ATE183244T1 - Verfahren zum nachweis von nukleinsäuren - Google Patents

Verfahren zum nachweis von nukleinsäuren

Info

Publication number
ATE183244T1
ATE183244T1 AT96921212T AT96921212T ATE183244T1 AT E183244 T1 ATE183244 T1 AT E183244T1 AT 96921212 T AT96921212 T AT 96921212T AT 96921212 T AT96921212 T AT 96921212T AT E183244 T1 ATE183244 T1 AT E183244T1
Authority
AT
Austria
Prior art keywords
target
hybridized
nuclease
target sequence
heteroduplexes
Prior art date
Application number
AT96921212T
Other languages
English (en)
Inventor
Cassandra L Smith
Ron Yaar
Przemyslaw Szafranski
Charles R Cantor
Original Assignee
Univ Boston
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Boston filed Critical Univ Boston
Application granted granted Critical
Publication of ATE183244T1 publication Critical patent/ATE183244T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AT96921212T 1995-05-19 1996-05-20 Verfahren zum nachweis von nukleinsäuren ATE183244T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/446,102 US5753439A (en) 1995-05-19 1995-05-19 Nucleic acid detection methods

Publications (1)

Publication Number Publication Date
ATE183244T1 true ATE183244T1 (de) 1999-08-15

Family

ID=23771327

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96921212T ATE183244T1 (de) 1995-05-19 1996-05-20 Verfahren zum nachweis von nukleinsäuren

Country Status (7)

Country Link
US (1) US5753439A (de)
EP (1) EP0827551B1 (de)
AT (1) ATE183244T1 (de)
AU (1) AU6248696A (de)
CA (1) CA2221467A1 (de)
DE (1) DE69603723T2 (de)
WO (1) WO1996036731A2 (de)

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Also Published As

Publication number Publication date
DE69603723T2 (de) 2000-03-02
AU6248696A (en) 1996-11-29
EP0827551B1 (de) 1999-08-11
WO1996036731A3 (en) 1997-02-06
EP0827551A2 (de) 1998-03-11
CA2221467A1 (en) 1996-11-21
DE69603723D1 (de) 1999-09-16
WO1996036731A2 (en) 1996-11-21
US5753439A (en) 1998-05-19

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