AR112783A1 - Imagen por enfoque isoléctrico en capilar para analizar variantes de proteínas en una matriz de muestra - Google Patents
Imagen por enfoque isoléctrico en capilar para analizar variantes de proteínas en una matriz de muestraInfo
- Publication number
- AR112783A1 AR112783A1 ARP180102365A ARP180102365A AR112783A1 AR 112783 A1 AR112783 A1 AR 112783A1 AR P180102365 A ARP180102365 A AR P180102365A AR P180102365 A ARP180102365 A AR P180102365A AR 112783 A1 AR112783 A1 AR 112783A1
- Authority
- AR
- Argentina
- Prior art keywords
- capillary
- charge
- variants
- protein
- vegf
- Prior art date
Links
- 108090000623 proteins and genes Proteins 0.000 title abstract 5
- 102000004169 proteins and genes Human genes 0.000 title abstract 5
- 239000011159 matrix material Substances 0.000 title 1
- 102000001708 Protein Isoforms Human genes 0.000 abstract 3
- 108010029485 Protein Isoforms Proteins 0.000 abstract 3
- 108010081667 aflibercept Proteins 0.000 abstract 3
- 239000000872 buffer Substances 0.000 abstract 3
- 239000000654 additive Substances 0.000 abstract 2
- 230000000996 additive effect Effects 0.000 abstract 2
- 238000003556 assay Methods 0.000 abstract 2
- 238000000034 method Methods 0.000 abstract 2
- 229920000609 methyl cellulose Polymers 0.000 abstract 2
- 239000001923 methylcellulose Substances 0.000 abstract 2
- 239000000203 mixture Substances 0.000 abstract 2
- 230000007935 neutral effect Effects 0.000 abstract 2
- 230000000087 stabilizing effect Effects 0.000 abstract 2
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 abstract 1
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 abstract 1
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 abstract 1
- 238000002835 absorbance Methods 0.000 abstract 1
- 230000002378 acidificating effect Effects 0.000 abstract 1
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- NBVXSUQYWXRMNV-UHFFFAOYSA-N fluoromethane Chemical compound FC NBVXSUQYWXRMNV-UHFFFAOYSA-N 0.000 abstract 1
- 238000013508 migration Methods 0.000 abstract 1
- 230000005012 migration Effects 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
- C07K1/26—Electrophoresis
- C07K1/28—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44795—Isoelectric focusing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/60—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrostatic variables, e.g. electrographic flaw testing
Abstract
Reivindicación 1: Un método para analizar variantes de carga del factor de crecimiento endotelial vascular VEGF-Trap, que comprende: cargar una muestra de proteína en un capilar de separación que comprende una mezcla de al menos un anfolito portador, metilcelulosa y un aditivo estabilizante; aplicar un primer voltaje durante un primer período de tiempo predeterminado de manera que el anfolito portador forme un gradiente de pH dentro del capilar; aplicar un segundo voltaje durante un segundo período de tiempo predeterminado para enfocar la migración de las variantes de carga de la proteína dentro del capilar de manera que la carga general de las variantes sea neutra; y detectar y cuantificar las variantes de carga de la proteína. Reivindicación 2: El método de la reivindicación 1, en donde: la detección y cuantificación de variantes de carga comprende: medir la absorbancia para una pluralidad de isoformas de variantes de carga; segregar la pluralidad de isoformas de variantes de carga en regiones aisladas que comprenden al menos una primera región ácida (R1), una segunda región neutra (R2) y una tercera región alcalina (R3); y determinar un porcentaje de isoformas de variantes de carga que caen dentro de cada una de las regiones R1, R2 y R3. Reivindicación 20: Un tubo capilar de iCIEF configurado para su uso en un análisis de variantes de carga de VEGF-Trap, que comprende: un tubo capilar configurado para recibir una proteína, y configurado con una mezcla de anfolito portador, metilcelulosa y un aditivo estabilizante, en donde el tubo capilar incluye un revestimiento de fluorocarbono. Reivindicación 22: Un kit de iCIEF configurado para su uso en un análisis de variantes de carga de VEGF-Trap, que comprende uno o más tubos capilares de acuerdo con cualquiera de las reivindicaciones 19 - 20.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762547602P | 2017-08-18 | 2017-08-18 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR112783A1 true AR112783A1 (es) | 2019-12-11 |
Family
ID=65361327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP180102365A AR112783A1 (es) | 2017-08-18 | 2018-08-17 | Imagen por enfoque isoléctrico en capilar para analizar variantes de proteínas en una matriz de muestra |
Country Status (14)
| Country | Link |
|---|---|
| US (2) | US20190056350A1 (es) |
| EP (1) | EP3668546A4 (es) |
| JP (2) | JP7399845B2 (es) |
| KR (2) | KR20230141948A (es) |
| CN (1) | CN111132695A (es) |
| AR (1) | AR112783A1 (es) |
| AU (1) | AU2018317438A1 (es) |
| CA (1) | CA3065562A1 (es) |
| EA (1) | EA202090515A1 (es) |
| IL (1) | IL271237A (es) |
| MX (1) | MX2020001818A (es) |
| SG (1) | SG11201911919PA (es) |
| TW (2) | TWI774827B (es) |
| WO (1) | WO2019036604A1 (es) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4042151A1 (en) * | 2019-10-10 | 2022-08-17 | Regeneron Pharmaceuticals, Inc. | Liquid chromatography-mass spectrometry (lc-ms) methods for analyzing ampholyte lot variation |
| US20230296559A1 (en) * | 2022-03-18 | 2023-09-21 | Regeneron Pharmaceuticals, Inc. | Methods and systems for analyzing polypeptide variants |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR100958165B1 (ko) * | 2008-05-26 | 2010-05-14 | 연세대학교 산학협력단 | 비젤방식의 2차원 단백질분리용 다중채널장치 |
| FR2947632B1 (fr) * | 2009-07-01 | 2011-11-11 | Sebia Sa | Analyse et dosage d'hemoglobines glyquees par electrophorese capillaire, compositions tampon et kits pour electrophorese capillaire |
| HUE042192T2 (hu) * | 2011-09-23 | 2019-06-28 | Oncomed Pharm Inc | VEGF/DLL4-kötõ ágensek és alkalmazásaik |
| EP2844373A4 (en) * | 2012-05-03 | 2015-12-16 | Medimmune Llc | METHOD FOR ANALYZING SAMPLE COMPONENTS |
| KR102637699B1 (ko) * | 2014-10-24 | 2024-02-19 | 브리스톨-마이어스 스큅 컴퍼니 | 변형된 fgf-21 폴리펩티드 및 그의 용도 |
| WO2016070062A2 (en) * | 2014-10-31 | 2016-05-06 | Genentech, Inc. | Anti-il-17a and il-17f cross reactive antibody variants and compositions comprising and methods of making and using same |
| US20160144025A1 (en) * | 2014-11-25 | 2016-05-26 | Regeneron Pharmaceuticals, Inc. | Methods and formulations for treating vascular eye diseases |
| HUE050894T2 (hu) * | 2015-04-17 | 2021-01-28 | Bristol Myers Squibb Co | Kompozíciók, amelyek tartalmazzák ipilimumab és nivolumab kombinációját |
| AU2016358111B2 (en) * | 2015-11-18 | 2021-11-18 | Formycon Ag | Pre-filled pharmaceutical package comprising a liquid formulation of a VEGF-antagonist |
| PL3389636T3 (pl) * | 2015-12-16 | 2022-11-28 | Regeneron Pharmaceuticals, Inc. | Kompozycje i metody wytwarzania mikrocząstek białkowych |
-
2018
- 2018-08-17 KR KR1020237033115A patent/KR20230141948A/ko not_active Application Discontinuation
- 2018-08-17 WO PCT/US2018/046893 patent/WO2019036604A1/en unknown
- 2018-08-17 CA CA3065562A patent/CA3065562A1/en active Pending
- 2018-08-17 MX MX2020001818A patent/MX2020001818A/es unknown
- 2018-08-17 KR KR1020207000244A patent/KR102584848B1/ko active IP Right Grant
- 2018-08-17 EP EP18845630.5A patent/EP3668546A4/en active Pending
- 2018-08-17 AU AU2018317438A patent/AU2018317438A1/en active Pending
- 2018-08-17 EA EA202090515A patent/EA202090515A1/ru unknown
- 2018-08-17 TW TW107128788A patent/TWI774827B/zh active
- 2018-08-17 SG SG11201911919PA patent/SG11201911919PA/en unknown
- 2018-08-17 JP JP2020502363A patent/JP7399845B2/ja active Active
- 2018-08-17 AR ARP180102365A patent/AR112783A1/es unknown
- 2018-08-17 US US16/104,355 patent/US20190056350A1/en not_active Abandoned
- 2018-08-17 TW TW111126023A patent/TW202242403A/zh unknown
- 2018-08-17 CN CN201880053645.8A patent/CN111132695A/zh active Pending
-
2019
- 2019-12-08 IL IL271237A patent/IL271237A/en unknown
-
2022
- 2022-06-10 US US17/837,550 patent/US20220317088A1/en active Pending
-
2023
- 2023-07-07 JP JP2023112421A patent/JP2023145494A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019036604A1 (en) | 2019-02-21 |
| TWI774827B (zh) | 2022-08-21 |
| EA202090515A1 (ru) | 2020-05-26 |
| KR20200078466A (ko) | 2020-07-01 |
| KR20230141948A (ko) | 2023-10-10 |
| JP7399845B2 (ja) | 2023-12-18 |
| JP2023145494A (ja) | 2023-10-11 |
| EP3668546A1 (en) | 2020-06-24 |
| TW201920927A (zh) | 2019-06-01 |
| MX2020001818A (es) | 2020-07-13 |
| JP2020531802A (ja) | 2020-11-05 |
| WO2019036604A9 (en) | 2019-04-04 |
| US20220317088A1 (en) | 2022-10-06 |
| CA3065562A1 (en) | 2019-02-21 |
| SG11201911919PA (en) | 2020-02-27 |
| CN111132695A (zh) | 2020-05-08 |
| EP3668546A4 (en) | 2021-05-12 |
| AU2018317438A1 (en) | 2020-01-16 |
| IL271237A (en) | 2020-01-30 |
| TW202242403A (zh) | 2022-11-01 |
| US20190056350A1 (en) | 2019-02-21 |
| KR102584848B1 (ko) | 2023-10-04 |
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