ATE146223T1 - Stabile d-n-alpha-carbamylase mutanten - Google Patents

Stabile d-n-alpha-carbamylase mutanten

Info

Publication number
ATE146223T1
ATE146223T1 AT95103572T AT95103572T ATE146223T1 AT E146223 T1 ATE146223 T1 AT E146223T1 AT 95103572 T AT95103572 T AT 95103572T AT 95103572 T AT95103572 T AT 95103572T AT E146223 T1 ATE146223 T1 AT E146223T1
Authority
AT
Austria
Prior art keywords
mutants
alpha
carbamylase
stable
preparation
Prior art date
Application number
AT95103572T
Other languages
English (en)
Inventor
Giuliano Galli
Renata Grifantini
Guido Grandi
Original Assignee
Eniricerche Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche Spa filed Critical Eniricerche Spa
Application granted granted Critical
Publication of ATE146223T1 publication Critical patent/ATE146223T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT95103572T 1994-04-15 1995-03-13 Stabile d-n-alpha-carbamylase mutanten ATE146223T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI940725A IT1269321B (it) 1994-04-15 1994-04-15 Mutanti stabili della d-n- -carbamilasi

Publications (1)

Publication Number Publication Date
ATE146223T1 true ATE146223T1 (de) 1996-12-15

Family

ID=11368609

Family Applications (1)

Application Number Title Priority Date Filing Date
AT95103572T ATE146223T1 (de) 1994-04-15 1995-03-13 Stabile d-n-alpha-carbamylase mutanten

Country Status (10)

Country Link
US (2) US5869298A (de)
EP (1) EP0677584B1 (de)
JP (1) JP3701341B2 (de)
AT (1) ATE146223T1 (de)
DE (1) DE69500108T2 (de)
DK (1) DK0677584T3 (de)
ES (1) ES2096494T3 (de)
GR (1) GR3022476T3 (de)
IT (1) IT1269321B (de)
SI (1) SI0677584T1 (de)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824522A (en) * 1990-12-07 1998-10-20 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Recombinant decarbamylases for producing D-α-amino acids
IT1274168B (it) * 1994-04-15 1997-07-15 Eniricerche Spa Vettore plasmidico e suo impiego per la produzione di proteine eterologhe
US5962279A (en) * 1994-06-24 1999-10-05 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Process for producing D-amino acids with composite immobilized enzyme preparation
IT1277125B1 (it) * 1995-12-21 1997-11-04 Eniricerche Spa Mutanti termostabili della d-n-alfa-carbamilasi
US6524837B1 (en) 1999-03-29 2003-02-25 California Institute Of Technology Hydantoinase variants with improved properties and their use for the production of amino acids
DE60033943T2 (de) * 1999-06-29 2007-12-20 National Institute Of Advanced Industrial Science And Technology Schwefelfreie enzyme
JP2001069981A (ja) * 1999-08-31 2001-03-21 Kanegafuchi Chem Ind Co Ltd デカルバミラーゼの立体構造およびその利用法
AU2001248278A1 (en) * 2000-04-13 2001-10-30 Novozymes A/S Extracellular expression of pectate lyase using bacillus or escherichia coli
JP4561009B2 (ja) * 2001-07-10 2010-10-13 味の素株式会社 Ｄ−ヒダントインハイドロラーゼをコードするｄｎａ、ｎ−カルバミル−ｄ−アミノ酸ハイドロラーゼをコードするｄｎａ、該遺伝子を含む組み換えｄｎａ、該組み換えｄｎａにより形質転換された細胞、該形質転換細胞を用いるタンパク質の製造方法、および、ｄ−アミノ酸の製造方法
JP4561010B2 (ja) * 2001-07-10 2010-10-13 味の素株式会社 Ｄ−ヒダントインハイドロラーゼをコードするｄｎａ、ｎ−カルバミル−ｄ−アミノ酸ハイドロラーゼをコードするｄｎａ、該遺伝子を含む組み換えｄｎａ、該組み換えｄｎａにより形質転換された細胞、該形質転換細胞を用いるタンパク質の製造方法、および、ｄ−アミノ酸の製造方法
CN115772513B (zh) * 2020-05-08 2025-05-02 江南大学 D-氨甲酰水解酶突变体、基因、表达载体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2526965B2 (ja) * 1987-02-24 1996-08-21 武田薬品工業株式会社 ムテイン，ｄｎａおよびその用途
DE69129899T2 (de) * 1990-12-07 1999-01-14 Kanegafuchi Kagaku Kogyo K.K., Osaka Verfahren zur Herstellung von D-Alpha-Aminosäuren
WO1994000577A1 (en) * 1992-06-30 1994-01-06 Smithkline Beecham P.L.C. D-n-carbamoyl-amino acid amidohydrolase and hydantoinase
ES2231772T3 (es) * 1992-08-10 2005-05-16 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Adn codante para la descarbamilasa con termoestabilidad mejorada y uso del mismo.
IT1274167B (it) * 1994-04-15 1997-07-15 Eniricerche Spa Procedimento per la produzione di d- -amminoacidi

Also Published As

Publication number Publication date
JPH0884584A (ja) 1996-04-02
JP3701341B2 (ja) 2005-09-28
US5869298A (en) 1999-02-09
US5807710A (en) 1998-09-15
EP0677584B1 (de) 1996-12-11
DE69500108D1 (de) 1997-01-23
GR3022476T3 (en) 1997-05-31
DK0677584T3 (da) 1997-05-12
DE69500108T2 (de) 1997-06-12
SI0677584T1 (en) 1997-10-31
ES2096494T3 (es) 1997-03-01
ITMI940725A0 (it) 1994-04-15
EP0677584A1 (de) 1995-10-18
ITMI940725A1 (it) 1995-10-15
IT1269321B (it) 1997-03-26

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Legal Events

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