DE69603723D1 - Verfahren zum nachweis von nukleinsäuren - Google Patents

Verfahren zum nachweis von nukleinsäuren

Info

Publication number
DE69603723D1
DE69603723D1 DE69603723T DE69603723T DE69603723D1 DE 69603723 D1 DE69603723 D1 DE 69603723D1 DE 69603723 T DE69603723 T DE 69603723T DE 69603723 T DE69603723 T DE 69603723T DE 69603723 D1 DE69603723 D1 DE 69603723D1
Authority
DE
Germany
Prior art keywords
target
hybridized
nuclease
target sequence
heteroduplexes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69603723T
Other languages
English (en)
Other versions
DE69603723T2 (de
Inventor
Cassandra Smith
Ron Yaar
Przemyslaw Szafranski
Charles Cantor
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boston University
Original Assignee
Boston University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boston University filed Critical Boston University
Application granted granted Critical
Publication of DE69603723D1 publication Critical patent/DE69603723D1/de
Publication of DE69603723T2 publication Critical patent/DE69603723T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
DE69603723T 1995-05-19 1996-05-20 Verfahren zum nachweis von nukleinsäuren Expired - Fee Related DE69603723T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/446,102 US5753439A (en) 1995-05-19 1995-05-19 Nucleic acid detection methods
PCT/US1996/006527 WO1996036731A2 (en) 1995-05-19 1996-05-20 Nucleic acid detection methods

Publications (2)

Publication Number Publication Date
DE69603723D1 true DE69603723D1 (de) 1999-09-16
DE69603723T2 DE69603723T2 (de) 2000-03-02

Family

ID=23771327

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69603723T Expired - Fee Related DE69603723T2 (de) 1995-05-19 1996-05-20 Verfahren zum nachweis von nukleinsäuren

Country Status (7)

Country Link
US (1) US5753439A (de)
EP (1) EP0827551B1 (de)
AT (1) ATE183244T1 (de)
AU (1) AU6248696A (de)
CA (1) CA2221467A1 (de)
DE (1) DE69603723T2 (de)
WO (1) WO1996036731A2 (de)

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Also Published As

Publication number Publication date
US5753439A (en) 1998-05-19
WO1996036731A3 (en) 1997-02-06
ATE183244T1 (de) 1999-08-15
DE69603723T2 (de) 2000-03-02
CA2221467A1 (en) 1996-11-21
EP0827551A2 (de) 1998-03-11
WO1996036731A2 (en) 1996-11-21
EP0827551B1 (de) 1999-08-11
AU6248696A (en) 1996-11-29

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