AR094716A1 - Utilización de una cepa procariótica de auxotrofía para aminoácidos revertida para la producción recombinante de un polipéptido - Google Patents
Utilización de una cepa procariótica de auxotrofía para aminoácidos revertida para la producción recombinante de un polipéptidoInfo
- Publication number
- AR094716A1 AR094716A1 ARP140100531A ARP140100531A AR094716A1 AR 094716 A1 AR094716 A1 AR 094716A1 AR P140100531 A ARP140100531 A AR P140100531A AR P140100531 A ARP140100531 A AR P140100531A AR 094716 A1 AR094716 A1 AR 094716A1
- Authority
- AR
- Argentina
- Prior art keywords
- prokaryotic cell
- auxotrophy
- polypeptide
- amino acids
- growth medium
- Prior art date
Links
- 150000001413 amino acids Chemical class 0.000 title abstract 5
- 229920001184 polypeptide Polymers 0.000 title abstract 4
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 4
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 210000001236 prokaryotic cell Anatomy 0.000 abstract 8
- 239000001963 growth medium Substances 0.000 abstract 4
- 210000004027 cell Anatomy 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000002609 medium Substances 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 210000001322 periplasm Anatomy 0.000 abstract 1
- 230000009469 supplementation Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
En la presente se describe un método para producir un polipéptido en una célula procariótica, que comprende el paso de cultivar una célula procariótica que comprende uno o más ácidos nucleicos que codifican al polipéptido, en un medio de crecimiento mínimo definido químicamente, y recuperar el polipéptido a partir de la célula procariótica o del periplasma de la célula procariótica o del medio, donde la célula procariótica es una célula procariótica de auxotrofia para aminoácidos revertida, donde el crecimiento de la célula procariótica de auxotrofía para aminoácidos revertida, en comparación con la célula procariótica no reparada, en las mismas condiciones de cultivo y en el mismo medio de crecimiento, requiere la suplementación de menos aminoácidos al medio de crecimiento, y donde el medio de crecimiento mínimo definido químicamente está libre del aminoácido que corresponde a la auxotrofía que se ha reparado en la célula procariótica de auxotrofía revertida.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13156288 | 2013-02-22 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR094716A1 true AR094716A1 (es) | 2015-08-19 |
Family
ID=47749683
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP140100531A AR094716A1 (es) | 2013-02-22 | 2014-02-20 | Utilización de una cepa procariótica de auxotrofía para aminoácidos revertida para la producción recombinante de un polipéptido |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10155970B2 (es) |
| EP (1) | EP2959006B1 (es) |
| JP (1) | JP6412509B2 (es) |
| CN (2) | CN110541018A (es) |
| AR (1) | AR094716A1 (es) |
| ES (1) | ES2688044T3 (es) |
| HR (1) | HRP20181414T1 (es) |
| PL (1) | PL2959006T3 (es) |
| SG (1) | SG11201505916XA (es) |
| SI (1) | SI2959006T1 (es) |
| TW (1) | TWI628281B (es) |
| WO (1) | WO2014128135A1 (es) |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5932439A (en) | 1995-11-13 | 1999-08-03 | Monsanto Comapny | Escherichia coli K-12 strains for production of recombinant proteins |
| ES2337027T3 (es) | 1997-12-05 | 2010-04-20 | Europaisches Laboratorium Fur Molekularbiologie (Embl) | Nuevo metodo de clonacion de dna basado en el sistema de recombinacion rece-rect de e. coli. |
| US6291245B1 (en) * | 1998-07-15 | 2001-09-18 | Roche Diagnostics Gmbh | Host-vector system |
| EP0972838B1 (en) | 1998-07-15 | 2004-09-15 | Roche Diagnostics GmbH | Escherichia coli host/vector system based on antibiotic-free selection by complementation of an auxotrophy |
| JP2000189162A (ja) * | 1998-12-25 | 2000-07-11 | Olympus Optical Co Ltd | 変異原検出法およびそれに用いる形質転換体 |
| US6355412B1 (en) | 1999-07-09 | 2002-03-12 | The European Molecular Biology Laboratory | Methods and compositions for directed cloning and subcloning using homologous recombination |
| CA2443365C (en) | 2002-11-19 | 2010-01-12 | F. Hoffmann-La Roche Ag | Methods for the recombinant production of antifusogenic peptides |
| ATE312939T1 (de) | 2003-06-12 | 2005-12-15 | Verfahren zur rekombinanten herstellung von polypeptiden | |
| ES2558751T3 (es) | 2010-08-30 | 2016-02-08 | F. Hoffmann-La Roche Ag | Alimento alcalino |
| KR101631363B1 (ko) * | 2010-08-30 | 2016-06-17 | 에프. 호프만-라 로슈 아게 | 테트라넥틴 아포지방단백질 a-i, 이를 함유하는 지질 입자 및 이의 용도 |
| EP2611921A2 (en) * | 2010-08-30 | 2013-07-10 | F.Hoffmann-La Roche Ag | Prokaryotic expression construct |
-
2014
- 2014-02-19 ES ES14706005.7T patent/ES2688044T3/es active Active
- 2014-02-19 EP EP14706005.7A patent/EP2959006B1/en active Active
- 2014-02-19 CN CN201910857484.4A patent/CN110541018A/zh active Pending
- 2014-02-19 SI SI201430869T patent/SI2959006T1/sl unknown
- 2014-02-19 SG SG11201505916XA patent/SG11201505916XA/en unknown
- 2014-02-19 CN CN201480009903.4A patent/CN105026575A/zh active Pending
- 2014-02-19 US US14/769,594 patent/US10155970B2/en active Active
- 2014-02-19 JP JP2015558428A patent/JP6412509B2/ja active Active
- 2014-02-19 PL PL14706005T patent/PL2959006T3/pl unknown
- 2014-02-19 WO PCT/EP2014/053171 patent/WO2014128135A1/en active Application Filing
- 2014-02-20 AR ARP140100531A patent/AR094716A1/es unknown
- 2014-02-21 TW TW103105960A patent/TWI628281B/zh active
-
2018
- 2018-09-03 HR HRP20181414TT patent/HRP20181414T1/hr unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN105026575A (zh) | 2015-11-04 |
| TW201444975A (zh) | 2014-12-01 |
| CN110541018A (zh) | 2019-12-06 |
| US10155970B2 (en) | 2018-12-18 |
| WO2014128135A1 (en) | 2014-08-28 |
| JP2016507243A (ja) | 2016-03-10 |
| HRP20181414T1 (hr) | 2018-10-19 |
| ES2688044T3 (es) | 2018-10-30 |
| EP2959006B1 (en) | 2018-07-18 |
| JP6412509B2 (ja) | 2018-10-24 |
| PL2959006T3 (pl) | 2018-11-30 |
| EP2959006A1 (en) | 2015-12-30 |
| TWI628281B (zh) | 2018-07-01 |
| SI2959006T1 (sl) | 2018-10-30 |
| US20150376674A1 (en) | 2015-12-31 |
| SG11201505916XA (en) | 2015-09-29 |
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| Date | Code | Title | Description |
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| FB | Suspension of granting procedure |