ATE124088T1 - Reinigung von hepatitis-proteinen. - Google Patents

Reinigung von hepatitis-proteinen.

Info

Publication number
ATE124088T1
ATE124088T1 AT89106753T AT89106753T ATE124088T1 AT E124088 T1 ATE124088 T1 AT E124088T1 AT 89106753 T AT89106753 T AT 89106753T AT 89106753 T AT89106753 T AT 89106753T AT E124088 T1 ATE124088 T1 AT E124088T1
Authority
AT
Austria
Prior art keywords
hepatitis
surface antigen
subjecting
contaminant proteins
silica
Prior art date
Application number
AT89106753T
Other languages
English (en)
Inventor
William Scot Craig
Robert Steven Siegel
Original Assignee
Res Corp Technologies Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Res Corp Technologies Inc filed Critical Res Corp Technologies Inc
Application granted granted Critical
Publication of ATE124088T1 publication Critical patent/ATE124088T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2730/00—Reverse transcribing DNA viruses
    • C12N2730/00011—Details
    • C12N2730/10011—Hepadnaviridae
    • C12N2730/10111—Orthohepadnavirus, e.g. hepatitis B virus
    • C12N2730/10122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Detergent Compositions (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Physical Water Treatments (AREA)
AT89106753T 1988-04-15 1989-04-14 Reinigung von hepatitis-proteinen. ATE124088T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US07/181,934 US5004688A (en) 1988-04-15 1988-04-15 Purification of hepatitis proteins

Publications (1)

Publication Number Publication Date
ATE124088T1 true ATE124088T1 (de) 1995-07-15

Family

ID=22666418

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89106753T ATE124088T1 (de) 1988-04-15 1989-04-14 Reinigung von hepatitis-proteinen.

Country Status (14)

Country Link
US (1) US5004688A (de)
EP (1) EP0337492B1 (de)
JP (1) JP2721699B2 (de)
KR (1) KR960004707B1 (de)
AT (1) ATE124088T1 (de)
AU (1) AU608706B2 (de)
CA (1) CA1339792C (de)
DE (1) DE68923115T2 (de)
DK (1) DK181589A (de)
ES (1) ES2075006T3 (de)
IE (1) IE68408B1 (de)
IL (1) IL89924A0 (de)
MX (1) MX166707B (de)
NO (1) NO176439C (de)

Families Citing this family (42)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69013471T2 (de) * 1989-12-05 1995-03-30 Merck & Co Inc Methode zur Stabilisierung von rekombinanten Hepatitis-B-Virus-Oberflächenproteinen aus Hefe.
CU22290A1 (es) * 1990-10-08 1995-01-31 Cigb Procedimiento para la obtencion de antigeno de superficie del virus de la hepatitis b de superior capacidad inmunoganica y su uso en un preparado vacunal
WO1992013951A1 (en) * 1991-02-04 1992-08-20 The Salk Institute Biotechnology/Industrial Associates, Inc. Production of human serum albumin in methylotrophic yeast cells
US5102989A (en) * 1991-03-15 1992-04-07 Merck & Co., Inc. Method of stabilizing recombinant hepatitis B virus surface proteins from recombinant host cells
IT1248075B (it) * 1991-06-18 1995-01-05 Sclavo Spa Processo per la purificazione del virus dell'epatite a (hav), virus cosi` purificato e composizioni vaccinali che lo contengono.
RU2128707C1 (ru) * 1992-01-17 1999-04-10 Сентро Де Инженьериа Генетика И Биотекнологиа Способ получения поверхностного антигена вируса гепатита в (hbs ag), моноклональные антитела св-hepi, поверхностный антиген вируса гепатита в (hbs ag) и способ получения гибридного клона 48/1/574, продуцирующего моноклональные антитела св-hepi
US6362003B1 (en) 1992-02-24 2002-03-26 Coulter Corporation Hematological reference control composition containing leukocyte analogs, methods of making, and uses thereof
US5494801A (en) * 1993-12-03 1996-02-27 Biostar, Inc. Microorganism antigen extraction methods
US5451660A (en) * 1993-12-13 1995-09-19 Genentech, Inc. Method for purifying polypeptides
HRP950097A2 (en) 1994-03-08 1997-06-30 Merck & Co Inc Hepatitis a virus culture process
US5863894A (en) * 1995-06-05 1999-01-26 Corvas International, Inc. Nematode-extracted anticoagulant protein
CA2202351A1 (en) * 1994-10-18 1996-04-25 George Phillip Vlasuk Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5866543A (en) * 1995-06-05 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5872098A (en) * 1995-06-05 1999-02-16 Corvas International, Inc. Nematode-extracted anticoagulant protein
NZ296648A (en) * 1994-10-18 2001-05-25 Corvas Int Inc Nematode-extracted serine protease inhibitors and anticoagulant proteins
US5866542A (en) * 1994-10-18 1999-02-02 Corvas International, Inc. Nematode-extracted anticoagulant protein
US5945275A (en) * 1994-10-18 1999-08-31 Corvas International, Inc. Nematode-extracted anticoagulant protein
KR960023066A (ko) * 1994-12-10 1996-07-18 성재갑 전에스 (s) 2 펩티드 함유 비 (b) 형 간염 표면항원의 정제 방법
MX9605082A (es) 1996-10-24 1998-04-30 Univ Autonoma De Nuevo Leon Levaduras metilotroficas modificadas geneticamente para la produccion y secrecion de hormona de crecimiento humano.
KR100251015B1 (ko) * 1997-06-05 2000-05-01 성재갑 B형간염표면항원의정제방법
DE19918619A1 (de) * 1999-04-23 2000-10-26 Rhein Biotech Proz & Prod Gmbh Verfahren zum Gewinnen von rekombinantem HBsAg
US7625756B2 (en) 2000-06-28 2009-12-01 GycoFi, Inc. Expression of class 2 mannosidase and class III mannosidase in lower eukaryotic cells
WO2002000879A2 (en) * 2000-06-28 2002-01-03 Glycofi, Inc. Methods for producing modified glycoproteins
US7449308B2 (en) * 2000-06-28 2008-11-11 Glycofi, Inc. Combinatorial DNA library for producing modified N-glycans in lower eukaryotes
US7598055B2 (en) * 2000-06-28 2009-10-06 Glycofi, Inc. N-acetylglucosaminyltransferase III expression in lower eukaryotes
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US8697394B2 (en) * 2000-06-28 2014-04-15 Glycofi, Inc. Production of modified glycoproteins having multiple antennary structures
JP4798833B2 (ja) * 2000-10-24 2011-10-19 一般財団法人化学及血清療法研究所 加熱処理工程を含むヒト血清アルブミンの製造方法
KR100405174B1 (ko) * 2001-02-02 2003-11-12 씨제이 주식회사 재조합 β형 간염바이러스 표면항원의 정제방법
KR100436655B1 (ko) * 2001-07-25 2004-06-22 (주)엘피스바이오텍 광범위한 단백질에 적용할 수 있는 단백질의 농축 및정제 방법
US7118901B2 (en) * 2002-12-18 2006-10-10 Roche Diagnostics Operations, Inc. Recombinant bovine pancreatic desoxyribonuclease I with high specific activity
DE60319333D1 (de) * 2002-12-20 2008-04-10 Roche Diagnostics Gmbh Hitzelabile Desoxyribonuklease I-Varianten
US7332299B2 (en) * 2003-02-20 2008-02-19 Glycofi, Inc. Endomannosidases in the modification of glycoproteins in eukaryotes
EP1460425A1 (de) 2003-03-17 2004-09-22 Boehringer Mannheim Gmbh Entglykosilierte Enzymen für Konjugaten
DE602004025192D1 (de) * 2003-12-05 2010-03-11 Roche Diagnostics Gmbh Rekombinante Carboxypeptidase B und ihre Aufreinigung
CA2548378A1 (en) * 2003-12-30 2005-07-14 Bharat Biotech International Limited A process for the preparation and purification of recombinant proteins
CA2621344C (en) * 2005-09-14 2014-12-16 Sanofi-Aventis Deutschland Gmbh Cleavage of precursors of insulins by a variant of trypsin
AU2013330344B2 (en) 2012-09-17 2018-07-05 W. R. Grace & Co.-Conn. Chromatography media and devices
WO2015080943A1 (en) 2013-11-26 2015-06-04 Yale University Novel cell-penetrating compositions and methods using same
JP6914189B2 (ja) 2014-05-02 2021-08-04 ダブリュー・アール・グレース・アンド・カンパニー−コーンＷ Ｒ Ｇｒａｃｅ ＆ Ｃｏ−Ｃｏｎｎ 官能化担体材料並びに官能化担体材料を作製及び使用する方法
EP3302784B1 (de) 2015-06-05 2021-10-06 W.R. Grace & Co.-Conn. Adsorbierende klärmittel für bioprozesstechnik und verfahren zur herstellung und verwendung davon
CN108047316B (zh) * 2018-01-04 2021-06-15 远大赛威信生命科学(南京)有限公司 重组乙肝核心抗原的分离纯化方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4515714A (en) * 1983-03-09 1985-05-07 Juridicial Foundation, The Chemo-Semo-Sero-Therapeutic Research Institute Method for purification of hepatitis B virus surface antigen
US4533596A (en) * 1983-06-28 1985-08-06 Ncr Corporation Thermal magnetic transfer ribbon
JPS6078999A (ja) * 1983-10-05 1985-05-04 Chemo Sero Therapeut Res Inst ＨＢｓ抗原の精製方法
JPS60197629A (ja) * 1984-03-16 1985-10-07 Chemo Sero Therapeut Res Inst ＨＢｓ抗原の精製方法
US4624918A (en) * 1984-07-09 1986-11-25 Genentech, Inc. Purification process for hepatitis surface antigen and product thereof
CA1268437A (en) * 1984-12-21 1990-05-01 Haruo Fujita Method for purification of hbc antigen and method for measurement of hbc antibody by using said purified hbc antigen
US4649192A (en) * 1985-05-30 1987-03-10 Smith Kline-Rit Method for the isolation and purification of hepatitis B surface antigen using polysorbate
US4895800A (en) * 1985-11-26 1990-01-23 Phillips Petroleum Company Yeast production of hepatitis B surface antigen
US4683293A (en) * 1986-10-20 1987-07-28 Phillips Petroleum Company Purification of pichia produced lipophilic proteins

Also Published As

Publication number Publication date
IE68408B1 (en) 1996-06-12
EP0337492B1 (de) 1995-06-21
DK181589A (da) 1989-10-16
ES2075006T3 (es) 1995-10-01
KR960004707B1 (ko) 1996-04-12
NO176439B (no) 1994-12-27
MX166707B (es) 1993-01-28
IE891206L (en) 1989-10-15
NO176439C (no) 1995-04-05
JP2721699B2 (ja) 1998-03-04
DK181589D0 (da) 1989-04-14
AU608706B2 (en) 1991-04-11
JPH01311028A (ja) 1989-12-15
DE68923115T2 (de) 1995-11-02
DE68923115D1 (de) 1995-07-27
CA1339792C (en) 1998-03-31
IL89924A0 (en) 1989-12-15
US5004688A (en) 1991-04-02
KR890016166A (ko) 1989-11-28
NO891532D0 (no) 1989-04-13
NO891532L (no) 1989-10-16
EP0337492A1 (de) 1989-10-18
AU3277989A (en) 1989-10-19

Similar Documents

Publication Publication Date Title
ATE124088T1 (de) Reinigung von hepatitis-proteinen.
DE68915675D1 (de) Verfahren zur Reinigung von Vitamin K-abhängigen Proteinen.
MX9603637A (es) Purificacion de anticuerpo.
ATE274522T1 (de) Abtrennung von antikörper-monomeren von deren multimeren mittels ionaustausch-chromatographie
Bayer et al. Enzyme-based detection of glycoproteins on blot transfers using avidin-biotin technology
DE3751389D1 (de) Verfahren zur Trennung von Proteinen.
FI19992546A (fi) Hepatiitti B:n S- ja PreS2-proteiinien ilmentäminen metylotrofisissa hiivoissa
ATE30844T1 (de) Verfahren zur herstellung der wichtigsten proteine des haemolysierten bluts in einer nichtdenaturierten form.
DE3887750D1 (de) Selektive entfernung von immunkomplexen.
Piantelli et al. Echinococcus granulosus: identification of subunits of the major antigens
CA2031354A1 (en) Method of stabilizing recombinant hepatitis b virus surface proteins from yeast
ATE166062T1 (de) Verfahren zur reinigung des big-endothelin proteins
Bacon et al. Resolution of pea legumin subunits by high-performance liquid chromatography
DE3788170D1 (de) Methode zur Reinigung und Isolierung von zwei ionischen Formen von Somatomedin.
US3687927A (en) Orgotein isolation process employing single ion exchange resin having both acidic and basic groups
DE3774086D1 (de) Laktoferrin enthaltendes inkubationsmedium fuer festphasen-immunometrische verfahren und seine verwendung.
Hoag et al. Optimal conditions for isolating human placental alkaline phosphatase by immunosorption
ATE33842T1 (de) Immuninterferon und verfahren zu seiner reinigung.
LI et al. The Application of Electrofocusing to the Fractionation of Proteins
KR950008687A (ko) 인간 백혈구 인터페론 α-2 재조합 발현벡터 및 형질전환체
KR940014805A (ko) 신규한 아미노펩티다제 및 그의 분리방법

Legal Events

Date Code Title Description
REN Ceased due to non-payment of the annual fee