ATE253118T1 - Isolierungsverfahren verwendend immobilisierte proteine mit spezifischen bindungskapazitäten - Google Patents

Isolierungsverfahren verwendend immobilisierte proteine mit spezifischen bindungskapazitäten

Info

Publication number
ATE253118T1
ATE253118T1 AT94908307T AT94908307T ATE253118T1 AT E253118 T1 ATE253118 T1 AT E253118T1 AT 94908307 T AT94908307 T AT 94908307T AT 94908307 T AT94908307 T AT 94908307T AT E253118 T1 ATE253118 T1 AT E253118T1
Authority
AT
Austria
Prior art keywords
protein
alpha
pct
binding protein
yeast
Prior art date
Application number
AT94908307T
Other languages
English (en)
Inventor
Leon Gerardus J Frenken
Geus Pieter De
Franciscus Maria Klis
Holger York Toschka
Cornelis Theodorus Verrips
Original Assignee
Unilever Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Unilever Nv filed Critical Unilever Nv
Application granted granted Critical
Publication of ATE253118T1 publication Critical patent/ATE253118T1/de

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/16Enzymes or microbial cells immobilised on or in a biological cell
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62DNA sequences coding for fusion proteins
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
AT94908307T 1993-02-10 1994-02-10 Isolierungsverfahren verwendend immobilisierte proteine mit spezifischen bindungskapazitäten ATE253118T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP93200350 1993-02-10
PCT/EP1994/000427 WO1994018330A1 (en) 1993-02-10 1994-02-10 Immobilized proteins with specific binding capacities and their use in processes and products

Publications (1)

Publication Number Publication Date
ATE253118T1 true ATE253118T1 (de) 2003-11-15

Family

ID=8213623

Family Applications (1)

Application Number Title Priority Date Filing Date
AT94908307T ATE253118T1 (de) 1993-02-10 1994-02-10 Isolierungsverfahren verwendend immobilisierte proteine mit spezifischen bindungskapazitäten

Country Status (12)

Country Link
US (1) US6114147A (de)
EP (1) EP0682710B1 (de)
JP (1) JP3691055B2 (de)
AT (1) ATE253118T1 (de)
AU (1) AU6139794A (de)
DE (1) DE69433279T2 (de)
DK (1) DK0682710T3 (de)
ES (1) ES2210248T3 (de)
FI (1) FI953792A (de)
PT (1) PT682710E (de)
WO (1) WO1994018330A1 (de)
ZA (1) ZA94916B (de)

Families Citing this family (89)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5866344A (en) * 1991-11-15 1999-02-02 Board Of Regents, The University Of Texas System Antibody selection methods using cell surface expressed libraries
US20030036092A1 (en) * 1991-11-15 2003-02-20 Board Of Regents, The University Of Texas System Directed evolution of enzymes and antibodies
US5585271A (en) * 1993-02-26 1996-12-17 Sapporo Breweries Ltd. Yeast agglutination genes and yeast containing them
US5766914A (en) * 1995-01-26 1998-06-16 Michigan State University Method of producing and purifying enzymes
EP1019435A4 (de) * 1996-01-29 2001-12-12 Univ Georgetown Amplifizierung der wirksamkeit exprimierter rekombinanter proteine
US6696251B1 (en) 1996-05-31 2004-02-24 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6300065B1 (en) 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
US6699658B1 (en) 1996-05-31 2004-03-02 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
EP0879878A1 (de) * 1997-05-23 1998-11-25 Quest International B.V. Bier und andere lichtempfindliche Getränke mit verbessertem Geschmackstabilität und Verfahren zu ihrer Herstellung
EP0916726A1 (de) 1997-11-13 1999-05-19 Rijksuniversiteit te Groningen Befestigungs-Substanzen an Mikroorganismen
US6759243B2 (en) 1998-01-20 2004-07-06 Board Of Trustees Of The University Of Illinois High affinity TCR proteins and methods
EP0954978B1 (de) 1998-03-12 2011-11-30 VHsquared Limited Produkten die inaktivierte Hefen oder Schimmel enthalten, die auf ihrer Aussenoberfläche aktive Antikörper haben
ATE535154T1 (de) 1998-03-12 2011-12-15 Vhsquared Ltd Produkten die inaktivierte hefen oder schimmel enthalten, die auf ihrer aussenoberfläche aktive antikörper haben
CN1249079C (zh) * 1999-03-25 2006-04-05 瓦申泰克尼利南塔基马斯凯斯库斯公司 蛋白质的分配方法
CN100365125C (zh) * 2000-07-26 2008-01-30 韩国生命工学研究院 来源于酵母的新型细胞壁锚定蛋白及其基因和使用其的细胞表面表达系统
AU2002220275A1 (en) * 2000-12-08 2002-06-18 The Board Of Trustees Of The University Of Illinois Mutated class ii major histocompatibility proteins
WO2002048193A2 (en) * 2000-12-13 2002-06-20 Unilever N.V. Camelidae antibody arrays
JP4340068B2 (ja) * 2001-04-19 2009-10-07 関西化学機械製作株式会社 細胞表層結合性タンパク質およびその利用
CN100513548C (zh) 2001-06-11 2009-07-15 应用超微系统股份有限公司 将AcmA型蛋白质锚融合体与微生物细胞壁材料进行结合的改良方法
ATE434040T1 (de) 2001-10-01 2009-07-15 Dyax Corp Mehrkettige eukaryontische display-vektoren und deren verwendungen
WO2003064608A2 (en) * 2002-01-25 2003-08-07 The Board Of Trustees Of The Leland Stanford Junior University Surface based translation system
US20040067532A1 (en) 2002-08-12 2004-04-08 Genetastix Corporation High throughput generation and affinity maturation of humanized antibody
US8968730B2 (en) 2002-08-14 2015-03-03 Macrogenics Inc. FcγRIIB specific antibodies and methods of use thereof
US8530627B2 (en) * 2002-08-14 2013-09-10 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US8187593B2 (en) * 2002-08-14 2012-05-29 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US8193318B2 (en) * 2002-08-14 2012-06-05 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
CA2495251C (en) 2002-08-14 2018-03-06 Macrogenics, Inc. Fc.gamma.riib-specific antibodies and methods of use thereof
US8946387B2 (en) * 2002-08-14 2015-02-03 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US8044180B2 (en) * 2002-08-14 2011-10-25 Macrogenics, Inc. FcγRIIB specific antibodies and methods of use thereof
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
US7960512B2 (en) * 2003-01-09 2011-06-14 Macrogenics, Inc. Identification and engineering of antibodies with variant Fc regions and methods of using same
AU2004204494B2 (en) * 2003-01-09 2011-09-29 Macrogenics, Inc. Identification and engineering of antibodies with variant Fc regions and methods of using same
ES2393555T3 (es) 2003-10-22 2012-12-26 Keck Graduate Institute Métodos para la síntesis de polipéptidos hetero-multiméricos en levaduras usando una estrategia de apareamiento haploide.
EP1689348B1 (de) 2003-12-05 2013-05-15 Children's Hospital Medical Center Oligosaccharid-Zusammensetzungen und ihre Verwendung bei der Behandlung von Infektionen
US7521542B2 (en) 2004-05-10 2009-04-21 Macrogenics, Inc. Humanized FcγRIIB-specific antibodies and methods of use thereof
AU2005335714B2 (en) 2004-11-10 2012-07-26 Macrogenics, Inc. Engineering Fc antibody regions to confer effector function
EP1674479A1 (de) * 2004-12-22 2006-06-28 Memorial Sloan-Kettering Cancer Center Modulierung von Fc-gamma-Rezeptoren zur Optimierung einer Immunotherapie
US11254748B2 (en) 2005-04-15 2022-02-22 Macrogenics, Inc. Covalent diabodies and uses thereof
US9284375B2 (en) 2005-04-15 2016-03-15 Macrogenics, Inc. Covalent diabodies and uses thereof
ES2707152T3 (es) 2005-04-15 2019-04-02 Macrogenics Inc Diacuerpos covalentes y usos de los mismos
US9963510B2 (en) 2005-04-15 2018-05-08 Macrogenics, Inc. Covalent diabodies and uses thereof
EP1726650A1 (de) * 2005-05-27 2006-11-29 Universitätsklinikum Freiburg Monoklonale Antikörper und Einzelkettenantikörper Fragments gegen das zelloberflächen Prostataspezifische Membranantigen
JP2008546392A (ja) * 2005-06-17 2008-12-25 バイオレクシス ファーマシューティカル コーポレーション 固定されたトランスフェリン融合タンパク質ライブラリー
WO2007009082A1 (en) * 2005-07-12 2007-01-18 Codon Devices, Inc. Compositions and methods for biocatalytic engineering
EP1746103A1 (de) * 2005-07-20 2007-01-24 Applied NanoSystems B.V. Bifunktionelle Protein-Anker
DK2573114T3 (en) 2005-08-10 2016-07-04 Macrogenics Inc The identification and production of antibodies with variant Fc regions, and methods of using same
CA2644903A1 (en) * 2006-03-10 2007-09-20 Macrogenics, Inc. Identification and engineering of antibodies with variant heavy chains and methods of using same
US20070231805A1 (en) * 2006-03-31 2007-10-04 Baynes Brian M Nucleic acid assembly optimization using clamped mismatch binding proteins
EP1918302A3 (de) * 2006-05-18 2009-11-18 AvantGen, Inc. Verfahren zur Identifikation und Isolation von epitopspezifischen Antikörpern
EP2021029B1 (de) 2006-05-26 2014-06-11 MacroGenics, Inc. Humanisierte fc-gamma-riib-spezifische antikörper und verfahren zu ihrer anwendung
EP2032159B1 (de) 2006-06-26 2015-01-07 MacroGenics, Inc. Kombination aus fcgammariib-antikörpern und cd20-spezifischen antikörpern sowie verwendungsverfahren dafür
ES2599319T3 (es) 2006-06-26 2017-02-01 Macrogenics, Inc. Anticuerpos específicos de Fc RIIB y métodos de uso de éstos
US20080112961A1 (en) * 2006-10-09 2008-05-15 Macrogenics, Inc. Identification and Engineering of Antibodies with Variant Fc Regions and Methods of Using Same
WO2008140603A2 (en) 2006-12-08 2008-11-20 Macrogenics, Inc. METHODS FOR THE TREATMENT OF DISEASE USING IMMUNOGLOBULINS HAVING FC REGIONS WITH ALTERED AFFINITIES FOR FCγR ACTIVATING AND FCγR INHIBITING
JP5492563B2 (ja) 2006-12-12 2014-05-14 バイオレクシス ファーマシューティカル コーポレーション トランスフェリン融合タンパク質ライブラリー
US20110076752A1 (en) * 2007-02-09 2011-03-31 Medimmune, Llc Antibody library display by yeast cell plasma membrane
JP5754135B2 (ja) 2007-03-26 2015-07-29 アジェナス インコーポレイテッド 関心対象のタンパク質の細胞表面ディスプレイ、スクリーニング、および産生
US8877688B2 (en) 2007-09-14 2014-11-04 Adimab, Llc Rationally designed, synthetic antibody libraries and uses therefor
BRPI0816785A2 (pt) 2007-09-14 2017-05-02 Adimab Inc bibliotecas de anticorpos sintéticos racionalmente desenhadas, e, usos para as mesmas
US8637435B2 (en) * 2007-11-16 2014-01-28 Merck Sharp & Dohme Corp. Eukaryotic cell display systems
KR20190045414A (ko) 2007-11-30 2019-05-02 애브비 바이오테크놀로지 리미티드 단백질 제형 및 이의 제조방법
US8883146B2 (en) 2007-11-30 2014-11-11 Abbvie Inc. Protein formulations and methods of making same
US8795667B2 (en) 2007-12-19 2014-08-05 Macrogenics, Inc. Compositions for the prevention and treatment of smallpox
CA2715212A1 (en) 2008-03-03 2009-09-11 Glycofi, Inc. Surface display of recombinant proteins in lower eukaryotes
ITUD20080055A1 (it) * 2008-03-13 2009-09-14 Transactiva S R L Procedimento per la produzione di una proteina umana in pianta, in particolare un enzima lisosomiale umano ricombinante in endosperma di cereali
EP3045475B1 (de) 2008-04-02 2017-10-04 MacroGenics, Inc. Bcr-komplex-spezifische antikorper und verfahren zu ihrer verwendung
US8802093B2 (en) 2008-04-02 2014-08-12 Macrogenics, Inc. HER2/neu-specific antibodies and methods of using same
CA2726845C (en) * 2008-06-04 2017-09-26 Macrogenics, Inc. Antibodies with altered binding to fcrn and methods of using same
US8067339B2 (en) 2008-07-09 2011-11-29 Merck Sharp & Dohme Corp. Surface display of whole antibodies in eukaryotes
CN102245771B (zh) 2008-12-16 2013-04-10 诺瓦提斯公司 酵母展示系统
JP5898082B2 (ja) 2009-10-07 2016-04-06 マクロジェニクス,インコーポレーテッド フコシル化程度の変更により改良されたエフェクター機能を示すFc領域含有ポリペプチドおよびその使用法
US8802091B2 (en) 2010-03-04 2014-08-12 Macrogenics, Inc. Antibodies reactive with B7-H3 and uses thereof
PE20130479A1 (es) 2010-03-04 2013-05-12 Macrogenics Inc Anticuerpos reactivos con b7-h3, fragmentos inmulogicamente activos de los mismos y usos de los mismos
EP3741883B1 (de) 2010-07-16 2022-12-14 Adimab, LLC Antikörperbibliotheken
CN103154025B (zh) 2010-08-02 2015-07-01 宏观基因有限公司 共价双抗体及其用途
EP2670779B1 (de) 2011-02-01 2017-08-02 Bac Ip B.V. Gegen epitope in der ch1-domäne menschlicher igg-antikörper gerichtetes antigenbindendes protein
ES2848531T3 (es) 2011-05-17 2021-08-10 Bristol Myers Squibb Co Métodos mejorados para la selección de proteínas de unión
MX347818B (es) 2011-05-21 2017-05-15 Macrogenics Inc Dominios que enlazan suero desinmunizados y su uso para prolongar la vida media en suero.
IN2014CN03936A (de) 2011-10-24 2015-09-04 Abbvie Inc
US9487587B2 (en) 2013-03-05 2016-11-08 Macrogenics, Inc. Bispecific molecules that are immunoreactive with immune effector cells of a companion animal that express an activating receptor and cells that express B7-H3 and uses thereof
US9908938B2 (en) 2013-03-14 2018-03-06 Macrogenics, Inc. Bispecific molecules that are immunoreactive with immune effector cells that express an activating receptor and an antigen expressed by a cell infected by a virus and uses thereof
UA116479C2 (uk) 2013-08-09 2018-03-26 Макродженікс, Інк. БІСПЕЦИФІЧНЕ МОНОВАЛЕНТНЕ Fc-ДІАТІЛО, ЯКЕ ОДНОЧАСНО ЗВ'ЯЗУЄ CD32B I CD79b, ТА ЙОГО ЗАСТОСУВАННЯ
US11384149B2 (en) 2013-08-09 2022-07-12 Macrogenics, Inc. Bi-specific monovalent Fc diabodies that are capable of binding CD32B and CD79b and uses thereof
EP2839842A1 (de) 2013-08-23 2015-02-25 MacroGenics, Inc. Bispezifische monovalente Diabodies mit Fähigkeit zur Bindung von CD123 und CD3 und Verwendungen davon
EP2840091A1 (de) 2013-08-23 2015-02-25 MacroGenics, Inc. Bispezifische Diabodies, die gpA33 und CD3 binden können und Anwendungen dieser
SG11201702544WA (en) 2014-09-29 2017-04-27 Univ Duke Bispecific molecules comprising an hiv-1 envelope targeting arm
US11136613B2 (en) 2015-04-30 2021-10-05 Board Of Regents, The University Of Texas System Antibacterial polypeptide libraries and methods for screening the same
IL262396B2 (en) 2016-04-15 2023-09-01 Macrogenics Inc New b7-h3 binding molecules, drug-antibody conjugates and their uses
WO2018104893A1 (en) 2016-12-06 2018-06-14 Glaxosmithkline Intellectual Property Development Limited Alpha4-beta7 antibodies with incrased fcrn binding and/or half-life

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4859609A (en) * 1986-04-30 1989-08-22 Genentech, Inc. Novel receptors for efficient determination of ligands and their antagonists or agonists
CA1340772C (en) * 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
US5223409A (en) * 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
JPH06500470A (ja) * 1990-09-04 1994-01-20 メルク エンド カンパニー インコーポレーテッド メチロトローフ酵母細胞におけるインスリン様成長因子の産生
EP0474891A1 (de) * 1990-09-08 1992-03-18 BEHRINGWERKE Aktiengesellschaft Vektoren für die Expression malarialer Antigene an der Oberfläche von Salmonella Vakzinstämmen
SE9101433D0 (sv) * 1991-05-13 1991-05-13 Marianne Hansson Recombinant dna sequence and its use
US5223408A (en) * 1991-07-11 1993-06-29 Genentech, Inc. Method for making variant secreted proteins with altered properties
CA2139670C (en) * 1992-07-08 2005-09-27 Franciscus M. Klis Process for immobilizing enzymes to the cell wall of a microbial cell by producing a fusion protein
US5618290A (en) * 1993-10-19 1997-04-08 W.L. Gore & Associates, Inc. Endoscopic suture passer and method

Also Published As

Publication number Publication date
DE69433279T2 (de) 2004-07-15
DK0682710T3 (da) 2004-03-01
JPH08506484A (ja) 1996-07-16
FI953792A0 (fi) 1995-08-10
ZA94916B (en) 1995-08-10
EP0682710B1 (de) 2003-10-29
US6114147A (en) 2000-09-05
ES2210248T3 (es) 2004-07-01
DE69433279D1 (de) 2003-12-04
PT682710E (pt) 2004-03-31
JP3691055B2 (ja) 2005-08-31
FI953792A (fi) 1995-08-10
WO1994018330A1 (en) 1994-08-18
EP0682710A1 (de) 1995-11-22
AU6139794A (en) 1994-08-29

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