html)
WO (1) WO1998005767A1 (cg-RX-API-DMAC10.html)

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US20030045492A1 (en) * 1997-08-13 2003-03-06 Tang De-Chu C. Vaccination by topical application of recombinant vectors
US6716823B1 (en) 1997-08-13 2004-04-06 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom, and uses thereof
US6348450B1 (en) 1997-08-13 2002-02-19 The Uab Research Foundation Noninvasive genetic immunization, expression products therefrom and uses thereof
US6706693B1 (en) 1997-08-13 2004-03-16 The Uab Research Foundation Vaccination by topical application of genetic vectors
US20030125278A1 (en) * 1997-08-13 2003-07-03 Tang De-Chu C. Immunization of animals by topical applications of a salmonella-based vector
US6111096A (en) 1997-10-31 2000-08-29 Bbi Bioseq, Inc. Nucleic acid isolation and purification
US7626017B2 (en) * 1997-10-31 2009-12-01 Pressure Biosciences, Inc. Pressure-enhanced extraction and purification
US6214586B1 (en) * 1997-12-08 2001-04-10 Genzyme Corporation Method for purifying plasmid DNA and plasmid DNA substantially free of genomic DNA
DE69922740T2 (de) 1998-05-11 2005-12-08 Tosoh Corp., Shinnanyo Methode zur Trennung von Nucleinsäuren mittels Flüssigchromatographie
WO1999058664A1 (en) 1998-05-14 1999-11-18 Whitehead Institute For Biomedical Research Solid phase technique for selectively isolating nucleic acids
DE19916534A1 (de) * 1999-04-13 2000-10-19 Gsf Forschungszentrum Umwelt RNA-Isolierungs-Kit
US20040009936A1 (en) * 1999-05-03 2004-01-15 Tang De-Chu C. Vaccine and drug delivery by topical application of vectors and vector extracts
ATE433456T1 (de) 1999-05-28 2009-06-15 Lonza Biolog Inc Methoden zur reinigung von dns
US6617108B1 (en) 1999-07-12 2003-09-09 Technology Licensing Co. Llc Methods and compositions for biotechnical separations using selective precipitation by compaction agents
US20020010145A1 (en) * 1999-07-12 2002-01-24 Willson Richard C. Apparatus, methods and compositions for biotechnical separations
ES2218185T3 (es) 1999-07-23 2004-11-16 Genentech, Inc. Metodo para la purificacion de adn plasmidico exento de rnasa y de solvente organico, usando filtracion de flujo tangencial.
CA2316113C (en) 1999-08-18 2008-04-01 David E. Pulleyblank Method for nucleic acid purification using iodine
US6464976B1 (en) 1999-09-07 2002-10-15 Canji, Inc. Methods and compositions for reducing immune response
US20020012990A1 (en) * 1999-12-22 2002-01-31 Lander Russel Jackson Process for the scaleable purification of plasmid DNA
DE10006591B4 (de) * 2000-02-11 2007-03-29 Eppendorf Ag Verfahren zur Aufreinigung von Nukleinsäuren
PT102491B (pt) 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
US7074333B2 (en) * 2001-01-19 2006-07-11 Millipore Corporation Recovery of linear nucleic acids by salt dilution and/or reduced pressure prior to continuous pressure differential ultrafiltration
DE10113815A1 (de) * 2001-03-21 2002-10-02 Eppendorf Ag Verfahren zur Isolierung von Plasmiden oder Proteinen aus suspendierten Bakterien- oder Hefezellen
US6406892B1 (en) 2001-05-23 2002-06-18 Bio-Rad Laboratories, Inc. Acetate-free purification of plasmid DNA on hydroxyapatite
US20030211970A1 (en) * 2001-06-01 2003-11-13 Samuel Nochumson Processing of plasmid-containing fluids
KR100502473B1 (ko) * 2001-11-08 2005-07-20 동아제약주식회사 플라스미드 ｄｎａ의 분리방법
KR100463415B1 (ko) * 2001-12-12 2004-12-23 씨제이 주식회사 세균내ｄｎａ 또는 ｒｎａ로부터 리포폴리사카라이드를제거하는 방법
US6833238B2 (en) * 2002-01-04 2004-12-21 Applera Corporation Petal-array support for use with microplates
US20040018559A1 (en) * 2002-07-26 2004-01-29 Applera Corporation Size-exclusion ion-exchange particles
CA2498518A1 (en) 2002-09-13 2004-03-25 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
AU2003293042A1 (en) * 2002-12-23 2004-07-29 Vical Incorporated Process for purification of plasmid dna
US7238522B2 (en) * 2003-05-30 2007-07-03 Advisys, Inc. Devices and methods for biomaterial production
US20060160122A1 (en) * 2004-02-18 2006-07-20 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050196856A1 (en) * 2004-02-18 2005-09-08 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US20050181378A1 (en) * 2004-02-18 2005-08-18 Applera Corporation Polyelectrolyte-coated size-exclusion ion-exchange particles
US7504243B2 (en) 2004-03-19 2009-03-17 The United States Of America As Represented By The Department Of Health And Human Services Methods for the production of biliverdin
WO2006015326A2 (en) 2004-07-30 2006-02-09 Agencourt Bioscience Corporation Methods of isolating nucleic acids using multifunctional group-coated solid phase carriers
CN101310022B (zh) 2005-01-31 2013-01-16 默沙东公司 大规模生产质粒dna的上游和下游纯化方法
CA2597482A1 (en) * 2005-02-11 2006-08-17 Invitrogen Dynal As Method for isolating nucleic acids comprising the use of ethylene glycol multimers
US7524510B2 (en) * 2005-02-23 2009-04-28 The Uab Research Foundation Alkyl-glycoside enhanced vaccination
EP1877087B1 (en) * 2005-04-15 2013-02-20 GOVERNMENT OF THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES Methods and compositions for producing an enhanced immune response to a human papillomavirus immunogen
WO2007065025A2 (en) * 2005-11-29 2007-06-07 Wisconsin Alumni Research Foundation Method of dna analysis using micro/nanochannel
GB2445442A (en) * 2006-09-26 2008-07-09 Ge Healthcare Bio Sciences Nucleic acid purification using anion exchange
GB2443505B (en) * 2006-09-26 2008-12-31 Ge Healthcare Bio Sciences Nucleic acid purification method
GB2445441B (en) * 2006-09-26 2010-06-30 Ge Healthcare Bio Sciences Nucleic acid purification method
US20080300397A1 (en) * 2007-05-31 2008-12-04 Ge Healthcare Uk Limited Modified spin column for simple and rapid plasmid dna extraction
EP2250182B1 (en) 2008-02-07 2015-12-02 GE Healthcare Bio-Sciences Corp. Isolation of dna, rna and protein from a single sample
WO2009111336A2 (en) * 2008-02-29 2009-09-11 Shizhong Chen Methods of purifying plasmid dna
AU2013329110B2 (en) 2012-10-12 2017-05-04 Sage Science, Inc. Side-eluting molecular fractionator
EP3447141B1 (en) 2013-03-18 2020-08-05 PreAnalytiX GmbH Stabilisation of biological samples
WO2016061416A1 (en) 2014-10-15 2016-04-21 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
US20170239658A1 (en) * 2014-10-16 2017-08-24 Ezra S. Abrams Methods and systems for controlling the shear modulus of genomic length dsdna molecules
WO2017087979A1 (en) 2015-11-20 2017-05-26 Washington University Preparative electrophoretic method for targeted purification of genomic dna fragments
EP3607308A1 (en) 2017-04-07 2020-02-12 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification
BR112021022910A2 (pt) * 2019-05-15 2022-01-25 Translate Bio Inc Métodos para purificação de rna mensageiro
CN115612684A (zh) * 2022-11-01 2023-01-17 北京启辰生生物科技有限公司 Gmp级质粒dna大规模简化生产方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
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JPH01304886A (ja) * 1988-05-31 1989-12-08 Sekisui Chem Co Ltd Ｄｎａの精製方法
DE4321904B4 (de) * 1993-07-01 2013-05-16 Qiagen Gmbh Verfahren zur chromatographischen Reinigung und Trennung von Nucleinsäuregemischen
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA

Also Published As

Publication number Publication date
US5707812A (en) 1998-01-13
CA2262820C (en) 2007-10-23
JP2000515385A (ja) 2000-11-21
WO1998005767A1 (en) 1998-02-12
EP0920497B1 (en) 2005-01-26
EP0920497A1 (en) 1999-06-09
JP3905132B2 (ja) 2007-04-18
DE69732360D1 (de) 2005-03-03
CA2262820A1 (en) 1998-02-12
ATE287948T1 (de) 2005-02-15

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