AR112543A1 - Microorganismos para la producción de feromonas de insectos y compuestos relacionados - Google Patents

Microorganismos para la producción de feromonas de insectos y compuestos relacionados

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Publication number
AR112543A1
AR112543A1 ARP180101321A AR112543A1 AR 112543 A1 AR112543 A1 AR 112543A1 AR P180101321 A ARP180101321 A AR P180101321A AR 112543 A1 AR112543 A1 AR 112543A1
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AR
Argentina
Prior art keywords
aldehydes
acetates
fatty alcohols
production
useful
Prior art date
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English (en)
Inventor
Effendi Leonard
Pedro Coelho
Peter Meinhold
Thomas Heel
Micah Sheppard
Keith Wampler
Original Assignee
Provivi Inc
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Publication date
Application filed by Provivi Inc filed Critical Provivi Inc
Publication of AR112543A1 publication Critical patent/AR112543A1/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/19—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water (1.14.19)
    • C12Y114/19001—Stearoyl-CoA 9-desaturase (1.14.19.1), i.e. DELTA9-desaturase

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Mycology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente solicitud se relaciona con microorganismos recombinantes útiles en la biosíntesis de alcoholes grasos C₆₋₂₄, aldehídos y acetatos insaturados que pueden ser útiles como feromonas de insectos, fragancias, saborizantes e intermediarios poliméricos. Los microorganismos recombinantes pueden expresar enzimas o variantes de enzimas útiles para la producción de alcoholes grasos C₆₋₂₄, aldehídos y acetatos insaturados y/o pueden ser modificados para regular hacia abajo las vías a fin de desviar la producción hacia dichos alcoholes grasos C₆₋₂₄, aldehídos y acetatos insaturados. Los alcoholes grasos C₆₋₂₄, aldehídos y acetatos descritos en la presente se pueden usar como sustratos para reacciones de metátesis para expandir el repertorio de compuestos y feromonas destinados. La solicitud además se relaciona con microorganismos recombinantes que co-expresan una vía de feromonas y una vía para la producción de una proteína, péptido, oligonucleótido o pequeña molécula tóxicos adecuados para uso en un enfoque de control de plagas del tipo atraer y exterminar. La solicitud además se relaciona con microorganismos modificados para expresar o regular hacia abajo enzimas útiles para la producción de alcoholes grasos, aldehídos y acetatos de cadena corta insaturados que pueden ser útiles como feromonas de insectos, fragancias, saborizantes e intermediarios poliméricos. También se proveen métodos para producir alcoholes grasos C₆₋₂₄, aldehídos y acetatos insaturados usando los microorganismos recombinantes, así como también composiciones que comprenden los microorganismos recombinantes y/u opcionalmente uno o más de los alcoholes grasos, aldehídos o acetatos.
ARP180101321 2017-05-17 2018-05-17 Microorganismos para la producción de feromonas de insectos y compuestos relacionados AR112543A1 (es)

Applications Claiming Priority (1)

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US201762507654P 2017-05-17 2017-05-17

Publications (1)

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AR112543A1 true AR112543A1 (es) 2019-11-13

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Country Status (8)

Country Link
US (2) US11104921B2 (es)
EP (1) EP3635124A4 (es)
JP (1) JP7216018B2 (es)
CN (1) CN110914442A (es)
AR (1) AR112543A1 (es)
BR (1) BR112019024258A2 (es)
MX (1) MX2019013671A (es)
WO (1) WO2018213554A1 (es)

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AR112543A1 (es) * 2017-05-17 2019-11-13 Provivi Inc Microorganismos para la producción de feromonas de insectos y compuestos relacionados
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EP4085140A1 (en) * 2019-12-11 2022-11-09 Provivi, Inc. Biosynthesis of insect pheromones and precursors thereof
CN110938579A (zh) * 2019-12-13 2020-03-31 杭州唯铂莱生物科技有限公司 酪氨酸解氨酶重组菌、酪氨酸解氨酶及其制备方法和应用
IL293960A (en) * 2019-12-20 2022-08-01 Biophero Aps Yeast cells and methods for the production of e10,e8-dodecadienyl coenzyme a, codelmon and its derivatives
US20210254092A1 (en) * 2020-02-07 2021-08-19 Board Of Regents Of The University Of Nebraska Methods of Producing Insect Pheromones
WO2021262906A1 (en) * 2020-06-23 2021-12-30 Provivi, Inc. Synthesis of complex insect pheromone blends and reaction apparatuses for the same
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